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Nimbalkar M.S.,Shivaji University | Nimbalkar M.S.,Sahyadri Group for Enriching Nature | Pai S.R.,Regional Medical Research Center | Pai S.R.,Sahyadri Group for Enriching Nature | And 4 more authors.
Food Chemistry | Year: 2012

Reliable and reproducible techniques for identification and quantification of amino acids usually require derivatization. However, techniques such as LC-MS/MS may perhaps sideline the derivatization with significant accuracy. Understanding the interaction between free amino acids and several physiological processes apparently requires that the pool be studied in the seed itself. Data on free amino acid content of grain amaranth is very inadequate. The present analysis revealed a higher concentration of essential amino acids especially Threonine and Tryptophan than non essential amino acids. The study constitutes first report of use of LC-MS/MS method for analysing the free amino acid composition of amaranth grain. © 2012 Elsevier Ltd. All rights reserved.


Pai S.R.,Shivaji University | Nimbalkar M.S.,Shivaji University | Pawar N.V.,Shivaji University | Patil R.P.,Sahyadri Group for Enriching Nature | Dixit G.B.,Shivaji University
International Journal of Pharma and Bio Sciences | Year: 2010

Seasonal discrepancy in the antioxidant potential from bark of Nothapodytes nimmoniana was evaluated using two different methods (DPPH and FRAP) and were related to total phenolic content as well as flavonoid content. Higher yield of phenolic and flavonoid were observed during summer (May) similarly, the antioxidant activities (DPPH & FRAP) also were high. The antioxidant activities were strongly correlated with total phenolics (DPPH, FRAP) in all seasons, the maximum being during the summer (May) and minimum during monsoon (August). Total phenolic content showed highest correlation with DPPH and FRAP antioxidant assays during the month of winter (December). A specific pattern of increase from August-December-May was observed in phenolic content and antioxidant activities (FRAP and DPPH) tested for respective standards.

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