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Shibu B.S.,Karpagam University | Shibu B.S.,SAFI Institute of Advanced Study | Servin Wesley P.,Karpagam University | Servin Wesley P.,SAFI Institute of Advanced Study | And 3 more authors.
Indian Journal of Experimental Biology | Year: 2014

The seeds of C. nervosa and E. pseudoclavicaulis were germinated asymbiotically on Knudson C (KC) and Schenk and Hildebrandt basal medium (SH). Growth regulators such as 2,4-Dichlorophenoxyacetic acid (2,4-D) individually and in combinations with benzyladenine (BA) and kinetin were used for callus induction from the protocorm like bodies. Coelogyne nervosa showed maximum (90%) callus induction in Knudson C medium supplemented with 2,4-D (2.26 μM) and Eria pseudoclavicaulis showed 60% callus induction in Schenk and Hildebrandt medium supplemented with 2,4-D (2.26 μM). Calli developed a route of production of protocorm-like bodies and eventually developed into plantlets on transfer to growth regulator free half strength basal medium. The well rooted plants were hardened successfully in the potting mixture containing coconut husk, charcoal, and brick pieces in the ratio 2:1:1.

Ragi T.P.,SAFI Institute of Advanced Study | Sahaya Shibu B.,SAFI Institute of Advanced Study
Asia-Pacific Journal of Molecular Biology and Biotechnology | Year: 2014

An efficient protocol for in vitro propagation of Boerhavia diffusa L. has been standardized using nodal and leaf explants. Experiments were performed to examine the effect of various growth regulators on callus induction, multiple shoot formation and complete plantlet regeneration. Complete success in callus induction with an average of 3 ± 0.66 shoots were observed on Murashige and Skoog (MS) basal medium supplemented with 2,4-D (9.03 μM). Multiple shoot proliferation (4 ± 0.89) was achieved on MS basal medium supplemented with kinetin (4.64 μM). Well rooted plantlets were hardened and acclimatized in the field and achieved a 65 % survival rate. © 2014, University of Malaya. All rights reserved.

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