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Rafey A.,Saifia Science College | Iqbal S.A.,Saifia Science College | Khan Z.,King Abdulaziz University
Journal of Colloid and Interface Science | Year: 2011

Spectrophotometric, kinetic, and transmission electron microscopic (TEM) data for the formation of Ag-nanoparticles using aspartic acid (Asp) as reductant are reported for the first time. In the formation of transparent silver sols, an alkaline medium is required. The silver nanoparticles are spherical, uniform particle size, and strongly depend on the [Asp]. The apparent rate constant decreases with [Asp] (from 4.0 to 24.0×10 -4moldm -3, the rate constants decreased from 2.6×10 -4 to 0.3×10 -4s -1). For a certain reaction time, i.e., 30min, the absorbance of the silver sol first increased until it reached a maximum, and then decreased with [Asp]. Kinetic and TEM results indicate that the size of the Ag-nanoparticles depends on the [Asp]. It is proposed that the oxidation of Asp occurs by the adsorbed Ag + ions on the surface of Ag 2O particles. © 2010 Elsevier Inc.


Singh R.K.,National Botanical Research Institute | Sane V.A.,National Botanical Research Institute | Misra A.,National Botanical Research Institute | Ali S.A.,Saifia Science College | Nath P.,National Botanical Research Institute
Phytochemistry | Year: 2010

Alcohol dehydrogenases play an important role during fruit ripening and aroma production. Three full-length cDNAs (MiAdh1, 2 and 3) encoding alcohol dehydrogenases were obtained from mango fruit pulp using RT-PCR approaches. All three members displayed strong homology in the coding region when compared at the protein and nucleotide levels, however showed variations in untranslated regions. Expression patterns of these ADHs were different during fruit development and ripening. MiADH1 and MiADH2 transcripts accumulated at the onset of ripening in mango fruit whereas MiADH3 accumulated during early development of fruit. Expression analysis also indicated that mango ADHs were responsive to ethylene but regulated differently by ABA. MiADH1 was induced by ABA treatment whereas MiADH2 transcript was negatively regulated by ABA. MiADH3 did not respond to ABA in ripening fruit. Differences in substrate specificity for NADH and NADPH were also observed between the three enzymes. Total ADH enzyme activity correlated positively with increased transcript levels at the initiation of ripening. © 2010 Elsevier Ltd.


Prakash O.,Saifia Science College | Krishan B.,Saifia Science College | Jacob G.,Blyth Academy
Oriental Journal of Chemistry | Year: 2013

Glipizide is a second generation sulphonylurea with promising hypoglycemic activity. It acts by stimulating the release of insulin from β-cells of pancreas. The present paper describes the synthesis and characterization of cerium complex with Glipizide. The conductometric titration using monovariation method reveal that complex is non-ionic and ML2 type which was further confirmed by Job's method of continuous variation as modified by Turner and Anderson. The analytical data agree with the molecular formula (C 21H26N5O4S)2Ce(OH 2)2, that the structure of complex was assigned as octahedral in which ligand molecules lies horizontally joining the central cerium atom. IR, NMR confirms the co-ordination of sulphonyl oxygen on one side and enolic oxygen attached from other side with the metal ion. The structure of complex was proposed on the basis of analytical data, elemental analysis, IR, 1H NMR and X-ray Diffraction studies.


Singh R.K.,CSIR - Central Electrochemical Research Institute | Ali S.A.,Saifia Science College | Nath P.,CSIR - Central Electrochemical Research Institute | Sane V.A.,CSIR - Central Electrochemical Research Institute
Journal of Experimental Botany | Year: 2011

Mango is characterized by high tocopherol and carotenoid content during ripening. From a cDNA screen of differentially expressing genes during mango ripening, a full-length p-hydroxyphenylpyruvate dioxygenase (MiHPPD) gene homologue was isolated that encodes a key enzyme in the biosynthesis of tocopherols. The gene encoded a 432-amino-acid protein. Transcript analysis during different stages of ripening revealed that the gene is ripening related and rapidly induced by ethylene. The increase in MiHPPD transcript accumulation was followed by an increase in tocopherol levels during ripening. The ripening-related increase in MiHPPD expression was also seen in response to abscisic acid and to alesser extent to indole-3-acetic acid. The expression of MiHPPD was not restricted to fruits but was also seen in other tissues such as leaves particularly during senescence. The strong ethylene induction of MiHPPD was also seen in young leaves indicating that ethylene induction of MiHPPD is tissue independent. Promoter analysis of MiHPPD gene in tomato discs and leaves of stable transgenic lines of Arabidopsis showed that the cis elements for ripening-related, ethylene-responsive, and senescence-related expression resided within the 1590 nt region upstream of the ATG codon. Functionality of the gene was demonstrated by the ability of the expressed protein in bacteria to convert p-hydroxyphenylpyruvate to homogentisate. These results provide the first evidence for HPPD expression during ripening of a climacteric fruit. © 2011 The Author(s).


Iqbal S.A.,Saifia Science College | Jose S.,Research Laboratory | Jacob G.,Saifia Science College
Oriental Journal of Chemistry | Year: 2011

Metal complexes of manganese (II) and cobalt (II) have been synthesized with active hypoglycemic agent (glimepiride), an oral antidiabetic drug. The elemental analyses of the ligand as well as metal complexes indicate that complexes having 2:1 stoichiometry of the type (C 24H 34N 4O 5S) 2 M.2H 2O, where M=Mn(II) and Co(II). The complexes have been characterized by IR spectra, electronic spectral and molar conductance data. Infrared spectral studies confirm the co-ordination of sulphonyl oxygen on one side and enolic oxygen attached from other side with the metal ion. On the basis of electronic spectral values the complexes are proposed to have octahedral geometry. The molar conductivity also reveals that the complexes are non-ionic in nature.


Mishra P.,National Bureau of Animal Genetic Resources | Mishra P.,Saifia Science College | Ali A.S.,National Bureau of Animal Genetic Resources | Verma N.K.,National Bureau of Animal Genetic Resources
Indian Journal of Animal Sciences | Year: 2012

Bundelkhandi goats are found in Bundelkhand region of Madhya Pradesh and Uttar Pradesh. The morphological features are jet black coat color, long legs, narrow face, roman type nose, long hair on body, black eyelids and muzzle, long curly and pendulous ears and bushy tail. The overall average recorded for body length, height at withers, chest girth, paunch girth, face length, horn length, ear length, tail length and body weights in bundelkhandi goats managed under any system were recorded. The males reared at farm exhibited better growth than those of field but female goats reared under two different management systems do not show significant difference in their growth. Genetic diversity was analysed in randomly selected unrelated 50 goats using FAO recommended 25 microsatellite markers. The observed number of allele ranged from 2 to 20 with an overall mean of 11.08 and the effective average number of allele was 4.96. The PIC value ranged between 0.41 to 0.92 and the highest value of the observed heterozygosity was 0.94 and the lowest was 0.06 and the expected heterozygosity was 0.73 to 0.89 respectively. The bottleneck analysis revealed the absence of any bottleneck in Bundelkhandi goat in recent past.


Complexes of transition metals like Cu(II) and Ni(II) with Glipizide 1-cyclohexyl 3[[p[2(5methyl pyrazine carboxamido) ethyl] phenyl] Sulphonyl] urea were synthesized. Metal complexes were characterized by elemental analysis, IR and NMR. The crystal structure of complexes was determined by X-ray diffraction method. The XRD data was used to calculate various parameters like crystal system, volume, density, porosity, particle size etc. based on these studies tetrahedral geometry has been proposed for these complexes.


Ali S.A.,Saifia Science College | Naaz I.,Saifia Science College | Choudhary R.K.,Saifia Science College
Journal of Receptors and Signal Transduction | Year: 2014

Reduced production of melanin by decreased or the absence of melanocytes leads to various hypopigmentation disorders, and the development of melanogenetic agents for photoprotection and hypopigmentation disorders is one of the top priority areas of research. Hence, the present study was carried out to elucidate the ability of berberine, a principal active ingredient present in the roots of the herb Berberis vulgaris to stimulate pigment dispersion in the isolated skin melanophores of the toad Bufo melanostictus. In the present study, mean melanophore size index of the isolated skin melanophores of B. melanostictus was assayed after treating with various concentrations of berberine. A marked melanin dispersion response leading to skin darkening was observed in the isolated melanophores of toad in response to berberine, which was found to be mediated through beta-2 adrenergic receptors. The physiologically significant dose-related melanin dispersion effects of berberine per se were found to be completely abolished by propranolol, which is a specific beta-2 adrenergic receptor blocker. These per se melanin dispersal effects were also found to be markedly potentiated by isoprenaline, which is a specific beta-adrenoceptor agonist. The results indicate that berberine causes a tremendous, dose-dependent, physiologically significant pigment dispersing in the isolated skin melanophores of B. melanostictus. © 2014 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted.


Prasad S.,Saifia Science College | Ali S.A.,Saifia Science College | Vijh R.K.,National Bureau of Animal Genetic Resources
Journal of Camel Practice and Research | Year: 2015

The study included genetic and demographic bottleneck analysis of Malvi camel breeds by microsatellite markers using 29 loci. The genetic diversity of a population of Malvi camel was evaluated. Multilocus genotype data on 29 microsatellites were generated on 138 Malvi camels. A total of 240 alleles were observed. The mean observed heterozygosity in camel population for all the 29 loci was found to be 0.6041 ± 0.3256 while the mean expected heterozygosity was 0.5978 ± 0.1983. The statistical values for within locus k-test obtained for the Malvi camel was 18 loci with negative values which signifies population expansion. The inter locus g-test revealed a value of 2.3557. This test also revealed that Malvi camel population has undergone population expansion. Three tests viz; Sign test (Non-parametric), Standardised Differences test (Parametric), Wilcoxon test and Qualitative graphical method based on the allele frequency spectra reflects that the population had not undergone bottleneck.


PubMed | Saifia Science College
Type: | Journal: Mini reviews in medicinal chemistry | Year: 2016

Skin pigmentation is a broadly appearing phenomenon in nature which plays an important task of determining the appearance and biology of all vertebrates including human beings. Skin color is a crucial attribute, determined by the synthesis of melanin pigment within melanocytes by the process of melanogenesis and is regulated by many extrinsic as well as intrinsic factors. Tyrosinase catalyzes the key step of melanogenesis, dysfunction of tyrosinase leads to reduce melanin production which results in severe clinical and aesthetical problems of hypopigmentation. Therefore, the regulation of melanin production is an important strategy in the treatment of abnormal skin pigmentation for cosmetic and medicinal purpose. The present review covers the various aspects of mammalian melanocyte biology which will help in the identification of key regulators of melanogenesis from pharmaceutical and pharmacological point of view. Further sections of the review focus on the dysfunctions of melanogenic pathways, which result in severe clinical and aesthetical problems of hypopigmentation. We have also attempted to highlight the ability of available scientifically validated plant extracts to naturally enhance melanin synthesis in order to cure hypopigmentation.

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