Saga Prefectural Livestock Experiment Station

Takeo, Japan

Saga Prefectural Livestock Experiment Station

Takeo, Japan

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Suzuki K.,Japan National Agriculture and Food Research Organization | Waki M.,Japan National Agriculture and Food Research Organization | Yasuda T.,Japan National Agriculture and Food Research Organization | Fukumoto Y.,Japan National Agriculture and Food Research Organization | And 5 more authors.
Bioresource Technology | Year: 2010

Changes in swine wastewater chemical features during an activated sludge treatment process were surveyed on 11 farms, and analyzed with non-biodegradable elements, i.e., phosphorus (P), copper (Cu), and zinc (Zn). In piggery wastewater, they were linearly correlated with suspended solid (SS) concentrations and the major portion was in solid fractions. After the pretreatment step, they were removed, with 80% for total P, 85% for total Cu, and 84% for total Zn. After the activated sludge process, total P, Cu, and Zn were then removed at 83%, 96%, and 95%, respectively. Removing SS thoroughly at each step was shown to be the most important factor in preventing outflow of these elements, since there are linear correlations or a positive relationship between the removal of SS concentrations and their removal in solid form. Most of the P, Cu, and Zn in activated sludge effluent was in soluble form, and the concentrations of Cu and Zn in the effluent were low enough, while further P removal might be required. © 2010 Elsevier Ltd.


Yamagishi T.,Japan National Institute of Advanced Industrial Science and Technology | Takeuchi M.,Japan National Institute of Advanced Industrial Science and Technology | Wakiya Y.,Saga Prefectural Livestock Experiment Station | Waki M.,Japan National Agriculture and Food Research Organization
Water Science and Technology | Year: 2013

Anaerobic ammonium oxidation (anammox) is a novel biological nitrogen removal process that oxidizes NH4 + to N2 with NO2 - as an electron acceptor. The purpose of this study was to examine the potential activity and characteristics of anammox in a conventional swine wastewater treatment facility, which uses an activated sludge system consisting of three cascade aeration tanks equipped with ceramic support material. Anammox activity was estimated by a 15N tracer assay method and was detected in all the sludge and biofilm samples in each aeration tank. Biofilm taken from the third aeration tank, in which the dissolved oxygen concentration was 7.5 mg/L and the wastewater included a high concentration of NO3 -, showed by far the highest anammox activity. A clone library analysis showed the existence of anammox bacteria closely related to 'Candidatus Jettenia asiatica' and 'Ca. Brocadia caroliniensis'. The optimum conditions for anammox activity were a pH of 6.7-7.2, a temperature of 35°C, a NO2 - concentration of 10 mmol/L or less, and an NH 4 + concentration of 32 mmol/L or less. © IWA Publishing 2013.


Yamashita T.,Japan National Agriculture and Food Research Organization | Yamamoto-Ikemoto R.,Kanazawa University | Yokoyama H.,Japan National Agriculture and Food Research Organization | Kawahara H.,Saga Prefectural Livestock Experiment Station | And 2 more authors.
Animal Science Journal | Year: 2015

Mitigation of nitrous oxide (N2O) emission from swine wastewater treatment was demonstrated in an aerobic bioreactor packed with carbon fibers (CF reactor). The CF reactor had a demonstrated advantage in mitigating N2O emission and avoiding NOx (NO3+NO2) accumulation. The N2O emission factor was 0.0003g N2O-N/gTN-load in the CF bioreactor compared to 0.03gN2O-N/gTN-load in an activated sludge reactor (AS reactor). N2O and CH4 emissions from the CF reactor were 42g-CO2 eq/m3/day, while those from the AS reactor were 725g-CO2 eq/m3/day. The dissolved inorganic nitrogen (DIN) in the CF reactor removed an average of 156mg/L of the NH4-N, and accumulated an average of 14mg/L of the NO3-N. In contrast, the DIN in the AS reactor removed an average 144mg/L of the NH4-N and accumulated an average 183mg/L of the NO3-N. NO2-N was almost undetectable in both reactors. © 2014 Japanese Society of Animal Science.


Takasuga A.,National Livestock Breeding Center | Takasuga A.,Shirakawa Institute of Animal Genetics Livestock Technology Association | Takasuga A.,Itoham Foods Inc. | Sato K.,Forestry and Fisheries Research Center | And 21 more authors.
PLoS Genetics | Year: 2015

Recessive skeletal dysplasia, characterized by joint- and/or hip bone-enlargement, was mapped within the critical region for a major quantitative trait locus (QTL) influencing carcass weight; previously named CW-3 in Japanese Black cattle. The risk allele was on the same chromosome as the Q allele that increases carcass weight. Phenotypic characterization revealed that the risk allele causes disproportional tall stature and bone size that increases carcass weight in heterozygous individuals but causes disproportionately narrow chest width in homozygotes. A non-synonymous variant of FGD3 was identified as a positional candidate quantitative trait nucleotide (QTN) and the corresponding mutant protein showed reduced activity as a guanine nucleotide exchange factor for Cdc42. FGD3 is expressed in the growth plate cartilage of femurs from bovine and mouse. Thus, loss of FDG3 activity may lead to subsequent loss of Cdc42 function. This would be consistent with the columnar disorganization of proliferating chondrocytes in chondrocyte-specific inactivated Cdc42 mutant mice. This is the first report showing association of FGD3 with skeletal dysplasia. © 2015 Takasuga et al.


PubMed | Nagasaki Prefectural Beef Cattle Improvement Center, Gifu Prefectural Livestock Research Institute, Tottori Animal Husbandry Experiment Station, National Livestock Breeding Center and 10 more.
Type: Journal Article | Journal: PLoS genetics | Year: 2015

Recessive skeletal dysplasia, characterized by joint- and/or hip bone-enlargement, was mapped within the critical region for a major quantitative trait locus (QTL) influencing carcass weight; previously named CW-3 in Japanese Black cattle. The risk allele was on the same chromosome as the Q allele that increases carcass weight. Phenotypic characterization revealed that the risk allele causes disproportional tall stature and bone size that increases carcass weight in heterozygous individuals but causes disproportionately narrow chest width in homozygotes. A non-synonymous variant of FGD3 was identified as a positional candidate quantitative trait nucleotide (QTN) and the corresponding mutant protein showed reduced activity as a guanine nucleotide exchange factor for Cdc42. FGD3 is expressed in the growth plate cartilage of femurs from bovine and mouse. Thus, loss of FDG3 activity may lead to subsequent loss of Cdc42 function. This would be consistent with the columnar disorganization of proliferating chondrocytes in chondrocyte-specific inactivated Cdc42 mutant mice. This is the first report showing association of FGD3 with skeletal dysplasia.


Takuma T.,Yamaguchi University | Otsubo T.,Saga Prefectural Livestock Experiment Station | Kurokawa Y.,Saga Prefectural Livestock Experiment Station | Ichimaru H.,Saga Prefectural Livestock Experiment Station | Otoi T.,Yamaguchi University
Reproduction in Domestic Animals | Year: 2010

Contents: We investigated the effects of twice-weekly follicular punctures of ovaries with or without corpus luteum (CL) on follicular and luteal dynamics. A cross-over design was used, with each cow (seven Japanese Black beef cows) being assigned to one of the three groups at 2-month intervals. Follicular punctures were performed twice weekly for three consecutive weeks until day 20 (oestrus = day 0). All visible follicles (diameter >3 mm) in the ovaries bearing CL (ipsilateral group) or those in the contralateral ovaries (contralateral group) were aspirated. As a control, all visible follicles in both ovaries were aspirated (bilateral group). Follicular development, CL formation and progesterone concentrations in each cow were monitored from days 0 to 30. Follicular growth profiles in the punctured ovaries during/after puncture treatment were similar, irrespective of the presence of follicles in the unpunctured ovary and the CL in the punctured or unpunctured ovaries. After puncture, two cows (28.6%) each in the ipsilateral and bilateral groups did not exhibit behavioural oestrus until day 30, whereas all cows in the contralateral group exhibited oestrus. CL growth and increase in progesterone concentrations after the last follicular puncture in the bilateral group were delayed when compared with those in the ipsilateral group. Our results indicate that the presence of follicles in the unpunctured ovary and the CL in the punctured or unpunctured ovaries does not significantly influence follicular growth in punctured ovaries during/after puncture treatment. However, follicular puncture in ovaries bearing CL may disturb or delay oestrus occurrence after treatment. © 2008 The Authors. Journal compilation. © 2008 Blackwell Verlag.


Takuma T.,Yamaguchi University | Sakai S.,Saga Prefectural Livestock Experiment Station | Ezoe D.,Saga Prefectural Livestock Experiment Station | Ichimaru H.,Saga Prefectural Livestock Experiment Station | And 4 more authors.
Journal of Reproduction and Development | Year: 2010

The aim of the present study was to determine whether the season (hot and cool) and reproductive phase (pregnant and non-pregnant) of the cow affect follicular recruitment and oocyte development. Follicular oocytes were aspirated from Japanese black cows by the ovum pick-up (OPU) method, which was performed 2 to 6 times within 1.5 months in pregnant cows and 2 to 4 times within 2 months in non-pregnant cows, during the hot (July to September) and cool (October to November) seasons. After follicular aspiration, the number and morphology of cumulus-oocyte complexes (COCs) and the developmental competence of oocytes after in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro culture were evaluated. The quality of aspirated COCs did not differ between the hot and cool seasons, irrespective of the reproductive phase of the donor cows. In the pregnant cows, the season did not affect follicular recruitment, early embryonic development or the quality of embryos. In the non-pregnant cows, however, the mean number of aspirated follicles and collected oocytes decreased during the hot season as compared with the cool season. When the data for the 2 seasons were combined to assess the effects of reproductive phase on oocyte development, the total proportions of cleavage, development into blastocysts and freezable embryos were higher for embryos obtained from pregnant cows (P<0.05) than those obtained from non-pregnant cows. In conclusion, the season did not have any apparent effects on the quality of aspirated COCs and the developmental competence of oocytes after IVM-IVF, but it may affect follicular recruitment in non-pregnant cows. Moreover, the reproductive phase may influence the developmental competence of the recovered oocytes. © 2010 by the Society for Reproduction and Development.


Fukuda O.,Japan National Institute of Advanced Industrial Science and Technology | Nabeoka N.,Japan National Institute of Advanced Industrial Science and Technology | Miyajima T.,Saga Prefectural Livestock Experiment Station
2012 19th International Conference on Systems, Signals and Image Processing, IWSSIP 2012 | Year: 2012

In the present paper, we propose a new method to estimate the beef marbling standard (BMS) number by dynamic ultrasound imaging of live beef cattle. The proposed method consists of four processes: time series texture analysis, extraction of dynamic features, principal component analysis, and estimation of BMS number by a neural network. Highly accurate estimation is expected by frequency analysis of time series texture features extracted from dynamic ultrasound imaging and averaging the texture features. In addition, a neural network model can flexibly represent a non-linear mapping relationship between image features and BMS number. The correlation coefficient between the actual BMS number and the BMS number estimated using dynamic image features by the leave-one-out method is r = 0.75(P < 0.01), and the mean estimation error is 1.09. These results suggest that dynamic image features extracted from dynamic ultrasound images have the potential to accurately estimate the BMS number. © 2012 Institute of Telecommunica.


Aono A.,Kagoshima University | Nagatomo H.,Kagoshima University | Nagatomo H.,Hokkaido University | Takuma T.,Saga Prefectural Livestock Experiment Station | And 11 more authors.
Theriogenology | Year: 2013

The objective was to determine if immature bovine oocytes with cumulus cells at the germinal vesicle (GV) stage could be vitrified by aluminum sheets (AS; pieces of sheet-like aluminum foil). Cleavage rates in fertilized oocytes previously vitrified by the AS procedure were higher than those vitrified by a nylon-mesh holder (NM) procedure (89.3 ± 2.1% vs. 65.0 ± 3.7%). Cleaved embryos derived from the AS but not from the NM procedures developed to blastocysts. Furthermore, to investigate the effects of vitrifying GV oocytes on cytoplasmic structure and on the ability to undergo cytoplasmic changes, the intracellular phospholipid membrane (IM) was stained with the lipophilic fluorescent dye, 3,3'-dioctadecyloxa-carbocyanine perchlorate. After vitrification by AS, the IM remained intact relative to that of oocytes vitrified by NM. During in vitro maturation, reorganization of the IM was also undamaged in oocytes vitrified by AS before oocyte maturation, and the IM within oocytes vitrified by the NM procedure was evidently impaired. Finally, vitrification (AS) was used for GV oocytes collected using the ovum pick-up method. A bull calf was born after in vitro production and subsequent embryo transfer. The vitrification techniques described herein should facilitate generation of viable in vitro production bovine blastocysts using oocytes recovered using the ovum pick-up method. © 2013 Elsevier Inc.


Nagatomo H.,Hokkaido University | Kagawa S.,Hokkaido University | Kishi Y.,Tokyo University of Agriculture | Takuma T.,Saga Prefectural Livestock Experiment Station | And 7 more authors.
Biology of Reproduction | Year: 2013

Mice and cattle use distinct pathways for the first cell segregation into inner cell mass (ICM) and trophectoderm (TE) lineages at the blastocyst stage. However, limited knowledge is available regarding the reliable transcriptional networks that orchestrate the complex developmental processes at this stage in nonrodent species. In order to elucidate the site-dominant transcriptomic properties of bovine blastocysts, we separated cell samples into the ICM and TE using both mechanical and chemical methods and performed in silico prescreening for candidate genes that were site-dominantly expressed in bovine blastocysts. We further performed quantitative real-time PCR and in situ hybridization using the site-specific cell samples. As a result, we identified seven ICM-dominant genes and five TEdominant genes not found in earlier studies. Our findings provide novel insights into the mechanism of cell-fate specification in the pre-implantation bovine embryo. © 2013 by the Society for the Study of Reproduction, Inc.

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