Samsun Research and Education Hospital

Samsun, Turkey

Samsun Research and Education Hospital

Samsun, Turkey

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Acar U.,Hacettepe University | Pinarli F.A.,Diskapi Yildirim Beyazit Research and Education Hospital | Acar D.E.,Zekai Tahir Burak Womens Health Research and Education Hospital | Beyazyildiz E.,Samsun Research and Education Hospital | And 4 more authors.
Ophthalmic Research | Year: 2015

Aims: To investigate whether allogeneic limbal mesenchymal stem cell (LMSC) therapy affects corneal healing after a severe chemical burn and whether the route of administration of LMSCs differs in its therapeutic effect in this respect. Methods: A total of 60 Sprague-Dawley rats with clinically proven alkali injury were divided into four equal groups (n = 15) as follows: group 1: 2 × 105 cells/drop LMSCs, topically applied 6 times a day for 2 days; group 2: 2.4 × 106 cells in 0.5 ml LMSCs, subconjunctivally applied; group 3: 2.4 × 106 cells in 1 ml LMSCs, intraperitoneally applied, and group 4: no LMSC treatment. The groups were compared according to grades of corneal opacity (CO), corneal neovascularization (CNV) and corneal fluorescein staining (CFS). The migration of LMSCs into the cornea and the inflammatory characteristics of the groups were evaluated with BrdU (5-bromo-2′-deoxyuridine bromodeoxyuridine) immunostaining and histopathologically in a 4-week follow-up. Results: There were statistically significant differences between the LMSC-treated and control groups in each week regarding mean CO scores and in the 3rd week regarding the mean CNV and CFS scores (p < 0.05). The statistical significance was due to the differences between the topical and the control group and between the subconjunctival and the control group. BrdU+ LMSCs were seen in the corneal epithelium of the all LMSC-administered rats, and fewer inflammatory changes were observed in these rats. Conclusion: Allogeneic LMSC treatment, especially topical and subconjunctival administration, seems to be helpful in affecting corneal healing after a severe corneal burn. © 2015 S. Karger AG, Basel.


Cankaya A.B.,Hacettepe University | Kan S.,Diskapi Training and Research Hospital | Kizilgul M.,Diskapi Training and Research Hospital | Tokmak A.,Ulucanlar Eye Research Hospital | And 5 more authors.
International Ophthalmology | Year: 2016

The purpose of this study is to compare the corneal biomechanical properties in primary hyperparathyroid patients and healthy control subjects. The study consisted of 31 patients with primary hyperparathyroidism (study group) and 31 healthy subjects (control group). Corneal biomechanical properties, including corneal hysteresis (CH), corneal resistance factor (CRF), and intraocular pressure (IOP) were measured with an ocular response analyzer (ORA). IOP was also measured using Goldmann applanation tonometry (GAT), and central corneal thickness (CCT) was measured with an ultrasonic pachymeter. The differences in ORA parameters and CCT between study and control group participants were analyzed. The mean CH in study and control groups was 8.7 ± 1.9 mmHg (5.3–13.7 mmHg) and 9.8 ± 1.5 mmHg (7.7–14.3 mmHg), respectively (p = 0.018). The mean CRF was 9.5 ± 1.8 (5.5–13.7) in the study group compared with 9.8 ± 1.5 (6.2–12.8) in the control group. The difference for CRF was not statistically significant (p = 0.41). In study and control group, corneal-compensated IOP (IOPcc) values were 18.2 ± 4.2 and 16.9 ± 2.7 mmHg, respectively (p = 0.12). Mean IOP measurement values with GAT were 16.3 ± 3.4 mmHg for study group and 16.5 ± 2.7 mmHg for control group (p = 0.71). The mean differences of IOPcc and IOPGAT in the study group eyes were higher than that of control group eyes (1.9 vs. 0.4 mmHg). CCT was 536.5 ± 25.4 µm (490–593 µm) in study group eyes compared with 534.2 ± 31.4 µm (472–602 µm) in control eyes (p = 0.75). Hyperparathyroidism could be associated with a decrease of CH. The differences between IOPcc and IOPGAT in these patients were higher than normal subjects. Underestimation of IOP readings with GAT may be a consequence of the lower CH in patients with hyperparathyroididsm. © 2016 Springer Science+Business Media Dordrecht


PubMed | Ulucanlar Eye Research Hospital, Samsun Research and Education Hospital, Kastamonu University, Hacettepe University and Diskapi Training and Research Hospital
Type: | Journal: International ophthalmology | Year: 2016

The purpose of this study is to compare the corneal biomechanical properties in primary hyperparathyroid patients and healthy control subjects. The study consisted of 31 patients with primary hyperparathyroidism (study group) and 31 healthy subjects (control group). Corneal biomechanical properties, including corneal hysteresis (CH), corneal resistance factor (CRF), and intraocular pressure (IOP) were measured with an ocular response analyzer (ORA). IOP was also measured using Goldmann applanation tonometry (GAT), and central corneal thickness (CCT) was measured with an ultrasonic pachymeter. The differences in ORA parameters and CCT between study and control group participants were analyzed. The mean CH in study and control groups was 8.71.9mmHg (5.3-13.7mmHg) and 9.81.5mmHg (7.7-14.3mmHg), respectively (p=0.018). The mean CRF was 9.51.8 (5.5-13.7) in the study group compared with 9.81.5 (6.2-12.8) in the control group. The difference for CRF was not statistically significant (p=0.41). In study and control group, corneal-compensated IOP (IOPcc) values were 18.24.2and 16.92.7mmHg, respectively (p=0.12). Mean IOP measurement values with GAT were 16.33.4mmHg for study group and 16.52.7mmHg for control group (p=0.71). The mean differences of IOPcc and IOPGAT in the study group eyes were higher than that of control group eyes (1.9 vs. 0.4mmHg). CCT was 536.525.4m (490-593m) in study group eyes compared with 534.231.4m (472-602m) in control eyes (p=0.75). Hyperparathyroidism could be associated with a decrease of CH. The differences between IOPcc and IOPGAT in these patients were higher than normal subjects. Underestimation of IOP readings with GAT may be a consequence of the lower CH in patients with hyperparathyroididsm.

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