Entity

Time filter

Source Type


Stow L.R.,Hypertension and Renal Transplantation | Stow L.R.,SABiosciences | Richards J.,Hypertension and Renal Transplantation | Richards J.,University of Florida | And 9 more authors.
Hypertension | Year: 2012

The circadian clock protein period 1 (Per1) contributes to the regulation of expression of the α subunit of the renal epithelial sodium channel at the basal level and in response to the mineralocorticoid hormone aldosterone. The goals of the present study were to define the role of Per1 in the regulation of additional renal sodium handling genes in cortical collecting duct cells and to evaluate blood pressure (BP) in mice lacking functional Per1. To determine whether Per1 regulates additional genes important in renal sodium handling, a candidate gene approach was used. Immortalized collecting duct cells were transfected with a nontarget small interfering RNA or a Per1-specific small interfering RNA. Expression of the genes for α-epithelial sodium channel and Fxyd5, a positive regulator of Na, K-ATPase activity, decreased in response to Per1 knockdown. Conversely, mRNA expression of caveolin 1, Ube2e3, and ET-1, all negative effectors of epithelial sodium channel, was induced after Per1 knockdown. These results led us to evaluate BP in Per1 KO mice. Mice lacking Per1 exhibit significantly reduced BP and elevated renal ET-1 levels compared with wild-type animals. Given the established role of renal ET-1 in epithelial sodium channel inhibition and BP control, elevated renal ET-1 is one possible explanation for the lower BP observed in Per1 KO mice. These data support a role for the circadian clock protein Per1 in the coordinate regulation of genes involved in renal sodium reabsorption. Importantly, the lower BP observed in Per1 KO mice compared with wild-type mice suggests a role for Per1 in BP control as well. © 2012 American Heart Association, Inc. Source


Li Z.,National Center for Toxicological Research (NCTR) | Branham W.S.,National Center for Toxicological Research (NCTR) | Dial S.L.,National Center for Toxicological Research (NCTR) | Wang Y.,SABiosciences | And 3 more authors.
BMC Genomics | Year: 2010

Background: Dysregulated expression of microRNAs (miRNAs) has been previously observed in human cancer tissues and shown promise in defining tumor status. However, there is little information as to if or when expression changes of miRNAs occur in normal tissues after carcinogen exposure.Results: To explore the possible time-course changes of miRNA expression induced by a carcinogen, we treated mice with one dose of 120 mg/kg N-ethyl-N-nitrosourea (ENU), a model genotoxic carcinogen, and vehicle control. The miRNA expression profiles were assessed in the mouse livers in a time-course design. miRNAs were isolated from the livers at days 1, 3, 7, 15, 30 and 120 after the treatment and their expression was determined using a miRNA PCR Array. Principal component analysis of the miRNA expression profiles showed that miRNA expression at post-treatment days (PTDs) 7 and 15 were different from those at the other time points and the control. The number of differentially expressed miRNAs (DEMs) changed over time (3, 5, 14, 32, 5 and 5 at PTDs 1, 3, 7, 15, 30 and 120, respectively). The magnitude of the expression change varied with time with the highest changes at PTDs 7 or 15 for most of the DEMs. In silico functional analysis of the DEMs at PTDs 7 and 15 indicated that the major functions of these ENU-induced DEMs were associated with DNA damage, DNA repair, apoptosis and other processes related to carcinogenesis.Conclusion: Our results showed that many miRNAs changed their expression to respond the exposure of the genotoxic carcinogen ENU and the number and magnitude of the changes were highest at PTDs 7 to 15. Thus, one to two weeks after the exposure is the best time for miRNA expression sampling. © 2010 Li et al; licensee BioMed Central Ltd. Source


Liu G.G.,SABiosciences
Advances in Experimental Medicine and Biology | Year: 2010

The use of computational applications in biological research is significantly lagging behind other scientific research areas such as physics, mathematics, and geology; more in silico tools are needed. The increasing complexity of biological data makes it more and more difficult for scientists to verify their hypotheses and results against existing discoveries. GNCPro is a free data integration and visualization tool for gaining comprehensive overviews of such complicated biological knowledge. In particular, GNCPro warehouses and encodes biological information as binary relationships. When represented graphically, these binary relationships take on the form of edges that connect the genes and proteins, which are represented by nodes. By using distinguishing features such as colors, shape, and opacity, GNCPro provides a stimulating visual experience in which the user can quickly identify groups of genes by annotations and the types of relationships involved. GNCPro integrates human gene expressions, regulations, gene product modifications, and interactions into one platform while delivering a simple and powerful user interface for systems biology study. Availability: http://GNCPro.sabiosciences.com . © 2010 Springer Science+Business Media, LLC. Source


Trademark
SABiosciences | Date: 2013-02-05

Chemical, biochemical and biotechnological products for industrial and scientific purposes, namely, diagnostic preparations except for human or veterinary medical purposes, non-medical reagents, enzymes and buffer solutions for scientific and medical research use for sample preparation, modification and manipulation of cells and for marking, separating, isolating, purifying, duplicating, sequencing and for the analysis of biopolymers, in particular nucleic acids, proteins, macromolecules and biologically active substances, in particular reagents, enzymes and buffer solutions for nucleic acid purification, enrichment, amplification, sequencing and analysis; Kits comprising chemicals for sample preparation, modification and manipulation of cells and for marking, separating, isolating, purifying, duplicating, sequencing and for the analysis of biopolymers, in particular nucleic acids, proteins, macromolecules and biologically active substances, in particular nucleic acids or biological or biochemical sample material, in particular comprising chemicals for reagents, enzymes and buffer solutions for nucleic acid purification, enrichment, amplification, sequencing and analysis. Diagnostic preparations for medical purposes; diagnostic preparations for veterinary purposes; Diagnostic preparations for humans for medical purposes; Preparations for medical and veterinary purposes, in particular diagnostics, for sample preparation, modification and manipulation of cells and for marking, separating, isolating, purifying, duplicating, sequencing and for the analysis of biopolymers, in particular nucleic acids, proteins, macromolecules and biologically active substances, in particular reagents, enzymes and buffer solutions for nucleic acid purification, enrichment, amplification, sequencing and analysis; Chemical, biochemical and biotechnological preparations, in particular reagents, enzymes and buffer solutions for sample preparation, modification and manipulation of cells and for marking, separating, isolating, purifying, duplicating, sequencing and for the analysis of biopolymers, in particular nucleic acids, proteins, macromolecules and biologically active substances, in particular reagents, enzymes and buffer solutions for nucleic acid purification, enrichment, amplification, sequencing and analysis; Kits containing preparations for medical and veterinary diagnostic purposes, in particular for sample preparation, modification and manipulation of cells and for marking, separating, isolating, purifying, duplicating, sequencing and for the analysis of biopolymers, in particular nucleic acids, proteins, macromolecules and biologically active substances, in particular reagents, enzymes and buffer solutions for nucleic acid purification, enrichment, amplification, sequencing and analysis. Scientific apparatuses, instruments and devices, as well as their parts and components, namely, PCR plates, covers for PCR plates, laboratory tubes and tissue culture plates; computer software for use in scientific and medical research; computer software in the area of analysis of nucleic acids.


Trademark
SABiosciences | Date: 2013-02-01

Chemical and biochemical reagents for non-medical purposes, and biotechnological chemicals for use in industry and science; Diagnostic preparations, other than for medical and veterinary medical purposes; Chemical reagents and chemical solvents for non-medical purposes, in particular for sample preparation, and analysis of biopolymers being nucleic acids or proteins, for modification and manipulation of cells or biopolymers being nucleic acids or proteins, and for conducting marking, separating, isolating, amplifying or purifying methods for biopolymers, in particular nucleic acids, proteins, macromolecules and biologically active substances; Kits containing chemicals used in industry and science, in particular for sample preparation and analysis of biopolymers, in particular nucleic acids or proteins, for the modification and manipulation of cells or biopolymers, in particular nucleic acids or proteins, and for the marking, separating, isolating, amplifying or purifying of biopolymers, in particular nucleic acids, proteins, macromolecules and biologically active substances, in particular nucleic acids or proteins from biological material. Medical and veterinary products, namely medical diagnostic reagents for sample preparation, analysis, modification and manipulation of cells or biopolymers, in particular nucleic acids or proteins and for the marking, separating, isolating, amplifying, or purifying of biopolymers, in particular nucleic acids, proteins, macromolecules and biologically active substances; Chemical reagents, biochemical reagents and biotechnological chemical reagents for medical or medical diagnostic purposes, in particular reagents and chemical solvents used in sample preparation, analysis, modification and manipulation of cells or biopolymers in particular nucleic acids or proteins, and for the marking, separating, isolating, amplifying or purifying of biopolymers, in particular nucleic acids, proteins, macromolecules and biologically active substances; Kits containing medical and veterinary diagnostic reagents for sample preparation, analysis, modification and manipulation of cells or biopolymers, in particular nucleic acids or proteins, and for the marking, separating, isolating, amplifying or purifying of biopolymers, in particular nucleic acids, proteins, macromolecules and biologically active substances being nucleic acids or proteins from biological material. Scientific apparatuses, instruments and devices, as well as their parts and components included in this class; Software for use in scientific and medical research; Scientific apparatuses, instruments and devices, software in the area of analysis of nucleic acids.

Discover hidden collaborations