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Stel'makh V.V.,North State Medical University | Trashkov A.P.,RAS Sechenov Institute of Evolutionary Physiology and Biochemistry | Kovalenko A.L.,Federal Medico Biological Agency | Zaplutanov V.A.,Saint Petersburg State Chemical Pharmaceutical Academy
Eksperimental'naya i Klinicheskaya Farmakologiya | Year: 2016

We present results of preclinical evaluation of the specific efficiency of new metabolic corrector runikhol on models of non-alcoholic fatty liver disease in laboratory rats (228 albino males of gray Wistar rats weighing 220-240 g). The drug based on succinic acid positively influences the key types of metabolism impaired under conditions of development of the metabolic syndrome. Results of research testify to high efficiency and good prospects of using runikhol in the treatment of metabolic syndrome accompanied by organ disorders.


Falkova M.T.,Saint Petersburg State University | Pushina M.O.,Saint Petersburg State University | Bulatov A.V.,Saint Petersburg State University | Alekseeva G.M.,Saint Petersburg State Chemical Pharmaceutical Academy | Moskvin L.N.,Saint Petersburg State University
Analytical Letters | Year: 2014

A simple, rapid, and automated method for the spectrophotometric determination of flavonoids in medicinal plants was developed using a stepwise injection manifold. The determination was based on formation of colored complexes of flavonoids with Al(III) in micellar media. Analytical characteristics of the determination were significantly improved when cetylpyridinium chloride was used as a micellar catalyst. It was found that the rate of colored complex formation of rutin with Al(III) increased (k = (1.8 ± 0.1) · 104 min-1mol-2 L2) in the presence of cetylpyridinium chloride. Under the optimum conditions, a linear response was found from 0.004 to 0.2% (volume of weight %). The detection limit (3s) was determined as 0.001% rutin versus the weight of the sample. The developed method was used for the analysis of different medicinal plants for flavonoids. © 2014 Copyright Taylor & Francis Group, LLC.


Zaytsev A.A.,Nn Burdenko Main Military Clinical Hospital | Okovityi S.V.,Saint Petersburg State Chemical Pharmaceutical Academy
Terapevticheskii Arkhiv | Year: 2014

This publication deals with topical problems in the management of patients with cough. It presents its epidemiology and clinical classification, an analysis of its causes, a list of required diagnostic techniques, and areas of pharmacotherapy. Emphasis is laid on the differential diagnosis of different abnormalities and diseases, the leading clinical sign of which is cough. The authors provide the detailed characteristics of medicaments for its treatment and the principles of rational antitussive and mucoactive pharmacotherapy.


Kozhemyakina N.V.,Saint Petersburg State Chemical Pharmaceutical Academy | Ananyeva E.P.,Saint Petersburg State Chemical Pharmaceutical Academy | Gurina S.V.,Saint Petersburg State Chemical Pharmaceutical Academy | Galynkin V.A.,Saint Petersburg State Chemical Pharmaceutical Academy
Applied Biochemistry and Microbiology | Year: 2010

A study is made on a strain of higher basydiomycete Flammulia velutipes (Fr.) P. Karst. The conditions of maximum biomass production by Flammulia velutipes were studied. Soluble and insoluble fractions were isolated from mycelium. The composition of cultured mycelium and aqueous extracts from mycelium were investigated. These objects mainly contained carbohydrates (65.3 and 84.0% in insoluble and soluble fractions, respectively, and 56% mycelium), proteins (7.5-10.0% in fractions and 17.5% in mycelium), as well as an insignificant amount of mineral substances. The main carbohydrate component of fractions was glucose (53.6-78.8%); galactose and mannose were also present, as well as fucose and xylose in insignificant amounts. The aqueous extracts from mycelium demonstrated immunomodulating activity. They rendered a stimulating effect on the functional activity of macrophages-central cells of the reticluoendothelial system. The soluble fraction had a more pronounced effect than the insoluble fraction. © 2010 Pleiades Publishing, Ltd.


Bezborodkina N.N.,Russian Academy of Sciences | Chestnova A.Y.,Russian Academy of Sciences | Okovity S.V.,Saint Petersburg State Chemical Pharmaceutical Academy | Kudryavtsev B.N.,Russian Academy of Sciences
Experimental and Toxicologic Pathology | Year: 2014

Cirrhotic patients often demonstrate glucose intolerance, one of the possible causes being a decreased glycogen-synthesizing capacity of the liver. At the same time, information about the rates of glycogen synthesis in the cirrhotic liver is scanty and contradictory. We studied the dynamics of glycogen accumulation and the activity of glycogen synthase (GS) and glycogen phosphorylase (GP) in the course of 120min after per os administration of glucose or fructose to fasted rats with CCl4-cirrhosis or fasted normal rats. Blood serum and liver pieces were sampled for examinations. In the normal rat liver administration of glucose/fructose initiated a fast accumulation of glycogen, while in the cirrhotic liver glycogen was accumulated with a 20min delay and at a lower rate. In the normal liver GS activity rose sharply and GPa activity dropped in the beginning of glycogen synthesis, but 60min later a high synthesis rate was sustained at the background of a high GS and GPa activity. Contrariwise, in the cirrhotic liver glycogen was accumulated at the background of a decreased GS activity and a low GPa activity. Refeeding with fructose resulted in a faster increase in the GS activity in both the normal and the cirrhotic liver than refeeding with glucose. To conclude, the rate of glycogen synthesis in the cirrhotic liver is lower than in the normal one, the difference being probably associated with a low GS activity. © 2013 Elsevier GmbH.


Berezin A.S.,Saint Petersburg State Chemical Pharmaceutical Academy | Lomkova E.A.,Saint Petersburg State Chemical Pharmaceutical Academy | Skorik Yu.A.,Saint Petersburg State Chemical Pharmaceutical Academy
Russian Chemical Bulletin | Year: 2012

This review summarizes the current research on the development of biologically active polymer compounds based on chitosan and its derivatives; it systematizes approaches taken for the design of conjugates based on a given polymer, and it covers the latest trends in the development of targeted drug delivery systems. An analysis of published data shows that the conjugation of biologically active substances with chitosan and its derivatives results in promising materials for use as drug delivery systems and for the control of the properties of the biologically active substances. © 2012 Springer Science+Business Media New York.


Rudakova A.V.,Saint Petersburg State Chemical Pharmaceutical Academy | Tatarskiy B.A.,Saint Petersburg State Chemical Pharmaceutical Academy
Kardiologiya | Year: 2014

Background: Atrial fibrillation is associated with development of thromboembolic events. New oral anticoagulants (apixaban, rivaroxaban and dabigatran) are recommended for antithrombotic therapy in patients with non-valvular atrial fibrillation (NVAF) with moderate and high risk of stroke. Objectives: The objective of this study was to evaluate the cost-effectiveness ratio of apixaban compared to dabigatran and rivaroxaban in patients with NVAF from the Russian Federation national health care system perspective. Methods. This analysis used a Markov model that allowed estimation of the incremental cost-effectiveness ratio (ICER) for apixaban compared to rivaroxaban and dabigatran 110 mg and 150 mg over lifetime horizon for patients with NVAF. The model enclosed cardiovascular event rates based on the results of the indirect treatment comparison that combined data from the randomized clinical trials comparing clinical effectiveness and safety of apixaban, rivaroxaban and dabigatran with warfarin (ARISTOTLE, ROCKET-AF, RE-LY). The following cardiovascular events were considered: ischemic and hemorrhagic stroke, systemic embolism, intracranial hemorrhage, other major bleeds, clinically relevant non-major bleeds and myocardial infarction. Direct medical costs were determined based on the rates of the compulsory national medical insurance system. The price of the new oral anticoagulants was taken as a weighted average tender price for the year 2013. In the model both costs and benefits (qualityadjusted life years and life-years) were discounted at 3.5%. Cost-effectiveness threshold was set at 1.4 million rubles per quality-adjusted life year (QALY) gained and corresponded to the three times GDP per capita in 2013 in the Russian Federation. Results. In the base case analysis it was demonstrated that apixaban compared to dabigatran 110 mg and 150 mg and rivaroxaban provided additional 0.101, 0.060 and 0.072 life years as well as additional 0.063; 0.038 and 0.041 QALYs respectively. Over lifetime horizon apixaban compared to dabigatran 110 mg and 150 mg and rivaroxaban required additional treatment costs equal to 22.78; 31.18 and 6.70 thousands rubles, respectively. With that estimated incremental cost-effectiveness ratio for apixaban compared to dabigatran 110 mg and 150 mg and rivaroxaban was 362.60, 805.54 and 162.45 thousands rubles per QALY correspondingly. Conclusion. Apixaban provided increased life expectancy compared to other new anticoagulants and may be considered as a cost-effective alternative to dabigatran 110 mg and 150 mg and rivaroxaban from the Russian Federation national health care system perspective.


Fedorova E.V.,Saint Petersburg State Chemical Pharmaceutical Academy | Buryakina A.V.,Saint Petersburg State Chemical Pharmaceutical Academy | Vorobieva N.M.,Saint Petersburg State Chemical Pharmaceutical Academy | Baranova N.I.,Saint Petersburg State Chemical Pharmaceutical Academy
Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry | Year: 2013

The review considers the biological role of vanadium, its involvement in various processes in humans and other mammals, and the anti-diabetic effect of its compounds. Vanadium salts have persistent hypoglycemic and antihyperlipidemic effects and reduce the probability of secondary complications in animals with experimental diabetes. The review contains detailed description of all major synthesized vanadium complexes with antidiabetic activity. Currently, vanadium complexes with organic ligands are more effective and safer than the inorganic salts. Despite well-documented efficacy of these compounds as the anti-diabetic agents in animal models, only one organic complex of vanadium is currently under the second phase of clinical trials. All of the considered data suggest that vanadium compounds are a new promising class of drugs in modern pharmacotherapy of diabetes. © 2013 Pleiades Publishing, Ltd.


PubMed | Russian Academy of Sciences and Saint Petersburg State Chemical Pharmaceutical Academy
Type: Journal Article | Journal: Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie | Year: 2014

Cirrhotic patients often demonstrate glucose intolerance, one of the possible causes being a decreased glycogen-synthesizing capacity of the liver. At the same time, information about the rates of glycogen synthesis in the cirrhotic liver is scanty and contradictory. We studied the dynamics of glycogen accumulation and the activity of glycogen synthase (GS) and glycogen phosphorylase (GP) in the course of 120min after per os administration of glucose or fructose to fasted rats with CCl4-cirrhosis or fasted normal rats. Blood serum and liver pieces were sampled for examinations. In the normal rat liver administration of glucose/fructose initiated a fast accumulation of glycogen, while in the cirrhotic liver glycogen was accumulated with a 20min delay and at a lower rate. In the normal liver GS activity rose sharply and GPa activity dropped in the beginning of glycogen synthesis, but 60min later a high synthesis rate was sustained at the background of a high GS and GPa activity. Contrariwise, in the cirrhotic liver glycogen was accumulated at the background of a decreased GS activity and a low GPa activity. Refeeding with fructose resulted in a faster increase in the GS activity in both the normal and the cirrhotic liver than refeeding with glucose. To conclude, the rate of glycogen synthesis in the cirrhotic liver is lower than in the normal one, the difference being probably associated with a low GS activity.


PubMed | Ivano Frankivsk National Medical University, Saint Petersburg State Chemical Pharmaceutical Academy and Institute of Toxicology of the Federal Medical and Biological Agency
Type: | Journal: Khirurgiia | Year: 2016

The aim of the study was to assess the effectiveness of cytoflavin inclusion in complex treatment of patients with hypoparathyroidism occurring after thyroid surgery. To do this, an analysis of treatment of 54 patients who received surgical treatment for thyroid diseases of various etiologies. Depending on the scheme of post-operative medical therapy, the patients were divided into 2 groups: group I patients (27 persons) received basic therapy according to regulatory documents, II of the group (27 patients) in addition cytoflavin intravenous drip of 10 ml to 200 ml 0.9% NaCl for 7 days. In addition to standard clinical and laboratory examination, all patients were studied and the level of active ionized Ca2+ and PTH, oxidation products of the modified protein, lipid peroxidation and antioksidazation system dynamics - 1, 3 and 7 days after surgery. It was revealed that in the first days after the surgical treatment of patients significantly reduced the levels of ionized Ca2+ and PTH that may be regarded as signs of postoperative hypoparathyroidism, as well as the activation of the system of lipid peroxidation and antioxidant system depression. Turning cytoflavin in drug regimens improved the efficiency of the treatment, which was manifested in arresting to 7 days of monitoring signs of hypoparathyroidism, improve tissue oxygenation and restoring the balance by reducing the activity of the system of lipid peroxidation and antioxidant system strengthening.

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