Saratov Research Institute of Traumatology and Orthopedics

Saratov, Russia

Saratov Research Institute of Traumatology and Orthopedics

Saratov, Russia
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Pavlenko N.N.,Saratov Research Institute of Traumatology and Orthopedics | Korshunov G.V.,Saratov Research Institute of Traumatology and Orthopedics | Matveeva O.V.,Saratov Research Institute of Traumatology and Orthopedics | Kesov L.A.,Saratov Research Institute of Traumatology and Orthopedics | And 3 more authors.
Voprosy Onkologii | Year: 2016

The article contains diagnostic errors analysis of 439 patients with giant-cell tumor (176), bone cyst (158) and osteosarcoma (105) aged 4-75 for the period of 1990-2015. Morphological investigation of surgical material taken from the pathological focus showed that diagnostic errors in clinical and X-ray examinations comprised up to 40.6% in giant-cell cases, 37.2% in bone cyst cases and 34.2% (p<0.05) in osteosarcoma cases.


Shul'ga A.E.,Saratov Research Institute of Traumatology and Orthopedics | Norkin I.A.,Saratov Research Institute of Traumatology and Orthopedics | Ninel' V.G.,Saratov Research Institute of Traumatology and Orthopedics | Puchin'yan D.M.,Saratov Research Institute of Traumatology and Orthopedics | And 4 more authors.
Neuroscience and Behavioral Physiology | Year: 2015

The pathogenesis of traumatic spinal cord disease involves two mechanisms causing injury to its neuronal apparatus: primary (necrosis) and secondary (apoptosis). This study assesses the involvement of a number of endogenous factors in the development of apoptosis in spinal cord and peripheral nerve trunk trauma; the role of these factors thus far remains unclear. © 2015, Springer Science+Business Media New York.


Al-Bayati B.M.,Chernyshevsky Saratov State University | Nechaeva O.V.,Saratov State Medical University | Ulyanov V.Y.,Saratov Research Institute of Traumatology and Orthopedics | Glinskaya E.V.,Chernyshevsky Saratov State University | And 5 more authors.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE | Year: 2017

The current study was designed to investigate the dynamics of microbial biofilm-formation in standard and clinical strains of uropathogenic Escherichia coli (UPEC), using spectrophotometric and electron microscopic methods. For achieving this goal, three E. coli strains were used (one standard and two clinical). These isolates differ in the existence of fimH virulence gene. The process involved the using of electron microscopy and immunological microtitration plates under in vitro conditions for 96 hours. We found that standard and clinical strains of E. coli were capable of forming a microbial biofilm in vitro, with a higher intensity related to the clinical strain E. coli 245. The using of scanning electron microscopy confirmed the obtained results. The combined use of different optical methods gives a complete characterization of film-forming ability of pathogenic microorganisms. © COPYRIGHT SPIE.


Lyasnikova A.V.,Saratov State Technical University | Markelova O.A.,Saratov State Technical University | Dudareva O.A.,Saratov State Technical University | Lyasnikov V.N.,Saratov State Technical University | And 2 more authors.
Powder Metallurgy and Metal Ceramics | Year: 2016

The physicochemical and biomedical properties of plasma-sprayed biocomposite coatings based silver- and copper-substituted hydroxyapatite powders on a titanium substrate are comprehensively studied and compared. Infrared spectroscopy shows that powder samples of all types correspond mainly to synthetic hydroxyapatite. Scanning electron microscopy confirmed the presence of metal-substituted hydroxyapatite nanoparticles in all coatings. The coatings are characterized by marked hydrophilic properties, the greatest ones being shown by silver-substituted hydroxyapatite coatings. The coatings produced from copper-substituted hydroxyapatites exhibit greater biological activity and the highest adhesion properties, but pronounced antimicrobial properties are possessed only by silver-substituted hydroxyapatite coatings. © 2016 Springer Science+Business Media New York


Babushkina I.V.,Saratov Research Institute of Traumatology and Orthopedics
World Journal of Medical Sciences | Year: 2013

The effect of topical application of copper and zinc nanoparticle suspensions on the dynamics of bacterial contamination of the experimental purulent wound was studied. A model of purulent wound infected with polyantibiotic-resistant clinical strains of Staphylococcus aureus isolated from orthopedic trauma patients was obtained in 30 white male rats. The use of copper nanoparticle suspension ensured rapid elimination of the infectious agent contaminating the wound; zinc nanoparticles had a weaker antibacterial effect: the St. aureus culture was disseminated until day 12 (until day 20 in the control group). The quantitative study of microbial contamination of 1 g of wound tissue demonstrated that it decreased to 102CFU/g in the experimental animals treated with copper nanoparticles. The use of zinc nanoparticles to treat the wound resulted in a lesser decrease in the count of microbial bodies: the CFU/g value decreased to 105 CFU/ml on day 5, which also reliably differed from the control group, where an increase in the degree of bacterial contamination of the wound was observed at this time. The studies revealed the high antibacterial activity of copper nanoparticle suspension against clinical polyantibiotic-resistant strains of St. aureus under conditions of experimental purulent would. © IDOSI Publications, 2013.


Babushkina I.V.,Saratov Research Institute of Traumatology and Orthopedics | Mamontova I.A.,Saratov Research Institute of Traumatology and Orthopedics | Gladkova E.V.,Saratov Research Institute of Traumatology and Orthopedics
Bulletin of Experimental Biology and Medicine | Year: 2015

Bacterial contamination of experimental purulent wound in rats treated by local applications of suspension of copper and zinc nanoparticles and a combined drug based on chitosan and copper and zinc nanoparticles was evaluated. Applications of copper nanoparticle suspension and combined drug with copper and zinc nanoparticles and chitosan led to rapid elimination of the bacterial contaminant. Antibacterial activity of zinc nanoparticles was less pronounced, but the effect also differed significantly from the reference group. © 2015, Springer Science+Business Media New York.


Belova S.V.,Saratov Research Institute of Traumatology and Orthopedics | Norkin I.A.,Saratov Research Institute of Traumatology and Orthopedics | Puchin'yan O.M.,Saratov Research Institute of Traumatology and Orthopedics
Eksperimental'naya i Klinicheskaya Farmakologiya | Year: 2015

The influence of administration of the antioxidant complexes consisting of nonenzymatic antioxidants (alpha-tocopherol acetate preparation) and enzymatic antioxidants (ceruloplasmin) has been studied in rabbits with experimental arthritis. The introduction of alpha-tocopherol acetate (at a daily dose of 4 mg) improved metabolic processes in the organism (decreased in the rate of erythrocyte precipitation, total leukocytes and their stub and segmental forms; increased in erythrocyte count; reduced the glycosaminoglycan content as determined from uronic acid and hexose level; decreased ceruloplasmin activity and malonic dialdehyde level ion blood serum, all at p < 0.05), thus favoring reduction in the total activity of the inflammatory process as judged from hematological and biochemical data. Intra-articular introduction of ceruloplasmin (1.5 mg/kg, once per week) positively influenced the state of joint structures in damaged knee joints of the animals: decreased the activity of ceruloplasmin (from 5.28 ± 0.06 to 3.94 ± 0.01 AU), and malonic dialdehyde level (0.18 ± 0.02 to 0.08 ± 0.01 μM) in the articular fluid (all at p < 0.05). These effects are probably related to the elimination of inefficiency of the antioxidant system in the synovial medium, thus preventing inflammatory destruction of articular tissues, hindering the development of pannus, and assisting the activation of reparative regeneration of connective tissue structures.


Gladkova E.V.,Saratov Research Institute of Traumatology and Orthopedics | Babushkina I.V.,Saratov Research Institute of Traumatology and Orthopedics | Norkin I.A.,Saratov Research Institute of Traumatology and Orthopedics | Mamonova I.A.,Saratov Research Institute of Traumatology and Orthopedics | And 2 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2016

We developed wound coating based on natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol). Experiments on albino male rats demonstrated its regeneratory, antioxidant, and antibacterial effects on wounds involving all layers of the skin. Due to chemical composition and buffer component, the biodegraded wound coating optimizes all phases of the wound process, accelerates by 22-28% the reparative regeneration, and leads to anatomic and functional restoration of injured sites. High absorption capacity recommends its use in the treatment of wounds with profuse exudation. © 2016 Springer Science+Business Media New York


PubMed | Saratov Research Institute of Traumatology and Orthopedics
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2015

Bacterial contamination of experimental purulent wound in rats treated by local applications of suspension of copper and zinc nanoparticles and a combined drug based on chitosan and copper and zinc nanoparticles was evaluated. Applications of copper nanoparticle suspension and combined drug with copper and zinc nanoparticles and chitosan led to rapid elimination of the bacterial contaminant. Antibacterial activity of zinc nanoparticles was less pronounced, but the effect also differed significantly from the reference group.


PubMed | Saratov Research Institute of Traumatology and Orthopedics
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

We developed wound coating based on natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol). Experiments on albino male rats demonstrated its regeneratory, antioxidant, and antibacterial effects on wounds involving all layers of the skin. Due to chemical composition and buffer component, the biodegraded wound coating optimizes all phases of the wound process, accelerates by 22-28% the reparative regeneration, and leads to anatomic and functional restoration of injured sites. High absorption capacity recommends its use in the treatment of wounds with profuse exudation.

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