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Belyanina S.I.,Saratov State Medical University
Russian Journal of Genetics | Year: 2012

The data on karyopool analysis of Chironomus plumosus from Novozybkov raion of Bryansk oblast, radioactively contaminated as a result of the Chernobyl accident, are presented. In the karyopool of this population, four types of heterozygous inversions (A1. 2, B1. 2, C1. 2, D1. 2) and a structurally small rearrangement in arm D, which is thought to be also associated with inversion, were identified. For inversions A1. 2, C1. 2, and structurally small rearrangement in arm D the cases of somatic mosaicism were described. The mean number of inversions per individual constituted 0. 78. The number of genotypic combinations was 13. In 5% of the individuals chromosome B was identified, while 15. 6% were polyploid (3n). In 1. 8% of triploids somatic mosaicism for the level of polytene chromosomes was observed. Most of the larvae were characterized by partial asynapsis of the homologs in different regions of chromosome III. Specific features of the Chironomus plumosus karyopool from Novozybkov are thought to be associated with the habitation of a number of generations of this population on radioactively polluted territory. © 2012 Pleiades Publishing, Ltd.

Herrschaft H.,University of Cologne | Nacu A.,Moldova State University | Sholomov I.,Saratov State Medical University | Hoerr R.,Dr. Willmar Schwabe GmbH and Co. KG Pharmaceuticals | Schlaefke S.,Dr. Willmar Schwabe GmbH and Co. KG Pharmaceuticals
Journal of Psychiatric Research | Year: 2012

A multi-centre, double-blind, randomised, placebo-controlled, 24-week trial with 410 outpatients was conducted to demonstrate efficacy and safety of a 240 mg once-daily formulation of Ginkgo biloba extract EGb 761 ® in patients with mild to moderate dementia (Alzheimer's disease or vascular dementia) associated with neuropsychiatric symptoms. Patients scored 9 to 23 on the SKT cognitive battery, at least 6 on the Neuropsychiatric Inventory (NPI), with at least one of four key items rated at least 4. Primary outcomes were the changes from baseline to week 24 in the SKT and NPI total scores. The ADCS Clinical Global Impression of Change (ADCS-CGIC), Verbal Fluency Test, Activities of Daily Living International Scale (ADL-IS), DEMQOL-Proxy quality-of-life scale and 11-point box scales for tinnitus and dizziness were secondary outcome measures. Patients treated with EGb 761 ® (n = 200) improved by 2.2 ± 3.5 points (mean ± sd) on the SKT total score, whereas those receiving placebo (n = 202) changed only slightly by 0.3 ± 3.7 points. The NPI composite score improved by 4.6 ± 7.1 in the EGb 761 ®-treated group and by 2.1 ± 6.5 in the placebo group. Both drug-placebo comparisons were significant at p < 0.001. Patients treated with EGb 761 ® also showed a more favourable course in most of the secondary efficacy variables. In conclusion, treatment with EGb 761 ® at a once-daily dose of 240 mg was safe and resulted in a significant and clinically relevant improvement in cognition, psychopathology, functional measures and quality of life of patients and caregivers. © 2012 Elsevier Ltd.

Doubrovskii V.A.,Saratov State Medical University | Yanina I.Y.,Chernyshevsky Saratov State University | Tuchin V.V.,Russian Academy of Sciences
Biophysics | Year: 2012

Changes in the light transmission coefficient of human adipose cells as a result of photodynamic action have been detected and investigated. Based on the statistical processing of digital micrographs of thin tissue slices, the kinetics of processes occurring in the adipose tissue has been quantitatively estimated. It has been suggested that the effects observed are brought about through the lipolysis of adipose cells with the retention of their structure. © 2012 Pleiades Publishing, Ltd.

Cytogenetic analysis was performed on samples of Chironomus plumosus L. (Diptera, Chironomidae) taken from waterbodies of various types in Bryansk region (Russia) and Gomel region (Belarus). Karyotypes of specimens taken from stream pools of the Volga were used as reference samples. The populations of Bryansk and Gomel regions (except for a population of Lake Strativa in Starodubskii district, Bryansk region) exhibit broad structural variation, including somatic mosaicism for morphotypes of the salivary gland chromosome set, decondensation of telomeric sites, and the presence of small structural changes, as opposed to populations of Saratov region. As compared with Saratov and Bryansk regions, the Balbiani ring in the B-arm of chromosome I is repressed in populations of Gomel region. It is concluded that the chromosome set of Ch. plumosus in a range of waterbodies of Bryansk and Gomel regions is unstable. © 2015, Pleiades Publishing, Inc.

The karyopools of the phytophilous chiromomid species of Glyptotendipes glaucus (Mg.) were studied. Chironomids originated from a number of reservoirs located in the Novozybkovsky raion of the Bryansk region, which was affected by the Chernobyl radioactive release, and two reservoirs located in the Saratov region. Differences in the inversion spectrum and frequencies, both among Bryansk and between Bryansk and Saratov populations, were found. There were no new inversions in the Novozybkovsky populations; however, structurally small rearrangements in long chromosomes were noted. Typical abnormalities included mosaicism of the chromosome morphotypes in cells of the same saline gland, which was especially distinctive in the larvae from the forbidden zone; decondensation of the telomere regions of chromosomes; and mosaic asynapsis of the chromosome IV homologs (up to complete disjunction). Also, several larvae were polyploids. Other species of Glyptotendipes inhabiting the Novozybkovsky reservoirs were represented by the single species of G. paripes (near the Korchy settlement). The karyotypes of its several larvae were represented by an unorganized chromosomal substance. The other Glyptotendipes species seem to have lower adaptive abilities under the conditions in question and were eliminated from precatastrophe biotopes, while G. glaucus succeeded in adaptating to the new environment. © 2014, Pleiades Publishing, Inc.

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