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Bhopal, India

Singh R.K.,National Botanical Research Institute | Sane V.A.,National Botanical Research Institute | Misra A.,National Botanical Research Institute | Ali S.A.,Saifia Science College | Nath P.,National Botanical Research Institute

Alcohol dehydrogenases play an important role during fruit ripening and aroma production. Three full-length cDNAs (MiAdh1, 2 and 3) encoding alcohol dehydrogenases were obtained from mango fruit pulp using RT-PCR approaches. All three members displayed strong homology in the coding region when compared at the protein and nucleotide levels, however showed variations in untranslated regions. Expression patterns of these ADHs were different during fruit development and ripening. MiADH1 and MiADH2 transcripts accumulated at the onset of ripening in mango fruit whereas MiADH3 accumulated during early development of fruit. Expression analysis also indicated that mango ADHs were responsive to ethylene but regulated differently by ABA. MiADH1 was induced by ABA treatment whereas MiADH2 transcript was negatively regulated by ABA. MiADH3 did not respond to ABA in ripening fruit. Differences in substrate specificity for NADH and NADPH were also observed between the three enzymes. Total ADH enzyme activity correlated positively with increased transcript levels at the initiation of ripening. © 2010 Elsevier Ltd. Source

Complexes of transition metals like Cu(II) and Ni(II) with Glipizide 1-cyclohexyl 3[[p[2(5methyl pyrazine carboxamido) ethyl] phenyl] Sulphonyl] urea were synthesized. Metal complexes were characterized by elemental analysis, IR and NMR. The crystal structure of complexes was determined by X-ray diffraction method. The XRD data was used to calculate various parameters like crystal system, volume, density, porosity, particle size etc. based on these studies tetrahedral geometry has been proposed for these complexes. Source

Mishra P.,National Bureau of Animal Genetic Resources | Mishra P.,Saifia Science College | Ali A.S.,National Bureau of Animal Genetic Resources | Verma N.K.,National Bureau of Animal Genetic Resources
Indian Journal of Animal Sciences

Bundelkhandi goats are found in Bundelkhand region of Madhya Pradesh and Uttar Pradesh. The morphological features are jet black coat color, long legs, narrow face, roman type nose, long hair on body, black eyelids and muzzle, long curly and pendulous ears and bushy tail. The overall average recorded for body length, height at withers, chest girth, paunch girth, face length, horn length, ear length, tail length and body weights in bundelkhandi goats managed under any system were recorded. The males reared at farm exhibited better growth than those of field but female goats reared under two different management systems do not show significant difference in their growth. Genetic diversity was analysed in randomly selected unrelated 50 goats using FAO recommended 25 microsatellite markers. The observed number of allele ranged from 2 to 20 with an overall mean of 11.08 and the effective average number of allele was 4.96. The PIC value ranged between 0.41 to 0.92 and the highest value of the observed heterozygosity was 0.94 and the lowest was 0.06 and the expected heterozygosity was 0.73 to 0.89 respectively. The bottleneck analysis revealed the absence of any bottleneck in Bundelkhandi goat in recent past. Source

Singh R.K.,CSIR - Central Electrochemical Research Institute | Ali S.A.,Saifia Science College | Nath P.,CSIR - Central Electrochemical Research Institute | Sane V.A.,CSIR - Central Electrochemical Research Institute
Journal of Experimental Botany

Mango is characterized by high tocopherol and carotenoid content during ripening. From a cDNA screen of differentially expressing genes during mango ripening, a full-length p-hydroxyphenylpyruvate dioxygenase (MiHPPD) gene homologue was isolated that encodes a key enzyme in the biosynthesis of tocopherols. The gene encoded a 432-amino-acid protein. Transcript analysis during different stages of ripening revealed that the gene is ripening related and rapidly induced by ethylene. The increase in MiHPPD transcript accumulation was followed by an increase in tocopherol levels during ripening. The ripening-related increase in MiHPPD expression was also seen in response to abscisic acid and to alesser extent to indole-3-acetic acid. The expression of MiHPPD was not restricted to fruits but was also seen in other tissues such as leaves particularly during senescence. The strong ethylene induction of MiHPPD was also seen in young leaves indicating that ethylene induction of MiHPPD is tissue independent. Promoter analysis of MiHPPD gene in tomato discs and leaves of stable transgenic lines of Arabidopsis showed that the cis elements for ripening-related, ethylene-responsive, and senescence-related expression resided within the 1590 nt region upstream of the ATG codon. Functionality of the gene was demonstrated by the ability of the expressed protein in bacteria to convert p-hydroxyphenylpyruvate to homogentisate. These results provide the first evidence for HPPD expression during ripening of a climacteric fruit. © 2011 The Author(s). Source

Prakash O.,Saifia Science College | Krishan B.,Saifia Science College | Jacob G.,Blyth Academy
Oriental Journal of Chemistry

Glipizide is a second generation sulphonylurea with promising hypoglycemic activity. It acts by stimulating the release of insulin from β-cells of pancreas. The present paper describes the synthesis and characterization of cerium complex with Glipizide. The conductometric titration using monovariation method reveal that complex is non-ionic and ML2 type which was further confirmed by Job's method of continuous variation as modified by Turner and Anderson. The analytical data agree with the molecular formula (C 21H26N5O4S)2Ce(OH 2)2, that the structure of complex was assigned as octahedral in which ligand molecules lies horizontally joining the central cerium atom. IR, NMR confirms the co-ordination of sulphonyl oxygen on one side and enolic oxygen attached from other side with the metal ion. The structure of complex was proposed on the basis of analytical data, elemental analysis, IR, 1H NMR and X-ray Diffraction studies. Source

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