Entity

Time filter

Source Type


Sharma P.,Santosh Medical College and Hospital Santosh University | Kumar P.,Santosh Medical College and Hospital Santosh University | Sharma R.,Dr M C Sexena Institute Of Medical Science And Hospital | Dhot P.S.,Santosh Medical College and Hospital Santosh University
Asian Journal of Pharmaceutical and Clinical Research | Year: 2015

Inborn errors of metabolism (IEM) arise from inherited errors in enzyme in any of the metabolic pathways. Symptoms are developed due to the accumulation of biosynthetic precursors or product that baby cannot excrete. IEM screening is a field that is undergoing a revolutionary change in its all aspects. Technological advances are transforming the way in which we can diagnose the whole population of IEM. In last few decades, evolution of gas chromatography-mass spectrometry (GC-MS) has improved our diagnostic capabilities in many fields. It is now possible to screen rapidly, simultaneously and inexpensively for a number of very rare disorders using GC-MS. Recent developments in the field of analytical and separation science and MS has brought about great improvement in GC-MS efficiency and sensitivity. The use of this technique in newborn screening made it possible in the present scenario, to screen for a wide range of previously unscreened IEM, including aminoacidemias, fatty acid oxidation disorders, and organic acidemias. Early diagnosis at appropriate time may help to decrease mortality and morbidity rates in children with IEM. In fact, the technique has tremendously improved the efficacy of neonatal screening programs. Uses of GC-MS demonstrate the importance of early identification and treatment of infants with disorders that would if go unrecognized, lead to irreversible clinical damage. The growing realization of the significant role of GC-MS in IEM screening resulted in extensive research in this area. Several excellent publications over the recent years, describe the crucial role played by the technique in fast high-throughput screening. The contributions by Matsumoto and Khurana together with the most recent work are the excellent areas for anyone intend to enter this field. © 2015, Asian Journal of Pharmaceutical and Clinical Research. All rights reserved.


Gupta G.,Santosh Medical College and Hospital Santosh University | Sharma P.,Santosh Medical College and Hospital Santosh University | Kumar P.,Santosh Medical College and Hospital Santosh University | Sharma R.,TSM Hospital and Medical College
Asian Journal of Pharmaceutical and Clinical Research | Year: 2015

Subclinical hypothyroidism (SCH) and inflammatory diseases are now a day’s one of the most popular topics of research. Previous studies have shown that the patients with SCH have increased levels of triglycerides and signs of low-grade inflammation (raised C-reactive protein levels). Disorder might be a risk factor for the development of cardiovascular and other inflammatory diseases. However, there is still some controversy concerning the inflammatory impact of SCH. Treating patients with thyroid stimulating hormone values of <10 mIU/L is not compelling, except in pregnant women. Fortifying the association between SCH and inflammation and a better understanding of research data may provide a more compelling argument for future treatment. © 2014 All Rights Reserved.


Singhal P.,Santosh Medical College and Hospital Santosh University | Sharma P.,Santosh Medical College and Hospital Santosh University | Kumar P.,Santosh Medical College and Hospital Santosh University | Sharma R.,Integral Institute of Medical science
Indian Journal of Public Health Research and Development | Year: 2014

The present study was carried out on 40 healthy volunteers, to analyze their SGPT (serum glutamate pyruvate transaminase) and uric acid level in the serum. The volunteers had the access to light meal containing a standard amount of carbohydrate, protein and lipid. Blood was taken for analysis of aforesaid parameters, before the meal and 1, 2, & 4 hours thereafter. Clinically significant variations were observed for SGPT at different time intervals post prandial. However uric acid concentration remains unchanged at all the time intervals. Results of the study clearly indicate that requirement of the patient on fasting for uric acid estimation is of no concern, while variation of SGPT level with time after light meal demonstrate that fasting time needs to be carefully considered in order to interpret the results correctly. © 2014 Indian Journal of Public Health Research and Development. All rights reserved.


Gupta G.,Santosh Medical College and Hospital Santosh University | Sharma P.,Santosh Medical College and Hospital Santosh University | Kumar P.,Santosh Medical College and Hospital Santosh University | Itagappa M.,Santosh Medical College and Hospital Santosh University
Journal of Clinical and Diagnostic Research | Year: 2015

Introduction: Subclinical hypothyroidism shows the mimic reaction more like to frank hypothyroidism which creates the dilemma. Inflammatory markers can be helpful in assessment of adverse effects of subclinical hypothyroidism, are not very well studied in the past. So the aim of this study was to investigate the role of inflammatory markers in Subclinical hypothyroidism patients.Materials and Methods: The study population consisted of 154 patients with recently diagnosed subclinical hypothyroidism and 100 healthy controls. TSH, FT4 & T3 were estimated by enzyme linked Immunosorbent assay (ELISA) for diagnosis of subclinical hypothyroidism. Total cholesterol, triglycerides, and HDL-C were estimated by spectrophotometric method. LDL – C was calculated by Friedewald formula. Inflammatory markers (ESR, C-reactive protein & Interleukin 6) were also estimated by enzyme linked Immunosorbent assay (ELISA).Results: In this study the level of TSH Mean ± SD (11.12±4.17 vs 2.73±0.80) and T3 Mean ± SD (0.96±0.17 vs 1.08±0.26) were significantly higher (<0.001) in subclinical hypothyroidism. Serum concentration of FT4 was not significantly different between the groups. Total cholesterol, triglycerides, and LDL-C were significantly higher in patients group. While the level of HDL-C was significantly lower in SCH patients compared to euthyroid group. TSH level was positively correlated with inflammatory markers in subclinical hypothyroidism, which were significantly different in subclinical hypothyroidism.Conclusion: This study suggests that subclinical hypo-thyroidism patients have increased inflammatory markers along with dyslipidemia and due to that future risk of further development of cardiovascular disorder can occur. Level of inflammatory markers increases in patients as disease progress if left untreated. © 2015 Journal of Clinical and Diagnostic Research All rights reserved.

Discover hidden collaborations