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Srivastava N.,Sardar Bhagwan Singh Pg Institute Of Biomedical Science And Research Balawala | Sharma V.,Sardar Bhagwan Singh Pg Institute Of Biomedical Science And Research Balawala | Kamal B.,Sardar Bhagwan Singh Pg Institute Of Biomedical Science And Research Balawala | Dobriyal A.K.,Hemwati Nandan Bahuguna Garhwal University | Jadon V.S.,Sardar Bhagwan Singh Pg Institute Of Biomedical Science And Research Balawala
Biotechnology | Year: 2011

The present review is the pragmatic approach to ensue the findings on almost every aspect of very important herbal genus Aconitum, as a number of species belonging to this genus are renowned for their medicinal benefits in Ayurvedic, Unani, Chinese and Tibetan system of medicine. A lot of important attribute have been assigned to the species of this genus. Numerous works has been done on its various aspects from cultivation, conservation, phytochemical and pharmacological analysis to molecular characterization. The important species of this genus which have been explored so far include A. heterophyllum, A. carmichaelii, A. balfourii, A. napettus, A. anthora, A. laeve and A kusnezoffii. © 2011 Asian Network for Scientific Information. Source


Srivastava N.,Sardar Bhagwan Singh Pg Institute Of Biomedical Science And Research Balawala | Sharma V.,Sardar Bhagwan Singh Pg Institute Of Biomedical Science And Research Balawala | Dobriyal A.K.,Hemwati Nandan Bahuguna Garhwal University | Kamal B.,Sardar Bhagwan Singh Pg Institute Of Biomedical Science And Research Balawala | And 2 more authors.
Biotechnology | Year: 2011

In the present study in-vitro seed germination protocol using various pre-sowing treatments for Aconitum heterophyttum Wall intended for its ex-situ conservation was optimized. Temperature requirement for seed germination in this species was optimized under controlled conditions which revealed that low temperature of 15°C (p Source


Srivastava N.,Sardar Bhagwan Singh Pg Institute Of Biomedical Science And Research Balawala | Sharma V.,Sardar Bhagwan Singh Pg Institute Of Biomedical Science And Research Balawala | Saraf K.,Sardar Bhagwan Singh Pg Institute Of Biomedical Science And Research Balawala | Dobriya A.K.,Hnbgarhwal University | And 2 more authors.
Indian Journal of Natural Products and Resources | Year: 2011

Antimicrobial activity of extracts of aerial parts of the plant samples of Aconitum heterophyllum Wall. ex Royle was evaluated against different bacterial and fungal strains. It was observed that methanolic extract (50 mg/well) was the most potent extract which showed significant inhibition of the growth of Gram positive bacteria, Staphylococcus aureus and Bacillus subtilis. Antifungal activity was shown by two extracts against Candida albicans and Aspergillus flavus. Methanolic extract showed considerable antifungal activity while feeble antifungal activity was obtained with ethyl acetate extract even at higher concentrations. Source


Srivastava N.,Sardar Bhagwan Singh Pg Institute Of Biomedical Science And Research Balawala | Sharma V.,Sardar Bhagwan Singh Pg Institute Of Biomedical Science And Research Balawala | Kamal B.,Sardar Bhagwan Singh Pg Institute Of Biomedical Science And Research Balawala | Dobriyal A.K.,Central University of Costa Rica | Jadon V.S.,Sardar Bhagwan Singh Pg Institute Of Biomedical Science And Research Balawala
Journal of Plant Sciences | Year: 2010

The present study is aimed to isolate DNA from three types of tissues of Aconitum heterophyllum Wall using previously reported methods after modifications. Aconitum heterophyllum Wall is a vital and critically endangered herb, which needs to have its fingerprinting data for its shrewd use. Use of DNA markers with the techniques like Polymerase Chain Reaction (PCR) and molecular cloning can provide every minute detail about the subject sample. Good quality DNA is always a prerequisite in all molecular biology experiments especially PCR. As this plant contains high amounts of secondary metabolites that interfere with DNA isolation. The two methodologies described in the present study yielded good-quality, high-molecular weight DNA that is free of contaminants and colored pigments and is suitable for PCR amplification. © 2010 Academic Journals Inc. Source

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