Ōnojō, Japan
Ōnojō, Japan

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Iga K.,Sansho Seiyaku Co. | Yokota S.,Sansho Seiyaku Co. | Nakai D.,Sansho Seiyaku Co. | Nakayama H.,Meguro Chen Dermatology Clinic | Chen K.-R.,Meguro Chen Dermatology Clinic
Nishinihon Journal of Dermatology | Year: 2015

We have previously conducted clinical studies using Kojic Acid (KA) to treat melasma, solar lentigo, nevus Ota, ephelides, pigmented cosmetic dermatitis, and other pigmentation disorders over the past 35 years. Interestingly, many of the patients treated with KA showed improvement not only in pigmentation but also in yellowish hue and reduced-translucency of the skin, here termed'yellowish discoloration'. Recently, advanced glycation end products (AGEs) produced by glycation of proteins, and advanced lipoxidation end products (ALEs) produced by carbonyl modification of proteins have been considered to be a cause of yellowish discoloration. The clinical evidence of the effect of KA on improving yellowish discoloration of the skin encouraged us to investigate the effect of KA on AGEs/ALEs production, and we have found that KA plays an important role in inhibiting AGEs/ALEs production in both the epidermis and dermis. Therefore, it is suggested that KA treatment can effectively produce improvement not only in pigmentation disorders but also in those with yellowish discoloration of the skin.

Terabayashi Y.,Japan National Institute of Advanced Industrial Science and Technology | Sano M.,Kanazawa Institute of Technology | Yamane N.,Japan National Institute of Advanced Industrial Science and Technology | Marui J.,Japan National Institute of Advanced Industrial Science and Technology | And 11 more authors.
Fungal Genetics and Biology | Year: 2010

Kojic acid is produced in large amounts by Aspergillus oryzae as a secondary metabolite and is widely used in the cosmetic industry. Glucose can be converted to kojic acid, perhaps by only a few steps, but no genes for the conversion have thus far been revealed. Using a DNA microarray, gene expression profiles under three pairs of conditions significantly affecting kojic acid production were compared. All genes were ranked using an index parameter reflecting both high amounts of transcription and a high induction ratio under producing conditions. After disruption of nine candidate genes selected from the top of the list, two genes of unknown function were found to be responsible for kojic acid biosynthesis, one having an oxidoreductase motif and the other a transporter motif. These two genes are closely associated in the genome, showing typical characteristics of genes involved in secondary metabolism. © 2010 Elsevier Inc.

Marui J.,Japan National Institute of Advanced Industrial Science and Technology | Marui J.,Japan National Food Research Institute | Yamane N.,Japan National Institute of Advanced Industrial Science and Technology | Ohashi-Kunihiro S.,Japan National Institute of Advanced Industrial Science and Technology | And 12 more authors.
Journal of Bioscience and Bioengineering | Year: 2011

A gene encoding the Zn(II) 2Cys 6 transcriptional factor is clustered with two genes involved in biosynthesis of a secondary metabolite, kojic acid (KA), in Aspergillus oryzae. We determined that the gene was essential for KA production and the transcriptional activation of KA biosynthetic genes, which were triggered by the addition of KA. © 2011 The Society for Biotechnology, Japan.

Nakayama H.,Nakayama Dermatology Clinic | Chen K.-R.,Saiseikai Central Hospital | Iga K.,Sansho Seiyaku Co. | Jinnai H.,Sansho Seiyaku Co.
Nishinihon Journal of Dermatology | Year: 2015

For the purpose of treatment for hyperpigmentation, such as melasma, pigmented cosmetic dermatitis (PCD), dirty neck and other various postinflammatory pigmentation which did not easily disappear spontaneously were treated by the application of 0.4% kojic acid plus 0.05% liquiritin (0.45KL II cream) every day. The effect was followed up monthly, with the photographs taken from the same angle and distance using the same camera and color films. Results: melasma was improved at the rate of 94.4%, including 34 patients of complete cure and improvement when 0.45KL II cream used on average 10 months. PCD patients were patch tested using current cosmetic series patch test allergens, then they were requested to exclusively use the cosmetics and soaps which did not contain causative allergens to remove the crucial effect of various cosmetic allergens. Then they were requested to use 0.45KL II cream to speed up the improvement of hyperpigmentation. 16 out of 17 PCD patients were improved or cured at the rate of 94.1%. Only one Indonesian severe PCD patient who had multiple sensitization showed incompatibility to this whitening agent. 17 out of 19 postinflammatory hyperpigmentation including dirty neck showed improvement at the rate of 89.5%. 0.45KL II cream was concluded as to be very effective to the above mentioned hyperpigmentation with excellent adaptability. Production of leukoderma has never been recorded during 30 years of various formula of kojic acid.

Ohshima E.,Mishima Institute for Dermatological Research | Ohshima E.,Sansho Seiyaku Co. | Higa Y.,Mishima Institute for Dermatological Research | Higa Y.,Sansho Seiyaku Co. | Mishima Y.,Mishima Institute for Dermatological Research
Skin Research | Year: 2011

A new cosmetic agent, "Souou Extract", inhibits melanin polymer formation of mouse B16 melanoma cells. In order to investigate the mechanism of the reduced pigmentation effect of Souou Extract, we analyzed changes in tyrosinase activities among subcellular compartments, and the inhibition effect on isolated tyrosinase activity, parallel with electron microscopic observation of less-pigmented B16 cells. Souou Extract has been found to induce no inhibition of tyrosinase activity ; however, less-pigmented cells showed a significant decrease of T3-tyrosinase and melanin polymer in premelanosomes, and selective structural aberration of premelanosomes. Tyrosinase synthesized by ribosomes is known to be glycosylated in the Golgi-associated endoplasmic reticulum of lysosomes (GERL) and coated vesicles (CV), and transferred into premelanosomes ; however, in Souou Extract-treated B16 cells, the GERL-CV system showed no distinct decrease in their dopa reaction. These results indicate that the mechanism of the inhibitory action of Souou Extract on melanogenesis is the inhibition of tyrosinase glycosylation in the GERL-CV system of pigment cells.

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