San Diego, CA, United States
San Diego, CA, United States

Sapphire Energy is a San Diego-based American energy company that produces crude oil made from algae. Wikipedia.


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Patent
Sapphire Energy | Date: 2013-11-25

The present application includes methods to harvest a non-vascular photosynthetic organism (NVPO) such as microalgae from an aqueous culture comprising brackish, non-brackish, marine, sea or saline water using polymer flocculants. The methods are suitable for harvesting NVPO from aqueous culture with total dissovled solids (TDS) of at least 1500 mg/L. Methods are also provided to harvest a NVPO using flocculation with or without a Dissolved Air Flotation (DAF) process. Methods are further provided to flocculate and harvest a NVPO directly in a pond. The present application further provides NVPO-containing intermediates, compositions, or products produced by the methods provided herein.


Patent
Sapphire Energy | Date: 2014-11-04

Provided herein are compositions and methods for engineering salt tolerance and producing products by photosynthetic organisms. The photosynthetic organisms can be genetically modified to be salt tolerant as compared to an unmodified organism and to produce useful products. The methods and compositions of the disclosure are useful in many therapeutic and industrial applications.


Patent
Sapphire Energy and Scripps Research Institute | Date: 2016-01-28

The present disclosure relates to methods of expressing therapeutic proteins in photosynthetic organisms and the therapeutic proteins produced by the methods. The therapeutic proteins include high-mobility group box 1 (HMGB1) protein, fibronectin domain (10) (10FN3), fibronectin domain (14) (14FN3), interferon beta (IFN), proinsulin and vascular endothelial growth factor (VEGF). The photosynthetic organisms include prokaryotes such as cyanobacteria and eukaryotes such as alga and plants. Transformation of eukaryotes is preferably the plastid genome, more preferably the chloroplast genome.


Patent
Sapphire Energy | Date: 2016-04-07

Disclosed herein are transformed non-vascular photosynthetic organisms that are salt tolerant, nucleotides and vectors useful in conducting such transformations, and transformed strains produced by such transformations.


Patent
Sapphire Energy | Date: 2014-07-11

Algae transformed with one or more polynucleotides encoding proteins that confer herbicide resistance are provided. The algae can be grown in small or large scale cultures that include one or more herbicides for the production and isolation of various products.


Patent
Sapphire Energy | Date: 2014-11-05

Disclosed herein are methods and processes for the recovery of oleaginous compounds from biomass and in particular biomass comprises photosynthetic microorganisms. Also disclosed are oleaginous compounds obtained using the disclosed methods.


Patent
Sapphire Energy | Date: 2015-01-23

The present invention provides compositions and methods for producing flocculation moieties in photosynthetic organisms. The photosynthetic organisms are genetically modified to effect production, secretion, or both, of the flocculation moieties. Also provided are methods of flocculating organisms.


Patent
Sapphire Energy | Date: 2013-11-20

The present invention provides compositions and methods for producing flocculation moieties in photosynthetic organisms. The photosynthetic organisms are genetically modified to effect production, secretion, or both, of the flocculation moieties. Also provided are methods of flocculating organisms.


Patent
Sapphire Energy | Date: 2014-08-28

Terpene synthases are enzymes that directly convert IPP & DMAPP to terpenes, such as fusicoccadiene. Described herein are methods and compositions for the production of terpenes and terpenoids for use as fuel molecules or other useful components. Genetically engineered enzymes capable of producing terpenes and terpenoids are also described.


Patent
Sapphire Energy and Scripps Research Institute | Date: 2014-08-13

The present invention provides compositions and methods for producing products, such as isoprenoids, which can be used for multiple purposes (e.g., fuel, fuel feedstocks, fragrances and insecticides), using photosynthetic organisms. One aspect of the present invention provides a vector comprising a nucleic acid encoding an enzyme that produces an isoprenoid with two phosphates and a promoter configured for expression of the nucleic acid in a chloroplast of a non-vascular, photosynthetic organism and does not comprise the entire genome of a chloroplast.

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