Sakha Agricultural Research Station

Kafr ash Shaykh, Egypt

Sakha Agricultural Research Station

Kafr ash Shaykh, Egypt
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Yanni Y.G.,Sakha Agricultural Research Station | Dazzo F.B.,Michigan State University | Squartini A.,University of Padua | Zanardo M.,University of Padua | And 2 more authors.
Plant and Soil | Year: 2016

Aim of the research: This study examined whether rhizobia naturally associate with wheat (Triticum aestivum) and can enhance its production. Methods: Field-grown, surface-sterilized, macerated wheat roots were inoculated on legume roots to isolate rhizobial endophytes. Nodule occupants were purified, genotypically differentiated, and tested gnotobiotically for legume symbiotic effectiveness and wheat growth-promotion. Biofertilizer performance of 13 selected strains was evaluated on 8 wheat varieties in 24 field experiments in 10 counties of the Kafr El-Sheikh governorate in the Egypt Nile delta. Results: Only inoculated clover (Trifolium alexandrinum) produced root nodules hosting Rhizobium leguminosarum bv. trifolii. Some were symbiotically effective on clover and promoted wheat growth under gnotobiotic conditions. Inoculation significantly increased wheat grain yield in 21, 23, and 20 field experiments under N-application of 60, 120, and 180 kg N/ha, respectively, with corresponding increases of 9.2, 18.9, and 22.5 % higher grain yield over the mean of farmers’ yields using the same varieties in adjacent fields. Unlike the harvest indices, straw yield and agronomic fertilizer N-use efficiency benefitted from inoculation. Rhizobial mixed-strain inocula frequently outperformed single-strain inocula. Inoculation did not adversely affect endomycorrhizal infection of field-grown wheat roots. Conclusion: Rhizobium naturally develops an endophytic association with wheat. Selected biofertilizer strains can enhance wheat production with acceptable input/output economy. © 2016 Springer International Publishing Switzerland


Yanni Y.G.,Sakha Agricultural Research Station | Dazzo F.B.,Michigan State University
Plant and Soil | Year: 2010

This study assessed the ability of biofertilizer inoculants containing Rhizobium leguminosarum bv. trifolii to enhance production of rice (Oryza sativa L.) under actual agricultural conditions in the Nile delta. Large-scale field experiments evaluated 5 rice varieties inoculated with 7 endophytic rhizobial strains during 5 growing seasons, including at sites ranked as the world's highest in rice production. Inoculation with single strains or multi-strain consortia significantly increased grain yield in 19 of the 24 trials. By combining superior rhizobial inoculants with agricultural extension training, grain yield increased up to 47% in farmers' fields, with an average increase of 19. 5%. Data on rice straw production, harvest index and the agronomic fertilizer N-use efficiency also indicated positive agronomic benefits of rhizobial inoculation. These results establish the merit of deploying our biofertilization strategy using selected rhizobial strains to promote rice production capacity while reducing the need for additional chemical N-fertilizer inputs to maintain agricultural sustainability and acceptable production economy. Technology transfer of this important translational research can significantly help to alleviate hunger and meet the nutritional needs of many people in developing countries. © 2010 Springer Science+Business Media B.V.


Dazzo F.B.,Michigan State University | Klemmer K.J.,Michigan State University | Chandler R.,Michigan State University | Yanni Y.G.,Sakha Agricultural Research Station
Diversity | Year: 2013

This paper describes the utility of CMEIAS (Center for Microbial Ecology Image Analysis System) computer-assisted microscopy to extract data from accurately segmented images that provide 63 different insights into the ecophysiology of microbial populations and communities within biofilms and other habitats. Topics include quantitative assessments of: (i) morphological diversity as an indicator of impacts that substratum physicochemistries have on biofilm community structure and dominance-rarity relationships among populations; (ii) morphotype-specific distributions of biovolume body size that relate microbial allometric scaling, metabolic activity and growth physiology; (iii) fractal geometry of optimal cellular positioning for efficient utilization of allocated nutrient resources; (iv) morphotype-specific stress responses to starvation, environmental disturbance and bacteriovory predation; (v) patterns of spatial distribution indicating positive and negative cell-cell interactions affecting their colonization behavior; and (vi) significant methodological improvements to increase the accuracy of color-discriminated ecophysiology, e.g., differentiation of cell viability based on cell membrane integrity, cellular respiratory activity, phylogenetically differentiated substrate utilization, and N-acyl homoserine lactone-mediated cell-cell communication by bacteria while colonizing plant roots. The intensity of these ecophysiological attributes commonly varies at the individual cell level, emphasizing the importance of analyzing them at single-cell resolution and the proper spatial scale at which they occur in situ. © 2013 by the authors; licensee MDPI, Basel, Switzerland.


Yanni Y.,Sakha Agricultural Research Station | Zidan M.,Sakha Agricultural Research Station | Dazzo F.,Michigan State University | Rizk R.,Sakha Agricultural Research Station | And 3 more authors.
Agriculture, Ecosystems and Environment | Year: 2016

This study isolated and examined the performance of four selected strains of Rhizobium as growth enhancer inoculants of common bean (Phaseolus vulgaris) in saline- and drought-stressed fields located to the east and west of the Egypt Nile delta. Indigenous bean rhizobia were tested for salt tolerance by culturing and for taxonomic status by DNA analysis. Their nodulation and N2-fixation abilities under drought stress and persistence in biofertilizer formulations were evaluated, followed by assessment of their agronomic performance with common bean in 16 salt-affected, drought-stressed fields in combination with different doses of N-fertilizer applications in 2007 and 2008. Population dynamics studies with one model strain indicated good persistence in biofertilizer preparation. Inoculation with a test strain increased plant weight in the greenhouse from 2.718 to 3.314 g and four strains increased seed yield in saline/drought-stressed fields by 2.848–3.218 t ha−1 during seasons 2007 and 2008, respectively. Inoculation also increased straw production, harvest indices and the agronomic fertilizer N-use efficiency. The corresponding mean seed yields in adjacent uninoculated farmers’ fields exposed to the same intensity of aridification were 2.425 and 2.230 t ha−1, respectively. The study shows that locally-selected strains of rhizobia can be formulated into biofertilizers that significantly enhances seed yield and the agronomic N-use efficiency while providing a nature-based alleviation of the abiotic water deficit stress of intense aridification in saline- and drought-stressed fields. © 2016 Elsevier B.V.

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