Time filter

Source Type

Shestakova M.V.,Moscow State University | Chazova I.E.,Russian Cardiological Research and Production Complex | Shestakova E.A.,Endocrinology Research Center
Diabetes Mellitus | Year: 2016

Aim. To evaluate the prevalence of undiagnosed type 2 diabetes mellitus (T2DM) among patients with cardiovascular disease. Materials and methods. T2DM screening programs among patients with cardiovascular disease were held from 2013 to 2014 in several Russian cities. In total, 1001 patients aged ≥40 years with hypertension and/or atherosclerotic disease and without prior diagnosis of T2DM were screened in outpatient cardiology clinics. T2DM diagnosis was based on fasting plasma glucose levels, glycated haemoglobin (HbA1c) and/or oral glucose tolerance test (OGTT) results. Blood pressure (BP), family history of T2DM, cardiovascular disease, total cholesterol, low-density lipoprotein (LDL), high-density lipoprotein (HDL) and triglyceride levels were analysed. Results. Fasting glucose was measured in 1000 (99.8%) patients, HbA1c was measured in in 623 (62.2%) and OGTT was performed in 286 (2.6%). Fasting glucose detected 8% of newly diagnosed T2DM; among patients who underwent HbA1c measurement, the prevalence of T2DM was 10.91%, and among patients who underwent OGTT, the prevalence was 13.99%. Depending on the chosen test, the prevalence of undiagnosed pre-diabetes (impaired fasting glycaemia and impaired glucose tolerance) was in the range of 14.4%-36.4%. The majority of patients with T2DM diagnosed by OGTT did not have target blood pressure and lipid levels; 67.5% had elevated systolic BP, 47.5% had elevated diastolic BP, 90.9% had high LDL (≥1.8 mmol/l) and 52.9% had high triglyceride levels (≥1.7 mmol/l). Conclusion. A high prevalence of undiagnosed T2DM (from 8% to 13.99%, depending on the diagnostic criteria) and pre-diabetic state in patients with cardiovascular disease may require screening for T2DM in this high-risk group.


Osidak E.O.,Gamaleya Institute of Epidemiology and Microbiology | Osidak M.S.,Imtek Company | Sivogrivov D.E.,Imtek Company | Portnaya T.S.,Russian Cardiological Research and Production Complex | And 5 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2014

Test system ELISA-BMP-2 is developed for measuring recombinant human bone morphogenetic protein-2 in human and laboratory animal serum and plasma by sandwich ELISA. The test system has been used for studies of the kinetics of bone morphogenetic protein-2 release from collagen carrier in the presence of plasma proteins. © 2014, Springer Science+Business Media New York.


Vanin A.F.,RAS Semenov Institute of Chemical Physics | Chazov E.I.,Russian Cardiological Research and Production Complex
Biophysics | Year: 2011

A stable hypotensive preparation (Oxacom) based on dinitrosyl iron complexes (DNIC) with glutathione has been developed. The preparation has successfully passed pharmacological trials. Tests on volunteers have shown a high hypotensive activity of the preparation: a single intravenous infusion of its aqueous solution at a dose of 0. 2 μmol active substance per kg body wt led to a 20-30% decrease in arterial pressure, which persisted for 15-20 h. Similar experiments on animals demonstrated that aqueous solutions of DNIC with cysteine or glutathione also exert a hypotensive action due to their vasodilatory activity. Besides, these complexes accelerate wound healing and produce a potent erectile effect. There is reason to suppose that DNIC with thiol ligands as NO donors may be cytotoxic for pathogenic mycobacteria Mycobacterium tuberculosis and, after appropriate treatment, inhibit cancer cell proliferation. These complexes can be used as analgesics, for inhibiting the adhesion process, in treating preeclampsia, spermatogenesis pathologies, and in cosmetology for treatment of skin injury. © 2011 Pleiades Publishing, Ltd.


Borodulin R.R.,RAS Semenov Institute of Chemical Physics | Kubrina L.N.,RAS Semenov Institute of Chemical Physics | Shvydkiy V.O.,RAS Semenov Institute of Chemical Physics | Lakomkin V.L.,Russian Cardiological Research and Production Complex | Vanin A.F.,RAS Semenov Institute of Chemical Physics
Nitric Oxide - Biology and Chemistry | Year: 2013

The diamagnetic binuclear form of dinitrosyl iron complexes (B-DNIC) with glutathione can be easily synthesized in the air at ambient temperature. The synthetic protocol includes consecutive addition to distilled water of glutathione, which decreases the pH of the test solution to 4.0, a bivalent iron salt (e.g., ferrous sulphate) and sodium nitrite at the molar ratio of 2:1:1, with a subsequent increase in pH to neutral values. Under these conditions, the amount of B-DNIC formed is limited by initial nitrite concentration. In the novel procedure, 20 mM glutathione, 10 mM ferrous sulfate and 10 mM sodium nitrite give 2.5 mM B-DNIC with glutathione, while 5 mM glutathione remains in the solution. Bivalent iron (5 mM) is precipitated in the form of hydroxide complexes, which can be removed from the solution by passage through a paper filter. After the increase in pH to 11 and addition of thiols at concentrations exceeding that of DNIC tenfold, B-DNIC are converted into a mononuclear EPR-active form of DNIC (M-DNIC) with glutathione. B-DNIC preparations synthesized by using new method contain negligible amount of nitrite or S-nitrosoglutathione as a contaminations. All the steps of DNIC synthesis were characterized by using optical, EPR and HPLC methods. A long-lasting hypotensive action of DNIC formed was demonstrated. © 2013 The Authors. Published by Elsevier Inc. All rights reserved.


Kapelko V.L.,Russian Cardiological Research and Production Complex | Lakomkin V.L.,Russian Cardiological Research and Production Complex | Lukoshkova E.V.,Russian Cardiological Research and Production Complex | Gramovich V.V.,Russian Cardiological Research and Production Complex | And 8 more authors.
Kardiologiya | Year: 2014

Introduction of isoproterenol (an agonist of β-adrenoreceptors) to rats is one of the widespread experimental models of cardiac failure. It is caused by damage of cardiomyocytes with the subsequent development of substitutive fibrosis. The purpose of the given work was the complex characteristic of cardiac function by means of invasive and noninvasive (echocardiography and impedansometry) methods of research. Isoproterenol was injected twice with a daily interval in dozes 85, 120, 150 or 180 mg/kg. Echocardiographic study of the heart in 2 weeks revealed obvious attributes of cardiac failure (left ventricular dilatation, lowered ejection fraction) in the groups which have received high cumulative dozes of isoproterenol (300-360 mg/kg). The catheterization of the left ventricle in these groups has shown raised enddiastolic pressure, decreased maximal rate of pressure development and fall, and also lowered indices of myocardial contractility and relaxability. In the groups which have received smaller isoproterenol dozes, apparent decrease in relaxability parameters (constants of isovolumic and auxovolumic relaxation) has been revealed at only slightly changed parameters of contractility. A strong correlation between echocardiographic and invasive parameters of myocardial contractility has been found. The phase analysis of the cardiac cycle has shown a lengthening of isometric phases of contraction and relaxation, as well as duration of ejection due to shortening duration of filling of both ventricles. Cardiomyocytes isolated from hearts with obvious cardiac failure responded to electrostimulation by arrhythmic contractions and also by much slowed and incomplete removal of free Ca++ from the myoplasm. Results allow to conclude that relatively smaller extent of myocardial damage is accompanied by decreased relaxability at slightly changed contractility, while at greater degree of damage both processes fail, but delay of relaxation still prevails.


Kapelko V.I.,Russian Cardiological Research and Production Complex
Biochemistry (Moscow) | Year: 2012

The rhythm of cardiac beats is generated by pacemaker cells differing from other cardiomyocytes by the presence of slow diastolic depolarization. Consistently activated transmembrane ionic currents provide cyclic excitation of pacemak- ers, forming the original "membrane clocks". A new concept has been forwarded in the last decade according to which peri- odic fluctuations in myoplasmic Ca2+ level ("calcium clocks") not only influence a course of "membrane clocks", but they also can serve as independent generators of the rhythm. Transport of Ca2+ in cells is under constant influence of active forms of oxygen and nitrogen. Both superoxide and NO in moderate doses facilitate Ca2+ output from the sarcoplasmic reticulum, accelerating the course of "calcium clocks", but in higher doses they have opposite effect that may be neutralized mainly by reduced glutathione. The control of cardiac rhythm by active forms of oxygen and nitrogen represents a feedback mechα- nism by which mitochondria and NO-synthases support Ca2+ homeostasis in cells that can be temporarily disturbed under mechanical loads or hypoxia. © Pleiades Publishing, Ltd., 2012.


Spiridonova V.A.,Moscow State University | Levashov P.A.,Moscow State University | Ovchinnikova E.D.,Russian Cardiological Research and Production Complex | Afanasieva O.I.,Russian Cardiological Research and Production Complex | And 3 more authors.
Russian Journal of Bioorganic Chemistry | Year: 2014

DNA aptamer-based sorbents were synthesized for binding human IgE. Sorbents effectively removed IgE from human blood plasma. The experimental values of IgE desorption constants were within the range of 1.1 × 10-10 to 1.7 × 10-10 M depending on the orientation of the aptamer on an insoluble matrix. The sorbents were stable during multiple use. Conditions for sorbent regeneration were selected. These chromatographic materials can be used for medical and biotechnological applications. © 2014 Pleiades Publishing, Ltd.


Sidorova M.V.,Russian Cardiological Research and Production Complex | Arefieva T.I.,Russian Cardiological Research and Production Complex | Palkeeva M.E.,Russian Cardiological Research and Production Complex | Molokoedov A.S.,Russian Cardiological Research and Production Complex | And 5 more authors.
Russian Journal of Bioorganic Chemistry | Year: 2015

The automated Fmoc solid-phase technique was used to synthesize Cys-containing linear peptide fragments of monocyte chemoattractant protein-1, chemokine domain of fractalkine, and their analogues, with the Cys residue being either modified or replaced with Ser. Chimeric symmetric and asymmetric disulfides were also prepared from the linear precursors. A SAR study of a set of the newly synthesized peptides showed that the capacity to stimulate migration of monocytes and influence cell motility in vitro critically depends, in general, on the presence of free Cys thiol group in the molecule. Notably, all analogues, including chimeric disulfides, containing no SH groups demonstrated the lack of chemokinetic properties. © 2015 Pleiades Publishing, Ltd.


PubMed | Russian Cardiological Research and Production Complex
Type: | Journal: Clinical drug investigation | Year: 2016

Blood pressure (BP) control in hypertensive patients remains poor worldwide, particularly in high-risk patients with hypertension and diabetes. Guidelines recommend that such patients receive prompt pharmacological therapy at maximal doses to rapidly control BP. We aimed to evaluate efficacy and safety of single-pill combination (SPC) perindopril/indapamide (PER/IND) at full dose (10/2.5mg) in hypertensive patients, including diabetics, with BP uncontrolled by previous medication.Twelve-week prospective, observational study in patients with uncontrolled hypertension (160-200mmHg systolic BP [SBP] and <110mmHg diastolic BP [DBP]) on a previous SPC or free-dose combination of renin-angiotensin system blocker plus thiazide diuretic, substituted with PER/IND 10/2.5mg. Office BP, quality of life, and blood parameters were evaluated in the whole cohort and patients with type 2 diabetesmellitus.2120 ambulatory hypertensive patients were enrolled, including 307 with type 2 diabetes. Two weeks after substitution, SBP significantly decreased from 171.013.3 to 148.613.4mmHg, and DBP from 98.68.3 to 88.87.9mmHg (both p<0.00001). A similar rapid decrease was noted in the diabetes subgroup. After 12weeks, BP had reduced by 42/19mmHg in the whole cohort (diabetes subgroup: 41/18mmHg). Most (84%; diabetes subgroup: 77%) patients reached BP target (<140/90mmHg). Laboratory tests and quality of life improved in the whole cohort and the diabetic subgroup.Switching to PER/IND at full dose (10/2.5mg) was well tolerated, leading to fast BP reduction and control in the majority of patients with uncontrolled hypertension, including difficult-to-treat patients with diabetes.


PubMed | Russian Cardiological Research and Production Complex
Type: Journal Article | Journal: Biochemistry. Biokhimiia | Year: 2012

The rhythm of cardiac beats is generated by pacemaker cells differing from other cardiomyocytes by the presence of slow diastolic depolarization. Consistently activated transmembrane ionic currents provide cyclic excitation of pacemakers, forming the original membrane clocks. A new concept has been forwarded in the last decade according to which periodic fluctuations in myoplasmic Ca2+ level (calcium clocks) not only influence a course of membrane clocks, but they also can serve as independent generators of the rhythm. Transport of Ca2+ in cells is under constant influence of active forms of oxygen and nitrogen. Both superoxide and NO in moderate doses facilitate Ca2+ output from the sarcoplasmic reticulum, accelerating the course of calcium clocks, but in higher doses they have opposite effect that may be neutralized mainly by reduced glutathione. The control of cardiac rhythm by active forms of oxygen and nitrogen represents a feedback mechanism by which mitochondria and NO-synthases support Ca2+ homeostasis in cells that can be temporarily disturbed under mechanical loads or hypoxia.

Loading Russian Cardiological Research and Production Complex collaborators
Loading Russian Cardiological Research and Production Complex collaborators