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Pozharitskaya O.N.,Interregional Fund Adaptation | Shikov A.N.,Interregional Fund Adaptation | Makarova M.N.,Interregional Fund Adaptation | Kosman V.M.,Interregional Fund Adaptation | And 4 more authors.
Phytomedicine | Year: 2010

The anti-inflammatory potential of three doses of an aqueous infusion of aerial parts Bidens tripartita L. against carrageenan-induced acute paw edema in rats was investigated. A phytochemical study and qualitative-quantitative analyses revealed the presence of flavonoids, tannins, polysaccharides, phenols, amino acids, ascorbic acid, organic acids and polyacetylenes. Infusion doses of 20 ml/kg body wt. exhibited significant anti-inflammatory activity in rats, as compared with indomethacin. In addition, the infusion showed analgesic properties in a hot-plate test and antipyretic properties in carrageenan-induced local hyperthermia, both in rats. The effects were dose-dependent. Our results provide evidence for the potential usefulness of B. tripartita infusion in the treatment of inflammatory disorders. © 2009 Elsevier GmbH. All rights reserved.


Shikov A.N.,Saint Petersburg Institute of Pharmacy | Pozharitskaya O.N.,Saint Petersburg Institute of Pharmacy | Makarova M.N.,Saint Petersburg Institute of Pharmacy | Damien Dorman H.J.,University of Helsinki | And 3 more authors.
Journal of Functional Foods | Year: 2010

The adaptogenic effect of Bergenia crassifolia black and fermented leaves was examined based on the forced swimming capacity and the change of biochemical parameters in mice. The treatment groups were orally administered infusions of black and fermented leaves of 3 populations of B. crassifolia, while the control group received distilled water for 7 days. Infusions from black leaves of B. crassifolia insignificantly enhanced the maximum swimming capacity of mice by increasing fat utilization, and by delaying the accumulation of plasma lactate while infusions from fermented leaves of B. crassifolia significantly enhanced the maximum swimming capacity of mice without change of the body weight by increasing glucose utilization and decreasing lactate level compared to the control group. © 2009 Elsevier Ltd. All rights reserved.


Shikov A.,Saint Petersburg Institute of Pharmacy | Pozharitskaya O.,Saint Petersburg Institute of Pharmacy | Ivanova S.,Saint Petersburg Institute of Pharmacy | Makarov V.,Saint Petersburg Institute of Pharmacy | And 2 more authors.
Journal of Planar Chromatography - Modern TLC | Year: 2010

A selective and simplest possible high-performance TLC (HPTLC) method for quantification of oenothein B on the basis of the free gallic acid and total gallic acid content after acid hydrolysis has been developed and used for analysis of cultivated Epilobium angustifolium. HPTLC of aqueous extracts of E. angustifolium was performed on silica gel with benzene-methanol-acetic acid 90:16:8 (v/v), as mobile phase. Quantitative evaluation of the plate was performed at 570 nm after derivatization with 1% ethanolic FeCl3 solution. The method was validated for precision, repeatability, and accuracy. Average recovery of the active ingredient from the samples was in the range 95.43-104.57%. The calibration plots were linear in the range 440-2200 ng per band. The technique was used, for the first time, for estimation of the oenothein B content of cultivated E. angustifolium. The oenothein B content was highest at budding time (18.4-20.2 mg g-1) and decreased dramatically to 1.0 mg g-1 after flowering. © Akadémiai Kiadó, Budapest.


Shikov A.N.,Saint Petersburg Institute of Pharmacy | Pozharitskaya O.N.,Saint Petersburg Institute of Pharmacy | Makarova M.N.,Saint Petersburg Institute of Pharmacy | Kovaleva M.A.,Saint Petersburg Institute of Pharmacy | And 5 more authors.
Phytomedicine | Year: 2012

The objective of this study was to evaluate the feeding behavior and weight gain in rats with high-calorie diet-induced obesity that are treated with Bergenia crassifolia black and fermented leaves extracts. The daily dietary intake of all treated animals was reduced to 40% compared with the control group on day 22 of the experiment. A significant improvement in glucose tolerance was noted after 7 days of treatment with the Bergenia extracts. In rats treated with an extract of black leaves for 7 days, a significant reduction in the serum triglyceride level, 45% (p < 0.05), compared with the control group was observed. However, the treatment did not affect the cholesterol level. Our results provide evidence for the potential use of B. crassifolia as an appetite and energy intake suppressant. © 2012 Elsevier GmbH.


Fan T.-P.,University of Cambridge | Deal G.,Global Regulatory Services | Koo H.-L.,River Cam International | Rees D.,Salupont Consulting | And 14 more authors.
Journal of Ethnopharmacology | Year: 2012

Ethnopharmacological relevance: GP-TCM is the first EU-funded Coordination Action consortium dedicated to traditional Chinese medicine (TCM) research. One of the key deliverables of the Work Package 7 in GP-TCM was to investigate information of the existing requirements for registration of TCM products listed by global regulatory bodies. The paper aims to collate data and draw comparison of these regulations. Case studies are also presented to illustrate the problems involved in registering TCM products in different regions worldwide. Materials and methods: A collaborative network task force was established during the early stage of the GP-TCM project and operated through exchanges, teleconferences and focused discussions at annual meetings. The task force involved coordinators, academics who are actively involved with R&D of Chinese herbal medicines, experts on monographic standards of Chinese materia medica, representatives from regulatory agencies, experts from industries in marketing Chinese medicines/herbal medicines and natural products. The co-ordinators took turns to chair teleconferences, led discussions on specific issues at AGM discussion sessions, at joint workshops with other work-packages such as WP1 (quality issues), WP3 (toxicology issues) and WP6 (clinical trial issues). Collectively the authors were responsible for collating discussion outcomes and updating written information. Results: A global overview of regulations on herbal registration has been compiled during the three years of the consortium. The regulatory requirements for registration of herbal products in the EU and China were compared, and this is extended to other regions/countries: Africa, Australia, Brazil, Canada, Japan, Russia, South Korea, Taiwan, and the United States. A wide variation of the regulations for the categories of herbal products exists: food (functional food, novel foods, dietary food for special medical purpose, foods for particular nutritional use, food supplement); cosmetic, traditional herbal medicine products; herbal medicines for human use and veterinary use. Conclusion: The regulatory issues for registration of herbal products are complicated among the countries and regions worldwide. The information summarised in the text is for reference only. Some regulations which are presented in this review are still in legislation process and may change in due course. Before taking any regulatory action, readers are advised to consult current official legislation and guidance and/or to seek appropriate professional advice. The lessons learnt from global regulation of TCM will provide valuable insights for regulation of other traditional medicine such as Ayurveda and Unani medicine, as well as other forms of indigenous medicine. The WHO is well placed to co-ordinate a consultation process with the aim of putting forward suggestions for harmonisation to key regulatory agencies. © 2012 Elsevier Ireland Ltd. All rights reserved.

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