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Dalvin S.,Norwegian Institute of Marine Research | Dalvin S.,Salmon Louse Research Center | Nilsen F.,Salmon Louse Research Center | Nilsen F.,University of Bergen | Skern-Mauritzen R.,Norwegian Institute of Marine Research
Journal of Natural History

Lepeophtheirus salmonis (salmon louse) is an ectoparasitic copepod. The salmon louse is an economically important pathogen in aquaculture and poses a serious threat to wild populations of salmonids. Whereas the ecology and biology of the salmon louse has been described to some extent, information on the molecular level is limited. A key step in the struggle to limit the number of salmon lice in farmed fish includes methods to limit reproduction in adult female lice. Here we describe a salmon louse homologue of the germ cell marker Vasa named Lepeophtheirus salmonis Vasa (LsVasa). Vasa, an RNA helicase, is a highly conserved gene found in germ cells in many organisms. The structure and sequence of LsVasa is described and localization and quantification of LsVasa transcripts is presented. © 2013 Copyright 2013 Institute of Marine Research, Bergen, Norway. Source

Skern-Mauritzen R.,Norwegian Institute of Marine Research | Skern-Mauritzen R.,Salmon Louse Research Center | Malde K.,Norwegian Institute of Marine Research | Besnier F.,Norwegian Institute of Marine Research | And 12 more authors.
Journal of Natural History

Molecular genetic tools have become standard in biological studies of both model and non-model species. This has created a growing need for sequence information, a resource hitherto limited for many species. With new sequencing technologies this is rapidly changing, and whole genome shotgun sequencing has become a realistic goal for many species. However, present sequencing protocols require more DNA than can be extracted from single individuals of many small metazoans, potentially forcing sequencing projects to perform sequencing on samples derived from several individuals. A pertinent question thus arises: can wild samples be used or is inbreeding necessary? In the present study we compare assemblies generated using sequence data from inbred and wild Lepeophtheirus salmonis. The results indicate not only that measures to reduce the genetic variability may significantly improve the final assemblies but also that deeper coverage to some extent can compensate for the detrimental effects of natural sequence variability. © 2013 Copyright Taylor and Francis Group, LLC. Source

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