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Rajkot, India

The Saurashtra University is one of the significant universities in Gujarat state in India. This university was established on 23 May 1967 in Rajkot city, the administrative headquarters at Rajkot. Presently, 272 colleges in Amreli, Jamnagar, Junagadh, Porbandar, Rajkot and Surendranagar districts are affiliated with the Saurashtra University. Wikipedia.

The protective effect of Lactobacillus rhamnosus 231 (Lr 231) against potent carcinogen N-Methyl-N'-Nitro-N-Nitrosoguanidine (MNNG) in the rat model is studied. Daily feeding with Lr 231 improved the body weight of male Wistar rats compared with control groups. Fecal azoreductase (p < 0.001) and nitroreductase (p < 0.01) enzyme activity decreased significantly in Lr 231 group in comparison with control groups that received only phosphate buffer or MNNG. Oral administration of MNNG led to a significant increase in Glutathione transferase (GST) while Glutathione reductase (GSH) showed decreased activity. Conversely, feeding Lr 231 showed significantly increased GSH and decreased GST activity in comparison to the MNNG group, emphasizing the protection provided by Lr 231 against MNNG. Histopathological analysis of liver, spleen and colon showed decreased signs of inflammation in the Lr 231 group. The present study highlights that inclusion of active Lr 231 in regular diets could be used to prevent MNNG induced colon carcinoma. Source

Kumar S.,Saurashtra University
International Journal of Pharma and Bio Sciences

In this study, chlorpyrifos degrading capability of four bacterial monocultures (RCC-2, GCC-1, GCC-3 and JCC-3) and two bacterial mixed-cultures (GCE345 and GCC134) was investigated in terms of treatment duration and culture volume, using soil slurry medium. Among the bacterial mono-cultures, RCC-2 was found to be most efficient with 21, 37, 54 and 77% of chlorpyrifos degradation in 5, 10, 15 and 30 days of treatment duration, respectively. Out of two bacterial mixed-cultures, GCC134 was more effective and resulted in 24, 38, 56 and 85% degradation of chlorpyrifos in 5, 10, 15 and 30 days of treatment, respectively. Chlorpyrifos degradation was higher by increasing the culture volume of respective bacterial cultures, from 10% to 25% (v/v), for 10 days of treatment at room temperature. The Pearson correlation between treatment duration and % degradation of chlorpyrifos were 0.953 and 0.988 with bacterial mono- and mixed-cultures, respectively. Source

Gohel S.D.,Saurashtra University | Singh S.P.,Saurashtra University
International Journal of Biological Macromolecules

An alkaline serine protease from a newly isolated salt-tolerant alkaliphilic actinomycetes, Brachystreptospora xinjiangensis OM-6 was purified with 35- and 26-fold purification and 47% and 22% yield employing two steps and one step methods, respectively. The enzyme was quite stable at 80°C in 30% Na-glutamate with the deactivation rate constant (Kd) 8.66 and half life (t1/2) 80.04min. The activation energies (E), enthalpy (ΔH*), entropy (ΔS*) and change in free energy (ΔG*) for the protease deactivation were calculated in the presence of 30% Na-glutamate and correlated with the enzyme stability. The thermodynamic analysis corresponded the trends of the enzyme stability and inactivation. The enzyme retained high activity and significant stability at higher salt, temperature, range of pH and metal ions. The enzyme was extremely resistant against urea denaturation, oxidizing and reducing agents and surfactants, a finding which is rather unique and restricted to only few proteins. © 2013 Elsevier B.V. Source

Sheth B.P.,Saurashtra University | Thaker V.S.,Saurashtra University

Plants dwelling at the base of biological food chain are of fundamental significance in providing solutions to some of the most daunting ecological and environmental problems faced by our planet. The reductionist views of molecular biology provide only a partial understanding to the phenotypic knowledge of plants. Systems biology offers a comprehensive view of plant systems, by employing a holistic approach integrating the molecular data at various hierarchical levels. In this review, we discuss the basics of systems biology including the various 'omics' approaches and their integration, the modeling aspects and the tools needed for the plant systems research. A particular emphasis is given to the recent analytical advances, updated published examples of plant systems biology studies and the future trends. © 2014 Springer-Verlag Berlin Heidelberg. Source

Purohit M.K.,Saurashtra University | Singh S.P.,Saurashtra University
International Journal of Biological Macromolecules

Metagenomics has opened new horizon to unlock the biotechnological potential for novel enzymes. An alkaline protease gene was obtained from the total environmental DNA extracted from a saline habitat. After cloning and sequencing, it was identified that the protease gene related to uncultivable bacteria (HM219181). The protease was over expressed at 6. h of induction with optimum induction at 1. mM IPTG and 27 °C. The purified enzyme was characterized with respect to various factors; temperature, pH, NaCl and chemical denaturant. The sequence analysis indicated a hydrophobic tendency of the protein, while the predicted 3D structure indicated the enzyme as a serine protease. © 2012 Elsevier B.V. Source

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