Elsayed S.,Sadat City University |
Darwish W.,Sadat City University
Bragantia | Year: 2017
Spectral remote sensing offers the potential to provide more information for making better-informed management decisions at the crop canopy level in real time. In contrast, the traditional methods for irrigation management are generally time-consuming, and numerous observations are required to characterize them. The aim of this study was to investigate the suitability of hyperspectral reflectance measurements of remote sensing technique for salinity and water stress condition. For this, the spectral indices of 5 maize cultivars were tested to assess canopy water content (CWC), canopy water mass (CWM), biomass fresh weight (BFW), biomass dry weight (BDW), cob yield (CY), and grain yield (GY) under full irrigation, full irrigation with salinity levels, and the interaction between full irrigation with salinity levels and water stress treatments. The results showed that the 3 water spectral indices (R970 - R900)/(R970 + R900), (R970 - R880)/(R970 + R880), and (R970 - R920)/(R970 + R920) showed close and highly significant associations with the mentioned measured parameters, and coefficients of determination reached up to R2 = 0.73*** in 2013. The model of spectral reflectance index (R970 - R900)/(R970 + R900) of the hyperspectral passive reflectance sensor presented good performance to predict the CY, GY, and CWC compared to CWM, BFW, and BDW under full irrigation with salinity levels and the interaction between full irrigation with salinity levels and water stress treatments. In conclusion, the use of spectral remote sensing may open an avenue in irrigation management for fast, high-throughput assessments of water status, biomass, and yield of maize cultivars under salinity and water stress conditions. © 2017, Instituto Agronomico. All rights reserved.
El Aidy S.,Alimentary Pharmabiotic Center |
El Aidy S.,Sadat City University |
Dinan T.G.,Alimentary Pharmabiotic Center |
Dinan T.G.,University College Cork |
And 2 more authors.
Clinical Therapeutics | Year: 2015
Purpose: It is well established that mammals are so-called super-organisms that coexist with a complex microbiota. Growing evidence points to the delicacy of this host-microbe interplay and how disruptive interventions could have lifelong consequences. The goal of this article was to provide insights into the potential role of the gut microbiota in coordinating the immune-neuroendocrine cross-talk. Methods: Literature from a range of sources, including PubMed, Google Scholar, and MEDLINE, was searched to identify recent reports regarding the impact of the gut microbiota on the host immune and neuroendocrine systems in health and disease. Findings: The immune system and nervous system are in continuous communication to maintain a state of homeostasis. Significant gaps in knowledge remain regarding the effect of the gut microbiota in coordinating the immune-nervous systems dialogue. Recent evidence from experimental animal models found that stimulation of subsets of immune cells by the gut microbiota, and the subsequent cross-talk between the immune cells and enteric neurons, may have a major impact on the host in health and disease. Implications: Data from rodent models, as well as from a few human studies, suggest that the gut microbiota may have a major role in coordinating the communication between the immune and neuroendocrine systems to develop and maintain homeostasis. However, the underlying mechanisms remain unclear. The challenge now is to fully decipher the molecular mechanisms that link the gut microbiota, the immune system, and the neuroendocrine system in a network of communication to eventually translate these findings to the human situation, both in health and disease. © 2015 Elsevier HS Journals, Inc. All rights reserved.
Effects of black pepper (piper nigrum), turmeric powder (curcuma longa) and coriander seeds (coriandrum sativum) and their combinations as feed additives on growth performance, carcass traits, some blood parameters and humoral immune response of broiler chickens
Abou-Elkhair R.,Sadat City University |
Ahmed H.A.,Damanhour University |
Selim S.,University of Helsinki
Asian-Australasian Journal of Animal Sciences | Year: 2014
Different herbs and spices have been used as feed additives for various purposes in poultry production. This study was conducted to assess the effect of feed supplemented with black pepper (Piper nigrum), turmeric powder (Curcuma longa), coriander seeds (Coriandrum sativum) and their combinations on the performance of broilers. A total of 210 (Cobb) one-d-old chicks were divided into seven groups of 30 birds each. The treatments were: a control group received no supplement, 0.5% black pepper (T1), 0.5% turmeric powder (T2), 2% coriander seeds (T3), a mixture of 0.5% black pepper and 0.5% turmeric powder (T4), a mixture of 0.5% black pepper and 2% coriander seed (T5), and a mixture of 0.5% black pepper, 0.5% turmeric powder and 2% coriander seeds (T6). Higher significant values of body weight gain during the whole period of 5 weeks (p<0.001) were observed in broilers on T1, T3, T5, and T6 compared to control. Dietary supplements with T1, T2, T3, and T6 improved the cumulative G:F of broilers during the whole period of 5 weeks (p<0.001) compared with control. The dressing percentage and edible giblets were not influenced by dietary supplements, while higher values of relative weight of the liver (p<0.05) were obtained in T5 and T6 compared to control. The addition of feed supplements in T5 and T6 significantly increased serum total protein and decreased serum glucose, triglycerides and alkaline phosphatase concentrations compared with the control group (p<0.05). Broilers on T6 showed significant decrease in the serum glutamate pyruvate transaminase concentration (p<0.05) compared to control. The broilers having T5 and T6 supplemented feed had relatively greater antibody titre (p<0.001) at 35 d of age than control. It is concluded that dietary supplements with black pepper or coriander seeds or their combinations enhanced the performance and health status of broiler chickens. © 2014 by Asian-Australasian Journal of Animal Sciences.
Aidy S.E.,TI Food and Nutrition |
Aidy S.E.,Alimentary Pharmabiotic Center |
Aidy S.E.,Sadat City University |
van den Bogert B.,TI Food and Nutrition |
And 4 more authors.
Current Opinion in Biotechnology | Year: 2015
The intestinal microbiota plays a profound role in human health and extensive research has been dedicated to identify microbiota aberrations that are associated with disease. Most of this work has been targeting the large intestine and fecal microbiota, while the small intestine microbiota may also have a profound impact on various aspects of the host's physiology, including immune, metabolic and endocrine functions. This review highlights the recent advances made in the study of the human small intestine microbiota. In addition, it describes recent human and animal studies that underpin the importance of this part of the intestine for health of the host organism. © 2014.
Talaat R.M.,Sadat City University |
Mohamed S.F.,Sadat City University |
Bassyouni I.H.,Cairo University |
Raouf A.A.,Menoufia University
Cytokine | Year: 2015
Aim: Imbalance of T-helper-cell (TH) subsets (TH1/TH2/TH17) and regulatory T-cells (Tregs) is suggested to contribute to the pathogenesis of Systemic lupus erythematosus (SLE). Therefore, we evaluated their cytokine secretion profile in SLE patients and their possible association with disease activity. Methods: Sixty SLE patients, 24 rheumatoid arthritis (RA) patients and 24 healthy volunteers were included in this study. Demographic, clinical, disease activity and serological data were prospectively assessed. Plasma cytokines levels of TH1 (IL-12, IFN-γ), TH2 (IL-4, IL-6, IL-10), TH17 (IL-17, IL-23) and Treg (IL-10 and TGF-β) were measured by enzyme linked immunosorbent assays (ELISA). Results: SLE patients were found to have significantly higher levels of IL-17 (. p<. 0.001), IL-6 (. p<. 0.01), IL-12 (. p<. 0.001) and IL-10 (. p<. 0.05) but comparable levels of IL-23 and IL-4 and slight reduction (but statistically insignificant) of TGF-β levels compared to controls. IL-6, IL-10 and IL-17 were significantly increased (. p<. 0.05) with disease activity. The RA group exhibited significantly higher levels of plasma IL-4 (. p<. 0.01), IL-6 (. p<. 0.05), IL-17 (. p<. 0.001), IL-23 (. p<. 0.01) and TGF-β (. p<. 0.5) and lower IFN-γ (. p<. 0.001) and IL-10 (. p<. 0.01) than those of healthy subjects. Conclusion: Our study showed a distinct profile of cytokine imbalance in SLE patients. Reduction in IFN-γ (TH1) and TGF-β1 (Treg) with the elevation in IL-6 and IL-17 (TH17) could imply skewing of T-cells toward TH17 cells. Breaking TH17/Treg balance in peripheral blood may play an important role in the development of SLE and could be responsible for an increased pro-inflammatory response especially in the active form of the disease. © 2015 Elsevier Ltd.
Abdel-Daim M.M.,Suez Canal University |
Abdel-Daim M.M.,Gifu University |
Abd Eldaim M.A.,Sadat City University |
Mahmoud M.M.,Jazan University
Canadian Journal of Physiology and Pharmacology | Year: 2014
Trigonella foenum-graecum L. is enriched with many active ingredients. TFG oil was evaluated for its protective effect against deltamethrin toxicity in rats. Rats of the control group were administered saline. The 2nd group was administered deltamethrin (DLM) orally at a concentration of 15 mg/kg body mass. The 3rd and 4th groups were administered DLM at a concentration of 15 mg/kg body mass and were fed diets containing 2.5% and 5% TFG oil, respectively. DLM intoxication reduced red blood cell and platelet counts, hemoglobin concentration, and hematocrit value while it induced leucocytosis. Furthermore, it increased serum levels of lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, γ-glutamyltransferase, triglycerides, cholesterol, uric acid, urea, and creatinine; increased hepatic, renal, and brain lipid peroxidation; decreased serum acetylcholine esterase level; and decreased hepatic, renal, and brain antioxidant markers' activities. However, TFG oil kept the studied hematological and biochemical parameters within normal ranges. In addition, it prevented lipid peroxidation and oxidative stress induced by DLM intoxication in a dose-dependent manner. Therefore, these results indicated that TFG oil inhibited the toxic effects of DLM on hematological and biochemical parameters as well as oxidative status by its free radical scavenging and potent antioxidant activities, and it appeared to be a promising protective agent against DLM-induced toxicity.
Dweedar H.E.,Sadat City University |
Mahrous H.,Sadat City University |
Ibrahim H.S.,Egyptian Russian University |
Abdel-Aziz H.A.,King Saud University |
Abdel-Aziz H.A.,National Research Center of Egypt
European Journal of Medicinal Chemistry | Year: 2014
The docking studies on CDK2 and GSK-3β inspired us to synthesis a series of indoline-2,3-dione hydrazones 10a-l. Treatment of indoline-2,3-dione derivatives 7a-d with hydrazine gave 3-hydrazonoindolin-2-ones 8a-d which were reacted with the appropriate aldehydes 9a-c to yield 3-substituted- (methylenehydrazono)indolin-2-ones 10a-l. Compounds 10a-l showed a significant anticancer activity against human breast cell line MCF-7. Compounds 10c, f, i exhibited the highest activity almost the same of doxorubicin (IC50 = 6.10 μM) with IC50 = 7.75, 6.75, 6.25 μM, respectively. © 2014 Elsevier Masson SAS. All rights reserved.
Othman A.M.,Sadat City University |
Li S.,University of Miami |
Leblanc R.M.,University of Miami
Analytica Chimica Acta | Year: 2013
Reaction of formaldehyde with amino acids followed by oxidation with hydrogen peroxide to produce a fluorophore Norharman product is well known and was used for the spectrofluorimetric determination of l-tryptophan (Trp). This study aimed to use graphene oxide (GO) to enhance the selectivity and sensitivity of Trp in presence of other amino acids and possible interfering compounds. Different parameters such as pH, temperature, incubation time, and concentrations of formaldehyde, H2O2 and GO were studied to optimize the condition of determination. Experimental data showed that the maximum fluorescence intensity was achieved in pH 7.0-9.0 phosphate buffer mixed with 7-10% (v/v) formaldehyde and 1-2% (v/v) H2O2 as oxidizing agent at 60̊C for 1h. On the basis of calibration curve of various concentrations of Trp in the presence of 20μgmL-1 GO, the lower limit of detection (LOD) of Trp was determined as 0.092nmolmL-1 and the lower limit of quantification (LOQ) was 0.3nmolmL-1. The selectivity of Trp in presence of other amino acids and possible interfering compounds were studied with and without GO. The data obtained after inner filter effect corrections revealed that the selectivity of Trp in presence of amino acids and other possible interfering agents was improved in the range of 76-96%, compared with that in absence of GO. The enhancement of selectivity in the presence of GO indicates that the Trp and other amino acid and possible interfering compounds were adsorbed by GO, and the selective uptaking of Trp-by the reaction with formaldehyde followed by oxidation with H2O2 at 60̊sC with high selectivity and sensitivity was achieved successfully. © 2013 Elsevier B.V.
Hassan H.A.,Sadat City University
Life Science Journal | Year: 2014
27 bacterial isolates were isolated from PCBs contaminated site. A partial 16S rRNA gene sequence revealed that the isolates belonged to the genus Pseudomonas, Bacillus, Staphylococcus, Rhodococcus, Burkholderia, and Pandoraea, all the isolates have the capability to use monochlorinated biphenyl as the sole carbon source, ten of these isolates were belonged to genus Pseudomonas, Burkholderia, Bacillus and Rhodococcus had also the capability to use dichlorinated biphenyl as the sole carbon source and only two strains Burkholderia sp. HA-OP24 and Rhodococcus sp. HA-30 were capable of metabolizing the highly recalcitrant congener 2,4,5,2′,4′,5′-chlorobiphenyl. Two catechol 2,3 dioxygenase genes termed C23O1 and C23O2 were identified and characterized from Burkholderia sp. HA-OP24 and Pseudomonas sp. HA-OP22 respectively. These genes may act as functional genes in the metabolic pathway of the PCBs. These findings may benefit in the bioremediation of PCBs by selecting the vital C23O for the mineralization of PCBS compounds.
Rabey H.E.,King Abdulaziz University |
Rabey H.E.,Sadat City University
Molecular Biology Reports | Year: 2014
The ITS region of the ribosomal RNA genes from two and six-rowed cultivated barley (Hordeum vulgare subsp. distichon and H. v. subsp. hexastichon, respectively), and its two and six-rowed wild relatives (H. v. subsp. spontaneum and H. v. subsp. agriocrithon, respectively) was isolated and sequenced. The entire ITS region is 598 bp in the two-rowed taxa (H. v. subsp. distichon and H. v. subsp. spontaneum ) and 599 bp in the sixrowed ones (agriochriton and hexastichon). The ITS1 is 217 bp in the six-rowed barleys (H. v. subsp. agriochriton and H. v. subsp. hexastichon) and 218 bp in the two-rowed barleys (H. v. subsp. distichon and H. v. subsp. spontaneum). The 5.8S region is 163 bp in all studied H. vulgare taxa. The ITS2 region is 217 bp in the two-rowed barleys ( H. v. subsp. distichon and H. v. subsp. spontaneum) and 219 bp in the six rowed ones (H. v. subsp. hexastichon and H. v. subsp. agriochriton ). The ITS sequence data of the studied taxa and that of three other wild Hordeum species ( H. murinum, H. marinum and H. chilense) were aligned and a phylogeny tree was reconstructed using the Laser-gene Program. H. v. subsp. spontaneum was appeared as the ancestor of all other H. vulgare taxa. © Springer Science+Business Media 2013.