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Bisht G.S.,Sardar Bhagwan Singh Post Graduate Institute of Biomedical science and Research Balawala | Bharti A.,Sardar Bhagwan Singh Post Graduate Institute of Biomedical science and Research Balawala | Kumar V.,Sardar Bhagwan Singh Post Graduate Institute of Biomedical science and Research Balawala | Gusain O.,Hemwati Nandan Bahuguna Garhwal University
Proceedings of the National Academy of Sciences India Section B - Biological Sciences | Year: 2013

An actinobacterium strain designated as IN2-10 was isolated from Garhwal region. IN2-10 was subjected to polyphasic characterization. The organism produced white to cream colored aerial mycelium with brown diffusible pigment. This isolate showed growth at 11 % NaCl, pH 4-10, and a temperature range of 15-37 C. The isolate IN2-10 was found to belong to the genus Streptomyces and it was closely related to Streptomyces violascens (98.57 %, 16S rDNA). Of the various media tested, the high yield of active metabolite for isolate was observed in glucose soyabean meal medium at pH 10, with salt concentration of 3 % (w/v) and at a temperature of 37 C. The broad spectrum antifungal activity of metabolite was observed against yeasts (Candida albicans, C. parapsilosis, and C. tropicalis) as well as molds (Microsporum gypseum, M. canis, Trichophtyton rubrum, Aspergillus flavus, A. fumigatus, and A. niger). The active metabolite produced by the strain was partially characterized and was found to belong to the heptaene group of polyene class of antifungal agents. In view of the decline in the discovery of new lead compounds in recent years, further investigations would require to lead to some useful products. © 2012 The National Academy of Sciences, India.


Kumar V.,Sardar Bhagwan Singh Post Graduate Institute of Biomedical science and Research Balawala | Gusain O.,Hemwati Nandan Bahuguna Garhwal University | Thakur R.L.,Sardar Bhagwan Singh Post Graduate Institute of Biomedical science and Research Balawala | Bisht G.S.,Sardar Bhagwan Singh Post Graduate Institute of Biomedical science and Research Balawala
Proceedings of the National Academy of Sciences India Section B - Biological Sciences | Year: 2013

A halotolerant alkaliphilic actinomycetes strain EWC 7(2) was isolated from earthworm castings located in Dehradun, India. It showed the morphological, chemotaxonomic and physiological characteristics of the genus Streptomyces. On the basis of 16S rDNA sequence analysis and physiological tests, this isolate was found to be most closely related with Streptomyces ghanaensis. The optimum metabolite production was recorded in Glucose soyabean meal medium which is active against both Gram-positive and Gram-negative bacteria including drug resistant bacterial pathogens. The antibacterial activity started in the beginning of the exponential phase of growth (2 days) and reached maximum at 3-8 days while the maximum dry mycelial weight was obtained by 3-5 days of fermentation. The best growth and metabolite production was observed at a salt concentration of 4 % (w/v) and above pH 8. The antibiotic was extracted with HP Dianion resin eluted with methanol and detected by bioautography on silica gel plates using Micrococcus luteus MTCC106 as test organism. The results of bioautography revealed the presence of single active compound. The antibiotic compound was further purified by semi-prep HPLC. The spectroscopic studies such as UV-Vis, infrared, and mass of the compound showed that the molecule may be different from other known antibiotics produced by most closely related species of Streptomyces EWC 7(2). The results obtained from this study are promising however; further studies are required to elucidate the structure of the active compound. © 2012 The National Academy of Sciences, India.

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