Sai Institute of Paramedical and Allied science

Dehradun, India

Sai Institute of Paramedical and Allied science

Dehradun, India
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Sharma N.,Shri Guru Ram Rai Institute of Medical and Health science | Nautiyal S.C.,Shri Guru Ram Rai Institute of Medical and Health science | Ahmed M.Z.,Shri Guru Ram Rai Institute of Medical and Health science | Saini N.,P.A. College | And 5 more authors.
International Journal of Toxicological and Pharmacological Research | Year: 2013

Herpes simplex virus (HSV) encephalitis is a serious infection but diagnosis previously required brain biopsy in certain cases due to the low sensitivity of cerebrospinal fluid (CSF) culture and serology. PCR allows the detection of HSV DNA from CSF with 95% sensitivity thus avoiding invasive brain biopsy. HSV type 1and type 2 is highly contagious, and can infect skin, mouth and genital organs, both in male and females. Present study, includes the genotyping of HSV type 1 and 2 with the utilization of DPO (dual priming oligonucleotides) primers by conventional PCR. DPO PCR detected 2 out of 8 specimens with HSV infection. Though, study needs the trials of the technology on more number of specimens which can be useful as compared to serological or culture techniques for Herpes Simplex Virus.


Agrawal P.K.,G B Pant Engineering College | Agrawal S.,Sai Institute of Paramedical and Allied science | Shrivastava R.,Jaypee University of Information Technology
3 Biotech | Year: 2015

Biosphere is a store house of various microorganisms that may be employed to isolate and exploit microbes for environmental, pharmaceutical, agricultural and industrial applications. There is restricted data regarding the structure and dynamics of microbial communities in several ecosystems because only a little fraction of microbial diversity is accessible by culture methods. Owing to limitations of traditional enrichment methods and pure culture techniques, microbiological studies have offered a narrow portal for investigating microbial flora. The bacterial community represented by the morphological and nutritional criteria failed to provide a natural taxonomic order according to the evolutionary relationship. Genetic diversity among the isolates recovered from mushroom compost has not been widely studied. To understand genetic diversity and community composition of the mushroom compost microflora, different approaches are now followed by taxonomists, to characterize and identify isolates up to species level. Molecular microbial ecology is an emerging discipline of biology under molecular approach which can provide complex community profiles along with useful phylogenetic information. The genomic era has resulted in the development of new molecular tools and techniques for study of culturable microbial diversity including the DNA base ratio (mole% G + C), DNA–DNA hybridization, DNA microarray and reverse sample genome probing. In addition, non-culturable diversity of mushroom compost ecosystem can be characterized by employing various molecular tools which would be discussed in the present review. © 2015, The Author(s).


Abhishek M.,Sai Institute of Paramedical and Allied science | Vinay G.,Sai Institute of Paramedical and Allied science | Verma Satish K.,Sai Institute of Paramedical and Allied science | Singh Santosh K.,Gayatri Institute of Biomedical Science | And 3 more authors.
International Journal of Current Pharmaceutical Review and Research | Year: 2011

Leucas cephalotes (Labiatae) is an annual herb found in Uttarakhand and throughout India as a weed in cultivated fields, wastelands and roadsides. In the present investigation the phytochemical screening of the crude methanolic extract of leaves was done by conventional methods and anti-inflammatory activity of crude, alkaloid, aqueous, hexane, petroleum ether and non alkaloid fractions of the leaves were investigated using carrageenan induced rat paw edema method. The results showed that alkaloidal fractions of the leaves causes significant reduction in inflammation i.e 80 % (100 mg/kg) followed by crude methanol extract i.e 61 % (100 mg/kg) and aqueous extract i.e 58 % (100 mg/kg) as compared to standard anti-inflammatory drug aspirin i.e 68.62% (25mg/kg). However, non alkaloidal, hexane and petroleum ether fractions did not show any anti-inflammatory activity irrespective of the time intervals. Thus crude methanolic extract and alkaloidal fractions of leaves of the plant can be fully explored for its anti-inflammatory potential.


Mathur A.,Sai Institute of Paramedical and Allied science | Sharma V.,Sai Institute of Paramedical and Allied science | Bhardwaj A.,Sai Institute of Paramedical and Allied science | Yousuf S.,Sai Institute of Paramedical and Allied science | And 3 more authors.
Journal of Chemical and Pharmaceutical Research | Year: 2011

In the present investigation, pectin, the polysaccharide content in fruits is used as a basis for screening different varieties of Apple (Pyrus malus L.) of the same season of Kashmir (J&K). Different varieties of apple fruit of Kashmir (J&K) of the same season viz American, Delicious and Maharaj-ji were collected from the local gardens of Kashmir and pectin content present was extracted. The yield of pectin content was found to be maximum in Maharaj-ji (20.60%) followed by Delicious (14.40%) and American (11.60%). The pectin extracted was then evaluated for its in vitro antibacterial activity against different pathogenic bacterial cultures. The results investigated that pectin extracted from Delicious variety showed potent antibacterial activity against Klebsiella pneumoniae (MIC value: 0.8 mg/ml) followed by Streptococcus pyogenes (MIC value: 0.3 mg/ml), E.coli (MIC value: 0.7 mg/ml) and Lactococcus sp. (MIC value: 0.7 mg/ml). The pectin extracted from other varieties showed no potency against any of the bacterial cultures. Further the pectin extracted from each of the variety was evaluated for in vitro antifungal activity against Aspergillus niger, Candida albicans and Saccharomyces cerevisae. It was observed that pectin extracted from any of the variety showed no potency against any of the test fungal cultures. The results of the antimicrobial activity of the pectin extracted from each of the varieties were compared with that of standard pectin and Azithromycin. It was observed that standard pectin also not showed antifungal activity similarly to that of extracted pectin. The results thus confirmed that pectin can be utilized as a potent antibacterial agent and can be utilized as an index for screening different apple varieties of Kashmir (J & K). Furthermore our study validates the use of Delicious variety for the treatment of the infections borne by the tested organisms. The pectin extracted was further assayed for cellular toxicity against sheep fresh blood erythrocytes but no hemolysis against sheep fresh blood erythrocytes was observed.


Shruti A.,Sai institute of paramedical and allied science | Kumar V.S.,Sai institute of paramedical and allied science | Kumar S.S.,SBS PG Institute of Biomedical Science and Research | Prasad S.K.,Motilal Nehru National Institute of Technology
Asian Journal of Pharmaceutical and Clinical Research | Year: 2011

Natural ecosystems are directly dependent on beneficial microorganisms present in the rhizosphere for soil health and plant productivity. Plant growth promoting bacteria were isolated from soil of central and upper Himalayan region with a view to screen/evaluate their phylogenetic relationship among the isolates by using amplified Restriction DNA Analysis (ARDRA).


Mathur A.,National Institute of Malaria Research | Verma S.K.,Sai Institute of Paramedical and Allied science | Yousuf S.,Sai Institute of Paramedical and Allied science | Singh S.K.,Sai Institute of Paramedical and Allied science | And 2 more authors.
International Journal of Pharma and Bio Sciences | Year: 2011

Antimicrobial activity of various extracts of roots (200mg/ml) of Ricinus communis were screened against pathogenic microorganisms such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella typhimurium, Proteus vulgaris, Bacillus subtilis, Candida albicans and Aspergillus niger using well diffusion method. Aqueous extracts don't show any significant antimicrobial properties. The hexane and methanol extracts revealed maximum antimicrobial activity (p<0.0001). These findings established the potential of the roots of Ricinus communis as an effective antimicrobial agent. However, further studies are needed to evaluate active compounds and probable medicinal benefits in chemotherapy among humans.


Mathur A.,Sai Institute of Paramedical and Allied science | Bhat R.,Sai Institute of Paramedical and Allied science | Prasad G.B.K.S.,Jiwaji University | Dua V.K.,Bharat Heavy Electricals Ltd. | And 2 more authors.
Rasayan Journal of Chemistry | Year: 2010

The antimicrobial activity and minimum inhibitory concentration (MIC) of various plant extracts in different solvents such as ethanol (98%), hexane (99%) and distilled water of plants traditionally used as medicines as Bidens pilosa L., Bixa orellana L., Cecropia peltata L., Cinchona officinalis L., Gliricidia sepium, Jacaranda mimosifolia, Justica secunda Vahl., Piper pulchrum, P. paniculata L. and Spilanthes americana were evaluated against five bacteria Staphylococcus aureus, Streptococcus β hemolytic, Bacillus cereus, Pseudomonas aeruginosa, Escherichia coli and fungus Candidia albicans. These plants are used in Indian folk medicine to treat infections of microbial origin. © 2010 RASĀYAN. All rights reserved.

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