Dental College and Research Center

Mathura, India

Dental College and Research Center

Mathura, India
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Saxena P.,University of Delhi | Pant V.A.,Dental College and Research Center | Wadhwani K.K.,University of Delhi | Kashyap M.P.,Indian Institute of Toxicological Research | And 2 more authors.
Dental Traumatology | Year: 2011

In vitro experiments were carried out to evaluate the potential of propolis, a natural resin known for its wide therapeutic window, as storage medium to preserve the viability of cultured human periodontal ligament (PDL) cells. Materials and Methods: Primary cultures of human PDL cells were subjected to either independent exposure of propolis (2.5%, 5.0%, 10.0%, and 20.0%), Hank's balanced salt solution (HBSS), milk (0.5%), artificial saliva, Dulbecco's modified Eagle's medium (DMEM) or combination of propolis 10%+DMEM, propolis 20%+DMEM for 30min to 24h at 37°C. Cell viability was assessed using standard endpoints i.e., tetrazolium bromide salt (MTT), neutral red uptake, and trypan blue dye exclusion assay. Results: In general, combinations of propolis 10%+DMEM, propolis 20%+DMEM, and DMEM alone were found to be better than other media used in this study. The difference in the potentials of these media to maintain the cell viability reached to the statistically significant levels by 24h, when compared with other media used viz., propolis 2.5% (P<0.01), propolis 5.0% (P<0.05), propolis 10.0% (P<0.05), propolis 20.0% (P<0.001), HBSS (P<0.001), and milk (P<0.01). Trypan blue dye exclusion assay could be recorded the most sensitive among all the assays selected to study the cell viability of PDL cells. Conclusions: Study indicates that combinations of propolis 10%+DMEM, propolis 20%+DMEM, and DMEM alone are equally good as storage media of choice to keep PDL cells viable during extra-alveolar period up to 24h. Other more readily available medium such as milk may serve as appropriate alternative storage medium for shorter time periods i.e., up to 12h. © 2011 John Wiley & Sons A/S.

Pajnigara N.G.,Dental College and Research Center | Kolte A.P.,Dental College and Research Center | Kolte R.A.,Dental College and Research Center
International Journal of Periodontics and Restorative Dentistry | Year: 2017

Volumetric changes were evaluated to assess the regenerative efficacy of demineralized freeze-dried bone allograft (DFDBA) with and without amnion membrane (AM) in Grade II furcation defects both clinically and radiographically using cone beam computed tomography (CBCT). Sites were randomly assigned to Group I (DFDBA) and Group II (DFDBA + AM). Clinical parameters such as probing pocket depth, clinical attachment level, gingival recession, and horizontal probing depth were assessed at baseline, 3 months, and 6 months. Radiographic dimensions of the defect height, width, depth, and volume were assessed at baseline and 6 months postoperatively. DFDBA used with AM resulted in significant improvement in clinical and radiographic parameters when compared with DFDBA alone. The mean reductions in radiographic volume of the defect were 11.15 ± 6.39 mL for Group I and 17.02 ± 10.86 mL for Group II. Greater reduction in volume indicated a greater amount of healed regenerated tissue in Group II patients. © 2017 by Quintessence Publishing Co Inc.

Shenoi R.,Dental College and Research Center | Devrukhkar V.,Dental College and Research Center | Chaudhuri,Cancer Institute and Research Center | Sharma B.K.,Cancer Institute and Research Center | And 2 more authors.
Indian Journal of Cancer | Year: 2012

Background: Oral cancers are one of the ten leading cancers in the world. However, in India, it is one of the most common cancer and constitutes a major public health problem. Aim: The purpose of this study was to evaluate, retrospectively, the epidemiologic profile of patients with oral squamous cell carcinoma (OSCC). Materials and Methods: OSCC cases were retrospectively analyzed from January 2008 to September 2010 for age, gender, occupation, duration of the symptoms, habits (tobacco and alcohol consumption), site of primary tumor, and TNM staging, and the findings were formulated to chart the trends in central India population. Results: Male to female ratio was 4.18:1. Mean age was 49.73 years. The most common site of presentation of tumor was in mandibular alveolus region. Tobacco chewing was the major cause for the development of OSCC. Maximum number of patients, i.e., 201 (68.14%) were presented within 6 months of onset of symptoms. Majority of patients were presented in Stage III (82.37%). Correlation between the two variables, i.e., site to habits, staging to site involved, staging to duration of the disease, staging to habits, and staging to age of the patient, were found to be statistically nonsignificant (P>0.05). Conclusions: The aim of the study was the demographic description of oral squamous cell carcinoma. Most of the cases report at advanced stages of the disease which often leads to delay in the management coupled with the fact that health care centers are burdened with long waiting lists. Strategies to overcome the present situation must be undertaken by oral health programs for the early diagnosis and prevention and management and follow up of oral cancer.

Bodhade A.S.,Dental College and Research Center
Journal of oral science | Year: 2011

Human Immunodeficiency Virus (HIV)-related oral lesions can be used as markers of the immune status. The present cross-sectional study was conducted to identify the oral manifestations in HIV-infected individuals and their association with reduced Cluster of Differentiation 4 (CD4) count. The study population included known HIV-positive patients. A detailed case history of 399 HIV-positive patients was obtained and general examination was carried out. Diagnosis of oral lesions was done based on presumptive criteria of EEC Clearinghouse, 1993. The CD4 count was determined in 369 patients and correlated with oral manifestations. The prevalence of oral lesions was found to be 76.70% (n = 306). Oral candidiasis (157 (39.3%)) was the most common oral lesion associated with HIV infection. Amongst various forms of oral candidiasis, erythematous candidiasis (122 (39.3%)) outnumbered the other forms. The mean CD4 count of patients with oral lesions (207 cells/mm(3)) was less than in patients without oral lesions (291 cells/mm(3)) (P = 0.002). Oral candidiasis was found to be significantly correlated to a reduced CD4 cell count below 200 cells/mm(3) (P = 0.000; Odds ratio = 3.1; 95% Confidence interval 1.9-4.9) with good sensitivity, best specificity and positive predictive value. Oral manifestations may be used as an alternative to CD4 count at field-based settings to diagnose the immune compromised status of HIV-infected individuals.

Ninawe N.,Dental College and Research Center
BMJ case reports | Year: 2013

Crown fracture of maxillary anterior teeth is relatively common among children and teenagers. Aesthetic rehabilitation of crown fractures of the maxillary anterior is one of the greatest challenges to the dentist. Reattachment of a fractured fragment to the remaining tooth can provide better and long-lasting aesthetics, improved function, a positive psychological response and is a faster and less-complicated procedure. This article presents a case of reattachment of anterior tooth with a coronal fracture involving enamel, dentin and pulp with a 1.5-year follow-up.

Chaudhari P.,Dental College and Research Center
BMJ case reports | Year: 2013

A 4-year-old boy reported food lodgement and pain in the lower left back tooth region. On examination, a deeply carious tooth with food lodgement was seen. On oral examination, numbers of teeth were found to be carious and required restorations and endodontic treatments according to radiographic evaluation. Radiograph of mandibular left first deciduous molar revealed an unusual morphology of root. It was single-rooted and presented with Vertucci's class I canal. The tooth was treated by pulpectomy followed by a stainless steel crown. All other carious teeth were treated as planned.

Shilpashree H.S.,Dental College and Research Center | Sarapur S.,Dental College and Research Center
Journal of Natural Science, Biology and Medicine | Year: 2012

Objectives: The aim of the present study was to analyze the influence of smoking on the salivary immunoglobulin response in smokers and to evaluate the salivary immunoglobulin A in patients with recurrent aphthous ulcers. Materials and Methods: The study included total of 80 subjects, of whom 40 were having history of chronic smoking habit, 20 were clinically diagnosed cases of recurrent aphthous ulcer and 20 were in the control group. Sample of unstimulated saliva was collected, centrifuged and analyzed for the level of salivary immunoglobulin A with turbidimetric immunoassay. For all the tests, a P- value of < 0.05 was considered for statistical significance. Results: The mean salivary immunoglobulin A level in control group was 0.20 Grams/litre and in smokers the mean salivary immunoglobulin A level was 0.13 Grams / Litre. In patients with recurrent aphthous ulcers mean salivary immunoglobulin A level was 0.31 Grams / Litre. The mean salivary immunoglobulin A levels showed a decreasing trend from controls to smokers. These results were highly significant for values between control groups to smokers. Conclusion: The mean salivary immunoglobulin A levels demonstrated a progressive decrease from controls to smokers. This investigative procedure although non-specific, can be used as a diagnostic marker in smokers and patients with recurrent aphthous ulcers.

Shilpashree H.S.,Dental College and Research Center | Sarapur S.,Dental College and Research Center
Journal of Pharmacology and Pharmacotherapeutics | Year: 2012

Ciprofloxacin is one of the most commonly used antibacterial agents with relatively few side effects. Serious adverse reactions reported with ciprofloxacin are rare with an incidence of 0.6%. One of the side effects of ciprofloxacin is erythema multiforme (EM). EM is an acute, self -limiting mucocutaneous hypersensitivity syndrome. It exhibits a diverse etiology, often recurs, has unusual clinical features and is of uncertain etiopathogenesis. It usually exhibits a distinctive skin or mucosal lesions that are characterized by combination of bullae, papules, macules or ulcers. It is most probably an immunologically mediated process. With the use of ciprofloxacin becoming more and more widespread, fatal complications of ciprofloxacin should be borne in mind. In this article we present a case of ciprofloxacin induced erythema multiforme in 40 year old woman.

Kolte A.P.,Dental College and Research Center | Kolte R.A.,Dental College and Research Center | Mishra P.R.,Dental College and Research Center
Journal of Periodontology | Year: 2014

Background: Restoring papillae in the gingival embrasures of the esthetic zone is a key consideration in periodontal, restorative, and orthodontic treatment. This study analyzes the existence of interdental papillae with regard to the vertical dimensions between the contact point and the alveolar crest and horizontal dimensions between adjacentroots.Methods: A total of 259 papillae, in 60 patients, were examined.All patients were indicated for full-thickness periodontal flap surgery. A mucoperiosteal flap was raised and, after thorough debridement, horizontal and vertical dimensions between adjacent roots were recorded. The incidence of papillae was analyzed using x2 for linear trend analysis. Results: Interdental papillae were present in 85.7% of the cases when the vertical dimensions were 4 mm (P <0.05).Also, papillae were present in 78.5% of cases when the horizontal dimensions were between 0.5 and 1 mm (P <0.05). Conclusion: The vertical and horizontal dimensions of interproximal areas had a substantial, independent, and combined effect on the existence of interproximal papillae. J Periodontol 2014;85:795-801.

Shilpashree H.S.,Dental College and Research Center
The New York state dental journal | Year: 2013

Gene therapy is an emerging field of biomedicine that has commanded considerable scientific and popular attention. Genes are specific sequences of bases that encode instructions to make proteins. When genes are altered so that encoded proteins are unable to carry out their normal functions, genetic disorders can result. Gene therapy essentially consists of introducing specific genetic material into target cells to compensate for abnormal genes or to make a beneficial protein without producing toxic effects on surrounding tissue. Transferred genes can be used for either reparative or pharmacological purposes. Applications of gene therapy to dental and oral problems illustrate the potential impact of this technology on dentistry. This review provides an update on transfer techniques and clinical implications of gene therapy in dentistry.

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