Sangamo BioSciences and Ospedale San Raffaele srl | Date: 2015-01-07
Disclosed herein are methods and compositions for inactivating TCR genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain in conditions able to preserve cell viability. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided. Disclosed herein are also methods and compositions for expressing a functional exogenous TCR in the absence of endogenous TCR expression in T lymphocytes, including lymphocytes with a central memory phenotype. Polynucleotides encoding exogenous TCR, vectors comprising polynucleotides encoding exogenous TCR and cells comprising polynucleotides encoding exogenous TCR and/or cells comprising exogenous TCR are also provided.
Sangamo BioSciences | Date: 2015-12-10
Methods and compositions comprising single guide RNAs targeted to endogenous gene for genetic alteration of cells are provided.
Sangamo BioSciences | Date: 2015-04-29
Disclosed herein are linear donor molecules comprising homology arms of 50-750 base pairs (e.g., 50-100 base pairs) flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for targeted integration of an exogenous sequence into a specified region of interest in the genome of a cell.
Sangamo BioSciences | Date: 2015-08-21
Nucleases and methods of using these nucleases for expressing a transgene from a safe harbor locus in a secretory tissue, and clones and animals derived therefrom.
Sangamo BioSciences and Whitehead Institute For Biomedical Research | Date: 2015-10-29
Disclosed herein are methods and compositions for correction and/or mutation of genes associated with Parkinsons Disease as well as clones and animals derived therefrom.