Wardhana,Sam Ratulangi University
Acta medica Indonesiana | Year: 2010
Recurrent Aphthous Stomatitis (RAS) is one of the most common oral lesions which occur either in single or multiple forms in oral mucosa. The mouth is subjected to a wide spectrum of antigenic agents, including foodstuff, and allergic reactions to such antigens may manifest in a number of diverse ways. Food allergy, however, has not been widely investigated as the cause of RAS. The main complaint of RAS typically is pain, and the main therapy is still corticosteroids, besides avoiding allergenic foodstuff. In RAS, there is often a genetic basis. More than 42 percent of patients with RAS have first-degree relatives with RAS. The likelihood of RAS is 90 percent when both parents are affected, but only 20 percent when neither parent has RAS, and it is also likely to be more severe and to start at an earlier age in patients with a positive family history. The primary goals of therapy of RAS are relief of pain, reduction of ulcer duration, and restoration of normal oral function. The secondary goals include reduction in frequency and severity of recurrences and maintenance of remission. Diagnostic elimination diets are frequently utilized both in diagnosis and management of RAS caused by food allergy. Patients with RAS may have increased levels of CD8+ T-lymphocytes and/or decreased CD4+ T-lymphocytes. There may be a reduced percentage of "virgin" T-cells and an increased of "memory" T-lymphocytes. Patients with active RAS have an increased proportion of gd T-cells compared with healthy control subjects and RAS patients with inactive disease. The gd T-cells may play a role in ADCC and it is believed that gd T-cells play a role in immunological damages. Preventive treatment is a consideration for patients with RAS caused by food allergy who report regular exacerbations of their condition. It focuses on dietary modifications, the earliest stage, the prodromal stage, and attempts to intercept ulcer development again by the use of topical immunosuppressant and particularly corticosteroids.
Yamagishi J.,Tohoku University |
Yamagishi J.,Obihiro University of Agriculture and Veterinary Medicine |
Natori A.,Tokyo Medical University |
Tolba M.E.M.,Assiut University |
And 9 more authors.
Genome Research | Year: 2014
To understand the molecular mechanisms of parasitism in vivo, it is essential to elucidate how the transcriptomes of the human hosts and the infecting parasites affect one another. Here we report the RNA-seq analysis of 116 Indonesian patients infected with the malaria parasite Plasmodium falciparum (Pf). We extracted RNAs from their peripheral blood as a mixture of host and parasite transcripts and mapped the RNA-seq tags to the human and Pf reference genomes to separate the respective tags. We were thus able to simultaneously analyze expression patterns in both humans and parasites. We identified human and parasite genes and pathways that correlated with various clinical data, which may serve as primary targets for drug developments. Of particular importance, we revealed characteristic expression changes in the human innate immune response pathway genes including TLR2 and TICAM2 that correlated with the severity of the malaria infection. We also found a group of transcription regulatory factors, JUND, for example, and signaling molecules, TNFAIP3, for example, that were strongly correlated in the expression patterns of humans and parasites. We also identified several genetic variations in important anti-malaria drug resistance-related genes. Furthermore, we identified the genetic variations which are potentially associated with severe malaria symptoms both in humans and parasites. The newly generated data should collectively lay a unique foundation for understanding variable behaviors of the field malaria parasites, which are far more complex than those observed under laboratory conditions. © 2014 Totoki et al.; Published by Cold Spring Harbor Laboratory Press.
Mamuaja C.T.,Sam Ratulangi University
International Journal of ChemTech Research | Year: 2015
In the last decade quite a number of interest have been shown in a healthier food products and one of its efforts was to modify the fatty acids components into an unsaturated one,such product is known as Lipid Specific Structured (LSS). Synthesis of lipid structured using interesterification process will be beneficial to improve the functional properties and nutritive value of fat and oil as expected in some processed products. Enzymatic interesterification of North Sulawesi skipjack(Katsuwonus palamis) fish oil which extracted using wet rendering process and regiospecific analysis using1H-NMR and13C-NMR to identify fatty acid in Sn1, 2 and 3 had been studied. The analysis results showed that in position Sn-1 and Sn-3 were subtituted by lauric acid and in position Sn-2 was still occupied by the fatty acid of skipjack fish oil such as the one contain ω-3 – PUFA. © 2015, Sphinx Knowledge House. All rights reserved.
Wardhana,Sam Ratulangi University
Acta medica Indonesiana | Year: 2011
Nowadays, people have been eating lots of unhealthy dietary excesses, that make them have chronic inflammatory diseases or known as chronic diseases. Countless millions of people worldwide can not help eating selectively massive quantities of unhealthy foods, until they become sick, often mortality. The omega-6 fatty acids account for the majority of PUFA (Poly Unsaturated Fatty Acids) in the food supply. They are the pre-dominant PUFA in all diets, especially the western diets, which produce pro-inflammatory metabolic products. The persistent antigenic or cytotoxic effects will lead to chronic inflammation. Olive tree is native to the Mediterranean basin and parts of Asia Minor. Its compression-extracted oil from the fruit has a wide range of therapeutic and culinary applications. It had been used as aphrodisiacs, emollients, laxatives, nutritives, sedatives, and tonics. In the later part of the 20th century, several studies had revealed that the olives in the Mediteranian diet is linked to a reduced incidence of degenerative diseases. It is one of phytomedicine which has omega-3 fatty acid as its constituent, may inhibit inflammation composing chronic inflammatory process in many chronic diseases, such as coronary artery disease, rheumatoid arthritis, hypertension, and even cancer.
Tilaar W.,Sam Ratulangi University
International Journal of Pharma and Bio Sciences | Year: 2014
The purpose of this study was to obtain new buds of kulo chrysanthemum by in vitro propagation technique or in vitro multiplication. It's also aimed to discover the influence of NAA and BAP in kulo chrysanthemum bud multiplication. This study used completely randomized design which was arranged by factorial with treatments which consisted of 0 ppm; 0,5 ppm; 1 ppm of NAA which were combined with 1 ppm; 2 ppm; 3 ppm of BAP. The explants used were kulo chrysanthemum nodules. Observed variables consisted of: budding time, callusing time, number of buds, height of buds, number of leaves, umber of roots, length of roots, percentage of grown cultures, percentage of unresponsive cultures, percentage of contaminated cultures. Data was analyzed using analysis of variance and continued by 5 % LSD test. The result was that there were interactions between NAA and BAP in their influence on number of buds, height of buds, number of roots, root length. NAA and BAP individually influenced the number of leaves.