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Yang G.H.,Hainan University | Yang G.H.,Sanya Science and Technology Academy for Crop Winter Multiplication | Liu J.P.,Hainan University
Genetics and Molecular Research | Year: 2014

A full-length cDNA of a 1-aminocyclopropane-1-carboxylate synthase (ACS) family member from Oncidium, named OnACS1 (GenBank accession No. JQ822087) was cloned and characterized by reverse transcription polymerase chain reaction and rapid amplification of cDNA ends technology. The full-length cDNA was 1586 bp, including a 1308-bp open reading frame, a 105-bp 5' untranslated region (UTR), and 173-bp 3' UTR, encoding 436 amino acids. The deduced amino acid sequence of OnACS1 shares 85, 84, and 83%homology with ACS proteins of Cattleya bicolor, Dendrobium crumenatum, and Phalaenopsis hybrid, respectively. Prokaryotic expression and sodium dodecyl sulfate polyacrylamide gel electrophoresis showed that a specific band was produced and was consistent with the predicted protein size. A tissue-specific manner of OnACS1 expression was observed, and it was predominantly expressed in the gynostemium. The OnACS1 expression in the sepals and gynandria was upregulated by 1% ethephon treatment. © FUNPEC-RP. Source


Yang X.F.,Sanya Science and Technology Academy for Crop Winter Multiplication | Liang H.Y.,Zhengzhou University | Ummin O.,Zhengzhou University | Yang M.,Sanya Science and Technology Academy for Crop Winter Multiplication
Advanced Materials Research | Year: 2014

The application of new polymer materials in modern agriculture is considered and discussed in a completely new view in this paper. Combined with modern agricultural characteristics: out of season and large-scale planting, the excellent characteristics of modern polymeric materials are skillfully applied to modern agriculture greenhouse rack, which solves many defects of the traditional greenhouse frame structure material. This paper takes Hainan Province as the area of greenhouse structure research and analyze the shed load in the view of structural analysis, based on which the finite element software ABAQUS for the greenhouse framework structure are analyzed by finite element analysis. The results show that the stress and deformation results can satisfy the requirements of structural strength in the normal load conditions. © (2014) Trans Tech Publications, Switzerland. Source


Ke Y.-C.,Sanya Science and Technology Academy for Crop Winter Multiplication | Wang S.,Sanya Science and Technology Academy for Crop Winter Multiplication | Ren H.,Sanya Science and Technology Academy for Crop Winter Multiplication | Zhang J.-B.,Nanjing Normal University | Cai Z.-C.,Nanjing Normal University
Chinese Journal of Ecology | Year: 2014

In this article, we studied the effects of intensively reductive treatment on soil physical, chemical and biological properties in a watermelon continuous cropping obstacle field in Hainan Province. The experiment contained four treatments, i. e. CK (no flooding), flooding, flooding incorporated with plastic film mulching, and flooding incorporated with plastic film mulching and 7500 kg·hm-2 alfalfa (Medicago sativa L.) (i. e. intensively reductive treatment). The results showed that the percentage of soil large clods (>5 mm) significantly decreased, suggesting that soil structure could be improved effectively by the intensively reductive treatment. The soil Eh value and nitrate concentration decreased rapidly, while the pH value increased obviously during the intensively reductive treatment. After the intensively reductive treatment, fungi and Fusarium oxysporum in soil reduced significantly. Our results indicated that the intensively reductive treatment may be a high efficient and environment-friendly method to improve the continuous cropping obstacle soil. Source


Han X.Y.,Sanya Science and Technology Academy for Crop Winter Multiplication | Huang Q.W.,Sanya Science and Technology Academy for Crop Winter Multiplication | Kong X.Y.,Sanya Science and Technology Academy for Crop Winter Multiplication | Luo F.,Sanya Science and Technology Academy for Crop Winter Multiplication | And 3 more authors.
Acta Horticulturae | Year: 2015

In the study hami melon cultivar 'Xizhoumi No. 25' was grafted onto 23 different types of cucurbit rootstocks (Cucurbita moschata, C. maxima, C. pepo, C. maxima × C. moschata, and Cucumis melo) and the self-grafted melon anvil, to study the compatibility of different rootstocks, and the effect of grafting on melon yield and quality. In terms of compatibility, C. maxima × C. moschata F1 was better than the melon-self anvil, C. moschata, and C. pepo compared with the self-rooted control. The differences in fruit quality and yield of grafted plants were non-significant, only few grafted cultivars slightly improved fruit quality and yield. The self-rooted plants presented good fruit characteristics, whereas grafted plants were affected although performance varied among the treatments. Based on graft compatibility, yield, and fruit quality assessment results, we concluded that 'Jiachenzhen No. 2' (S38), 'Jinanchangqingteng' (S27), 'Syngenta BN224' (S30), and 'Jiuquandabaizi' (S35) showed better performances than other rootstocks, whereas C. maxima, 'Nangua No. 1', C. moschata, 'Huofenghuang' (S25), and 'Zhuangshi' (S31) proved to be inferior and these rootstock are not recommended for 'Xizhoumi No. 25'. Source


Kong X.Y.,Sanya Science and Technology Academy for Crop Winter Multiplication | Wang S.,Sanya Science and Technology Academy for Crop Winter Multiplication | Wan S.L.,Sanya Science and Technology Academy for Crop Winter Multiplication | Xiao C.L.,Sanya Science and Technology Academy for Crop Winter Multiplication | And 2 more authors.
Plant Disease | Year: 2015

In December 2014, Sanya City (18°N) of Hainan Province, the southernmost city of China, suffered an onslaught of downy mildew over large basil-growing areas. According to our investigation, almost 40% of the plants in the region (70 km2) were infected by the disease, which spread very rapidly. Given favorable conditions, the disease rate could reach 100% three to five days after the attack, making prevention and control extremely difficult. The seedling and adult plant stages of basil are subject to infection by the disease. In the early stages of the disease, the infected parts of the green leaf blades turn yellow, and then brown spots develop at the leaf margins. In the later stages of the disease, the spots spread along the margins or main veins of the leaves into larger areas, amassing as irregular, brown, necrotic spots that can consequentially wither the blades, and more seriously, cover more than half of the leaves. Meanwhile, the leaf back can be completely covered with a grayish-black layer of mildew. In the final stages of the disease, all the leaves of the plant will be infected, whereupon they will turn brown. When examined under a microscope, large amounts of sporangiophores and sporangia were found in the samples of the mildew layer collected from the infected leaves. Sporangiophores were hyaline, 197.0 to 595.3 µm (average 370.6 µm) in length, branched two to seven times, and ending with two slightly curved branchlets having a single sporangium at the tip of each branchlet. The longer branchlets measured 14.3 to 38.3 µm in length (average 23.2 µm), while the shorter ones were 2.4 to 22.1 µm in length (average 8.1 µm). Sporangia were oval or circular, ranged from brown to dark brown, measured 19.8 to 33 µm in length (average 27.6 µm) and 15.1 to 29.3 µm in width (averaging 23.0 µm) (a length/width ratio of 1.2), and produced germ tubes by direct germination. Based on these morphological characteristics, the causal agent was identified as Peronospora belbahrii Thines (Belbahri et al. 2005; Thines et al. 2009). Furthermore, genomic DNA was extracted from infected plant tissue according to Dellaporta et al. (1983). The complete ITS rDNA region was amplified and sequenced using primers ITS5 and ITS4 (Nagy and Horvath 2011). The consensus sequences were deposited in GenBank (Accession No. KP657570), and a BLAST analysis in the NCBI database revealed 99% similarity to all of the P. belbahrii sequences previously reported in regard to basil (EU863410 and KJ960193). For the preparation of the inoculum, infected leaves were flooded with sterile distilled water to collect the sporangia. The sporangia were washed once with sterile distilled water and the suspension was diluted to 1 × 105sporangia/ml. The sporangia suspension was sprayed onto 15 healthy leaves grown in the same period for inoculation, and sterilized distilled water was sprayed on 15 healthy leaves serving as controls. The experiment was repeated 10 times. Every replicate was placed in a separate petri dish under 12 h of light at 20°C, and 90 to 95% relative humidity. Five days after inoculation, the leaves inoculated with the suspension showed typical symptoms of the disease while the control leaves showed no symptoms. Infected leaves under the microscope showed large amounts of P. belbahrii fungi. Therefore, all of the results suggest that the downy mildew on basil was caused by the fungus P. belbahrii. To our knowledge, this is first report of downy mildew on basil in China. © The American Phytopathological Society. Source

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