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Ivanova I.,State Research Institute for Clinical Immunology | Seledtsova G.,State Research Institute for Clinical Immunology | Mamaev S.,Russian Federal Medical and Biological Agency | Shishkov A.,State Research Institute for Clinical Immunology | Seledtsov V.,Immanuel Kant Baltic Federal University
Human Vaccines and Immunotherapeutics | Year: 2014

Patients with rheumatoid arthritis (RA) were treated with a cellular vaccine, which consisted of autologous collagen-reactive T-cells. This study showed that antigen-specific proliferative activity of the peripheral blood mononuclear cells was significantly downregulated after T-cell vaccination in RA patients. T-cell vaccination resulted in a statistically significant decrease in plasma IFNγ levels and a concomitant increase in IL-4 levels in treated patients. Accordingly, following T-cell vaccination the number of IFNγ-producing CD4+ and CD8+ T-cells was decreased by 1.6-1.8-fold, which was paralleled by 1.7-fold increases in IL-4-producing CD4+ T-cells. In addition, the present study showed 5-7-fold increase in the CD8+CD45RO+CD62L- effector memory T-cells and central memory T-cells (both CD4+CD45RO +CD62L+ T-cells and CD8+CD45RO +CD62L+ T-cells) in RA patients, as compared with healthy individuals. We observed significant reduction in CD4+ and CD8 + central memory T-cells, as well as reduction in CD8+ effector memory T-cells in vaccinated patients in the course of the treatment. We also demonstrated that CD4+CD25+FoxP3+ regulatory T-cell levels were significantly up-regulated in the peripheral blood of RA patients following T-cell vaccination. However, CD4+CD25 -FoxP3+ T-cell levels did not significantly change during the entire T-cell vaccination course. In conclusion, the T-cell immunotherapy regimen used resulted in the clinical improvement, which was achieved in 87% patients. © 2014 Landes Bioscience. Source


Kulikov E.E.,Russian Academy of Sciences | Golomidova A.K.,Russian Academy of Sciences | Letarova M.A.,Russian Academy of Sciences | Kostryukova E.S.,Kazan Federal University | And 3 more authors.
Viruses | Year: 2014

Bacteriophage 9g was isolated from horse feces using Escherichia coli C600 as a host strain. Phage 9g has a slightly elongated capsid 62 × 76 nm in diameter and a non-contractile tail about 185 nm long. The complete genome sequence of this bacteriophage consists of 56,703 bp encoding 70 predicted open reading frames. The closest relative of phage 9g is phage PhiJL001 infecting marine alpha-proteobacterium associated with Ircinia strobilina sponge, sharing with phage 9g 51% of amino acid identity in the main capsid protein sequence. The DNA of 9g is resistant to most restriction endonucleases tested, indicating the presence of hypermodified bases. The gene cluster encoding a biosynthesis pathway similar to biosynthesis of the unusual nucleoside queuosine was detected in the phage 9g genome. The genomic map organization is somewhat similar to the typical temperate phage gene layout but no integrase gene was detected. Phage 9g efficiently forms stable associations with its host that continues to produce the phage over multiple passages, but the phage can be easily eliminated via viricide treatment indicating that no true lysogens are formed. Since the sequence, genomic organization and biological properties of bacteriophage 9g are clearly distinct from other known Enterobacteriaceae phages, we propose to consider it as the representative of a novel genus of the Siphoviridae family. © 2014 by the authors; licensee MDPI, Basel, Switzerland. Source


Viktorov A.A.,Applied Research Center for Medical and Ecological Safety | Gladkikh V.D.,Russian Federal Medical and Biological Agency | Ksenofontov A.I.,National Research Nuclear University MEPhI | Morozova E.E.,Russian Federal Medical and Biological Agency
Advances in Gerontology | Year: 2015

A method of iterative congruence for searching for the parameters of a kinetic mathematical model for aging of living systems using medical statistics data is developed. Its possibilities for describing risk functions of mortality and life expectancy for humans and animals depending on environmental factors are illustrated. The concept of forecasting environmental risks, i.e., risks to the population’s health from ecological factors, is formulated. © 2015, Pleiades Publishing, Ltd. Source


Viktorov A.A.,Scientific and Practical Center for Medical and Ecological Safety | Kholodnov V.A.,RAS Institute of Radio Engineering and Electronics | Gladkikh V.D.,Russian Federal Medical and Biological Agency | Alekhnovich A.V.,Russian Federal Medical and Biological Agency
Advances in Gerontology | Year: 2013

A mathematical model simulating the influence of environment (ENV) on aging of living systems is proposed. The model is based on the concept of continuous adaptation of a biological system (BS) to ENV from the moment of its birth. The adaptation rate as the rate of risk of destruction accumulation is regarded as a competition between two concurrent processes-BS destruction and recombination of damages defined by kinetics of autocatalytic chemical reactions. The effect of ENV is taken into account by model parameters, which in general are time-dependent. The model reflects the rules of thermodynamics and gerontology as well as the typical results of medical experiments. © 2013 Pleiades Publishing, Ltd. Source


Takhauov R.M.,Russian Federal Medical and Biological Agency | Karpov A.B.,Russian Federal Medical and Biological Agency | Albach E.N.,Russian Federal Medical and Biological Agency | Khalyuzova M.V.,Tomsk State University | And 14 more authors.
Biopreservation and Biobanking | Year: 2015

Collection and storage of biological specimens in biobanks aims to obtain and preserve samples of different kinds for biological and medical studies. Here we present a description of the Bank of Biological Materials (BBM) housed by the Seversk Biophysical Research Centre (SBRC; Seversk, Russia). The main goal of maintaining the BBM is to collect and store biological samples suitable for genetic studies of people exposed to long-term ionizing radiation. Currently, the collection includes 19,194 biological specimens obtained from 8105 donors, of whom 42.3% are diagnosed with malignant neoplasms, 28.7% are healthy residents of the city of Seversk, 18.8% are healthy employees of the Siberian Group of Chemical Enterprises (SGCE), and 10.2% are patients diagnosed with acute myocardial infarction. The donors were enrolled using the Regional Medical and Dosimetric Register database created by the SBRC. For each donor, DNA specimens were extracted from peripheral blood and tissues and cell suspensions for cytogenetic analysis were prepared routinely. The BBM's unique collection is suitable primarily for studies of individual radiosensitivity of humans (IRH), and genetic aspects of the pathophysiology of common human diseases, especially in populations exposed to long-term low-dose ionizing radiation. © Mary Ann Liebert, Inc. Source

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