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Grigor'ev Y.G.,Russian Federal Medical Biological Agency | Sidorenko A.V.,Belarusian State University
Biophysics | Year: 2011

Development of the convulsive syndrome occurs during exposure to electromagnetic fields (EMFs) during physiotherapy and under conditions of professional work. Some researchers believe that EMFs may have medical effects during the treatment of the epilepsy syndrome. Some publications have noted the development of epileptic convulsions in experiments using EMF exposures at various frequencies. Four conditions that may promote the development of convulsions upon EMF exposure are considered. © 2011 Pleiades Publishing, Ltd.


PubMed | Institute of Experimental Medicine, Russian Federal Medical Biological Agency and Belarusian State University
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

Myeloperoxidase, heme enzyme of azurophilic granules in neutrophils, is released into the extracellular space in the inflammation foci. In neutrophils, it stimulates a dose-dependent release of lactoferrin (a protein of specific granules), lysozyme (a protein of specific and azurophilic granules), and elastase (a protein of azurophilic granules). 4-Aminobenzoic acid hydrazide, a potent inhibitor of peroxidase activity of myeloperoxidase, produced no effect on neutrophil degranulation. Using signal transduction inhibitors (genistein, methoxyverapamil, wortmannin, and NiCl2), we demonstrated that myeloperoxidase-induced degranulation of neutrophils resulted from enzyme interaction with the plasma membrane and depends on activation of tyrosine kinases, phosphatidylinositol 3-kinases (PI3K), and calcium signaling. Myeloperoxidase modified by oxidative/halogenation stress (chlorinated and monomeric forms of the enzyme) lost the potency to activate neutrophil degranulation.


Limantseva O.A.,RAS Vernadsky Institute of Geochemistry and Analytical Chemistry | Potapov E.G.,Russian Federal Medical Biological Agency | Danilov S.P.,Russian Federal Medical Biological Agency | Cherkasova E.V.,RAS Vernadsky Institute of Geochemistry and Analytical Chemistry
Geochemistry International | Year: 2016

Computer simulations of carbon dioxide leaching of Aptian–Albian sandstone at the Nagutskoe groundwater field, Caucasian Mineral Waters, are compared with laboratory experimental data obtained using a high-pressure autoclave under parameters close to conditions under which mineral waters are formed at the Nagutskoe and Essentuki fields (temperatures 20–25 and 65–70°C, carbon dioxide pressure up to 4.04 MPa). The solvents were distilled water and naturally occurring groundwaters from the Caucasian Mineral Waters (CMW) area, individual experimental runs lasted for 2 h, the starting material (rock) was crushed to 0.25 mm, and the gas phase was carbon dioxide. In most of the experiments, the solid: liquid phase (R/W) ratio was 1: 5 and was varied from 1: 10 to 1: 100 in other experiments. Our simulation results indicate that multiple-cycle (10 cycles) leaching leads to an increase in mineralization from 1.3 g/L to 4 g/L and transformation of the geochemical type of the waters from the hydrocarbonate calcic–sodic one (leaching cycle 1) to chloride–hydrocarbonate sodic (cycles 5 and later). The mineralization increased mostly because the and Na+ ions are transferred into solution at an insignificant increase in the Cl concentration and a practically unchanging concentrations of the sulfate, calcium, and magnesium ions. With regard for the averaged mineralogical composition of the sandstone (quartz, feldspars, mica, glauconite, magnetite, ilmenite, garnet, rutile, zircon, and tourmaline) used in our thermodynamic simulations, we arrived at the conclusion that the chemical compositions of the waters, including their minor-element compositions, are controlled by (i) the composition of the cement (clay, calcareous, siliceous, limonitic, chloritic, zeolitic, phosphate, sulfate, or mixed) of the rocks, (ii) weight percentages of minerals containing certain elements, and (iii) temperature, at a given composition of the gas phase of the simulated system (silty sandstone–rainwater–CO2 gas phase). © 2016, Pleiades Publishing, Ltd.


Kurdyukov I.,Russian Federal Medical Biological Agency | Rodionov G.,Academy Lebedev St | Radilov A.,Russian Federal Medical Biological Agency | Babakov V.,Russian Federal Medical Biological Agency
Analytical and Bioanalytical Chemistry | Year: 2014

Paraoxonase-1 (PON1) and butyrylcholinesterase (BCHE) are natural bioscavengers of organophosphate acetylcholinesterase inhibitors in the human body, which can determine individual sensitivity to organophosphate toxicity. Interindividual differences in activity of PON1 (catalytic bioscavenger) and substrate specificity are strongly associated with the substitution of two amino acids: Leu/Met (L/M) at position 55 (rs854560) and Gln/Arg (Q/R) at position 192 (rs662). In the case of BCHE (stoichiometric bioscavenger) substitution, Ala/Thr (A/T) at position 539 produces the so-called "K-variant" of the enzyme (rs1803274). Threonine allele is often co-inherited with an atypical BCHE allele (rs1799807). The atypical variant of BCHE displays a lower affinity for cholinesterase inhibitors. Genotyping rs662 and rs1803274 single-nucleotide polymorphisms (SNP) by high-resolution melting (HRM) is facilitated by the nucleotide substitution A>G (G>A), which resulted in a changed number of hydrogen bonds in the PCR product and, consequently, shifted T m. In the case of rs854560, genotyping is complicated by the nucleotide substitution T>A, which has no significant effect on the T m of the PCR product. An addition of a small quantity of LL homozygote DNA into the reaction mixture before PCR discriminates the three genotypes by the melt curves due to different amounts of heteroduplexes formed in the LM and MM samples. HRM analysis can be applied for genotyping human rs854560, rs662, and rs1803274 SNPs. © 2014 Springer-Verlag Berlin Heidelberg.


Smagin N.V.,Russian Academy of Sciences | Krutyansky L.M.,Russian Academy of Sciences | Zelenova Z.V.,Russian Federal Medical Biological Agency | Brysev A.P.,Russian Academy of Sciences
Acoustical Physics | Year: 2014

Acoustic absorption has been measured in a series of biological tissue specimens - porcine muscle, renal and fat tissues - by the standard insert-substitution method, as well as by ultrasonic phase conjugation. Comparison of the experimental results and revealed differences confirm the promise of using phase conjugate waves to measure acoustic losses in biological objects. It is demonstrated that in inhomogeneous tissues, the phase conjugation method makes it possible to obtain a more reliable estimate of dissipative losses. © 2014 Pleiades Publishing, Ltd.


Savel'Eva E.E.,Bashkir State Medical University | Kuznetsov A.O.,Russian Federal Medical Biological Agency
Voprosy Prakticheskoi Pediatrii | Year: 2015

The objective: Improvement of the quality of electroacoustic correction of hearing in infants suffering sensorineural hearing loss. Patients and methods: A group of 36 children aged 1 month to 5 years with bilateral sensorineural hearing loss of varied severity was clinically examined and observed in 2008-2011. The severity of hearing loss in children of the group was determined according to the findings of a complex audiological examination with the use of subjective and objective methods. Results: Recording of steady-state auditory evoked potentials to modulated tones (Auditory Steady-State Response (ASSR) testing) successfully complements the examination of functions of the auditory analyzer in children, permitting to use even very loud stimuli. The clinical study showed that the specificity of the ASSR test is 96.7%, and sensitivity - 97.2%. On the other hand, thresholds of hearing have fluctuations at various frequencies in 18% of cases, which necessitates their rechecking and comparison with the results of other tests. The dynamic follow-up showed that if a hearing disorder was diagnosed before 6-12 months of age and early adequate rehabilitation begins, a child has a good level of speech development by 3 years of age. And vice versa, children whose hearing disorder was diagnosed at 12-24 months had delayed speech development by 3 years of age as compared with same-age peers. Conclusion: The use of ASSR testing successfully complements examination of the functions of the auditory analyzer in small children. In our opinion, frequency thresholds of the ASSR test should not be used for adjustment of hearing AIDS because of their variability and difference from data of tone audiometry. The results of a child's speech development depend on the correctness of the determined frequencies of auditory thresholds, early diagnosis of hearing loss, adequate correction of hearing, and paedagogic work.


Merkulov O.A.,Moscow State University | Garashchenko T.I.,Russian Federal Medical Biological Agency
Voprosy Prakticheskoi Pediatrii | Year: 2013

The objective. To determine the possibility and effectiveness of a multidisciplinary approach to surgical treatment of neoplasms of the anterior and middle cranial fossae in children through endoscopic endonasal approach with the use of a computerassisted navigation system. Patients and methods. The study included 54 patients aged from 1 month to 18 years (mean age - 11 ± 6.62 years) with neoplasms of the anterior and middle cranial fossae. All the patients were operated for the first time through endoscopic endonasal approach: an endoscope was introduced to tumours of the anterior cranial fossa and the olfactory crest through a combined transnasal/transethmoidal «corridor»; to tumours of the Turkish saddle and the suprasellar cistern, and also to the upper third of the clivus - through transsphenoidal/transethmoidal «corridor». In planning operations, additional orientation was provided by the computer-assisted navigation system Vector Vision Compactsystem (BrainLab, USA). Results. The study showed that constructive specificities of endoscopes and improvement of orientation in the most important anatomic structures due to the use of navigation equipment ensured precise control over surgical instrument in narrow anatomic spaces and permitted to more carefully act on a pathological site. The average time of operative intervention was 248.6 ± 12.8 min. Complications developed in 9 (16.7%) patients. Intraoperative complications were registered in 3 of them (liquorrhea - in 2 and bleeding from the cavernous sinus - in 1 child). The post-operative period was complicated in 1 child by development of meningitis, and in 4 - by residual phenomena. Analysis of short-term results of the operative interventions showed regress of hormonal disorders in 50 (92.6%) patients with similar hormonal imbalance, and in 52 (96.2%) - decrease of ophthalmoneurological deficiency. Relapse of tumour occurred in 4 (7.4%) children. There were no lethal outcomes. Conclusion. As has been found, endoscopic endonasal surgery on the skull base is highly effective and safe for treatment of children with neoplasms of the anterior and middle cranial fossae. Modern computer-assisted navigation equipment permits to choose a more conservative variant of operative intervention.


Garashchenko T.I.,Russian Federal Medical Biological Agency | Boykova N.E.,Russian Federal Medical Biological Agency
Voprosy Prakticheskoi Pediatrii | Year: 2013

Tonsillites and tonsillopharyngites are a polyetiologic group of diseases of the upper respiratory tract characterized by similar clinical signs. The problem of administering startup therapy in tonsillopharyngitis is conditioned by the necessity of choosing between the use of systemic and local antibacterial therapy, which should be based on the etiology of the process. Since in 70% of cases, the causative agents of tonsillites and tonsillopharyngites are viruses, in case of ruling out the group A streptococcal etiology of disease, treatment might be restricted to administration of topical antiseptics. The article presents data about the criteria of administering various groups of antibacterial medications, and also about modern possibilities of symptomatic therapy in this group of diseases.


Manovitskaya A.V.,Russian Federal Medical Biological Agency
Voprosy Pitaniia | Year: 2011

Study on the influence of taurines (Dibicor) on metabolic indicators in patients with of metabolic syndrome showed improvement in carbohydrate metabolism and lipid profile in patients with type 2 pancreatic diabetes.


Savel'Eva E.I.,Russian Federal Medical Biological Agency | Gustyleva L.K.,Russian Federal Medical Biological Agency
Russian Journal of General Chemistry | Year: 2014

The data on properties and existence forms of Russian poisonous substance VX (RVX) in environmental objects are presented. Hydrolysis of RVX with excessive amount of water in acidic medium as well as autocatalytic hydrolysis with equimolar amount of water were investigated. It was shown that in the first case destruction of RVX proceeded slower with more diverse composition of the products. © 2014 Pleiades Publishing, Ltd.

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