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Anil Kumar R.,University of Veterinary and Animal Sciences | Sundararaman M.N.,Farmers Training Center | Patel D.V.,Sabarmati Ashram Gaushala | Iyue M.,University of Veterinary and Animal Sciences | Kasiraj R.,Sabarmati Ashram Gaushala
Buffalo Bulletin

Conservation of endangered Toda buffaloes of the Nilagiri hills of South India in the form of cryopreservation of semen has been attempted. Toda bulls were reared from calfhood at this Research Station. Semen was collected from the bulls using an artificial vagina, evaluated and cryopreserved. The mean ejaculate volume of semen was 2.20 ± 0.25 ml and concentration was 1267.10 ± 107.78 million per ml. The pre-freeze motility and post thaw motility were 74.16 ± 3.60 and 43.14 ± 2.96 percent, respectively. The motility characteristics of frozen semen were assessed by computer assisted semen analyzer (CASA). Average sperm motility of frozen thawed semen was 54.50± 9.72 percent with 28.00± 7.20 percent of sperm progressively motile. The means (± SE) for path velocity (VAP), progressive velocity (VSL), track speed (VCL), lateral amplitude (ALH), beat cross frequency (BCF) were 88.79± 8.23 μm/s, 74.19± 6.21 μm/s, 137.99± 14.90 μm/s, 6.78± 0.49 μm and 16.38± 2.09 Hz respectively. The percentage of straightness (STR) and linearity (LIN) were 83.88± 1.57 and 57.50± 2.50, respectively. Semen samples with high post-thaw motility had significantly higher percentage of sperm motility (SM) and progressive motility (PSM). The samples with high PSM had higher path velocity, progressive velocity and track speed. Positive correlation was observed between VAP, VSL, VCL and ALH. Similarly, there was a high positive correlation between VSL and VCL. The mean linearity in Toda buffalo bull semen was above the acceptable threshold level of 50 percent and was of acceptable quality comparable to other buffaloes. The Toda buffalo bulls can be reared in a farm environment if they are trained from calfhood. By using a female Toda buffalo in estrum as a teaser, semen can be collected from Toda bulls in an artificial vagina. Cryopreservation can be successfully employed for conservation of Toda buffalo germplasm. Source

Patel D.V.,Sabarmati Ashram Gaushala | Singh S.P.,Sabarmati Ashram Gaushala | Shukla H.R.,Sabarmati Ashram Gaushala | Devanand C.P.,Sabarmati Ashram Gaushala | Kasiraj R.,Sabarmati Ashram Gaushala
Buffalo Bulletin

The superovulatory responses using either 400 mg or 600 mg of FSH (Folltropin-V) in eight Pandharpuri buffaloes used repeatedly were studied. All buffaloes except one responded (minimum 2 CL/follicle) for superovulation in all seasons. Overall response (mean±SE) to superovulation was 61 ovulations (3.39±0.46), 21 follicles/cysts (1.40±0.41), 33 total embryos (1.94±0.39) and 24 viable embryo recoveries (1.41±0.32). The response was non-significantly higher in buffaloes injected with 400 mg (4.13± 0.52-total ovulations) than 600 mg (2.80±0.64). Use of different bull semen had no signifi cant difference in viable embryo recovery. Source

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