Kim M.Y.,Chungnam National University |
Choi T.-Y.,Chungnam National University |
Kim J.-H.,Gyeongsang National University |
Lee J.H.,Chungnam National University |
And 6 more authors.
Journal of Dermatological Science | Year: 2010
Background: Melanocyte dendrites serve as the principal conduit for melanosome transfer. The dendrite formation requires actin polymerization mediated by Rho family GTPases including RhoA, Rac1 and Cdc42. Objective: The aim of this study is to investigate and explore the involvement of p38 MAPK in melanocyte dendrite formation. Methods: We transduced melanoma cells with adenovirus harboring the expression cassette for constitutive active form of MKK6, an upstream MAPKK for p38 MAPK. Results: We investigated the effect of melanogenic inducers on melanocyte dendricity, using SK-mel-24 melanoma cells because that this cell line is refractory to several melanogenic inducers in terms of melanogenesis. TPA-induced the phosphorylation of p38 MAPK and the elongation of dendrite length, suggesting that MKK6 may be involved in this process. Overexpression of the constitutive active form of MKK6 resulted in significant elongation of dendrites in the melanoma cell line SK-mel-24. Moreover, overexpression of MKK6 ultimately led to the upregulation of Cdc42 and Rac1, suggesting that MKK6 acts as a crucial upstream signaling molecule for Rho family GTPases. When overexpressed in normal human epidermal melanocytes, MKK6 led also the increase of dendrite length. Conclusion: These results suggest that MKK6 is an authentic regulator for melanocytes dendricity, through the modulation of Rho family GTPases. © 2010 Japanese Society for Investigative Dermatology.
Kim M.J.,Seoul National University |
Lim C.,Seoul National University |
Lee J.Y.,Saimdang Cosmetics Co. |
Im K.R.,Saimdang Cosmetics Co. |
And 2 more authors.
Journal of Biomolecular Screening | Year: 2013
There is a growing interest in alopecia prevention strategies, as the number of alopecia patients is increasing. We examine the efficacy of herbal medicine for hair growth promotion/hair loss inhibition in two cell lines via Western blot and high-content screening (HCS). Nine herbal extracts were obtained from three different herbal medicine mixtures using 3 different extraction methods. Five target proteins - IGF-1 (insulin-like growth factor-1), TGF-β2 (transforming growth factor-β2), VEGF (vascular endothelial growth factor), DKK-1 (Dickkopf-1), and Wnt5α - were observed for the assessment of hair growth promotion/hair loss inhibition efficacy. The efficacies of nine extracts were compared with minoxidil as control. Efficacy was defined as a rise in the expression levels of IGF-1, VEGF, and Wnt5α but a decrease in DKK-1 and TGF-β2. Intracellular concurrent imaging of these proteins was successfully achieved using HCS, employing visible-to-near infrared probing based on quantum-antibody conjugates and hypermulticolor imaging. © 2012 Society for Laboratory Automation and Screening.
Lee K.-J.,Chungbuk National University |
Park Y.,Korea forest Research Institute |
Kim J.-Y.,Saimdang Cosmetics Co. |
Jeong T.-K.,Saimdang Cosmetics Co. |
And 3 more authors.
Journal of Plant Biotechnology | Year: 2015
This study was conducted to investigate the productivity of biomass and antioxidant compounds in Polygonum multiflorum by culturing explants in air-lift bioreactor containing Murashige and Skoog (MS) medium, by adding different concentrations of auxins [indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA)], sucrose, methyl jasmonate (MeJA), and salicylic acid (SA). Results of this study revealed that the explants culturing on the medium supplemented with 9.84 μM IBA and 50 g/L sucrose were observed to have higher productivity of biomass and bioactive compound than other treatments used. Thus, we expect that these results will be helpful for large-scale production of biomass and antioxidant compounds from Polygonum multiflorum. © Korean Society for Plant Biotechnology.
Lee M.H.,Daejeon Health Science College |
Shon Y.-S.,California State University, Long Beach |
Yoon K.-S.,Saimdang Cosmetics Co.
Bulletin of the Korean Chemical Society | Year: 2015
A rapid, sensitive, and selective method involving ultraperformance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) was developed for the qualitative and quantitative analysis of the constituents in a medicinal herbal complex extract (MHCE). The MHCE was obtained from 23 herbs traditionally used in oriental medicines that we have been developed as new therapeutic materials to prevent hair loss and enhance hair growth. The chromatographic analysis of the samples of MHCE was performed on a reversed-phase C18 (10 cm × 2.1 mm, flow rate 0.3 mL/min) column with gradient elution using acetonitrile and water with 0.1% formic acid. Mass spectrometry was performed in the positive ESI mode. The bioactive compounds were detected by selected reaction monitoring (SRM) with UPLC-ESI-MS/MS. All calibration curves of reference compounds showed good linearity (r2 > 0.9964) in the test ranges. The limit of detection and limit of quantification of the method were 0.02 ng/mL and 0.05 ng/mL, respectively. © 2015 Korean Chemical Society, Seoul & Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Kim M.J.,Saimdang Cosmetics Co. |
Im K.R.,Saimdang Cosmetics Co. |
Yoon K.-S.,Saimdang Cosmetics Co.
Journal of the Korean Society of Food Science and Nutrition | Year: 2015
For the purpose of investigating the in vitro antidiabetic activity of a medicinal herb and herb mixture extracts prepared through traditional antidiabetic prescription, this study examined α-glucosidase inhibitory activity. Tyrosinase, a type I membrane glycoprotein, is synthesized and glycosylated in the endoplasmic reticulum (ER) and Golgi. The enzyme is subsequently transported to melanosomes, where it participates in melanogenesis. Previous studies showed that disruption of early ER N-glycan processing by an α-glucosidase inhibitor suppresses tyrosinase enzymatic activity and melanogenesis. According to the results, most oriental medicinal herbal extracts were stronger than acarbose and N-butyldeoxynojirimycin, known as an α-glucosidase inhibitor. Interestingly, ethyl acetate layer of enzyme hydrolyzed Cheongsimyeonjaeum had an inhibitory effect on melanin synthesis in B16F1 cells, although it did not inhibit tyrosinase activity directly. Together, α-glucosidase inhibition activity could be used to evaluate anti-melanogenesis, although cross-checking with melanin inhibitory assay is recommended. © 2015 Korean Society of Food Science and Nutrition. All rights reserved.
Saimdang Cosmetics Co. | Date: 2010-04-27
[ Fabric softeners for laundry use; ] cosmetic creams; lipsticks; shampoo; badian essential oils for personal use; blushers; eye shadows; cosmetic suntan lotions; foundation creams in the nature of facial creams; perfumes; lavender oil; cosmetic perfumes; beauty mask; cosmetic soaps; dentifrices [ ; shoe polish; polishing wax, namely, floor wax; emery paper, namely, emery boards ].
Fabric softeners for laundry use; cosmetic creams; lipsticks; shampoo; badian essential oils for personal use; blushers; eye shadows; cosmetic suntan lotions; foundation creams in the nature of facial creams; perfumes; lavender oil; cosmetic perfumes; beauty mask; cosmetic soaps; dentifrices; shoe polish; polishing wax, namely, floor wax; emery paper, namely, emery boards.
Saimdang Cosmetics Co. | Date: 2015-07-23
Exfoliant cosmetics, namely, exfoliant creams, non-medicated exfoliating preparations for skin; functional cosmetics, namely, foundation, cosmetic sunscreen preparations; anti-aging cosmetic preparations; cosmetic preparations for the scalp; cosmetic massage creams; make-up; cosmetic preparations for skin pore tightening; make-up for the body; body creams; body skin cleanser; skin toners for the body; non-foaming cosmetic preparations for skin, face and body; sun creams not for medicinal use; cosmetics containing vitamins; biotechnological cosmetics, namely, non-medicated herbal cosmetics; shower cologne being fragranced shower gels; sun block preparations; beauty serums; wrinkle-minimizing cosmetic preparations for topical facial use; cosmetics.
Saimdang Cosmetics Co. | Date: 2015-12-17
Exfoliant cosmetics in the nature of exfoliating creams, Functional cosmetics in the nature of cosmetic sunscreen preparations, Anti-aging cosmetic preparations, Cosmetic preparations for the scalp, Cosmetics for massage in the nature of massage creams, Make-up, Cosmetics in a pack in the nature of pore tightening preparations for use on the skin, Make-up for the body, Body creams, Body cleanser, Toners for the body, Non-foaming cosmetic preparations, Sun creams not for medicinal use, Cosmetics containing vitamin; biotechnological cosmetics, namely, non-medicated herbal cosmetics; Shower cologne in the nature of fragranced shower gels, Sun block cosmetics, Cosmetics in the nature of beauty serums and non-medicated skin serum, Wrinkle-minimizing cosmetic preparations for topical facial use, Cosmetics.
PubMed | Saimdang Cosmetics Co.
Type: | Journal: Journal of ethnopharmacology | Year: 2016
Cornus walteri Wangerin has been used in oriental traditional medicine for the treatment of antidiarrheal and inflammation.The efficacy of Cornus walteri Wangerin on skin anti-photoaging was investigated.Hydrolyzed Cornus walteri Wangerin leaf was tested for the anti-photoaging effects against ultraviolet B (UVB)-induced matrix metalloproteinase (MMP)-1, pro-inflammatory cytokines using human reconstituted skin (KeraSkin-FT) and also tested for elastase activity in vitro. The MMP-1 and pro-inflammatory cytokine levels of the extract were evaluated by enzyme-linked immunosorbent assay (ELISA).The extract of hydrolyzed Cornus walteri Wangerin leaf (CWE) had the elastase inhibitory activity (ICCWE is a promising anti-photoaging agent for the treatment of UVB-induced skin.