Sagar Institute of Research and Technology Pharmacy

Bhopal, India

Sagar Institute of Research and Technology Pharmacy

Bhopal, India
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Jain N.,Sagar Institute of Research and Technology Pharmacy | Jain R.,Sagar Institute of Research and Technology Pharmacy | Kulkarni S.,Sagar Institute of Research and Technology Pharmacy | Jain D.K.,Truba Institute of Pharmacy | Jain S.,Sagar Institute of Research and Technology Pharmacy
Journal of Chemical and Pharmaceutical Research | Year: 2011

A novel, safe, accurate, sensitive and economic Spectrophotometric method was developed by application of mixed hydrotropy using 2 M sodium acetate and 8 M urea solution (50:50% V/V) as hydrotropic solubilizing agent for the quantitative determination of poorly water-soluble pramipexole dihydrochloride (solubility:- 1.4 × 10-01 mg/ml in water) in tablet dosage form. The solubility of Pramipexole Dihydrochloride increases more than 46 times in mixed hydrotropic solution as compared to solubility in distilled water. Pramipexole dihydrochloride shows maximum absorbance at 262 nm. Sodium acetate, urea and other tablets excipents did not show any absorbance above 250 nm and thus no interference in the estimation was seen. Pramipexole dihydrochloride was obeyed Lambert Beer's law in the concentration range of 15 to 75 μg/ml (r2= 0.9997) in mixed hydrotropic agent with mean recovery was found 98.35±0.55%. The percent concentration in marketed tablet formulation was found 97.52±1.65%. Parameters such as linearity, precision, accuracy, specificity and robustness were studied as reported in the International Conference on Harmonization guidelines. The relative standard deviations for three replicate measurements in five concentrations of samples were always less than 2%. So this method can successfully employ in the routine analysis of pramipexole dihydrochloride in bulk drug and tablet dosage forms.


Jain N.,Sagar Institute of Research and Technology Pharmacy | Jain R.,Sagar Institute of Research and Technology Pharmacy | Jain D.K.,Sagar Institute of Research and Technology Pharmacy | Maheshwari R.K.,A And G Pharmaceutical, Inc. | Jain S.,Sagar Institute of Research and Technology Pharmacy
Pakistan Journal of Pharmaceutical Sciences | Year: 2013

A novel, eco friendly, accurate, sensitive, economic and safe spectrophotometric method was developed by application of mixed hydrotropy using 2 M sodium acetate, 8 M urea, 2 M niacinamide and 2 M sodium benzoate solution (25:25:25:25% V/V) as hydrotropic agent, for the solubalizing of poorly water-soluble Furazolidone (FZ) (solubility:- 3.64e-01 mg/mL in water). There were more than 32 times enhancements in the solubility of FZ were found in mixed hydrotropic solution as compared to solubilities in distilled water. FZ shows maximum absorbance at 360 nm where sodium acetate, urea, niacinamide, sodium benzoate and other tablets excipients did not show any absorbance above 300 nm, and thus no interference in the estimation was seen. FZ was obeyed Beer's law in the concentration range of 10 to 50 μg/ml (r2=0.9992) in mixed hydrotropic solvent with mean recovery ranging from 97.32% to 98.9%. Proposed method is new, simple, economic, safe, rapid, accurate and reproducible and was validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values.


Mishra R.,Mahatma Jyoti Rao Phoole University | Jain S.,Sagar Institute of Research and Technology Pharmacy
Pharmacologyonline | Year: 2013

Due to the ever increasing resistance to antibiotics by the microbes, new molecules need to be evaluated for antimicrobial efficacy. Twenty new chalcone derivatives were tested for their ability as antimicrobial compounds. The minimum inhibition concentration of the compounds was evaluated against E. coli, P. aeruginosa, B. subtilis and S. aureus by serial dilution technique. All the tested compounds were found to be to possess MIC values between 50-6.25 μg/mL and the most potent compounds were found to contain a substitution at the para position of ring B. The study led to the conclusion that chalcones with heterocyclic ring A posses the potential to be a promising lead molecule for newer antimicrobial agents.


Mishra R.,Mahatma Jyoti Rao Phoole University | Jain S.,Sagar Institute of Research and Technology Pharmacy
Pharmacologyonline | Year: 2013

Twenty thiazolyl chalcones were tested for their ability to inhibit the growth of Plasmodium facliparum in vitro by using the candle jar method for antimalarial assay. The percent inhibiton of parasitemia was calculated from blood smears stained with geimsa stain. The best compounds had chloro or methoxy group in the para position of the phenyl ring B of the molecule.


Jain R.,Suresh Gyan Vihar University | Jain N.,Sagar Institute of Research and Technology Pharmacy | Jain D.K.,Sagar Institute of Research and Technology Pharmacy | Jain S.K.,Sagar Institute of Research and Technology Pharmacy
Advanced Pharmaceutical Bulletin | Year: 2013

Purpose: Analysis of drug utilized the organic solvent which are costlier, toxic and causing environment pollution. Hydrotropic solution may be a proper choice to preclude the use of organic solvents so that a simple, accurate, novel, safe and precise method has been developed for estimation of poorly water soluble drug Entacapone (Water Solubility-7.97e-02 g/l). Methods: Solubility of entacapone is increased by using 8M Urea as hydrotropic agent. There was more than 67 fold solubility enhanced in hydrotropic solution as compare with distilled water. The entacapone (ENT) shows the maximum absorbance at 378 nm. At this wavelength hydrotropic agent and other tablet excipients do not shows any significant interference in the spectrophotometric assay. Results: The developed method was found to be linear in the range of 4-20 μg/ml with correlation coefficient (r2) of 0.9998. The mean percent label claims of tablets of ENT in tablet dosage form estimated by the proposed method were found to be 99.17±0.63. The developed methods were validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. Conclusion: As hydrotropic agent used in the proposed method so this method is Ecofriendly and it can be used in routine quantitative analysis of drug in bulk drug and dosage form in industries. © 2013 by Tabriz University of Medical Sciences.


Dave V.,Banasthali University | Kushwaha K.,Banasthali University | Yadav R.B.,Banasthali University | Agrawal U.,Sagar Institute of Research and Technology Pharmacy
Journal of Microencapsulation | Year: 2017

The present study was designed to investigate the solubility and penetrability of norfloxacin after the topical application of developed lipid–polymer hybrid nanoparticle (LPN) formulation. The core shell of the LPNs formulation was composed of poly (lactic-co-glycolic acid) that is highly lipophilic in nature, thus control the release of drug. The developed formulations were characterised for size, shape (transmission electron microscopy [TEM], scanning electron microscopy [SEM], and atomic force microscopy), entrapment efficiency, Fourier transform infra-red (FTIR) spectroscopy, differential scanning calorimetry (DSC) and thermo gravimetric analysis (TGA). Moreover, in vitro skin permeation studies were performed to determine release profile of the drug. Norfloxacin loaded nanoparticles retained there antimicrobial efficacy against Staphylococcus aureus and Pseudomonas aeruginosa. Stability study was suggested that the suitable storage condition should be at 4 ± 2 °C/60 ± 5% RH for the LPNs. Therefore, these nanoparticles showed a safe and effective long-lasting approach for long treatment of bacterial infections due to burn. © 2017 Informa UK Limited, trading as Taylor & Francis Group.


Gupta B.P.,Sagar Institute of Research and Technology Pharmacy | Thakur N.,Sagar Institute of Research and Technology Pharmacy | Jain N.P.,Sagar Institute of Research and Technology Pharmacy | Banweer J.,Sagar Institute of Research and Technology Pharmacy | Jain S.,Sagar Institute of Research and Technology Pharmacy
Journal of Pharmacy and Pharmaceutical Sciences | Year: 2010

Conventional drug delivery systems have slight control over their drug release and almost no control over the effective concentration at the target site. This kind of dosing pattern may result in constantly changing, unpredictable plasma concentrations. Drugs can be delivered in a controlled pattern over a long period of time by the controlled or modified release drug delivery systems. They include dosage forms for oral and transdermal administration as well as injectable and implantable systems. For most of drugs, oral route remains as the most acceptable route of administration. Certain molecules may have low oral bioavailability because of solubility or permeability limitations. Development of an extended release dosage form also requires reasonable absorption throughout the gastro-intestinal tract (GIT). Among the available techniques to improve the bioavailability of these drugs fabrication of osmotic drug delivery system is the most appropriate one. Osmotic drug delivery systems release the drug with the zero order kinetics which does not depend on the initial concentration and the physiological factors of GIT. This review brings out new technologies, fabrication and recent clinical research in osmotic drug delivery.


Garg B.,Sagar Institute of Research and Technology Pharmacy | Srivastava N.M.,Gandhi Medical College | Srivastava S.,P.A. College
Der Pharmacia Lettre | Year: 2015

Hyperlipidemia is the highest risk factor of coronary heart disease. Herbal treatment for hyperlipidemia has no side effects and is relatively cheap and locally available, Bougainvillea glabra was selected and the present study on anti-hyperlipidemic activity of extract of Bougainvillea glabra leaves against triton induced hyperlipidemia in rats. Ethanolic extract, aqueous extract, chloroform fraction of ethanolic extract and ethylacetate fraction of ethanolic extract administered at different doses to the triton induced hyperlipidemic rats. Bougainvillea glabra has shown a significant decrease in the levels of serum cholesterol, triglyceride, LDL and significant increase in the level of serum HDL.


Sharma M.,Rajiv Gandhi Technical University | Nautiyal P.,Rajiv Gandhi Technical University | Jain S.,Sagar Institute of Research and Technology Pharmacy | Jain D.,Rajiv Gandhi Technical University
Journal of AOAC International | Year: 2010

Combination therapy with acyclovir and zidovudine is used for the treatment of herpes-infected immunocompromised patients. In the view of the optimal drug concentrations (minimum effective concentrations) for viral suppression and avoidance of drug toxicity, monitoring of drug levels has been considered essential to determine drug concentrations in plasma after administration of a dose of acyclovir and zidovudine. A simple, precise, and rapid RP-HPLC method has been developed for this purpose. Chromatographic separation was performed using methanol-water (50 + 50, v/v), pH 2.5 adjusted with orthophosphoric acid, as an isocratic mobile phase at a flow rate of 0.8 mL/min with an Inertsil ODS (C18) column (5 |μm particle size, 250 × 4.60 mm id). Detection was carried out using a UV photo diode array detector at 258 nm. The plasma samples were prepared by a protein precipitation method. The retention time for acyclovir and zidovudine was 3.5 ± 0.2 and 6.2 ± 0.3 min, respectively. The method was linear in the range of 200-1800 and 400-3600 ng/mL with LOQ of 200 ng (SD = ±1.4) and 400 ng (SD = ±0.9) for zidovudine and acyclovir, respectively, in plasma. The mean accuracy was 98.0 and 96.4%, with average extraction recovery of 64.8 ± 2.1 and77.5 ± 1.7% for lower nominal concentrations of acyclovir and zidovudine, respectively.


Jain N.,Sagar Institute of Research and Technology Pharmacy | Jain R.,Sagar Institute of Research and Technology Pharmacy | Jain D.K.,Truba Institute of Pharmacy | Chandel H.S.,Truba Institute of Pharmacy
Natural Product Research | Year: 2010

The aim of the present study was to assess the wound-healing activity of ethanolic extracts of Acorus calamus leaves. A wound was induced by an excision- and incision-based wound model in rats of either sex. The mature green leaves of A. calamus were collected and authenticated. Extractions of dried leaves were carried out with 80% ethanol in a soxhlet apparatus. For wound-healing activity, the extracts were applied topically once daily in conc. of 40% w/w and 20% w/w in the form of ointment and compared with a standard drug (povidion-iodine). The healing of the wound was assessed by the rate of wound closure, period of epithelialisation, tensile strength and weight of the granulation tissue, hydroxyproline content and histopathology of the granulation tissue. The ethanolic extract of A. calamus promoted wound-healing activity significantly in both the wound models studied. The histological study of the granulation tissue with 20% A. calamus extract ointment-treated animals showed a larger number of inflammatory cells and lesser collagen when compared with the 40% A. calamus extract ointment-treated animals. However, this was better than the control group of animals. Enhanced wound contraction, decreased epithelialisation time, increased hydroxyproline content and histological characteristics suggest that A. calamus extract may have therapeutic benefits in wound healing. © 2010 Taylor & Francis.

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