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Sakurai, Japan

Iwata T.,Nara Prefectural Mimuro Hospital | Nakahashi K.,Saiseikai Chuwa Hospital | Inoue S.,Nara Medical University | Furuya H.,Nara Medical University
Saudi Journal of Anaesthesia | Year: 2013

Background: Ropivacaine, a long-acting local anesthetic agent, has been used for postoperative analgesia in brachial plexus block (BPB) at high doses. However, use of lower doses would reduce the occurrence of adverse effects. Methods: We applied BPB with low-dose ropivacaine (10 mL of 0.375% ropivacaine) after induction of general anesthesia for surgery of the upper extremities in 62 patients at our hospital. Ropivacaine was administered via a fluoroscopy-guided supraclavicular method. Analgesic effects during surgery, visual analog scale pain scores, skin sensation, muscle strength, and postoperative patient satisfaction indices were evaluated. Results: Fifty-six patients (90.3%) did not require supplemental analgesics during surgery. The remaining six patients were administered fentanyl due to the insufficient analgesic effects of the nerve block. Some adverse effects, including numbness and delayed motor and sensory recovery of the upper extremities, were observed. The mean postoperative patient-evaluated visual satisfaction scale was 94.1. Conclusions: Our results suggest that low-dose ropivacaine is clinically acceptable for BPB under general anesthesia. Source

Shimamoto T.,Wakayama Medical University | Tani M.,Wakayama Medical University | Kawai M.,Wakayama Medical University | Hirono S.,Wakayama Medical University | And 7 more authors.
Pancreas | Year: 2010

Objectives: Some intraductal papillary mucinous neoplasms (IPMNs) have no proliferation to malignant IPMNs, and benign IPMNs can observe the natural course without a surgical intervention. Therefore, an accurate assessment is required to determine the appropriate decision on managing malignant IPMNs. Methods: Quantitative real-time reverse transcription-polymerase chain reaction was performed for pancreatic juice by a LightCycler instrument focused on carcinoembryonic antigen, MUC1, and human telomerase reverse transcriptase. Results: MUC1/glyceraldehyde-3-phosphate dehydrogenase messenger RNA (mRNA) ratio in intraductal papillary mucinous carcinoma (IPMC; median, 4710.7) was significantly higher in intraductal papillary mucinous adenoma (IPMA; median, 727; P = 0.0229). Furthermore, the MUC1/glyceraldehyde-3-phosphate dehydrogenase mRNA ratio in carcinoma in situ and minimum invasive IPMC (median, 26,490) was significantly higher than that in IPMA (P = 0.0152). The cutoff level of MUC1 ratio was determined as 1600 for the division of IPMC from IPMA by the receiver-operating characteristic curve. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of MUC1 mRNA were 88.9%, 71.4%, 80.0%, 83.3%, and 81.3%, respectively. Conclusions: Quantitative reverse transcription-polymerase chain reaction using MUC1 is useful for the detection of malignant IPMN in pure pancreatic juice. Copyright © 2010 by Lippincott Williams & Wilkins. Source

Kitahashi T.,Kyoto Prefectural University | Ikawa S.,Kyoto Prefectural University | Sakamoto A.,Kyoto Prefectural University | Nomura Y.,Tokyo University of Agriculture and Technology | And 7 more authors.
Journal of Agricultural and Food Chemistry | Year: 2012

A water extract of shark cartilage was fractionated into acidic and basic fractions by preparative isoelectric focusing on the basis of the amphoteric nature of samples. The acidic fraction was further fractionated into ethanol-soluble and -precipitate fractions. After the carcinogenesis treatment using N-nitrosobis(2-oxopropyl)amine, hamsters received a diet containing each fraction or purified chondroichin sulfate to give 0.4% (w/w) for 50 days. Only administration of the acidic ethanol-precipitate-fraction-containing diet significantly increased serum inhibitory activity against matrix metalloproteinase (MMP)-9 and reduced the number of adenocarcinomas in the pancreatic duct. The active fraction predominantly consisted of chondroichin sulfate-containing proteoglycan. However, the purified chondroichin sulfate had no significant activity. These results suggest that the protein moiety of the proteoglycan might be involved in the increase of serum inhibitory activity against MMP-9 and suppression of pancreatic carcinogenesis in hamster. © 2011 American Chemical Society. Source

Suzuki Y.,Juntendo University | Suzuki Y.,Nisshin Pharma Inc | Asano M.,Mukogawa Womens University | Sato K.,Kyoto Prefectural University | And 4 more authors.
Biomedical Research | Year: 2011

Wheat gluten hydrolysate (WGH) has been reported to mitigate chronic hepatitis in some patients. We aimed to reproduce and examine this phenomenon in a rat experimental model. Male Sprague-Dawley rats were injected with carbon tetrachloride twice a week for 25 weeks, and 3 weeks later WGH was added to the feed (none, 4%, and 8%) for the remaining 22 weeks. Transition of serum transaminases showed a temporary decrease in aspartate aminotransferase (AST) and a delay in the peaking of alanine aminotransferase (ALT) in the WGH groups. Macroscopically, at the end of the 25 weeks, the progress of cirrhosis was milder in the WGH groups, as indicated by less ascites fluid and the fewer tubercle formations. Histological and immunohistochemical analyses of the liver revealed fewer collagen fibers and less α-smooth muscle actin (SMA) in the 8% WGH group. The ethanol-soluble extract of liver tissue showed plasmin inhibiting activity in the 8% WGH group. The observed modification of the transition of serum transaminases during chronic CCl 4 challenge supports the possibility that WGH mitigates chronic hepatitis, and the liver manifestations after 25 weeks of CCl 4 treatment indicate that WGH ingestion alters the progress of cirrhosis. Source

Kato A.,Nagoya City University | Naiki-Ito A.,Nagoya City University | Nakazawa T.,Nagoya City University | Hayashi K.,Nagoya City University | And 16 more authors.
Oncotarget | Year: 2015

Despite progress in clinical cancer medicine in multiple fields, the prognosis of pancreatic cancer has remained dismal. Recently, chemopreventive strategies using phytochemicals have gained considerable attention as an alternative in the management of cancer. The present study aimed to evaluate the chemopreventive effects of resveratrol (RV) and apocynin (AC) in N-Nitrosobis(2-oxopropyl)amine-induced pancreatic carcinogenesis in hamster. RV- and AC-treated hamsters showed significant reduction in the incidence of pancreatic cancer with a decrease in Ki-67 labeling index in dysplastic lesions. RV and AC suppressed cell proliferation of human and hamster pancreatic cancer cells by inhibiting the G1 phase of the cell cycle with cyclin D1 downregulation and inactivation of AKT-GSK3β and ERK1/2 signaling. Further, decreased levels of GSK3βSer9 and ERK1/2 phosphorylation and cyclin D1 expression in the nuclear fraction were observed in cells treated with RV or AC. Nuclear expression of phosphorylated GSK3βSer9 was also decreased in dysplastic lesions and adenocarcinomas of hamsters treated with RV or AC in vivo. These results suggest that RV and AC reduce phosphorylated GSK3βSer9 and ERK1/2 in the nucleus, resulting in inhibition of the AKT-GSK3β and ERK1/2 signaling pathways and cell cycle arrest in vitro and in vivo. Taken together, the present study indicates that RV and AC have potential as chemopreventive agents for pancreatic cancer. Source

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