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Gurskii Y.G.,Russian Cardiological Research and Industrial Complex for Russian Medical Technologies | Minashkin M.M.,Russian Cardiological Research and Industrial Complex for Russian Medical Technologies | Feoktistova E.S.,Russian Cardiological Research and Industrial Complex for Russian Medical Technologies | Skamrov A.V.,Russian Cardiological Research and Industrial Complex for Russian Medical Technologies | And 2 more authors.
Applied Biochemistry and Microbiology | Year: 2010

A recombinant plasmid carrying a modified gene of human plasminogen (mini-plasminogen), lacking four kringle domains and an amino terminal fragment, and containing an additional oligopeptide of six N-terminal histidine residues has been constructed. The plasmid was used for transformation of E. coli JM 109 cells to obtain a strain producing a recombinant modified human plasminogen. The target protein is superexpressed in a form of inclusion bodies and is composed of more than 50% insoluble protein. The renaturated and chromatographically purified protein exhibits amidolytic activity specific for plasminogen proenzyme in a fibrinolytic system. © 2010 Pleiades Publishing, Ltd. Source

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