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Severin E.S.,Russian Research Center for Molecular Diagnostics and Therapy
Russian Chemical Reviews | Year: 2015

Basic approaches to the design of targeted drugs for the treatment of human malignant tumours have been considered. The stages of the development of these approaches have been described in detail and theoretically substantiated, and basic experimental results have been reported. Considerable attention is paid to the general characteristic of nanopharmacological drugs and to the description of mechanisms of cellular interactions with nanodrugs. The potentialities and limitations of application of nanodrugs for cancer therapy and treatment of other diseases have been considered. The use of nanodrugs conjugated with vector molecules seems to be the most promising trend of targeted therapy of malignant tumours. © 2015 Russian Academy of Sciences and Turpion Ltd Source


Urusov A.E.,Russian Academy of Sciences | Kostenko S.N.,Russian Academy of Sciences | Sveshnikov P.G.,Russian Research Center for Molecular Diagnostics and Therapy | Zherdev A.V.,Russian Academy of Sciences | Dzantiev B.B.,Russian Academy of Sciences
Sensors and Actuators, B: Chemical | Year: 2011

An immunoanalytical system was developed for the determination of ochratoxin A with the use of a surface plasmon resonance (SPR) sensor amplified by the anti-species antibody-colloidal gold particle (CGP) conjugate. The use of the binding of immune complexes to the CGP-anti-species antibody conjugate leads to the SPR signal amplification by a factor of more than 10 and results in the 60 pg/mL limit of detection of ochratoxin A with an assay time of 30 min. These characteristics are superior to those obtained both in the conventional enzyme immunoassay with the use of the same reagents and the SPR assay with unmodified antibodies and specific antibodies conjugated to colloidal gold. © 2011 Elsevier B.V. Source


Urusov A.E.,Russian Academy of Sciences | Kostenko S.N.,Russian Academy of Sciences | Sveshnikov P.G.,Russian Research Center for Molecular Diagnostics and Therapy | Zherdev A.V.,Russian Academy of Sciences | Dzantiev B.B.,Russian Academy of Sciences
Journal of Analytical Chemistry | Year: 2011

The detection of mycotoxins-toxic contaminants of fungal origin-is an important problem in the food and feed quality control. An immunochromatographic system was developed for the detection of ochratoxin A (OTA), which is one of the priority contaminants in grain. Monoclonal antibodies against OTA and their conjugates with colloidal gold nanoparticles were prepared. The detection is based on the competition of OTA in a sample and an OTA-protein conjugate immobilized on a test strip for the binding to anti-bodies on the colloidal particle surface. The method was tested in the analysis of plant extracts (maize and barley extracts). It was shown that OTA can be detected in a medium with a high content of an organic solvent (up to 35% of methanol). The disappearance of the line in the test zone is visually detected at OTA concentrations starting from 50 ng/mL. In the case of the video-digital detection of changes in the color intensity of the test zone, the limit of detection of OTA is 5 ng/mL. The duration of the assay is 10 min. © 2011 Pleiades Publishing, Ltd. Source


Byzova N.A.,Russian Academy of Sciences | Zvereva E.A.,Russian Academy of Sciences | Zherdev A.V.,Russian Academy of Sciences | Eremin S.A.,Moscow State University | And 2 more authors.
Analytica Chimica Acta | Year: 2011

A rapid pretreatment-free immunochromatographic assay was developed for the control of the streptomycin (STR) content in milk and dairy products. The assay is based on the competition between an immobilized STR-protein conjugate and STR in a sample to be tested for the binding to monoclonal anti-STR antibodies conjugated to colloidal gold during the flow of the sample along a membrane strip with immobilized reactants. It is possible to improve the cut-off level of positive and negative samples distinguished by a change in the molar STR to protein ratio in the immobilized conjugate. The cut-off level (500ngmL -1) thus achieved corresponds to the stated MRL of STR in milk and dairy products. For STR concentrations in the range of 16-250ngmL -1 its content can be quantitatively measured based on the degree of binding of a colloidal gold label in the test strip zone with the immobilized STR-protein conjugate. The duration of the assay is 10min. The selected sizes of membrane pores and colloidal gold particles allow the assay to be carried out at room temperature without additional reactants and pretreatment. The applicability of the assay for milk, whole milk, sour clotted milk, and kefir with different fat content (from 0.5% to 6%) was confirmed. The results of quantitative immunochromatographic assay show good correlation with traditional ELISA (r was equal to 0.935 and 0.940 for the series tested). © 2011 Elsevier B.V. Source


Hendrickson O.D.,RAS A.N. Bach Institute of Biochemistry | Smirnova N.I.,RAS A.N. Bach Institute of Biochemistry | Zherdev A.V.,RAS A.N. Bach Institute of Biochemistry | Sveshnikov P.G.,Russian Research Center for Molecular Diagnostics and Therapy | Dzantiev B.B.,RAS A.N. Bach Institute of Biochemistry
Microchimica Acta | Year: 2015

The article describes a highly sensitive single-step microplate enzyme immunoassay of the ELISA type for fullerene C60 and its derivatives. Monoclonal anti-fullerene antibodies and a conjugate between fullerene and horseradish peroxidase were used as specific reagents. A direct competitive ELISA was carried out that was based on antibodies immobilized in the well of a microtiter plate, a peroxidase-labeled antigen, and detection via the dye formed from 3,3′,5,5′-tetramethylbenzidine and hydrogen peroxide. Both pristine fullerene C60 and its water-soluble forms can be determined. The detection limits are 1.5 ng∙mL−1 for fullerene C60, and between 0.1 and 1.3 ng∙mL−1 for its derivatives. This ELISA format allows for almost two-fold reduction of the time needed for the assay in comparison to indirect scheme with labeled antibodies. [Figure not available: see fulltext.] © 2015 Springer-Verlag Wien Source

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