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Jain D.K.,Truba Institute of Pharmacy | Patel P.,Truba Institute of Pharmacy | Khan A.S.,P.A. College | Jain N.,Sagar Institute of Research and Technology Pharmacy
International Journal of ChemTech Research | Year: 2011

A simple, precise, reliable, rapid and reproducible reversed-phase high performance liquid chromatography method was developed and validated for the simultaneous estimation of Atenolol (ATL) and Lercanidipine Hydrochloride (LER) present in tablet dosage forms. Chromatographic separation achieved isocratically on Luna C18 column (5 μm, 150mm x 4.60mm) and ACN/phosphate buffer (60:40, v/v, pH 3.6) as mobile phase, at a flow rate of 0.5ml/min. Detection was carried out at 235 nm. Linearity for ATL and LER were in the range of 50-250 μg/ml and 10-50 μg/ml respectively. Parameters such as linearity, precision, accuracy, recovery, specificity and ruggedness are studied as reported in the ICH guidelines. The retention times for ATL and LER was found to be 2.27 and 5.97 min respectively. The mean recoveries obtained for ATL and LER were 99.85±0.16 and 99.47±0.32% respectively and RSD was less than 2. The correlation coefficients for all components are close to 1. The relative standard deviations for three replicate measurements in three concentrations of samples in tablets are always less than 2%. Developed method was found to be accurate, precise, selective and rapid for simultaneous estimation of ATL and LER in tablets.


Sharma M.,Rajiv Gandhi Technical University | Nautiyal P.,Rajiv Gandhi Technical University | Jain S.,Sagar Institute of Research and Technology Pharmacy | Jain D.,Rajiv Gandhi Technical University
Journal of AOAC International | Year: 2010

Combination therapy with acyclovir and zidovudine is used for the treatment of herpes-infected immunocompromised patients. In the view of the optimal drug concentrations (minimum effective concentrations) for viral suppression and avoidance of drug toxicity, monitoring of drug levels has been considered essential to determine drug concentrations in plasma after administration of a dose of acyclovir and zidovudine. A simple, precise, and rapid RP-HPLC method has been developed for this purpose. Chromatographic separation was performed using methanol-water (50 + 50, v/v), pH 2.5 adjusted with orthophosphoric acid, as an isocratic mobile phase at a flow rate of 0.8 mL/min with an Inertsil ODS (C18) column (5 |μm particle size, 250 × 4.60 mm id). Detection was carried out using a UV photo diode array detector at 258 nm. The plasma samples were prepared by a protein precipitation method. The retention time for acyclovir and zidovudine was 3.5 ± 0.2 and 6.2 ± 0.3 min, respectively. The method was linear in the range of 200-1800 and 400-3600 ng/mL with LOQ of 200 ng (SD = ±1.4) and 400 ng (SD = ±0.9) for zidovudine and acyclovir, respectively, in plasma. The mean accuracy was 98.0 and 96.4%, with average extraction recovery of 64.8 ± 2.1 and77.5 ± 1.7% for lower nominal concentrations of acyclovir and zidovudine, respectively.


Jain N.,Mahatma Jyoti Rao Phoole University | Singhal S.,Mahatma Jyoti Rao Phoole University | Kumar Jain S.,Sagar Institute of Research and Technology Pharmacy
Asian Journal of Pharmaceutical and Clinical Research | Year: 2013

Objective: Stilbene based compounds are widely represented in nature and have become particular interest because of their wide range of biological activities. The aim of research work was to synthesize many synthetic compounds using stilbene as the essential pharmacophore. Methods: A series of novel P-(substituted phenyl) - 2 (substituted phenyl) Ethene derivatives (1a-1q) have been synthesized by the witting reactions i.e. Substituted benzyl chloride reacted with the phosphonium chloride salt give the P substituted benzyl (chloro) triphenyl phosporane (1) which on further reaction with substituted benzaldehyde yielded the corresponding 1a-1q. Results: The yield of synthesized compound was found to be in the range of 60-85%.Synthesized compounds were characterized by their physiochemical properties like solubility, melting point, IR spectroscopy, 1H NMR spectroscopy, Fab MASS and elemental analysis and result of analysis confirm the structure of synthesize compound. Conclusion: This procedure appears to be a promising and conceptually straightforward route for the synthesis of various Cis-stilbenes. The synthesis and characterization of other phenyl substituted compounds using this method are under investigation, and will be reported in due course.


Jain N.,Sagar Institute of Research and Technology Pharmacy | Jain R.,Sagar Institute of Research and Technology Pharmacy | Thakur N.,Sagar Institute of Research and Technology Pharmacy | Gupta B.P.,Sagar Institute of Research and Technology Pharmacy | And 3 more authors.
Asian Journal of Pharmaceutical and Clinical Research | Year: 2010

Nanotechnology is relatively new, and although the full scope of contributions of these technological advances in the field of human health care remains unexplored, recent advances suggest that nanotechnology will have a profound impact on disease prevention, diagnosis, and treatment. Nanotechnology based delivery system would allow faster drug absorption, controlled dosage release into the human body and would have other unique properties of minimizing side-effects by eliminating requirement of co-solvent as used in conventional dosage form. Further, drugs that have side-effects due to triggering an immune system response can be wrapped in nanoparticle coating and prevent immune system from recognizing and reacting to a foreign substance. It is an ideal targeting system should have long circulating time, it should be present at appropriate concentrations at the target site, and it should not lose its activity or therapeutic efficacy while in circulation.


Jain R.,Suresh Gyan Vihar University | Jain V.,Sagar Institute of Research and Technology Pharmacy | Jain N.,Sagar Institute of Research and Technology Pharmacy | Jain D.K.,Truba Institute of Pharmacy | Jain S.,Sagar Institute of Research and Technology Pharmacy
Journal of Applied Pharmaceutical Science | Year: 2012

The present work describes a novel, accurate, sensitive and economic safe spectrophotometric method was developed by application of hydrotropy, using 8 M Urea solution as hydrotropic solubilizing agent, for the quantitative determination of poorly watersoluble lomefloxacin HCl in tablet dosage form. There were more than 43 times enhancements in the solubility of lomefloxacin HCl increases in hydrotropic solution as compared to solubilities in distilled water. Lomefloxacin HCl shows maximum absorbance at 281 nm. Urea and other tablets excipents did not show any absorbance above 230 nm, and thus no interference in the estimation was seen. Lomefloxacin HCl was obeyed Beer,s law in the concentration range of 5 to 25μg/ml (r2= 0.9998) in hydrotropic solvent with mean recovery ranging from 98.03±0.65 to 98.59±0.32%. Proposed method is new, simple, economic, safe, rapid, accurate and reproducible. The developed methods were validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. The method can be used for routine analysis in both research laboratories, and pharmaceutical and chemical industries to analyze the drugs without the use of organic solvents thus make the environment eco-friendly.

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