Sabarmati Ashram Gaushala

Kheda, India

Sabarmati Ashram Gaushala

Kheda, India
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Chaudhari D.V.,Sabarmati Ashram Gaushala | Dhami A.J.,Anand Agricultural University | Hadiya K.K.,Anand Agricultural University | Patel J.A.,Anand Agricultural University
Veterinary World | Year: 2015

Aim: The aim was to compare commercially available soybean milk-based extenders, viz. Bioxcell® and Optixcell® (IMV, France) with standard Tris-citrate-fructose-egg yolk-glycerol (TFYG) extender for cryopreservation of buffalo semen. Materials and Methods: Semen was collected twice a week in artificial vagina from six sexually mature, 4-6 years old, healthy breeding bulls of Surti buffalo breed. In all 48 qualifying ejaculates (8 per bull) having initial motility >70% were split into three equal aliquots and were diluted (at 34°C keeping 100×106 sperm ml-1) in TFYG, Bioxcell and Optixcell extenders. The French mini straws filled from each aliquot were gradually cooled to 4-5°C, equilibrated at 4°C for 4 h and frozen in liquid nitrogen 2 vapor using programmable biofreezer. Just before freezing (post-equilibration) and 24 h after frozen storage, the samples were evaluated for various sperm quality parameters using standard protocols. Frozen semen straws were thawed in a water bath at 37°C for 30 s. The post-thaw incubation survival (37°C for 1 h) was assessed through motility rating at 0, 30 and 60 min of incubation. Results: The mean percentages of prefreeze sperms in TFYG, Bioxcell and Optixcell extenders in terms of progressive motility (69.48±0.37, 68.02±0.49, 70.94±0.38), viability (79.21±0.39, 77.38±0.48, 81.58±0.38), total abnormalities (7.90±0.14, 8.60±0.16, 7.08±0.15), intact acrosome (89.54± 0.18, 88.58±0.22, 90.52±0.21) and hypoosmotic swelling (HOS) reactivity (67.96±0.32, 65.65±0.42, 70.23±0.37) varied significantly (p>0.05) between extenders. Similar pattern of significant (p<0.05) variations between these extenders for post-thaw sperm progressive motility (47.71±0.79, 44.38±0.85, 49.90±0.90), viability (57.19±0.79, 53.85±0.84, 59.67±0.91), total abnormalities (12.33±0.17, 12.75±0.21, 11.27±0.18), intact acrosome (76.83±0.23, 75.90± 0.27, 78.50±0.25) and HOS reactivity (45.02±0.84, 42.31±0.82, 47.81±0.90) was also observed for TFYG, Bioxcell and Optixcell extenders. The recently launched improved soybean milk-based extender Optixcell excelled the older Bioxcell extender and even standard TFYG in respect of some of the sperm quality parameters. Conclusion: The advantages of soy lecithin-based bovine semen extenders over egg yolk regarding sanitary issues are unquestionable but still egg yolk-based semen extenders are widely used because of the cost factor and good in vivo fertility results. © The authors.


Anilkumar R.,University of Veterinary and Animal Sciences | Sundararaman M.N.,Madras Veterinary College | Patel D.V.,Sabarmati Ashram Gaushala | Iyue M.,University of Veterinary and Animal Sciences | Kasiraj R.,Sabarmati Ashram Gaushala
Buffalo Bulletin | Year: 2017

Toda buffaloes are the endangered species of the Nilgiri Hills of South India. Successful attempts have been made in cryopreservation of semen of Toda bulls. Thawed semen samples from Toda buffalo bulls were used to analyze the spermatozoal morphometry by Computer assisted semen analyzer (CASA). Head length (major axis), width (minor axis), area, perimeter, elongation and tail length were the morphometrical characteristics measured. The mean (±SE) length, width, area, perimeter, elongation percentage and tail length were 7.38±0.02 µm, 4.68±0.02 µm, 28.53±0.17 µm2, 20.32±0.19 µm, 62.95±0.22% and 52.93±0.41 µm respectively. Overall spermatozoal abnormality was 30.75% and coiled tail was the most common (50.49%) abnormality. The spermatozoal morphometry reported in this study may form a preliminary data in Toda buffalo semen analysis and helps for comparison with other breeds and can complement sperm motility assessment. © 2017, Kasetsart University. All rights reserved.


Anil Kumar R.,University of Veterinary and Animal Sciences | Iyue M.,University of Veterinary and Animal Sciences | Patel D.V.,Sabarmati Ashram Gaushala | Kasiraj R.,Sabarmati Ashram Gaushala
Buffalo Bulletin | Year: 2017

As a breed conservation measure in Toda buffaloes, a unique, ferocious, semi wild breed of Nilgiris district of Tamilnadu, whose population is dwindling over the years, an attempt has been made towards the collection and cryopreservation of embryos. Toda buffalo cows (10) and Toda bulls (3) used for the study were representative samples of the breed sourced from Toda hamlets in the breeding tract and maintained under organized farm conditions. The response for superovulation was 93.33% (28/30) and 82.14% (23/28) of the animals were flushed. A total of 39 embryos were recovered, of this 26 viable embryos were cryopreserved. The viable embryo recovery rate per animal flushed was 1.13. buffaloes administered with 400 mg of FSH responded higher than those administered with 600 mg. Buffaloes with bigger superovulatory corpus luteum (SOV CL) had significantly higher ovulation rate (3.50 ± 0.26) and viable embryo recovery (1.46 ± 0.49) than animals with medium or small SOV CL. The viable embryo recovery rate was higher on 10th day SOV (1.35 ± 0.36) than on 9th day (0.75 ± 0.75) of starting of FSH treatment. Donors in standing estrum yielded a significantly higher number of total embryos (2.00 ± 0.41 vs 0.60 ± 0.30) and viable embryos (1.33 ± 0.36 vs 0.40 ± 0.40) than the donors with non standing estrum. © 2017, Kasetsart University. All rights reserved.


Anil Kumar R.,University of Veterinary and Animal Sciences | Sundararaman M.N.,Farmers Training Center | Patel D.V.,Sabarmati Ashram Gaushala | Iyue M.,University of Veterinary and Animal Sciences | Kasiraj R.,Sabarmati Ashram Gaushala
Buffalo Bulletin | Year: 2012

Conservation of endangered Toda buffaloes of the Nilagiri hills of South India in the form of cryopreservation of semen has been attempted. Toda bulls were reared from calfhood at this Research Station. Semen was collected from the bulls using an artificial vagina, evaluated and cryopreserved. The mean ejaculate volume of semen was 2.20 ± 0.25 ml and concentration was 1267.10 ± 107.78 million per ml. The pre-freeze motility and post thaw motility were 74.16 ± 3.60 and 43.14 ± 2.96 percent, respectively. The motility characteristics of frozen semen were assessed by computer assisted semen analyzer (CASA). Average sperm motility of frozen thawed semen was 54.50± 9.72 percent with 28.00± 7.20 percent of sperm progressively motile. The means (± SE) for path velocity (VAP), progressive velocity (VSL), track speed (VCL), lateral amplitude (ALH), beat cross frequency (BCF) were 88.79± 8.23 μm/s, 74.19± 6.21 μm/s, 137.99± 14.90 μm/s, 6.78± 0.49 μm and 16.38± 2.09 Hz respectively. The percentage of straightness (STR) and linearity (LIN) were 83.88± 1.57 and 57.50± 2.50, respectively. Semen samples with high post-thaw motility had significantly higher percentage of sperm motility (SM) and progressive motility (PSM). The samples with high PSM had higher path velocity, progressive velocity and track speed. Positive correlation was observed between VAP, VSL, VCL and ALH. Similarly, there was a high positive correlation between VSL and VCL. The mean linearity in Toda buffalo bull semen was above the acceptable threshold level of 50 percent and was of acceptable quality comparable to other buffaloes. The Toda buffalo bulls can be reared in a farm environment if they are trained from calfhood. By using a female Toda buffalo in estrum as a teaser, semen can be collected from Toda bulls in an artificial vagina. Cryopreservation can be successfully employed for conservation of Toda buffalo germplasm.


Patel D.V.,Sabarmati Ashram Gaushala | Singh S.P.,Sabarmati Ashram Gaushala | Shukla H.R.,Sabarmati Ashram Gaushala | Devanand C.P.,Sabarmati Ashram Gaushala | Kasiraj R.,Sabarmati Ashram Gaushala
Buffalo Bulletin | Year: 2010

The superovulatory responses using either 400 mg or 600 mg of FSH (Folltropin-V) in eight Pandharpuri buffaloes used repeatedly were studied. All buffaloes except one responded (minimum 2 CL/follicle) for superovulation in all seasons. Overall response (mean±SE) to superovulation was 61 ovulations (3.39±0.46), 21 follicles/cysts (1.40±0.41), 33 total embryos (1.94±0.39) and 24 viable embryo recoveries (1.41±0.32). The response was non-significantly higher in buffaloes injected with 400 mg (4.13± 0.52-total ovulations) than 600 mg (2.80±0.64). Use of different bull semen had no signifi cant difference in viable embryo recovery.

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