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Singh N.,IMS Engineering College | Mishra N.,Saroj Institute of Technology and Management | Chaurasia S.,IMS Engineering College
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2014

The hot water extraction process was used to isolate the water soluble polysaccharides from raw (R-WSP) and sprouted (S-WSP) mung beans (Vigna radiata). The chemical characteristics of both the samples were analyzed by FT-IR spectrum. Further, several biochemical assays were carried out to evaluate the antioxidant activity of the samples. The extraction yield of R-WSP and S-WSP were 49.1 mg/g and 10.5 mg/g respectively. The presence of uronic acid was analyzed in both the samples. The raw mung bean polysaccharides (R-WSP) exhibited higher antioxidant ability than the sprouted mung polysaccharides (S-WSP). The results suggested that germination causes the change in polysaccharides content and the water soluble polysaccharides of raw mung beans can be further deduce to identify the principal component for its antioxidant potential. Source


Kumar P.,Jaipur National University | Shrivastava B.,Jaipur National University | Pandeya S.N.,Saroj Institute of Technology and Management | Stables J.P.,U.S. National Institutes of Health
Medicinal Chemistry Research | Year: 2012

A series of 2-[2-(substituted)hydrazinyl]-1,3-benzothiazole and 2-(1,3-benzothiazol-2-ylsulfanyl)-N′-(substituted)acetohydrazide were designed and synthesized keeping in view the structural requirement of pharmacophore and evaluated for anticonvulsant activity and neurotoxicity. The anticonvulsant activity of the titled compounds was assessed using the 6 Hz psychomotor seizure test. The most active compound of the series was 2-(1,3-benzothiazol-2-ylsulfanyl)-N′-[4-(4-bromophenoxy)benzylidene] acetohydrazide BT 15, which showed 75% protection (3/4, 1.0 h) and 50% protection (2/4, 0.5 h) at a dose of 100 mg/kg in mice. A computational study was carried out for calculation of pharmacophore pattern and prediction of pharmacokinetic properties. Titled compounds have also exhibited good binding properties with epilepsy molecular targets such as GABA (A) alpha-1, glutamate, GABA (A) delta receptors and Na/H exchanger, in Lamarckian genetic algorithmbased flexible docking studies. © Springer Science+Business Media, LLC 2011. Source


Kumar P.,Jaipur National University | Shrivastava B.,Jaipur National University | Pandeya S.N.,Saroj Institute of Technology and Management | Stables J.P.,U.S. National Institutes of Health
European Journal of Medicinal Chemistry | Year: 2011

Thirty new 2-(substituted)-3-{[substituted]amino}quinazolin-4(3H)-one were designed and synthesized keeping in view the structural requirement of pharmacophore and evaluated for anticonvulsant activity and neurotoxicity. The anticonvulsant activity of the titled compounds was assessed using the 6 Hz psychomotor seizure test. The most active compound of the series was 3-({(E)-[3-(4-chloro-3-methylphenoxy)phenyl]methylidene}amino) -2-phenylquinazolin-4(3H)-one PhQZ 7, which showed 100% protection (4/4, 0.5 h) and 75% protection (3/4, 0.25 h) at a dose of 100 mg/kg in mice. A computational study was carried out for calculation of pharmacophore pattern and prediction of pharmacokinetic properties. Titled compounds have also exhibited good binding properties with epilepsy molecular targets such as glutamate, GABA (A) delta and GABA (A) alpha-1 receptors, in Lamarckian genetic algorithm based flexible docking studies. © 2011 Elsevier Masson SAS. All rights reserved. Source


Sonar P.K.,Babu Banarasi Das Northern India Institute of Technology | Verma A.,Saroj Institute of Technology and Management | Saraf S.K.,Babu Banarasi Das Northern India Institute of Technology
Oriental Pharmacy and Experimental Medicine | Year: 2013

In the present study, an attempt was made to evaluate the extract of the leaves of Rhododendron arboreum for immunomodulatory activity and related hepatotoxicity. Alcoholic extract of the leaves of R. arboreum was prepared by hot percolation method. Levamisole was used as the standard drug. The parameters like, humoral immune response, cell mediated immune response and total leukocyte counts (TLC) were assessed in the antigenically challenged mice with sheep RBC (SRBC). Liver function tests like total bilirubin, SGPT and SGOT were also evaluated. The, orally administered alcoholic extract of the leaves showed a significant suppression of the immune responses, in a dose dependent manner. The extract at the maximum dose (100 mg/kg) was found to possess higher immunosuppressant effect in comparison with control and levamisole (p < 0. 001). No significant difference was observed in the weights of liver and spleen of extract-treated mice in comparison with levamisole and vehicle treated mice. Liver function tests of extract-treated mice were also statistically insignificant in comparison with levamisole and vehicle-treated mice. Thus, it can be concluded that the alcoholic extract of R. arboreum is an effective and safe immunosuppressive agent. © 2013 Institute of Oriental Medicine, Kyung Hee University. Source


Zeashan H.,National Botanical Research Institute | Amresh G.,National Botanical Research Institute | Singh S.,Saroj Institute of Technology and Management | Rao C.V.,National Botanical Research Institute
Pharmaceutical Biology | Year: 2010

The current study is an effort to identify the hepatoprotective activity of the 50% ethanol extract of the whole plant of Amaranthus spinosus Linn. (Amaranthaceae) against d-galactosamine/lipopolysaccharide (d-GalN/LPS)-induced liver injury in rats. d-GalN/LPS (300mg/kg body weight/30 g/kg body weight)-induced hepatic damage was manifested by a significant (p <0.05) increase in the activities of marker enzymes (aspartate transaminase, alanine transaminase, alkaline phosphatase, lactate dehydrogenase and gamma glutamyl transferase) and bilirubin level in serum while phospholipids significantly decreased. All other parameters, i.e. cholesterol, triglycerides and free fatty acids were increased significantly in both serum and liver compared to the control group. Pretreatment of rats with A. spinosus extract (400mg/kg) significantly (p <0.05) reversed these altered parameters to normal compared to the intoxicated group. The biochemical observations were supplemented by histopathological examination of liver sections. There were no significant changes in the activities of marker enzymes, bilirubin level and lipids in the rats treated with A. spinosus extract alone. Results of this study revealed that A. spinosus extract could afford a significant protection against d-GalN/LPS-induced hepatocellular injury. © 2010 Informa Healthcare USA, Inc. Source

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