Faiz Arshad M.,Shaqra University |
Kumar S.,Jamia Hamdard University |
Al Rohaimi A.H.,Shaqra University |
Hassan A.A.,Shaqra University |
And 3 more authors.
Medicinal Chemistry Research | Year: 2015
A series of dihalosubstituted thiocarbamide derivatives (5a-e, 6a-e, 7a-e, and 8a-e) were synthesized as antitubercular agents. The structures of dihalosubstituted thiocarbamide derivatives were established on the basis of IR, 1H, 13C NMR, and mass spectral data. All the compounds were tested in vitro for antimycobacterial activity against Mycobacterium tuberculosis (ATCC-25177) by well-diffusion method and MIC by serial dilution method. Results of the antitubercular screening revealed that some of the compounds showed moderate to good antitubercular activity. Of all the compounds tested, two derivatives viz., 8d and 8e expressed MIC of 25 μg/ml, and one compound 7e showed MIC of 12.5 μg/ml against M. tuberculosis (ATCC-25177). Graphical Abstract: [Figure not available: see fulltext.] © 2014 Springer Science+Business Media New York.
Saif M.,Ain Shams University |
Shebl M.,Ain Shams University |
Nabeel A.I.,Ain Shams University |
Shokry R.,Ain Shams University |
And 5 more authors.
Sensors and Actuators, B: Chemical | Year: 2015
Abstract Lanthanide doped inorganic nano-phosphor embedded into silica matrix have shown great potential in novel chemical and biological sensor development due to their pure, intense and stable photoluminescence (PL) properties. However, their potential applications in forensic science, especially in latent fingerprint detection in particular, were still unexplored. In this study, we have succeeded in production of high PL, photo-stable and non-toxic x mol% Eu3+:Y2Ti2O7 embedded into silica matrix powder (x mol% Eu3+/Y2Ti2O7 where x = 1.0, 2.0, 4.0 6.0, 10, 15 and 30) using sol-gel method followed by heat treatment. The crystal, morphological, optical and PL properties of the nano-phosphor powders were characterized by X-ray diffraction (XRD), transmission electron microscope (TEM), energy dispersive spectroscopic (EDS), FT-IR and photoluminescence (PL) spectroscopy. Judd-Ofelt (J-O) theory was applied to a series of x mol% Eu3+:Y2Ti2O7 embedded into silica matrix based on their emission spectra. The results of in vivo toxicity test indicated that the prepared nano-phosphor embedded into silica matrix is non-toxic (LD50) for 15 mol% Eu3+ doped powder = 6.26 g/kg). The prepared nano-phosphor was successfully developing in latent fingerprint from various forensic relevant materials, including non-porous and porous surfaces. © 2015 Elsevier B.V.
El-Emshaty H.M.,Mansoura University |
Nasif W.A.,Sadat University |
Nasif W.A.,University of Umm Al - Qura |
Mohamed I.E.,Mansoura University
Disease Markers | Year: 2015
The current study was designed to investigate the potential association of serum interleukin-10 and interleukin-12 with HCV infection in chronic liver disease and to evaluate their possible role as new biomarkers in HCC development. Material and Methods. Forty-one patients suffering from chronic liver disease (33 patients harbor HCV infection and 8 are HCV-negative patients) were enrolled in the present study and histopathologically diagnosed into 15 patients with HCC, 16 patients with LC, and 10 patients with liver histology compatible with precirrhotic hepatitis (PCH). Ten patients complaining of cholecystitis were included as nondisease control. Serum levels of IL-10 and IL-12 were measured by enzyme linked immunosorbent assay (ELISA). Results. HCV-infected patients showed elevated expression of IL-10 and IL-12 compared to nondisease controls (P < 0.0001) but there is no significant difference with respect to their expression in HCV-negative patients. Serum IL-10 and IL-12 were elevated significantly with disease progression (P < 0.0001) and a positive correlation coefficient was detected between IL-10, IL-12 (r = 0.785, P < 0.0001), and transaminase values suggesting their possible role in chronic inflammation progression leading to HCC. Conclusion. IL-10 and IL-12 might be involved in chronic inflammation progression leading to HCC and their evaluation could be used as new biomarkers to reflect the degree of inflammation in HCC development. © 2015 Hoda Mohamed El-Emshaty et al.
Sabry M.,Sadat University |
Elkerdasy A.,Sadat University |
Elkerdasy A.,Shaqra University
Journal of Pharmacy and Bioallied Sciences | Year: 2014
Background: In most African and Arabic countries tuberculosis (TB) causes great economic losses in bovine species and constitutes serious zoonotic problem. As the traditional diagnostic method delay the research because of low sensitivity and specificity, a rapid method of diagnosis is of outmost importance. Aim: The study was designed to evaluate the two rapid diagnostic methods of TB in cattle, further to differentiate between infected and bacillus Calmette-Guerin (BCG) vaccinated animals. Materials and Methods: Intradermal tuberculin test was applied to 300 cattle. Of these cattle, 15 cattle were vaccinated from cattle negative to tuberculin test with BCG. Blood samples were taken for lymphocyte separation to apply polymerase chain reaction (PCR) upon and for serum preparation for the enzyme-linked immunosorbent assay (ELISA) application, this blood collected from 65 cattle classified into three groups, viz. positive tuberculin test (35 animals), negative tuberculin test (15 animals), and vaccinated cow with BCG (15 animals). From blood samples lymphocytes were separated and the isolated lymphocytes were subjected to PCR and serum for ELISA application. Blood samples, specimens from lymph nodes and specific tissues were taken for PCR and for cultivation and isolation of Mycobacterium bovis. Results and Conclusions: The results of this study revealed that PCR can be used as rapid efficient and accurate diagnostic test in detection of ruminant TB. Moreover, cattle′s ELISA reading showed higher sensitivity in positive tuberculin animals. However, the differentiations between vaccinated and infected animals not clear by using a single antigen only.
Wehaish F.E.,Mansoura University |
Elseady Y.Y.,Mansoura University |
Saleh R.M.,Mansoura University |
Elshal M.F.,Sadat University
Life Science Journal | Year: 2014
Renal dysfunction is a well-known dose-limiting toxicity of the chemotherapeutic drug Cisplatin (CP). CP was also reported to trigger a degenerative process of endothelium that contributes to vascular abnormalities. We aimed to investigate the molecular and biochemical mechanisms contributing to cisplatin-induced vascular damage by evaluating mRNA expression of genes related to leukocytes adhesion (ICAM-1), inflammation (TNF-α and TNFR-1), oxidative stress (HO-1), and basement membrane molecules (desmin, and nephrin). Endothelial damage was evaluated in aorta sections by immunohistochemical staining with von Willebrand factor (vWF). Rats injected with CP (4 mg/kg) i.p. per week for 4 weeks showed significant deterioration in biomarkers of kidney and heart functions, increased oxidative stress (NO and MDA), and decreased antioxidant enzymes of kidney and heart tissue homogenates. CP-injected rats showed also a prominent increase in mRNA of the studied genes compared with control rats. CP-group also showed a significant decrease in vWf expression in aorta sections in comparison to aorta from healthy rats. In conclusion, our data indicated that cisplatin induces cardiovascular changes in addition to renal dysfunction, and that increased leukocytes adhesion, oxidative stress and inflammation may account for these abnormalities.
Abou-Easa K.,Kafr El Sheikh University |
Abu El-Magd M.,Kafr El Sheikh University |
Tousson E.,Tanta University |
Hassanin A.,Sadat University |
And 2 more authors.
International Journal of Morphology | Year: 2015
Several functional and morphological studies have been conducted on the pineal gland in many mammalian species; however, no published reports are available on the role of pineal gland on the gonadal development before and after eyelids separation in puppies. Therefore, this study aimed to trace the postnatal histo-morphological changes in the pineal gland and gonads of puppies before (2, 10 and 11 days old) and after (25, 35 and 40 days old) eyelids separation in an attempt to investigate the possible role of pineal gland on the gonadal development. In general, the differentiation of pineal cells, interstitial endocrine cells of testes and stromal ovarian cells coincides with the start of eyelids separation in puppies. Histological examination of stained pineal and gonadal slices of puppies after eyelids separation revealed a remarkable differentiation of pinealocytes and testicular interstitial endocrine cells, as well as presence of some evidence of folliculogenesis in ovary. Surprisingly, melatonin receptor (MT1) protein expression levels were significantly increased in the ovaries and testes of puppies after eyelids separation. Moreover, the mRNA and protein expression of AANAT, a ratelimiting enzyme in melatonin biosynthesis, was notably increased in the pineal gland of opened eyes puppies. Our results suggest an increase of melatonin production from the pineal gland of opened eyes puppies and this could play a vital role in the developmental changes observed in the gonads of these puppies. © 2015, Universidad de la Frontera. All rights reserved.
Salem A.M.S.,Central Metallurgical Research and Development Institute |
Harraz F.A.,Central Metallurgical Research and Development Institute |
El-Sheikh S.M.,Central Metallurgical Research and Development Institute |
Hafez H.S.,Sadat University |
And 2 more authors.
RSC Advances | Year: 2015
The electrochemical anodization of a single crystalline silicon in hydrofluoric acid-based solution leads to the formation of porous silicon (PSi) with tunable pore sizes and morphology for a wide range of technological applications. By infiltrating the as-anodized PSi layer with a conducting polymer, new functionalities can be achieved. Herein, we report on the enhancement of the electrical and luminescent properties of a nanohybrid composed of ∼100 nm PSi infiltrated by poly(2-methoxy-5-(2-ethylhexyloxy)-p-phenylenevinylene) (MEH-PPV) via a repeated spin coating technique. Morphological and structural investigations using FE-SEM and XRD on the hybrid nanostructure revealed a successful deposition of MEH-PPV inside the entire porous channels with a low degree of crystallinity. A partial silicon oxide formation was confirmed by FT-IR and XPS measurements. Furthermore, a remarkable increase in the electrical properties was detected by measuring the I-V curves and the electrochemical impedance spectroscopy (EIS). Moreover, a noticeable photoluminescence (PL) spectral enhancement after the MEH-PPV deposition into the PSi was detected. The spectral analyses for the current system indicate the possibility of exciton transfer from PSi to the MEH-PPV polymer. This simple synthetic approach can open new opportunities for the development of hybrid nanostructures of PSi and conducting polymers with potential for optical device applications. © 2015 The Royal Society of Chemistry.
Faiz Arshad M.,Shaqra University |
Siddiqui N.,Jamia Hamdard University |
Elkerdasy A.,Shaqra University |
Elkerdasy A.,Sadat University |
And 2 more authors.
American Journal of Pharmacology and Toxicology | Year: 2014
The present series of compounds were synthesized with the aim to develop newer anticonvulsant agents that are comparatively more efficacious and safer than the currently used anticonvulsant agents. Various thiazolyl coumarins were synthesized by the reaction of 3-(bromoacetyl)-2H-chromen-2-one with different substituted aryl thiourea. The structures of the synthesized compounds were confirmed by spectral data and elemental analyses. Compounds were tested for anticonvulsant activity utilizing Pen Tylenetetra Zoleinduced seizure (PTZ) and Maximal Electroshock Seizure (MES) tests at 30, 100 and 300 mg kg-1 dose level. Neurotoxicity and ethanol potentiation test of the compounds were also assessed at the same dose level. Two compounds of the series 3g and 3j exhibited significant anticonvulsant activity at 30 mg kg-1 dose level with lesser neurotoxicity than the standard drug phenytoin. © 2014 Science Publication.