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Bangalore, India

Prasad M.P.,Sangenomics Research Labs | Prabhu A.,Bangalore University | Thakur M.S.,Bangalore University | Ruparel Y.M.,Bangalore University
International Journal of Pharma and Bio Sciences | Year: 2013

Plants have richest source of bioactive compounds as phytochemicals in the form of secondary metabolites. In this study three Cestrum species C. aurantiacum, C. nocturnum and C. diurnum were investigated for phytochemicals and revealed presence of Alkaloids, Anthraquinones, Cardiac Glycosides, Carbohydrates, Flavonoids, Phenolic compound, Tannins and Terpenoids. Antioxidant potential was estimated by DPPH and FRAP assay and C. diurnum showed higher antioxidant potential than that of the other two species in both assays. Different solvents such as ethanol, methanol, butanol, propanol and acetone were used to extract the bioactive compounds from the leaves of these plant species. The antimicrobial activity of these solvent extracts was tested against three bacterial (S. typhi, P. aeruginosa and K. pneumoniae) and two fungal species (Aspergillus and Trichoderma) by agar well diffusion method. C. aurantiacum showed maximum antibacterial activity, and maximum antifungal activity was detected for butanol extract of C. nocturnum against Aspergillus. Source

Prasad M.P.,Sangenomics Research Labs | Sekhar S.,Sangenomics Research Labs
International Journal of Pharma and Bio Sciences | Year: 2013

The present study was conducted for the isolation of a suitable Amylase producing Bacterial and Fungal strain and optimization of the cultural conditions for the production of amylase enzyme. Studies on the optimum conditions for the production of amylase were performed with isolated and identified bacterial (Bacillus) and fungal (Aspergillus) species. The optimum temperature for amylase production by bacterial species was detected as 35°C and by fungal species was detected as 25°C. Amylase production was observed at pH 5-9 with maximum at pH 7 for bacteria and pH 6 for fungus. The levels of amylase production varied greatly with the addition of carbon source. Effect of different nitrogen sources revealed that protease peptone and urea were the better nitrogen sources and showed increased enzyme yield. The amylase enzyme was purified by Ammonium sulphate precipitation followed by Dialysis and then column chromatography. The purified Amylase was then analyzed by SDS-PAGE. Source

Prasad M.P.,Sangenomics Research Labs | Manjunath K.,Sangenomics Research Labs
Indian Journal of Biotechnology | Year: 2011

Studies on bioremediation of high fat and oil wastewater by selected lipase producing bacteria like Bacillus subtilis, B licheniformis, B amyloliquefaciens, Serratia marsescens, Pseudomonas aeruginosa and Staphylococcus aureus were carried out in wastewaters emanating from palm oil mill, dairy, slaughter house, soap industry and domestic wastewater with both individual and mixed culture (consortia). BOD and lipase degradation was analyzed for 12 d. After 12 d of bioremediation, least BOD and lipid content was observed in consortia. Among the six isolates, P. aeruginosa showed least BOD (112 mg/L) in palm oil effluent, (82 mg/L) dairy effluent, (145 mg/L) soap and (9 mg/L) domestic water effluent, whereas S. aureus showed least BOD (11 mg/L) in slaughter house wastewater. Lipid content was also reduced most by consortia after 12 d of bioremediation. P. aeruginosa resulted in very good lipid degradation in palm oil effluent (325 mg/L), soap effluent (300 mg/L) and domestic wastewater (17 mg/L), whereas S. marsescens showed good lipid degradation in dairy effluents (280 mg/L) and S. aureus in slaughter house wastewater (320 mg/L). Source

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