Sakata Seed Sudamerica Ltda

São Paulo, Brazil

Sakata Seed Sudamerica Ltda

São Paulo, Brazil
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Barbosa J.C.,University of Sao Paulo | Costa H.,Instituto Capixaba Of Pesquisa | Gioria R.,Sakata Seed Sudamerica Ltda | Rezende J.A.M.,University of Sao Paulo
Tropical Plant Pathology | Year: 2011

Tomato chlorosis virus (ToCV) is a species in the genus Crinivirus, transmitted by Bemisia tabaci biotype B, first detected in Brazil in 2006 in the county of Sumaré, State of São Paulo. During the period from 2007 to 2010, RT-PCR analyses followed by nested-PCR with specific primers for ToCV detection, and sequencing of the amplicons, confirmed the presence of this virus in samples of symptomatic tomato plants (Solanum lycopersicum) from crops in counties in the states of Bahia (BA), Espírito Santo (ES), Goiás (GO), Minas Gerais (MG), and Rio de Janeiro (RJ). This is the first ToCV report for the states of BA, ES, GO, MG, and RJ, suggesting that this virus is widely disseminated in Brazil. Copyright by the Brazilian Phytopathological Society. Printed in Brazil.


Eckstein B.,Embrapa Recursos Geneticos e Biotecnologia | Barbosa J.C.,State University of Ponta Grossa | Kreyci P.F.,University of Sao Paulo | Zanol K.M.R.,Federal University of Paraná | And 5 more authors.
Australasian Plant Pathology | Year: 2014

Recently, disease in broccoli plants (Brassica oleracea var. italica) was associated with three distinct phytoplasmas in Brazil. The disease named broccoli stunt (BS) has caused significant economic losses in São Paulo State. Group 16SrIII phytoplasmas is the most common group that have been associated with BS. The BS disease is still poorly understood, and the lack of information about the vectors further impairs its management. In this study, leafhoppers belonging to 18 different species were collected from weeds thriving near broccoli fields that were affected by group 16SrIII phytoplasmas. Specific primers revealed the presence of group 16SrIII phytoplasmas in five leafhoppers: Empoasca spp., Agallia albidula Uhler, Agalliana sticticollis (Stål), Planicephalus flavicosta (Stål), and Atanus nitidus (Linnavuori). The identity of the phytoplasmas was confirmed through DNA sequencing analysis. The leafhoppers were infected by phytoplasmas of the 16SrIII group (proposed "Candidatus Phytoplasma pruni" species) and are phylogenetically related to the broccoli stunt phytoplasma (BSP) strains detected in the study area and, thus, are considered potential vectors of group 16SrIII phytoplasmas to broccoli plants. © 2014 Australasian Plant Pathology Society Inc.


Cabral C.S.,University of Pernambuco | Brunelli K.R.,Sakata Seed Sudamerica Ltda. | Costa H.,Incaper Venda Nova do Imigrante | Fonseca M.E.N.,Embrapa Hortalicas | And 2 more authors.
Tropical Plant Pathology | Year: 2014

The objective of this work was to assess the virulence to lettuce cultivars and host specificity and to identify races of Fusarium oxysporum f. sp. lactucae (FOLac) isolates associated with lettuce wilt in Brazil. Thirty-one isolates identified based on morphology were inoculated in a set of lettuce cultivars for race determination. Plantlets of 'Elisa', 'Vera', and 'Red Salad Bowl' cultivars were inoculated with all isolates using a root-dipping method. Isolates were also inoculated in species of Asteraceae and other botanical families. 'Elisa' and 'Vera' were susceptible to 27 isolates while 'Red Salad Bowl' was resistant to all isolates. Plants other than lettuce were not infected by any of the isolates, suggesting their specificity to lettuce. All pathogenic isolates were assigned to race 1 and used to evaluate a PCR protocol with primers targeting race 1. Only amplicons associated with race 1 pattern were observed for all pathogenic isolates but not for one non-pathogenic isolate, thus leading us to conclude that FOLac race 1 is so far the sole causal agent of lettuce wilt in Brazil. © by the Brazilian Phytopathological Society.


Eckstein B.,EMBRAPA - Empresa Brasileira de Pesquisa Agropecuária | Barbosa J.C.,University of Sao Paulo | Kreyci P.F.,University of Sao Paulo | Canale M.C.,University of Sao Paulo | And 2 more authors.
Journal of Phytopathology | Year: 2013

Since 2007, a new disease in broccoli (Brassica oleracea var. italica Plenck) has been observed in the São Paulo state, Brazil. The characteristic symptoms of the disease are plant stunting, inflorescence malformation, reddening of the leaves and phloem necrosis. Nested polymerase chain reaction with P1/Tint and F2n/R2 primer pairs revealed the presence of phytoplasmas in diseased broccoli plants. Restriction fragment length polymorphism and phylogenetic analysis of the 16S rDNA gene showed that phytoplasmas belonging to 16SrI, III and XIII groups were associated with the plants. To the best of our knowledge, this is the first report of phytoplasmas in this Brassica species in Brazil, as well the first time phytoplasmas of 16SrIII and XIII groups have been associated with broccoli plants. © 2013 Blackwell Verlag GmbH.


Fessel S.A.,Sakata Seed Sudamerica Ltda | Panobianco M.,Federal University of Paraná | de Souza C.R.,Federal University of Paraná | Vieira R.D.,São Paulo State University
Bragantia | Year: 2010

There are some results showing that the electrical conductivity test can be influenced by low temperature of seed storage, such as 10°C. This work was carried out in order to study the effect of temperature and period of storage on electrical conductivity and chemical composition of the imbibing solution of soybean seeds. For that, seed water content, germination, vigor (accelerated aging, cold test and electrical conductivity), and chemical composition (K+, Ca2+ and Mg2+) of the imbibing solution were determined. Two soybean seed lots of cultivar FT-20 with high and low vigor were used. The evaluations were performed at every three months intervals, during 15 months. Both temperature and storage period influenced seed vigor, as well as the ions leakage. It can be concluded that the electrical conductivity test is not suitable to evaluate vigor of soybean seeds stored at low temperature, and potassium is the major ion leakage regardless of the storage temperature.


dos Santos R.L.,São Paulo State University | dos Santos R.L.,Sakata Seed Sudamerica Ltda | Pavan M.A.,São Paulo State University | da Silva N.,São Paulo State University | Fumes L.A.A.,University of Sao Paulo
Summa Phytopathologica | Year: 2012

The inheritance of resistance to powdery mildew in the pea cultivar MK-10 and some histological aspects of infection were assessed. For the inheritance study, F1, F2, backcrosses and F3 generations of MK-10 crossed with two susceptible populations were evaluated. Histological evaluations included percentage of germinated conidia, percentage of conidia that formed appresoria, percentage of conidia that established colonies, and number of haustoria per colony. Segregation ratios obtained in the resistance inheritance study were compared by Chi-square (x1) test and the histological data were analyzed by Tukey's test at 5% probability. It was concluded that resistance of MK-10 to powdery mildew is due to a pair of recessive alleles since it is expressed in the pre-penetration stage and completed by post-penetration localized cellular death, characteristic of the presence of the pair of recessive alleles er1er1.


Lax P.,National University of Cordoba | Rondan Duenas J.C.,Laboratorio Of Biologia Molecular | Ramos D.,National University of Rio Cuarto | Doucet M.E.,National University of Cordoba | And 2 more authors.
Crop Protection | Year: 2016

The false root-knot nematode, Nacobbus aberrans, causes severe damage to field and greenhouse pepper crops in several localities of the American continent. No commercial peppers (Capsicum annuum) resistant to this nematode are available up to the present. Host suitability of 6 experimental and 3 commercial peppers (some of them carrying Me1 and Me7 resistance genes to Meloidogyne spp.) to two N. aberrans populations were evaluated under greenhouse experiments. None of the peppers was found to be resistant to the nematode. The evaluated parameters exhibited significant differences among some peppers tested within a single population and between populations for a single plant material. Some peppers carrying resistance showed higher nematode reproduction than some lines that did not possess resistance genes. These results confirmed that the genes conferring resistance to Meloidogyne spp. do not provide protection against this species of root-galling nematode. Host suitability of pepper lines carrying Me1 or Me7 resistance genes against N. aberrans is evaluated for the first time. Search for resistant genes against this nematode in wild peppers growing in areas where this nematode is indigenous should be promoted. © 2015 Elsevier Ltd.


Olinik J.R.,Av. Carlos Cavalcanti | Junior A.O.,Sakata Seed Sudamerica Ltda | Kepp M.A.,Sakata Seed Sudamerica Ltda | Reghin M.Y.,Av. Carlos Cavalcanti
Horticultura Brasileira | Year: 2011

The experiment was carried out in an area of Sakata Seed Sudamerica Ltda. in Bragança Paulista, Brazil, from August 22nd to October 11th, 2006, to evaluate different types of materials for soil covering and its effect on fruits with virus symptoms and on the yield of two hybrids of squash. The experimental design was randomized blocks, with four replications. The treatments followed a split-plot cheme, evaluating six soil coverings (black, silver and white polyethylene, black polypropylene, rice husk and bare soil) and two hybrids [(Novita Plus (cv. type Caserta) and Samira (cv. type Libanese)]. The technique of plastic soil covering, independently of the material used, was superior to bare soil. Among the materials the treatments using silver polyethylene promoted the highest yield and the lowest percentage of fruits with viruses in comparison to the other soil coverings. Among the hybrids, Novita Plus was superior to Samira in relation to the characteristics of fruit set, number of fruits per plants and yield, besides presenting 12% less fruits with virus symptoms in relation to Samira. For 'Nnovita Plus', the occurrence of virus symptoms began only at the eighth harvest on the treatments using silver and white polyethylene and rice husk. For 'Samira' the symptoms started at the second harvest on plants submitted to bare soil or black polypropylene and at fourth harvest on the other treatments.


Areas M.S.,São Paulo State University | Goncalves R.M.,São Paulo State University | Soman J.M.,São Paulo State University | Sakate R.K.,São Paulo State University | And 3 more authors.
Journal of Phytopathology | Year: 2015

Bacterial spot, caused by Xanthomonas spp., is one of the major diseases of pepper in Brazil, causing considerable losses to crop productivity. The efficient management of the disease is hampered by the high variability of the causal agents. In Brazil, there is no knowledge of which species of Xanthomonas occurs on pepper. In this study, 59 strains of Xanthomonas spp. isolated from different pepper-producing regions of Brazil were characterized by biochemical and molecular techniques. Results showed the prevalence of X. euvesicatoria as the causal agent of bacterial spot on pepper in Brazil. © 2015 Blackwell Verlag GmbH.


Cardoso A.I.I.,São Paulo State University | Claudio M.T.R.,Sakata Seed Sudamerica Ltda | Nakada-Freitas P.G.,São Paulo State University | Magro F.O.,Prefeitura de Jundiai | Tavares A.E.B.,São Paulo State University
Horticultura Brasileira | Year: 2016

Considering the scarce information on nutrient extraction by plants for seed production within vegetable crops, as well as the effect of fertilization over extraction, studying nutrient accumulation in different plant parts under varied fertilization rates is necessary. This study aimed to evaluate the influence of phosphate rates on macronutrient accumulation in cauliflower plants during seed production. Five phosphate rates were studied (0, 300, 600, 900 and 1200 kg/ha P2O5), in a randomized complete block design, with four replications. Accumulation of nutrients in the different plant parts was evaluated at the end of the cycle (vegetative and reproductive, the latter divided in seeds and inflorescence). Data were submitted to analysis of variance and regression. Considering only the seeds, a quadratic effect was obtained for the accumulation of all macronutrients, with maximum estimated for rates between 858 and 952 kg/ha P2O5. Considering the whole plant (total accumulation), a linear increase for P, Ca and S was obtained the higher the P2O5 rates, while the effect was quadratic for N, K and Mg accumulation. The total nutrient accumulation descending order was: K>N>Ca>S>P>Mg, and considering only the seeds was: N>S>K>P>Ca>Mg, S being highlighted as the second most accumulated nutrient in the seeds. © 2016, Sociedade de Olericultura do Brasil. All rights reserved.

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