Sabzevar University of Medical science
Sabzevar University of Medical science
Naeemipour M.,Sabzevar University of Medical science
Gazzetta Medica Italiana Archivio per le Scienze Mediche | Year: 2017
BACKGROUND: Role of PGCs for producing transgenic chickens has significantly increased recently as a result of their exclusives capabilities. Primordial germ cells (PGCs) isolation in high numbers is the first step toward transgenic chicken production. MethodS: in the present study we used the main methods to purify chicken pGcs including the isolating of pGcs with ammonium chloride-potassium (acK) buffer, Ficoll and percoll density gradient centrifugation, and using the Magnetic cell sorting (MacS). We compared the methods based on the number of pGcs obtained. reSUltS: our results show that Ficoll density gradient centrifugation method is the preferential method. CONCLUSIONS: There is a demand for a more suitable method for more efficient PGCs isolation. © 2016 EDIZIONI MINERVA MEDICA.
Ghasemzadeh M.R.,Tarbiat Modares University |
Amin B.,Sabzevar University of Medical science |
Mehri S.,Mashhad University of Medical Sciences |
Mirnajafi-Zadeh S.J.,Tarbiat Modares University |
Hosseinzadeh H.,Mashhad University of Medical Sciences
Journal of Ethnopharmacology | Year: 2016
Ethnopharmacological relevance According to traditional medicine, rosemary (Rosmarinus officinalis) has been used in many ailments such as dysmenorrhea, rheumatic pain and stomachache. Aim of the study This work was carried out to evaluate putative anti-allodynic and anti-hyperalgesic effects of Rosmarinus officinalis alcoholic extract and some spinal cord molecular changes on a neuropathic pain model in rats. Materials and methods Peripheral neuropathy was induced by chronic constriction injury (CCI) of sciatic nerve. Rats were treated daily with alcoholic extract of aerial parts of R. officinalis (100, 200, and 400 mg/kg, i.p.), from the day of surgery (day 0) for 14 days. Mechanical allodynia, cold allodynia and heat hyperalgesia were measured on days 0, 3, 5, 7, 10 and 14. Investigations into mechanisms involved measurement of apoptotic factors (bcl-2-like protein (Bax)), cleaved caspases 3 and 9, anti-apoptotic mediator, Bcl2, inflammatory mediators including tumor necrosis factor alpha (TNF-α), inducible isoform of nitric oxide synthase (iNOS), toll like receptor 4 (TLR4), microglial activation marker, ionized calcium-binding adapter molecule 1 (Iba-1) and astroglia activation marker, glial fibrillary acidic protein (GFAP) were measured via western blot on days 7 and 14. Results CCI rats exhibited a marked mechanical allodynia, cold allodynia, and thermal hyperalgesia on days 3, 5, 7, 10, and 14 post-CCI. All three doses of rosemary alcoholic extract were able to attenuate neuropathic behavioral changes as compared with CCI animals that received vehicle. In the vehicle-treated CCI group, a significant increase in levels of Bax, cleaved caspases 3 and 9, Iba1, TNF-α, iNOS and TLR4 levels was detected on both days 7 and 14. Rosemary extract, 400 mg/kg significantly decreased the amounts of mentioned apoptotic, inflammatory and glial markers as compared with vehicle-treated CCI animals. Conclusions Anti-inflammatory and anti-apoptotic processes might contribute to the anti-allodynic and anti-hyperalgesic effects of rosemary following nerve injury. Our findings support the ethnopharmacological use of rosemary as a potential candidate in treating neuropathic pain and different neurological disorders that associate with the activation of apoptosis and inflammatory pathways. © 2016 Elsevier Ireland Ltd
Golmohammadi R.,Sabzevar University of Medical science |
Sabaghzadeh F.,Hakim Sabzevari University |
Mojadadi M.S.,Sabzevar University of Medical science
Research in Pharmaceutical Sciences | Year: 2016
Anethum graveolens or Dill (local name: Shevid) belongs to the family of Apiaceae (Umbelliferae) and is used traditionally for the treatment of convulsion and diabetes in Iran. This study aimed to investigate the effect of hydroalcoholic extract of A. graveolens leaves on the histology of the dentate gyrus of the hippocampus in the epileptic mice kindled by Pentylenetetrazole (PTZ). In this experimental study, the epileptic BALB/c mice kindled by PTZ were randomly divided into four groups of 10 animals each. Three experimental groups received 250, 500 and 750 mg/kg/day of A. graveolens extract for 21 days. The control group received phosphate-buffered saline (PBS). After the treatment period, the mice were anesthetized, and their hippocampi were dissected for the histopathological analysis, and immunohistochemical analysis for caspase-3 activity. Histopathological examinations showed that the mean numbers of the healthy neuronal cells in the dentate gyrus of the mice received 500 mg/kg/day of A. graveolens extracts were significantly higher than those of the mice received 250 and 750 mg/kg/day of the extracts as well as the control group (P < 0.05 and P < 0.001, respectively). In addition, the results of immunohistochemical analysis revealed that in mice treated with 500 mg/kg/day of A. graveolens; the numbers of caspase-3-positive cells in the dentate gyrus were significantly lower than those of the two other test and the control groups. The findings of this study suggest that 500 mg/kg/day of the A. graveolens extract could have protective effect on the dentate gyrus of the hippocampus in the epileptic mice.
Gholami O.,Sabzevar University of Medical science |
Shamsara J.,Mashhad University of Medical Sciences
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2016
Umbelliprenin and auraptene structurally belong to the class of coumarins. Umbelliprenin can be synthesized chemically or extracted by maceration at room temperature. Various biological effects for both umbel lip renin and auraptene have been reported. One of their most important effects is cytotoxicity. This finding has increased interest in the application of umbelliprenin and auraptene as novel chemotherapeutic agents. In several studies, umbelliprenin and auraptene were successfully evaluated for anti-cancer effect. Moreover, mechanistic studies have attempted to find the mechanism of action of umbelliprenin and auraptene. In this review, we describe the cytotoxic effects of umbelliprenin and auraptene and compare them with each other. Umbelliprenin and auraptene share some cytotoxic and apoptotic induction properties but have differences in the suggested mechanisms. © 2016 The Authors. Published by Innovare Academic Sciences Pvt Ltd.
Lokk J.,Huddinge Hospital |
Delbari A.,Huddinge Hospital |
Delbari A.,Sabzevar University of Medical science
BMC Palliative Care | Year: 2012
Parkinson's disease (PD) is one of the most common neurodegenerative disorders of the elderly population. Few therapeutic options are available for patients with PD requiring palliative care. Treatment of the early stages of PD is entirely different from later stages. During the later stages, the palliative care model is introduced to provide the patient with comfort and support. Early palliative care in PD requires minimization of dyskinesias and decreasing occurrence of motor and non-motor off times in an effort to maximize independent motor function. In the later stages, the focus of treatment shifts to treating the predominant non-motor symptoms and having a more supportive and palliative nature. The purpose of this review is to provide a summary of the palliative care management issues and palliative care management options of end-stage PD patients. © 2012 Lokk and Delbari; licensee BioMed Central Ltd.
Ramshini H.,University of Tehran |
mohammad-zadeh M.,Sabzevar University of Medical science |
Ebrahim-Habibi A.,Tehran University of Medical Sciences
International Journal of Biological Macromolecules | Year: 2015
Clinical application of curcumin for Alzheimer's disease treatment is severely limited with regard to its poor bioavailability, high rate of metabolism, and instability under neutral condition. In the current study, we designed three compounds in which the diketone moiety of curcumin was replaced by cyclohexanone. In these compounds, the linker length of the molecules was optimal; and substitution of dioxolane for hydroxyl groups on compound 3 should prevent metabolic inactivation. The inhibitory effect of the compounds was investigated against hen egg white lysozyme (HEWL) fibrillation using AFM (atomic force microscope), ThT (thioflavin T) and MTT assay. We found that all three compounds were able to inhibit HEWL aggregation in a dose-dependent manner and inhibit the cytotoxic activity of aggregated HEWL. Docking results demonstrated that the compounds could bind into lysozyme and occupy the whole active site groove. In conclusion, we present chemical analogs of curcumin with various modifications in the spacer and the phenolic rings as improved inhibitors of amyloid aggregation. © 2015 Elsevier B.V.
Shomoossi N.,Sabzevar University of Medical science
Acta Facultatis Medicae Naissensis | Year: 2013
The interaction between non-English authors of medical articles and translators can be considered as a key component in perfect rendering of the content. In the era of rapid spread of research findings throughout the world, some researchers with little knowledge of English look for translation and editing services in order to share their findings in the global dialogue of science production. This paper will closely investigate the protocol analysis of an interview with a medical researcher and an intimate translator who has helped him for almost 10 years in translating his articles and research reports into English. The analysis will be classified in two major areas: language and publishing. Further details will be presented in the conference session.
Ranjbar R.,Baqiyatallah Medical Sciences University |
Khamesipour F.,Sabzevar University of Medical science |
Jonaidi-Jafari N.,Baqiyatallah Medical Sciences University |
Rahimi E.,Islamic Azad University
BMC Microbiology | Year: 2016
Background: Up to now, fecal-oral and oral-oral are the most commonly known routes for transmission of H. pylori, therefore, contaminated water can play an important role in transmission of H. pylori to humans. Genotyping using virulence markers of H. pylori is one of the best approaches to study the correlations between H. pylori isolates from different samples. The present research was carried out to study the vacA, cagA, cagE, oipA, iceA and babA2 genotyping and antimicrobial resistance properties of H. pylori isolated from the bottled mineral water samples of Iran. Results: Of 450 samples studied, 8 samples (1.77 %) were contaminated with H. pylori. Brand C of bottled mineral water had the highest prevalence of H. pylori (3.63 %). The bottled mineral water samples of July month had the highest levels of H. pylori-contamination (50 %). H. pylori strains had the highest levels of resistance against metronidazole (62.5 %), erythromycin (62.5 %), clarithromycin (62.5 %), amoxicillin (62.5 %) and trimethoprim (62.5 %). Totally, 12.5 % of strains were resistant to more than 6 antibiotics. VvacAs1a (100 %), vacAm1a (87.5 %), cagA (62.5 %), iceA1 (62.5 %), oipA (25 %), babA2 (25 %) and cagE (37.5 %) were the most commonly detected genotypes. M1as1a (62.5 %), m1as2 (37.5 %), m2s2 (37.5 %) and S1a/cagA+/IceA2/oipA-/babA2-/cagE-(50 %) were the most commonly detected combined genotypes. Conclusions: Contaminated bottled mineral water maybe the sources of virulent and resistant strains H. pylori. Careful monitoring of bottled mineral water production may reduce the risk of H. pylori transmission into the human population. © 2016 Ranjbar et al.
Foji S.,Sabzevar University of Medical science |
Tadayonfar M.A.,Sabzevar School of Medical Sciences |
Mohsenpour M.,University of Social Welfare and Rehabilitation Sciences |
Rakhshani M.H.,Sabzevar University of Medical science
Complementary Therapies in Clinical Practice | Year: 2015
One of the most frequently used methods for the diagnosis and treatment of heart diseases is cardiac catheterization which increases the anxiety level in patients before surgery. In this random clinical study, 62 people undergoing coronary angiography were randomly assigned to experimental and control groups. The necessary data were collected through a hemodynamic variable sheet, the STAI anxiety questionnaire and a pain scale. The experimental group subjects were asked to listen to a Guided Imagery CD for 18min. The mean level of anxiety for the Guided Imagery group after the intervention decreased significantly. The comparison of the means of hemodynamic parameters before and after the intervention showed a small decrease after the intervention, but this reduction was not statistically significant. Moreover, the means of pain as measured by pain scale showed that the control group had slower level of pain but, again, this difference was not statistically significant. © 2015 Elsevier Ltd.
Ghandi S.,Sabzevar University of Medical science |
Aflatoonian A.,University of Yazd |
Tabibnejad N.,University of Yazd |
Moghaddam M.H.S.,Sabzevar University of Medical science
Journal of Assisted Reproduction and Genetics | Year: 2011
Purpose: Comparing the effects of metformin or orlistat on hormone, lipid profile and ovulation status in obese women with polycystic ovary syndrome. Methods: A total of 80 women were prospectively recruited to receive either metformin (n=40) or orlistat (n=40). Weight, BMI, waist, serum LH, total serum testosterone and lipid profile were assessed at baseline and after 3 months. The subjects' ovulatory status was assessed after 3 months. Results: There was no significant difference in ovulation between the two treatment groups (30% vs 15%). Treatment with either drug showed a significant decline in body weight, BMI (Body Mass Index), and waist circumference, but the degree of decline in both groups was the same. Patients who were treated with orlistat, showed a significant reduction in total testosterone and serum lipid. Women in metformin group showed a significant reduction in serum LH. Conclusions: Both metformin and orlistat showed a similar effect on weight loss and ovulation rates. © 2011 Springer Science+Business Media, LLC.