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Bezborodkina N.N.,Russian Academy of Sciences | Chestnova A.Y.,Russian Academy of Sciences | Okovity S.V.,Saint Petersburg State Chemical Pharmaceutical Academy | Kudryavtsev B.N.,Russian Academy of Sciences
Experimental and Toxicologic Pathology | Year: 2014

Cirrhotic patients often demonstrate glucose intolerance, one of the possible causes being a decreased glycogen-synthesizing capacity of the liver. At the same time, information about the rates of glycogen synthesis in the cirrhotic liver is scanty and contradictory. We studied the dynamics of glycogen accumulation and the activity of glycogen synthase (GS) and glycogen phosphorylase (GP) in the course of 120min after per os administration of glucose or fructose to fasted rats with CCl4-cirrhosis or fasted normal rats. Blood serum and liver pieces were sampled for examinations. In the normal rat liver administration of glucose/fructose initiated a fast accumulation of glycogen, while in the cirrhotic liver glycogen was accumulated with a 20min delay and at a lower rate. In the normal liver GS activity rose sharply and GPa activity dropped in the beginning of glycogen synthesis, but 60min later a high synthesis rate was sustained at the background of a high GS and GPa activity. Contrariwise, in the cirrhotic liver glycogen was accumulated at the background of a decreased GS activity and a low GPa activity. Refeeding with fructose resulted in a faster increase in the GS activity in both the normal and the cirrhotic liver than refeeding with glucose. To conclude, the rate of glycogen synthesis in the cirrhotic liver is lower than in the normal one, the difference being probably associated with a low GS activity. © 2013 Elsevier GmbH. Source


Bezborodkina N.N.,Russian Academy of Sciences | Okovity S.V.,Saint Petersburg State Chemical Pharmaceutical Academy | Chestnova A.Y.,Russian Academy of Sciences | Kudryavtsev B.N.,Russian Academy of Sciences
Hepatology International | Year: 2013

Purpose: To investigate the accumulation of glycogen in cirrhotic rat liver at several time intervals after per os administration of glucose to fasted animals. Methods: Liver cirrhosis was produced by inhalation of the hepatotropic poison CCl4. Glycogen concentration in the liver was determined biochemically. Glycogen content in hepatocytes was measured cytofluorimetrically in the smears stained with a fluorescent PAS reaction. Glycogen content in the hepatocytes of the portal and the central zone of the liver lobule was determined by absorption cytophotometry. Results: Rats poisoned with CCl 4 for 6 months developed typical liver cirrhosis characterized by a fourfold (p < 0.001) increase in the proportion of the connective tissue. In the cirrhotic rats fasted for 48 h, glycogen concentration in the liver and glycogen content in hepatocytes were lower as compared with the control by 36 and 27 % (p < 0.01), respectively. According to data obtained by different methods, the control animals accumulated glycogen at a high rate. In particular, the glycogen content in hepatocytes increased by 34 % after 10 min (p < 0.01). In the cirrhotic rats, glycogen content remained at the same level for 20 min. In both groups of animals, hepatocytes of the portal zone accumulated more glycogen than those of the central zone. Conclusions: Glycogen accumulation in cirrhotic rats starts after a delay and proceeds at a lower rate than in the norm. © 2013 Asian Pacific Association for the Study of the Liver. Source


Zaytsev A.A.,N.N. Burdenko Main Military Clinical Hospital | Okovityi S.V.,Saint Petersburg State Chemical Pharmaceutical Academy
Terapevticheskii Arkhiv | Year: 2014

This publication deals with topical problems in the management of patients with cough. It presents its epidemiology and clinical classification, an analysis of its causes, a list of required diagnostic techniques, and areas of pharmacotherapy. Emphasis is laid on the differential diagnosis of different abnormalities and diseases, the leading clinical sign of which is cough. The authors provide the detailed characteristics of medicaments for its treatment and the principles of rational antitussive and mucoactive pharmacotherapy. Source


Falkova M.T.,Saint Petersburg State University | Bulatov A.V.,Saint Petersburg State University | Pushina M.O.,Saint Petersburg State University | Ekimov A.A.,Saint Petersburg State Chemical Pharmaceutical Academy | And 2 more authors.
Talanta | Year: 2014

An automation of the extraction of analytes from solid samples into the aqueous phase based on multicommutated stepwise injection analysis concept has been suggested. The feasibility of the approach has been demonstrated by determination of ascorbic acid as model analyte. The method includes automated extraction of ascorbic acid from solid sample into borate buffer solution pH 8 in mixing chamber during vigorous mixing by nitrogen stream, and subsequent detection by capillary zone electrophoresis at 254 nm. The method has a linear range of 0.1-5.0 mg g-1 for ascorbic acid with the LOD of 0.03 mg g-1. The sample throughput was 7 h-1. This method was applied for determination of ascorbic acid in various medicinal plants and food samples. © 2014 Elsevier B.V. All rights reserved. Source


Falkova M.T.,Saint Petersburg State University | Pushina M.O.,Saint Petersburg State University | Bulatov A.V.,Saint Petersburg State University | Alekseeva G.M.,Saint Petersburg State Chemical Pharmaceutical Academy | Moskvin L.N.,Saint Petersburg State University
Analytical Letters | Year: 2014

A simple, rapid, and automated method for the spectrophotometric determination of flavonoids in medicinal plants was developed using a stepwise injection manifold. The determination was based on formation of colored complexes of flavonoids with Al(III) in micellar media. Analytical characteristics of the determination were significantly improved when cetylpyridinium chloride was used as a micellar catalyst. It was found that the rate of colored complex formation of rutin with Al(III) increased (k = (1.8 ± 0.1) · 104 min-1mol-2 L2) in the presence of cetylpyridinium chloride. Under the optimum conditions, a linear response was found from 0.004 to 0.2% (volume of weight %). The detection limit (3s) was determined as 0.001% rutin versus the weight of the sample. The developed method was used for the analysis of different medicinal plants for flavonoids. © 2014 Copyright Taylor & Francis Group, LLC. Source

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