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Rosa M.B.,Sao Leopoldo Mandic Institute and Research Center
The International journal of oral & maxillofacial implants | Year: 2013

The mental foramen is an important landmark during surgical procedures such as osseous grafting or the placement of dental implants. To avoid injuring the mental nerve, it is important both to carefully assess the location of the mental foramen and to determine whether an anterior loop of the mental nerve or the incisive canal lies mesial to it. The objective of this study was to quantify the ability of cone beam computed tomography (CBCT) to measure the length of the mental nerve loop, the length and diameter of the incisive nerve canals, and the incisive canal path. The study included 352 CBCT scans that had originally been used for preoperative planning of implant placement in the interforaminal region of the anterior mandible. For each scan, the length of the mental nerve loop and the length, diameter, and path of the incisive canal were determined. Mean values were compared between groups based on sex, right versus left side, and whether the patient was edentulous. The inferior alveolar nerve loop and incisive canal had a mean length of 2.40 ± 0.93 mm and 9.11 ± 3.00 mm, respectively. The mean incisive canal diameter was 1.48 ± 0.66 mm and showed a downward path in 51.3% of CBCT images and a linear or upward path in 38.29% and 10.41% of scans, respectively. CBCT provides an accurate means to identify critical anatomical features in the anterior mandible during preoperative surgical planning. Source


Soares A.B.,Sao Leopoldo Mandic Institute and Research Center | Altemani A.,University of Campinas | de Araujo V.C.,Sao Leopoldo Mandic Institute and Research Center
Journal of Oral Pathology and Medicine | Year: 2011

Background: Recurrent pleomorphic adenoma (RPA) is an uncommon and challenging disease. The aim of this study was to review the clinical information obtained by examining RPA patients, to determine its histomorphological features and to verify the expression of the Mcm-2 markers of cell proliferation and the expression of p-53 in pleomorphic adenoma (PA), RPA, and RPA with malignant transformation (TRPA). Methods: A total of 10 cases of PA and 29 cases of RPA were examined in detail for the presence of nodules and their histomorphological features. Cell proliferation and expression of p-53 were detected by the immunohistochemical technique using the antibodies against Mcm-2 and p-53. Results: Histopathologically, RPA is very similar to PA; the only difference found was that all the cases of RPA were multinodular. When comparing primary and recurrent tumor, no significant difference was found in terms of cell proliferation and the expression of p-53; however, in the RPA with areas of malignant transformation there was an increased expression of these proteins. Conclusion: This study showed that recurrences were multinodular, with nodules varying in numbers and sizes. No significant difference in histological features was found between RPA and PA. Moreover, the immunohistochemical study showed a low expression of p-53 and Mcm-2 in PA and RPA and an increased expression of these proteins in the RPA with areas of malignant transformation. © 2010 John Wiley & Sons A/S. Source


Mendonca J.A.,Sao Leopoldo Mandic Institute and Research Center | Francischone C.E.,Sao Leopoldo Mandic Institute and Research Center | Senna P.M.,Health Science University | De Oliveira A.E.M.,Federal University of Juiz de fora | Sotto-Maior B.S.,Federal University of Juiz de fora
Journal of Periodontology | Year: 2014

The aim of the present study is to evaluate the survival rate and bone loss around short implants (≥10 mm) supporting splinted or non-splinted posterior prostheses during a follow-up period of 3 to 16 years. Methods: A total of 453 implants from 198 patients was divided into splinted or non-splinted groups. Implant survival rate was calculated for each group, and potential risk was represented as odds ratio (OR). The final linear distance from implant platform level to the first bone-to-implant contact was compared to this same reference just after loading by digital periapical radiographs to determine the marginal bone loss (BL). Results: The splinted group comprised 219 implants in 86 patients, whereas the non-splinted group included 234 implants from 112 patients. The mean follow-up period was 9.7 ± 3.7 years. Although different success rates were found for splinted (97.7%) and non-splinted (93.2%) groups, they exhibited similar BL (1.22 ± 0.95 mm and 1.27 ± 1.15 mm, respectively). The success of splinted implants was associated with no other variable, whereas non-splinted implants exhibited higher risk of failure when placed in men (OR = 3.2) and when implants shorter than 10 mm were used (OR = 3.6 and 4.1 for 8.5 mm and 7 mm, respectively). Regardless of group, 71.4% of the unsuccessful implants failed before the end of the first year after loading. Conclusion: Non-splinted posterior short implants had a somewhat lower success rate than splinted short implants, and the failure rate in non-splinted short implants appeared to be greater in males as well as in implants ≥10 mm. Source


Soares A.B.,Sao Leopoldo Mandic Institute and Research Center | Demasi A.P.D.,Sao Leopoldo Mandic Institute and Research Center | Altemani A.,University of Campinas | De Araujo V.C.,Sao Leopoldo Mandic Institute and Research Center
Histopathology | Year: 2011

Aims: Pleomorphic adenoma (PA) is the most common salivary gland tumour with a tendency to recur (RPA) and a risk of malignant transformation. Mucin 1 (MUC-1) plays a role in the progression of many tumours and may be a marker to predict RPA. The aim of this study was to evaluate MUC-1 expression in different phases of the adenoma to carcinoma sequence. Methods and results: Twenty-one cases of PA, 18 cases of RPA, three cases of RPA with focal transformation (TRPA) and 11 cases of carcinoma ex-pleomorphic adenoma (CXPA) were analysed immunohistochemically for MUC1 expression using an antibody to MUC1/DF3. MUC1 reactivity in RPA was stronger than that observed in PA and, in all the different carcinoma groups, MUC-1 expression was significantly higher in carcinoma than in RPA and PA. Conclusion: This study has confirmed that MUC-1 is related to the recurrence of PA and that this molecule is associated with malignant transformation of PA with carcinoma cells overexpressing MUC-1. © 2011 Blackwell Publishing Limited. Source


Martinez E.F.,Sao Leopoldo Mandic Institute and Research Center | Demasi A.P.D.,Sao Leopoldo Mandic Institute and Research Center | Napimoga M.H.,Sao Leopoldo Mandic Institute and Research Center | Arana-Chavez V.E.,University of Sao Paulo | And 3 more authors.
Oral Oncology | Year: 2012

In order to investigate the role of myoepithelial cell and tumor microenvironment in salivary gland neoplasma, we have performed a study towards the effect of different extracellular matrix proteins (basement membrane matrix, type I collagen and fibronectin) on morphology and differentiation of benign myoepithelial cells from pleomorphic adenoma cultured with malignant cell culture medium from squamous cell carcinoma. We have also analyzed the expression of α-smooth muscle actin (α-SMA) and FGF-2 by immunofluorescence and qPCR. Our immunofluorescence results, supported by qPCR analysis, demonstrated that α-SMA and FGF-2 were upregulated in the benign myoepithelial cells from pleomorphic adenoma in all studied conditions on fibronectin substratum. However, the myoepithelial cells on fibronectin substratum did not alter their morphology under malignant conditioned medium stimulation and exhibited a stellate morphology and, occasionally focal adhesions with the substratum. In summary, our data demonstrated that the extracellular matrix exerts an important role in the morphology of the benign myoepithelial cells by the presence of focal adhesions and also inducing increase FGF-2 and α-SMA expression by these cells, especially in the fibronectin substratum. © 2011 Elsevier Ltd. All rights reserved. Source

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