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Sankaranarayanan S.,Sairam Advanced Center for Research | Bama P.,Sri Sairam Sidhha Medical College and Research Center | Sathyabama S.,Stella Maris College | Bhuvaneswari N.,Gloris Biomedical Research Center d
Asian Journal of Pharmaceutical and Clinical Research | Year: 2013

Tridax procumbens is a semi prostate annual or short lived perennial herb. The wide spread nature and contribution of the plant in medicine has been identified. The phytochemicals in dried leaves of T. procumbens has been investigated. T. procumbens compounds were tested for cytotoxicity against human lung cancer by MTT assay. The compounf of Rf value 0.66 showed 90% reduced cell viability. NMR, MS and IR spectra revealed the compound as Lupeol. The anticancer potential of the Lupeol against human lung cancer has been evaluated by colonogenic survival determination, cell cycle control, Cell based assay for inhibition of COX-2 activity and DNA fragmentation analysis, an amount of 320 μg/ml concentration of Lupeol compound exhibited potential anticancer property.


Thanigavelan V.,Sairam Advanced Center for Research | Thanigavelan V.,Sri Sairam Siddha Medical College and Research Center | Kaliyamurthi V.,Sairam Advanced Center for Research | Lakshmanakumar V.,Sri Sairam Siddha Medical College and Research Center | And 2 more authors.
Journal of Applied Pharmaceutical Science | Year: 2013

Karpoora Cinthamani Mathirai (KCM) is a traditional Siddha medicinal preparation using to treat Arthritis associated with fever narrated in the text Anubhoga Vaithiya Navaneetham. This formulation has the detoxified ingredients such as Hydrargyrum subchloride and Croton tiglium seeds. The aim was to establish a fingerprint to ensure the quality and safety of KCM. Physicochemical characterization of KCM was carried out using qualitative biochemical analysis and modern techniques such as Fourier transform infra-red spectroscopy, inductively coupled plasma analysis and scanning electron microscopy. Physical evaluation revealed that KCM is a light green colour pill, neutral nature and having solubility in water and HCl with stabilized particle size distribution of 3μ. A clearly identifiable fraction of KCM particles were below 50 nm. The presence of nano sized particles and functional groups carboxylic acids and nitrocompounds in KCM might impart the therapeutic property. Trace elemental analysis of KCM revealed that heavy metals like arsenic, cadmium, mercury and lead were below the deduction limit. Further, elemental analysis of KCM revealed the presence of minerals like calcium, iron, potassium, sodium, and phosphorus under acceptable limits at the prescribed dose of KCM.


Thanigavelan V.,Sairam Advanced Center for Research | Kaliyamurthi V.,Sairam Advanced Center for Research | Pitchiah Kumar M.,Government Siddha Medical College | Elansekaran S.,National Institute of Siddha | Victor Rajamanickam G.,Sairam Advanced Center for Research
Journal of Applied Pharmaceutical Science | Year: 2012

Handa et al., 1986 noted that in India, more than 87 plants are used in 33 patented and proprietary multi-ingredient plant formulations for liver protection. Siddha Materia Medica illustrates a safer, cheap and potential hepatoprotective polyherbal decoction 'Pidangunaari kudineer' comprised of three herbal ingredients such as leaves of Premna tomentosa, pericarp of ripe fruits of Terminalia chebula, rhizomes of Curcuma longa. This formulation is very effective against hepatitis in the clinical practice. The goal of this systematic review of the literature was to summarize the literature on the safety and hepatoprotective activity of above herbs. A manual search of bibliographies of papers identified through an Internet search using multiple search engines. Textbooks on herbal medicine and their bibliographies were also searched. Result and Conclusion: A large number of in vitro, in vivo, human clinical studies on those herbs were identified. These included studies on the antioxidant, anti-inflammatory, antihepatoxicity induced by acetaminophen and antituberculosis drugs, anticarcinogenicity, antimicrobial properties and also phytocompounds were identified to justify the safety and hepatoprotective efficacy. Ethnic background of these herbs results liver protection make Pidangunaari Kudineer for further screening for hepatoprotection.


Geetha V.,Government Siddha Medical College | Elansekaran S.,National Institute of Siddha | Thanigavelan V.,Sairam Advanced Center for Research | Kaliyamurthi V.,Sairam Advanced Center for Research | And 2 more authors.
International Journal of Pharma and Bio Sciences | Year: 2015

Singinatha Choornam, a Siddha polyherbal formulation has been prescribed for Allergic Rhinosinusitis in the literature. The objective was to evaluate the antibacterial activity and safety of Singinatha Choornam in a rat model. Initially, Physico chemical characterization studies were done adopting a methodology of Pharmacopoeial Laboratory for Indian Medicine. Antibacterial study was done by Kirby-Bauer disc diffusion method against gram positive and negative strains comparing with Ciprofloxacin. An Acute toxicity study was done on female wistar albino rat under OECD guidelines 423. Physicochemical parameters results can be used as standard in future for maintaining quality of this drug. Staphylococcus aureus and Escherchia coli were susceptible to 50 μl/disc of aqueous extract of the test drug prepared with 5 g of sample in 50 ml of distilled water. Acute oral toxicity study of Singinatha Choornam revealed no mortality even at the dosage of 2000 mg/kg body weight justifies its recommended therapeutic dosage of 1 g.


Thanigavelan V.,Sairam Advanced Center for Research | Lakshmanakumar V.,Sairam Advanced Center for Research | Kaliyamurthi V.,Sairam Advanced Center for Research | Pitchiah Kumar M.,Government Siddha Medical College | Victor Rajamanickam G.,Sairam Advanced Center for Research
Journal of Applied Pharmaceutical Science | Year: 2012

Sphaeranthus amaranthoides Burm belonging to the family Asteraceae is a rejuvenator herb of Siddha system having Tamil name 'Sivakaranthai'. The present studies deals with a detailed pharmacological including pharmacognostical study particularly on leaf and infloresence of Sphaeranthus amaranthoides. The morphological characters of leaf and inflorescence observed by double staining. It revealed the stomatal index of lower surface with 42-48 and upper su rface with 30-36/mm and presence of leaf resin canals. Inflorescences are cone shap ed and its axis is siphonate. Sivakaranthai leaf powder (SLP) subjected to quality control t est and by phytoconstituents estimation, appreciable presence of calcium, ferrous, tannin, proteins and phenols known. The results of elemental concentration level indicated the presence of toxic metals within the tolerance level. In vitro antibacterial activity evaluation confirmed the good anti-microbial activity at the dilution of 50 |il/disc against bacterial strain such as Streptococcus mutans, Staphylococcus aureus, Escherchia coli Klebsiella pneumonia, Pseudomonas aeruginosa. Rats found safe up to a maximum dose of 2000mg/kg body weight in acute toxicity study following OECD 423 guidelines. The analgesic and anti-inflammation activities evaluation were done by tail flick hot water immersion method and by C arrageenan induced acute hind paw oedema method on Wistar albino rats, respectively. Th e SLP has shown very weak analgesic and moderate anti-inflammatory action rather than standard Indomethacin and no adverse effects produced. In the light of above results, it concluded that Sphaeranthus amaranthoides might use in any suitable formulation in which anti-microbial property and micronutrients needed.


Vembu T.,Tamil University | Vembu T.,Sairam Advanced Center for Research | Sivagnanam M.,Sri Sairam Siddha Medical College and Research Center | Murugan P.K.,Government Siddha Medical College
Journal of Applied Pharmaceutical Science | Year: 2016

Linga Mathirai (LM) is a Siddha Sastric formulation indicated for treating cardiovascular diseases. The present study aimed to evaluate the efficacy of LM against the formation of thrombus and platelet aggregation on compared with Low molecular weight Heparin. In vitro clot lysis and platelet aggregation assays were employed for the study on blood samples of rat. Whole blood samples and platelets obtained by centrifugation were used for clot lysis and aggregation assay respectively. Platelet aggregation was induced by agonists Thrombin and Adenosine diphosphate (ADP). The different concentrations of LM (100, 200, 500, 1000 μL) were prepared with the adjuvant Ginger decoction for test samples and 100 μL low molecular weight heparin (LMWH) was used for treating standard group. Against clot formation and platelet aggregation, LM did not exhibit significant efficacy on compared with normal saline (control) and heparin. However, LM at its higher concentration 1000 μL exhibited significant inhibitory activity on platelet aggregation induced by both Thrombin and ADP when compared with distilled water (control). Under this study, it is concluded that the test drug Linga Mathirai had comparable and slightly lesser efficacy than low molecular weight heparin. © 2016 Thanigavelan Vembu et al.


Kaliyamurthi V.,CSIR - Central Leather Research Institute | Kaliyamurthi V.,Sairam Advanced Center for Research | Thanigavelan V.,Sairam Advanced Center for Research | Victor Rajamanickam G.,Sairam Advanced Center for Research
Journal of Biosciences | Year: 2012

A central hypothesis in the study of Alzheimer's disease (AD) is the accumulation and aggregation of β-amyloid peptide (Aβ). Recent epidemiological studies suggest that patients with elevated cholesterol and decreased estrogen levels are more susceptible to AD through Aβ accumulation. To test the above hypothesis, we used ovariectomized with diet-induced hypercholesterolemia (OVX) and hypercholesterolemia (HCL) diet alone mouse models. HPLC analysis reveals the presence of beta amyloid in the OVX and HCL mice brain. Congo red staining analysis revealed the extent of amyloid deposition in OVX and hypercholesterolemia mice brain. Overall, Aβ levels were higher in OVX mice than in HCL. Secondly, estrogen receptors α (ERα) were assessed by immunohistochemistry and this suggested that there was a decreased expression of ER α in OVX animals when compared to hypercholesterolemic animals. Aβ was quantified by Western blot and ELISA analysis. Overall, Aβ levels were higher in OVX mice than in HCL mice. Our experimental results suggested that OVX animals were more susceptible to AD with significant increase in Aβ peptide. © Indian Academy of Sciences.

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