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Babushkina I.V.,Saratov Research Institute of Traumatology and Orthopedics
World Journal of Medical Sciences | Year: 2013

The effect of topical application of copper and zinc nanoparticle suspensions on the dynamics of bacterial contamination of the experimental purulent wound was studied. A model of purulent wound infected with polyantibiotic-resistant clinical strains of Staphylococcus aureus isolated from orthopedic trauma patients was obtained in 30 white male rats. The use of copper nanoparticle suspension ensured rapid elimination of the infectious agent contaminating the wound; zinc nanoparticles had a weaker antibacterial effect: the St. aureus culture was disseminated until day 12 (until day 20 in the control group). The quantitative study of microbial contamination of 1 g of wound tissue demonstrated that it decreased to 102CFU/g in the experimental animals treated with copper nanoparticles. The use of zinc nanoparticles to treat the wound resulted in a lesser decrease in the count of microbial bodies: the CFU/g value decreased to 105 CFU/ml on day 5, which also reliably differed from the control group, where an increase in the degree of bacterial contamination of the wound was observed at this time. The studies revealed the high antibacterial activity of copper nanoparticle suspension against clinical polyantibiotic-resistant strains of St. aureus under conditions of experimental purulent would. © IDOSI Publications, 2013. Source


Babushkina I.V.,Saratov Research Institute of Traumatology and Orthopedics | Mamontova I.A.,Saratov Research Institute of Traumatology and Orthopedics | Gladkova E.V.,Saratov Research Institute of Traumatology and Orthopedics
Bulletin of Experimental Biology and Medicine | Year: 2015

Bacterial contamination of experimental purulent wound in rats treated by local applications of suspension of copper and zinc nanoparticles and a combined drug based on chitosan and copper and zinc nanoparticles was evaluated. Applications of copper nanoparticle suspension and combined drug with copper and zinc nanoparticles and chitosan led to rapid elimination of the bacterial contaminant. Antibacterial activity of zinc nanoparticles was less pronounced, but the effect also differed significantly from the reference group. © 2015, Springer Science+Business Media New York. Source


Belova S.V.,Saratov Research Institute of Traumatology and Orthopedics | Norkin I.A.,Saratov Research Institute of Traumatology and Orthopedics | Puchin'yan O.M.,Saratov Research Institute of Traumatology and Orthopedics
Eksperimental'naya i Klinicheskaya Farmakologiya | Year: 2015

The influence of administration of the antioxidant complexes consisting of nonenzymatic antioxidants (alpha-tocopherol acetate preparation) and enzymatic antioxidants (ceruloplasmin) has been studied in rabbits with experimental arthritis. The introduction of alpha-tocopherol acetate (at a daily dose of 4 mg) improved metabolic processes in the organism (decreased in the rate of erythrocyte precipitation, total leukocytes and their stub and segmental forms; increased in erythrocyte count; reduced the glycosaminoglycan content as determined from uronic acid and hexose level; decreased ceruloplasmin activity and malonic dialdehyde level ion blood serum, all at p < 0.05), thus favoring reduction in the total activity of the inflammatory process as judged from hematological and biochemical data. Intra-articular introduction of ceruloplasmin (1.5 mg/kg, once per week) positively influenced the state of joint structures in damaged knee joints of the animals: decreased the activity of ceruloplasmin (from 5.28 ± 0.06 to 3.94 ± 0.01 AU), and malonic dialdehyde level (0.18 ± 0.02 to 0.08 ± 0.01 μM) in the articular fluid (all at p < 0.05). These effects are probably related to the elimination of inefficiency of the antioxidant system in the synovial medium, thus preventing inflammatory destruction of articular tissues, hindering the development of pannus, and assisting the activation of reparative regeneration of connective tissue structures. Source


Gladkova E.V.,Saratov Research Institute of Traumatology and Orthopedics | Babushkina I.V.,Saratov Research Institute of Traumatology and Orthopedics | Norkin I.A.,Saratov Research Institute of Traumatology and Orthopedics | Mamonova I.A.,Saratov Research Institute of Traumatology and Orthopedics | And 2 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2016

We developed wound coating based on natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol). Experiments on albino male rats demonstrated its regeneratory, antioxidant, and antibacterial effects on wounds involving all layers of the skin. Due to chemical composition and buffer component, the biodegraded wound coating optimizes all phases of the wound process, accelerates by 22-28% the reparative regeneration, and leads to anatomic and functional restoration of injured sites. High absorption capacity recommends its use in the treatment of wounds with profuse exudation. © 2016 Springer Science+Business Media New York Source


Ivanov A.N.,Saratov Research Institute of Traumatology and Orthopedics | Kozadaev M.N.,Saratov Research Institute of Traumatology and Orthopedics | Bogomolova N.V.,Saratov Research Institute of Traumatology and Orthopedics | Matveeva O.V.,Saratov Research Institute of Traumatology and Orthopedics | And 4 more authors.
Cell and Tissue Biology | Year: 2015

Biocompatibility of the matrix is one of the major requirements for a functional scaffold. In this study, we carried out an assessment of biocompatibility of a complex matrix composed of polycaprolactone and hydroxyapatite under in vivo conditions by examining the dynamics of cell distribution in the original scaffold, as well as the reactions to the implant by the surrounding tissues. As result of these studies, we found that, upon subcutaneous implantation of polycaprolactone and hydroxyapatite matrix in white rats, reactive changes in the perifocal zone were completely relieved by the 21st day of the experiment. The matrix was actively populated by cells of connective tissue in the period from the 7th to 21st day of the experiment. In turn, we detected intense vascularization of the scaffold starting at the 14th day after implantation. The obtained data indicate a high degree of biocompatibility of a scaffold constructed on the basis of polycaprolactone and hydroxyapatite. © 2015, Pleiades Publishing, Ltd. Source

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