Sagar Institute of Research and Technology Pharmacy

Bhopal, India

Sagar Institute of Research and Technology Pharmacy

Bhopal, India

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Jain N.,Sagar Institute of Research and Technology Pharmacy | Jain R.,Sagar Institute of Research and Technology Pharmacy | Kulkarni S.,Sagar Institute of Research and Technology Pharmacy | Jain D.K.,Truba Institute of Pharmacy | Jain S.,Sagar Institute of Research and Technology Pharmacy
Journal of Chemical and Pharmaceutical Research | Year: 2011

A novel, safe, accurate, sensitive and economic Spectrophotometric method was developed by application of mixed hydrotropy using 2 M sodium acetate and 8 M urea solution (50:50% V/V) as hydrotropic solubilizing agent for the quantitative determination of poorly water-soluble pramipexole dihydrochloride (solubility:- 1.4 × 10-01 mg/ml in water) in tablet dosage form. The solubility of Pramipexole Dihydrochloride increases more than 46 times in mixed hydrotropic solution as compared to solubility in distilled water. Pramipexole dihydrochloride shows maximum absorbance at 262 nm. Sodium acetate, urea and other tablets excipents did not show any absorbance above 250 nm and thus no interference in the estimation was seen. Pramipexole dihydrochloride was obeyed Lambert Beer's law in the concentration range of 15 to 75 μg/ml (r2= 0.9997) in mixed hydrotropic agent with mean recovery was found 98.35±0.55%. The percent concentration in marketed tablet formulation was found 97.52±1.65%. Parameters such as linearity, precision, accuracy, specificity and robustness were studied as reported in the International Conference on Harmonization guidelines. The relative standard deviations for three replicate measurements in five concentrations of samples were always less than 2%. So this method can successfully employ in the routine analysis of pramipexole dihydrochloride in bulk drug and tablet dosage forms.


Jain N.,Sagar Institute of Research and Technology Pharmacy | Jain R.,Sagar Institute of Research and Technology Pharmacy | Jain D.K.,Sagar Institute of Research and Technology Pharmacy | Maheshwari R.K.,A And G Pharmaceutical, Inc. | Jain S.,Sagar Institute of Research and Technology Pharmacy
Pakistan Journal of Pharmaceutical Sciences | Year: 2013

A novel, eco friendly, accurate, sensitive, economic and safe spectrophotometric method was developed by application of mixed hydrotropy using 2 M sodium acetate, 8 M urea, 2 M niacinamide and 2 M sodium benzoate solution (25:25:25:25% V/V) as hydrotropic agent, for the solubalizing of poorly water-soluble Furazolidone (FZ) (solubility:- 3.64e-01 mg/mL in water). There were more than 32 times enhancements in the solubility of FZ were found in mixed hydrotropic solution as compared to solubilities in distilled water. FZ shows maximum absorbance at 360 nm where sodium acetate, urea, niacinamide, sodium benzoate and other tablets excipients did not show any absorbance above 300 nm, and thus no interference in the estimation was seen. FZ was obeyed Beer's law in the concentration range of 10 to 50 μg/ml (r2=0.9992) in mixed hydrotropic solvent with mean recovery ranging from 97.32% to 98.9%. Proposed method is new, simple, economic, safe, rapid, accurate and reproducible and was validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values.


Sharma M.,Rajiv Gandhi Technical University | Nautiyal P.,Rajiv Gandhi Technical University | Jain S.,Sagar Institute of Research and Technology Pharmacy | Jain D.,Rajiv Gandhi Technical University
Journal of AOAC International | Year: 2010

Combination therapy with acyclovir and zidovudine is used for the treatment of herpes-infected immunocompromised patients. In the view of the optimal drug concentrations (minimum effective concentrations) for viral suppression and avoidance of drug toxicity, monitoring of drug levels has been considered essential to determine drug concentrations in plasma after administration of a dose of acyclovir and zidovudine. A simple, precise, and rapid RP-HPLC method has been developed for this purpose. Chromatographic separation was performed using methanol-water (50 + 50, v/v), pH 2.5 adjusted with orthophosphoric acid, as an isocratic mobile phase at a flow rate of 0.8 mL/min with an Inertsil ODS (C18) column (5 |μm particle size, 250 × 4.60 mm id). Detection was carried out using a UV photo diode array detector at 258 nm. The plasma samples were prepared by a protein precipitation method. The retention time for acyclovir and zidovudine was 3.5 ± 0.2 and 6.2 ± 0.3 min, respectively. The method was linear in the range of 200-1800 and 400-3600 ng/mL with LOQ of 200 ng (SD = ±1.4) and 400 ng (SD = ±0.9) for zidovudine and acyclovir, respectively, in plasma. The mean accuracy was 98.0 and 96.4%, with average extraction recovery of 64.8 ± 2.1 and77.5 ± 1.7% for lower nominal concentrations of acyclovir and zidovudine, respectively.


Jain N.,Sagar Institute of Research and Technology Pharmacy | Jain R.,Sagar Institute of Research and Technology Pharmacy | Thakur N.,Sagar Institute of Research and Technology Pharmacy | Gupta B.P.,Sagar Institute of Research and Technology Pharmacy | And 3 more authors.
Asian Journal of Pharmaceutical and Clinical Research | Year: 2010

Nanotechnology is relatively new, and although the full scope of contributions of these technological advances in the field of human health care remains unexplored, recent advances suggest that nanotechnology will have a profound impact on disease prevention, diagnosis, and treatment. Nanotechnology based delivery system would allow faster drug absorption, controlled dosage release into the human body and would have other unique properties of minimizing side-effects by eliminating requirement of co-solvent as used in conventional dosage form. Further, drugs that have side-effects due to triggering an immune system response can be wrapped in nanoparticle coating and prevent immune system from recognizing and reacting to a foreign substance. It is an ideal targeting system should have long circulating time, it should be present at appropriate concentrations at the target site, and it should not lose its activity or therapeutic efficacy while in circulation.


Jain R.,Suresh Gyan Vihar University | Jain V.,Sagar Institute of Research and Technology Pharmacy | Jain N.,Sagar Institute of Research and Technology Pharmacy | Jain D.K.,Truba Institute of Pharmacy | Jain S.,Sagar Institute of Research and Technology Pharmacy
Journal of Applied Pharmaceutical Science | Year: 2012

The present work describes a novel, accurate, sensitive and economic safe spectrophotometric method was developed by application of hydrotropy, using 8 M Urea solution as hydrotropic solubilizing agent, for the quantitative determination of poorly watersoluble lomefloxacin HCl in tablet dosage form. There were more than 43 times enhancements in the solubility of lomefloxacin HCl increases in hydrotropic solution as compared to solubilities in distilled water. Lomefloxacin HCl shows maximum absorbance at 281 nm. Urea and other tablets excipents did not show any absorbance above 230 nm, and thus no interference in the estimation was seen. Lomefloxacin HCl was obeyed Beer,s law in the concentration range of 5 to 25μg/ml (r2= 0.9998) in hydrotropic solvent with mean recovery ranging from 98.03±0.65 to 98.59±0.32%. Proposed method is new, simple, economic, safe, rapid, accurate and reproducible. The developed methods were validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. The method can be used for routine analysis in both research laboratories, and pharmaceutical and chemical industries to analyze the drugs without the use of organic solvents thus make the environment eco-friendly.


Jain R.,Suresh Gyan Vihar University | Jain N.,Sagar Institute of Research and Technology Pharmacy | Jain D.K.,Guru Ghasidas University | Patel V.K.,Guru Ghasidas University | And 2 more authors.
Arabian Journal of Chemistry | Year: 2013

Two simple, accurate, novel, safe and precise methods were developed for the simultaneous estimation of poorly water-soluble drugs Metronidazole (MTR) and Furazolidone (FZ) in a tablet dosage form using 2 M sodium acetate and 8 M urea solution (50:50%V/V) as a mixed hydrotropic solution. MTR and FZ show maximum absorbances at 319 and 364 nm, respectively. Sodium acetate and urea solution did not show any absorbance above 240 nm and thus no interference in the estimation of drugs was seen. MTR and FZ follow Beer's law in the concentration range of 10-50 μg/ml and 5-25 μg/ml (r2 = 0.9992 and 0.9996). Method-A employs the simultaneous equation method using 319 and 364 nm as two analytical wavelengths, method-B employs the absorption ratio method, which uses 339.2 and 364 nm as two analytical wavelengths for estimation of MTR and FZ. The mean percent label claims of tablet dosage were found to be 98.715 ± 1.012 and 98.74 ± 0.912 in method A, 98.99 ± 0.872 and 97.89 ± 0.903 in method B for MTR and FZ, respectively. The developed methods were validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values therefore both methods can be used for routine monitoring of MTR and FZ in industry in the assay of bulk drug and tablets. © 2013.


Jain R.,Suresh Gyan Vihar University | Jain N.,Sagar Institute of Research and Technology Pharmacy | Jain S.K.,Sagar Institute of Research and Technology Pharmacy
Asian Journal of Pharmaceutical and Clinical Research | Year: 2013

Objective: Analysis of drug utilized the organic solvent which are costlier, toxic, and causing environment pollution. Hydrotropic solution may be a proper choice to preclude the use of organic solvents so that an attempt has been made to develop simple, accurate, novel, safe and precise spectrophotometric method for estimation of poorly-water soluble drug meloxicam. Methods: Solubility of meloxicam is increased by using 8% phenol and 25% sodium benzoate solution as hydrotropic agent. There was more than 32 fold solubility enhanced in hydrotropic solution as compare with distilled water. The meloxicam shows the maximum absorbance at 362 nm. At this wavelength hydrotropic agent and other tablet excipients do not shows any significant interference in the spectrophotometric assay. Results: The developed method was found to be linear in the range of 15-75 μg/ml with correlation coefficient (r2) of 0.9994. The mean percent label claims of tablets of meloxicam in two marketed, formulation-I and formulation-II estimated by the proposed method were found to be 98.35±0.76 to 98.53±0.94 respectively. The developed methods were validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. Conclusion: As hydrotropic agent used in the proposed method so this method is eco-friendly and it can be used in routine quantitative analysis of drug in bulk drug and dosage form in industries.


Jain N.,Mahatma Jyoti Rao Phoole University | Singhal S.,Mahatma Jyoti Rao Phoole University | Kumar Jain S.,Sagar Institute of Research and Technology Pharmacy
Asian Journal of Pharmaceutical and Clinical Research | Year: 2013

Objective: Stilbene based compounds are widely represented in nature and have become particular interest because of their wide range of biological activities. The aim of research work was to synthesize many synthetic compounds using stilbene as the essential pharmacophore. Methods: A series of novel P-(substituted phenyl) - 2 (substituted phenyl) Ethene derivatives (1a-1q) have been synthesized by the witting reactions i.e. Substituted benzyl chloride reacted with the phosphonium chloride salt give the P substituted benzyl (chloro) triphenyl phosporane (1) which on further reaction with substituted benzaldehyde yielded the corresponding 1a-1q. Results: The yield of synthesized compound was found to be in the range of 60-85%.Synthesized compounds were characterized by their physiochemical properties like solubility, melting point, IR spectroscopy, 1H NMR spectroscopy, Fab MASS and elemental analysis and result of analysis confirm the structure of synthesize compound. Conclusion: This procedure appears to be a promising and conceptually straightforward route for the synthesis of various Cis-stilbenes. The synthesis and characterization of other phenyl substituted compounds using this method are under investigation, and will be reported in due course.


Jain N.,Sagar Institute of Research and Technology Pharmacy | Jain R.,Sagar Institute of Research and Technology Pharmacy | Jain D.K.,Truba Institute of Pharmacy | Chandel H.S.,Truba Institute of Pharmacy
Natural Product Research | Year: 2010

The aim of the present study was to assess the wound-healing activity of ethanolic extracts of Acorus calamus leaves. A wound was induced by an excision- and incision-based wound model in rats of either sex. The mature green leaves of A. calamus were collected and authenticated. Extractions of dried leaves were carried out with 80% ethanol in a soxhlet apparatus. For wound-healing activity, the extracts were applied topically once daily in conc. of 40% w/w and 20% w/w in the form of ointment and compared with a standard drug (povidion-iodine). The healing of the wound was assessed by the rate of wound closure, period of epithelialisation, tensile strength and weight of the granulation tissue, hydroxyproline content and histopathology of the granulation tissue. The ethanolic extract of A. calamus promoted wound-healing activity significantly in both the wound models studied. The histological study of the granulation tissue with 20% A. calamus extract ointment-treated animals showed a larger number of inflammatory cells and lesser collagen when compared with the 40% A. calamus extract ointment-treated animals. However, this was better than the control group of animals. Enhanced wound contraction, decreased epithelialisation time, increased hydroxyproline content and histological characteristics suggest that A. calamus extract may have therapeutic benefits in wound healing. © 2010 Taylor & Francis.


PubMed | Sagar Institute of Research and Technology Pharmacy
Type: Journal Article | Journal: Pakistan journal of pharmaceutical sciences | Year: 2012

A novel, eco friendly, accurate, sensitive, economic and safe spectrophotometric method was developed by application of mixed hydrotropy using 2 M sodium acetate, 8 M urea, 2 M niacinamide and 2 M sodium benzoate solution (25:25:25:25% V/V) as hydrotropic agent, for the solubalizing of poorly water-soluble Furazolidone (FZ) (solubility:- 3.64e-01 mg/mL in water). There were more than 32 times enhancements in the solubility of FZ were found in mixed hydrotropic solution as compared to solubilities in distilled water. FZ shows maximum absorbance at 360 nm where sodium acetate, urea, niacinamide, sodium benzoate and other tablets excipients did not show any absorbance above 300 nm, and thus no interference in the estimation was seen. FZ was obeyed Beers law in the concentration range of 10 to 50 g/ml (r(2)=0.9992) in mixed hydrotropic solvent with mean recovery ranging from 97.32% to 98.9%. Proposed method is new, simple, economic, safe, rapid, accurate and reproducible and was validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values.

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