Presidente Prudente, Brazil
Presidente Prudente, Brazil

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Mariano E.,University of Sao Paulo | Leite J.M.,University of Sao Paulo | Vieira-Megda M.X.,State University of Montes Claros | Ciampitti I.A.,Kansas State University | And 4 more authors.
Crop Science | Year: 2016

Knowing the right fertilizer N source is a key management practice for improving nutrient use and productivity for sugarcane (Saccharum spp.). Therefore, this study evaluated the effect of fertilizer N sources on aboveground biomass (BM) and nutrient (N, P, K, Ca, Mg, and S) content for sugarcane ratoon. Two field trials under highly weathered tropical soils (Site 1, Typic Hapludox; Site 2, Typic Eutrustox) were conducted in the southeast region of Brazil. Five fertilizer N sources (organomineral fertilizer [OMF], ammonium chloride [AC], ammonium nitrate [AN], calcium ammonium nitrate [CAN], and urea) were applied on sugarcane at a rate of 100 kg N ha−1. A control treatment was also included in the randomized complete block design. At harvest, total BM varied from 24.4 Mg ha−1 (control and AC) at Site 1 to 44.2 Mg ha−1 (CAN) at Site 2, total K content varied from 185 kg ha−1 (AC) at Site 2 to 747 kg ha−1 (OMF) at Site 1, and total N content ranged from 92 kg ha−1 (AC) at Site 2 to 165 kg ha−1 (AN) at Site 1. Use of CAN and OMF resulted in comparable or superior BM and nutrient content relative to the other N fertilizers. In contrast, AC did not increase BM and nutrient content (except for N and Mg at Site 1), but also decreased by 33% total N content than control at Site 2. Use of more efficient fertilizer N sources (e.g., CAN and OMF) could increase productivity and nutrient use for sugarcane ratoon systems. © Crop Science Society of America.


Mariano E.,University of Sao Paulo | Vitti A.C.,Sao Paulo Agency of Agribusiness Technology | Otto R.,University of Sao Paulo | Trivelin P.C.O.,University of Sao Paulo
Sugar Tech | Year: 2011

The application of micronutrients on the sugarcane crop in Brazil is not a widespread practice, due to the few studies developed under Brazilian conditions. Nevertheless, the crop advance to lower fertility soils and the introduction of varieties with greater yield potential will change the nutrient management. In this context, two experiments were developed with boron (B) or zinc (Zn) application with the aim of evaluating yield, technological attributes and biometric indexes of sugarcane during the plant cane cycle and the residual effect of this fertilization on the first ratoon. The experiments were developed in Pirassununga, São Paulo, Brazil. In both trials, the doses of B (2 and 4 kg ha-1) and Zn (3 and 6 kg ha-1) were applied in top dressing. The crop was harvested around 13 months after the treatments application and, after the first harvest, all the plots received the same doses of N, P2O5 and K2O, to evaluating the residual effect on the first ratoon. The B application reduced the above ground biomass, TSS (tons of sugarcane stalks per hectare) and sugar yield in the plant cane. However, the Zn fertilization improved the stalk technological quality, as well as providing a residual effect, increasing the above ground biomass, TSS and stalk yield (dry matter). The dry matter accumulation showed three growth phases during the plant cane cycle, similar to the B and Zn accumulation. The fertilization with B, especially with high solubility sources, must be careful to avoid yield reduction. © 2011 Society for Sugar Research & Promotion.


da Silva A.,University of Sao Paulo | Rossetto R.,Sao Paulo Agency of Agribusiness Technology | Bombecini J.,University of Sao Paulo | Piemonte M.,Sao Paulo Agency of Agribusiness Technology | Muraoka T.,University of Sao Paulo
Journal of Plant Nutrition | Year: 2014

Vinasse is a liquid residue applied as a fertilizer in sugarcane crops. However, in areas far away from sugarcane mills the cost of the distribution can be very high due, the large volume of water. So, one solution is to concentrate the vinasse by the evaporation process to reduce transport costs. Considering that the nitrogen mineralization kinetics is not known in concentrated vinasse, the objective of this study was to evaluate the net and potential nitrogen mineralization in soil that received vinasse concentrated and not concentrated. The treatments with concentrated vinasse provided the highest values of net mineralization and total nitrogen, which presented significant correlation each other, the same way as the potential mineralization values had a positive correlation with the doses and the amounts of total nitrogen. The total nitrogen values can be used as an index of nitrogen availability in soils that received concentrated vinasse. © 2014 Copyright © Taylor & Francis Group, LLC.


Gomes V.,University of Sao Paulo | Baccili C.C.,University of Sao Paulo | Baldacim V.A.P.,University of Sao Paulo | Madureira K.M.,Federal University of Bahia | And 3 more authors.
American Journal of Animal and Veterinary Sciences | Year: 2014

Bovine colostrum contains immune factors, like leukocytes and cytokines that are absorbed by the intestinal mucosa of the neonate, however, not much is known about the function of some of these components in the active immune response of calves. The aim of this study was to determine the influence of colostrum intake and development of the innate immune response of calves from birth to two months of age, by evaluating the proportion of neutrophils CH138+ and monocytes CD14+, using flow citometry and measurement of proinflammatory cytokines IL-1 beta, IL-6, TNF-alpha and IFN-gamma, using comercial ELISA kits. According to the obtained results we can determine that colostrum intake had influenced the IFN-gamma concentration and the monocytes CD14+ and neutrophils CH138+ proportions in the circulation of calves and that these components seem to play a key role in the development of immune response of these animals, providing protection against pathogens, enhancing the phagocytic activity and antigen presentation by monocytes. © 2014 V. Gomes et al.


Allendorf S.D.,São Paulo State University | Albas A.,Sao Paulo Agency of Agribusiness Technology | Cipriano J.R.B.,São Paulo State University | Antunes J.M.A.P.,São Paulo State University | And 3 more authors.
Journal of Venomous Animals and Toxins Including Tropical Diseases | Year: 2011

Current knowledge on bat lyssavirus infections in their native hosts is limited and little is known about the virulence, virus dissemination and transmission among free-living insectivorous bats. The present study is a brief description of rabies virus (RABV) dissemination in tissues of a naturally infected pregnant southern yellow bat (Lasiurus ega) and its fetuses, obtained by reverse-transcriptase polymerase chain reaction (RT-PCR). The RT-PCR was positive in samples from the brain, salivary gland, tongue, lungs, heart, kidneys and liver. On the other hand, the placenta, three fetuses, spleen, intestine and brown fat tissue tested negative. This research demonstrated the absence of rabies virus in the fetuses, thus, in this specific case, the transplacentary transmission was not observed. © CEVAP 2011.


Curci V.C.M.,Sao Paulo Agency of Agribusiness Technology | Nogueira A.H.C.,Sao Paulo Agency of Agribusiness Technology | Nobrega F.L.C.,São Paulo State University | Araujo R.F.,São Paulo State University | And 3 more authors.
Journal of Venomous Animals and Toxins Including Tropical Diseases | Year: 2010

Types C and D strains of Clostridium botulinum are commonly related to avian and mammalian botulism. Although there are numerous vaccine recommendations, little research has been conducted to indicate the real effectiveness of vaccine timing or the ideal immunization protocol for young beef calves. Four commercially available vaccines, two bivalent (Clostridium botulinum types C and D; vaccines 1 and 2) and two polyvalent (all Clostridium spp. including Clostridium botulinum types C and D; vaccines 3 and 4), that are currently used in Brazilian herds, were tested in order to verify the maternal immune response. One hundred cows, divided into four vaccinated groups and one unvaccinated group, were given a two-dose subcutaneous immunization, at day zero, followed by a second dose given at 42 days post-vaccination, which corresponded to 40 days before birth. Serum samples (n = 75) were collected only from healthy neonatal calves at 0, 7, 45 and 90 days post-calving (DPC) and subjected to indirect ELISA using the purified C and D holotoxins as capture antigens. The serological profile showed that all vaccines were able to induce a satisfactory neonatal immune response to both holotoxins at 7 DPC. However, at 45 and 90 DPC, a significant reduction (p < 0.05) was observed in the antibody level against C and D holotoxins in all tested vaccines. Neonatal immunization in calves is compromised by significant levels of maternal antibodies so that the necessity of planning a calf vaccination program involves assessment of disease risks at the production site. Finally, our findings represent the first demonstration of maternal immunity transferred to neonatal beef calves, including immunity levels after vaccination against Clostridium botulinum toxoids C and D. © CEVAP 2010.


Camargo J.B.,São Paulo State University | Langoni H.,São Paulo State University | Troncarelli M.Z.,São Paulo State University | Machado J.G.,São Paulo State University | And 3 more authors.
Journal of Venomous Animals and Toxins Including Tropical Diseases | Year: 2010

Canine visceral leishmaniasis (CVL) is endemic in numerous Brazilian regions. The greatest difficulty in controlling the disease is the diagnostic limitation. In the present study, the most common tests employed for visceral leishmaniasis diagnosis were compared: immunofluorescence antibody test (IFAT), immunoenzymatic assay (ELISA), direct parasitological examination and polymerase chain reaction (PCR). Samples of lymph node aspirates and blood were collected from 100 dogs that lived in an endemic area (Bauru city, São Paulo state) and from 100 negative controls from a non-endemic area (Botucatu city, São Paulo state). Specificity of both IFAT and PCR was 100% whereas ELISA was 99%. Sensitivities were 97.77, 93.33 and 91.11% respectively for IFAT, ELISA and PCR. © CEVAP 2010.

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