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Pinto C.F.,Universidade Sao Francisco | Berger S.B.,University of Northen Parana | Cavalli V.,Sao Leopoldo Mandic Dental School and Research Center | Bedran-Russo A.K.,University of Illinois at Chicago | Giannini M.,University of Campinas
International Journal of Adhesion and Adhesives | Year: 2015

Objectives. The objective of this study was to evaluate the effect of chemical collagen cross-linkers on the bond strength of three self-etching adhesives to dentin. Materials and methods. Exposed dentin from the buccal surface of 45 incisors bovine was used to analyze the effect of chemical cross-linkers. The control groups were tested with self-etching adhesives (Clearfil SE Bond, Clearfil SE Protect and One-up Bond F Plus) according to the manufacturer instructions. Two cross-linkers agents were tested: 5% glutaraldehyde and 6.5% proanthocyanidin-rich grape seed extract (both for 10 min). After surface treatments with the agents, the surfaces were washed with distilled water, followed by the bonding/build-up procedures. Restored teeth were prepared for microtensile bond strength test and specimens tested in a universal testing machine (0.5 mm/min) after 24 h storage. Fracture sites of the bonded interface qualitatively evaluated. Results. According to two-way ANOVA and Tukey test (p<0.05) glutaraldehyde pretreatment did not affect the microtensile bond strength of any of two self-etching systems (p>0.05). However, when the grape seed extract was used with Clearfil SE Bond, the dentin bond strength values increased (p<0.05), but decrease for the One-up Bond F Plus treated-group (p<0.05). For the Clearfil SE Protect there was no difference between treatments (p>0.05). Conclusions. The effect of the application of grape seed extract cross-linker was product-dependent and glutaraldehyde did not affect the bond strength to dentin. © 2015 Elsevier Ltd. All rights reserved.

De Oliveira E.L.,Sao Leopoldo Mandic Dental School and Research Center | Freitas F.F.,University of Campinas | De Macedo C.G.,University of Campinas | Clemente-Napimoga J.T.,University of Campinas | And 4 more authors.
Archives of Oral Biology | Year: 2014

Objective Low dose propranolol has previously been demonstrated to suppress bone remodelling. Therefore, its effect on orthodontic movement was tested. Design Rats were assigned as follows (n = 5): animals with no orthodontic appliance (G1); the remaining groups were fitted with a Ni-Ti closed-coil spring ligated to the upper left first molar and connected to the incisors using metal and resin and received vehicle only (G2), 0.1 mg/kg (G3) or 20 mg/kg (G4) of propranolol orally. Cone Beam Computed Tomography was performed using high resolution for image capture. The distance between the first and second upper molars, both with and without the orthodontic appliance, was measured in millimetres. Gingival tissue was harvested and assessed for IL-1β and IL-6 using ELISA and for ICAM-1 and RANKL by Western blotting. Results The orthodontic appliance induced a significant tooth movement in G2 when compared to the animals without an orthodontic appliance (G1) (p < 0.05). The animals from G3 showed a significantly reduction in tooth movement (p < 0.05) when compared with rats from G2. Animals treated with 20 mg/kg of propranolol (G4) showed tooth movement similar to that of G2. The reduced tooth movement observed in the animals treated with 0.1 mg/kg of propranolol (G3) occurred due to decreased amounts of IL-1β and IL-6, in addition to lower ICAM-1 and RANKL expression. Conclusions Low dose propranolol inhibits bone remodelling and orthodontic movement. © 2014 Elsevier Ltd.

Fernandes A.C.R.,Sao Leopoldo Mandic Dental School and Research Center | Bridi E.C.,Sao Leopoldo Mandic Dental School and Research Center | Do Amaral F.L.B.,Sao Leopoldo Mandic Dental School and Research Center | Franca F.M.G.,Sao Leopoldo Mandic Dental School and Research Center | And 2 more authors.
International Journal of Adhesion and Adhesives | Year: 2014

Objectives The aim of this study was to evaluate the bond strength of methacrylate-based (Filtek Z350-3M/ESPE) or silorane-based (Filtek P90-3M/ESPE) resin composites associated with self-etching adhesive systems (Adper SE Plus-3M/ESPE, Adper P90-3M/ESPE and Clearfil SE Bond-Kuraray) or with a conventional adhesive system (Scotchbond MultiUso-3M/ESPE) to dentin after 24 h and 180 days storage times, and also to determine the fracture pattern. Materials and methods The occlusal enamel was removed from 48 recently extracted impacted third molars to expose the superficial dentin. The teeth were divided into eight groups (n=6) according to the adhesive system applied and to the resin composite used for block fabrication. The specimens were cut into sticks with an area of approximately 1 mm © 2013 PublishedbyElsevierLtd.

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