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Levashov P.A.,Moscow State University | Matolygina D.A.,Moscow State University | Osipova H.E.,Moscow State University | Savin S.S.,Moscow State University | And 7 more authors.
Moscow University Chemistry Bulletin | Year: 2015

The article continues studies of the recently discovered bacteriolytic activity of interleukin-2. It was detected earlier that interleukin (IL-2) possesses greater substrate specificity in comparison with chicken egg lysozyme. IL-2 disrupted the cell wall of Escherichia coli but did not lyse lysozyme substrates such as the cell walls of Micrococcus luteus and Bacillus subtilis. In the present study it is demonstrated for the first time that both IL-2 and chicken egg lysozyme are capable of lysing Lactobacillus plantarum. The effects of IL-2 and chicken egg lysozyme on Lactobacillus plantarum are compared with those on Escherichia coli. The dependences of the rate of lysis on the concentration of bacteriolytic factors and pH are studied. © 2015, Allerton Press, Inc.

Levashov P.A.,Moscow State University | Ovchinnikova E.D.,Russian Cardiology Research and Scientific Production Complex | Afanas'Eva M.I.,Russian Cardiology Research and Scientific Production Complex | Frid D.A.,Russian Cardiology Research and Scientific Production Complex | And 3 more authors.
Russian Journal of Bioorganic Chemistry | Year: 2015

A new procedure for the preparation of affinity sorbents based on tyramine and tryptamine ligands has been proposed. Sorption characteristics for the developed chromatographic materials were investigated. It was shown that tryptamine-sepharose and tyramine-sepharose efficiently bind IgG, IgA, lipoprotein (a) (Lp(a)) and low density lipoproteins (LDL) from blood plasma. The sorption capacity was 4-9 mg of IgG, 2-4 mg of IgA, 3-5 mg of Lp(a), and 5-7 mg of LDL per mL of gel. It was found that Lp(a) and IgG can bind to the sorbent either as themselves or as a complex of Lp(a) with IgG, the existence of which may indicate the presence of autoantibodies to Lp(a) in some patients. New sorbents are easy in synthesis, stable during use and storage, and they can be used for medical and biotechnological purposes to bind Lp(a), LDL, IgG, and IgA. © 2015 Pleiades Publishing, Ltd.

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