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Tai C.-J.,Taipei Medical University Hospital | Tai C.-J.,Taipei Medical University | Cheng C.-W.,Taipei Medical University | Su H.-Y.,Saint Marys Hospital Luodong | And 8 more authors.
Tumor Biology | Year: 2014

Thrombomodulin (TM) has been shown to regulate many physiological and pathological processes, including inflammation, thrombosis, and tumor progression. TM is also a natural anticoagulant that maintains circulatory homeostasis in endothelial cells. However, little is known regarding the role of TM in the progression and metastasis of cervical cancer. TM-specific RNA interference and a cDNA expression vector were used to manipulate TM expression in cervical cancer cells. Cell growth and cell migration were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, transwell migration assays, and a biosensor system. TM silencing did not affect the growth rate of the cells. However, cell migration was dramatically enhanced after silencing of TM in HeLa cells. The overexpression of TM in cervical cancer cells only slightly influenced their proliferative capacity. After overexpression of TM in HeLa cells, their migratory capability was suppressed. Furthermore, we found that the decreased expression of E-cadherin and increase of zeb-1 and snail expression in TM-silenced cells which may be correlated with the results of knocking-down TM increases the migratory ability in this study. Our results demonstrate that TM may slightly regulate the growth but played the important role in the migratory ability of cervical cancer cells, suggesting that TM could potentially serve as a novel prognostic and therapeutic target in cervical cancer. © 2013 International Society of Oncology and BioMarkers (ISOBM).


Shiao C.-C.,Saint Marys Hospital Luodong | Shiao C.-C.,Saint Marys College | Wu P.-C.,Mackay Memorial Hospital | Huang T.-M.,National Taiwan University Hospital | And 8 more authors.
Critical Care | Year: 2015

Acute kidney injury (AKI) has been a global health epidemic problem with soaring incidence, increased long-term risks for multiple comorbidities and mortality, as well as elevated medical costs. Despite the improvement of patient outcomes following the advancements in preventive and therapeutic strategies, the mortality rates among critically ill patients with AKI remain as high as 40-60 %. The distant organ injury, a direct consequence of deleterious systemic effects, following AKI is an important explanation for this phenomenon. To date, most evidence of remote organ injury in AKI is obtained from animal models. Whereas the observations in humans are from a limited number of participants in a relatively short follow-up period, or just focusing on the cytokine levels rather than clinical solid outcomes. The remote organ injury is caused with four underlying mechanisms: (1) "classical" pattern of acute uremic state; (2) inflammatory nature of the injured kidneys; (3) modulating effect of AKI of the underlying disease process; and (4) healthcare dilemma. While cytokines/chemokines, leukocyte extravasation, oxidative stress, and certain channel dysregulation are the pathways involving in the remote organ damage. In the current review, we summarized the data from experimental studies to clinical outcome studies in the field of organ crosstalk following AKI. Further, the long-term consequences of distant organ-system, including liver, heart, brain, lung, gut, bone, immune system, and malignancy following AKI with temporary dialysis were reviewed and discussed. © 2015 Shiao et al.


Lee C.-Y.,Saint Marys Hospital Luodong | Hsieh P.-C.,National Taipei University of Nursing and Health Sciences | Su H.-F.,National Taipei University of Nursing and Health Sciences
Taiwan Journal of Public Health | Year: 2013

Objectives: The present study evaluated the relationship between emotional labor and mental health among nurses in Catholic hospitals in Taiwan. Methods: Based on a cross- sectional and correctional study design, the researcher invited nurses from six Catholic hospitals to participate between July 1 and October 30, 2010. Instruments were the Emotional Labor Scale developed by Shang Ping Lin and the Self Reporting Questionnaire 20 (SRQ-20) developed by the World Health Organization. Results: A total of 653 subjects were invited to participate, and 624 completed questionnaires were returned for an effective response rate of 95.56%. By using descriptive statistics, ANOVA, correlation, and regression analysis in SPSS 18.0, we found that: (1) Subjects had a relative higher degree of emotional labor (M ± SD = 5.41 ± 1.19) and poorer mental health status (M ± SD = 2.81 ±1.05) as measured by the SRQ-20; of 50.4; (2) There were significant differences in rules for emotional display, superficial acting, and mental health among different groups based on educational level, position, and working department; (3) Age and service seniority had a highly positive correlation with rules for emotional display, superficial acting, and mental health, whereas age was positively associated with deep acting; (4) Rules for emotional display rule, superficial acting, frequency and duration of interaction, and deep acting were highly correlated with mental health; (5) Emotional labor accounted for 21% of the variance in mental health. Conclusions: Nursing staff bear a high level of emotional labor with unsatisfactory mental health status. Administrators should pay close attention to these issues.


Wei P.-L.,Taipei Medical University Hospital | Wei P.-L.,Graduate Institute of Clinical Medicine | Tu C.-C.,Taipei Medical University Hospital | Tu C.-C.,Graduate Institute of Clinical Medicine | And 10 more authors.
Journal of Agricultural and Food Chemistry | Year: 2013

Shikonin is a traditional Oriental medical herb extracted from Lithospermum erythrorhizon. Many studies have shown that shikonin possesses anticancer ability against many different cancers, including hepatocellular carcinoma (HCC). Recently, tumor metastasis has been become an important clinical obstacle. However, the effect of shikonin on metastasis by HCC is unknown. The 50% inhibitory concentration (IC50) of shikonin on HCC cells was determined by an MTT assay and the xCELLigence biosensor system. The migratory ability of HCC cells was detected by a transwell migration assay and the xCELLigence biosensor system. Matrix metalloproteinase-2 and -9 (MMP-2 and -9) expression levels were determined by Western blotting, and the activities of MMP-2 and -9 were determined by gelatin zymography. We found that IC 50 values of HepJ5 and Mahlavu cells to shikonin treatment were around 2 μM. Exposure to a low dose of shikonin (0-0.4 μM) did not influence the survival of HCC cells. Interestingly, exposure to a low dose of shikonin inhibited the migratory ability on HepJ5 and Mahlavu cells. To further dissect the mechanism, we found that treatment with a low dose of shikonin reduced the activities and expression levels of MMP-2 and -9, which were correlated with the decreased cell migratory ability of HCC cells. In addition, we found a decrease of vimnetin expression, but no influence on the expression levels of N-cadherin, TWIST, or GRP78. In mechanism dissecting, we found that shikonin treatment may suppress the phosphorylation of AKT and then reduce the NF-κB (NF = nuclear factor) levels, but has no influence on the levels of c-Fos and c-Jun. Furthermore, we also found that shikonin may also reduce the phosphorylation of IκB. We concluded that a low dose of shikonin can suppress the migratory ability of HCC cells through downregulation of expression levels of vimentin and MMP-2 and -9. Our findings suggest that shikonin may be a new compound to prevent the migration of HCC cells. © 2013 American Chemical Society.


Huang R.-R.,Kai Suan Psychiatric Hospital | Chen Y.-S.,Saint Marys Hospital Luodong | Chen C.-C.,Kai Suan Psychiatric Hospital | Chou F.H.-C.,Kai Suan Psychiatric Hospital | And 5 more authors.
Psychiatry and Clinical Neurosciences | Year: 2012

Aim: This study explored the associations of personal, disease, family, and social factors with quality of life (QoL) in patients with two common types of chronic mental illness (CMI) living in Kaohsiung City, Taiwan. Methods: Using a convenience sample and a cross-sectional design, 714 patients (50.1% male, 49.9% female) with CMI (72.1% schizophrenia and 27.9% affective disorder) and their caregivers were recruited. Demographic information was collected via the following questionnaires: 12-Item Short-Form Health Survey (SF-12), five-item Brief Symptom Rating Scale (BSRS-5), Caregiver Burden Scale, and Clinical Global Impressions (CGI-S) Scale. Pearson correlations and hierarchical regression analyses were used to predict QoL. Results: Disease factors accounted for 17-50% of the change in variance. Predictors of low mental subscale scores included the following: high psychological distress and high family burden as well as a history of suicide attempts, negative caregiver attitudes, and living away from home. Disease factors also explained the greatest variance in the physical subscales. Predictors of low physical subscale scores included the following: high psychological distress, age, unemployment, a history of suicide attempts, high family burden, and living alone. Conclusions: Disease factors were the most important predictors of QoL in patients with CMI. Family factors were more important than social factors on the mental subscales. Differential relationships were also found for the other two dimensions. Together, these results indicate that a wide range of factors improve the QoL in patients with CMI. © 2012 The Authors. Psychiatry and Clinical Neurosciences © 2012 Japanese Society of Psychiatry and Neurology.

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