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Jiang J.-L.,Sanming Academy of Agricultural Science | Ye W.,Sanming Academy of Agricultural Science | Li Y.-Q.,Sanming Academy of Agricultural Science | Zhou J.-J.,Sanming Academy of Agricultural Science | And 2 more authors.
Zhiwu Shengli Xuebao/Plant Physiology Journal | Year: 2014

Polyploidy Dendrobium officinale was induced by colchicine. Autotetraploid plantlets were screened by appearance, stoma and chromosome number. The accumulation of polysaccharides and growth rate in autotetraploid were also analyzed. Results indicated that, the contents of polysaccharides in stem, leaf, and protocorm-like body of autotetraploid were 1.31, 1.83 and 1.95 fold to diploid, but the growth rate were significantly or very significantly lower than the control. Obviously genomic mutations were observed by ISSR molecular marker analysis in autotetraploid plants. Source


Ye W.,Fujian Agriculture and forestry University | Ye W.,Sanming Academy of Agricultural Science | Shen C.-H.,National Taiwan University | Shen C.-H.,Industrial Technology Research Institute of Taiwan | And 6 more authors.
PLoS ONE | Year: 2014

Piriformospora indica, an endophytic fungus of Sebacinales, colonizes the roots of a wide range of host plants and establishes various benefits for the plants. In this work, we describe miRNAs which are upregulated in Oncidium orchid roots after colonization by the fungus. Growth promotion and vigorous root development were observed in Oncidium hybrid orchid, while seedlings were colonized by P. indica. We performed a genome-wide expression profiling of small RNAs in Oncidium orchid roots either colonized or not-colonized by P. indica. After sequencing, 24,570,250 and 24744,141 clean reads were obtained from two libraries. 13,736 from 17,036,953 unique sequences showed homology to either 86 miRNA families described in 41 plant species, or to 46 potential novel miRNAs, or to 51 corresponding miRNA precursors. The predicted target genes of these miRNAs are mainly involved in auxin signal perception and transduction, transcription, development and plant defense. The expression analysis of miRNAs and target genes demonstrated the regulatory functions they may participate in. This study revealed that growth stimulation of the Oncidium orchid after colonization by P. indica includes an intricate network of miRNAs and their targets. The symbiotic function of P. indica on Oncidium orchid resembles previous findings on Chinese cabbage. This is the first study on growth regulation and development of Oncidium orchid by miRNAs induced by the symbiotic fungus P. indica.© 2014 Payne. Source

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