Russian Cardiologic R and D Production Complex of Minzdrav of Russia

Moscow, Russia

Russian Cardiologic R and D Production Complex of Minzdrav of Russia

Moscow, Russia
SEARCH FILTERS
Time filter
Source Type

Titov V.N.,Russian Cardiologic R and D Production Complex of Minzdrav of Russia
Klinichescheskaya Laboratornaya Diagnostika | Year: 2016

The content of fatty acids-binding protein in blood serum increases in 2-3 hours after acute coronary syndrome and myocardium infarction and reaches its maximal value after 8/5 hours in average after beginning of cardiomyocytes destruction. To the end of the first day of acute coronary syndrome the content of fatty acids-binding protein in blood significantly decreases due to excretion with urine. The level of fatty acids-binding protein in blood during a day remains slightly higher than initial one. The high clinical sensitivity and relatively high organ specificity is characteristic for fatty acids-binding protein in 12 hours after acute coronary syndrome. In early terms ofmyocardium infarction clinical specificity of fatty acids-binding protein is prevailed over concentration of troponins. In 12 hours after acute coronary syndrome troponins begin to manifest higher clinical and sensitivity and diagnostic specificity that dominate during several days of myocardium infarction. Under simultaneous measurement of fatty acids-binding protein and troponins sensitivity and of biochemical diagnostic increases up to 30% during first 12 hours. In later time periods advantage of simultaneous detection of fatty acids-binding protein and troponins stops to be necessary. At this time, fatty acids-binding proteins are evacuated through kidneys with urine excretion. After 12 hours the diagnostic significance of detection of troponins becomes dominating. No relationship between was established between content and of fatty acids-binding proteins in blood serum and indications of reperfusion of myocardium according electrocardiogram data. The possible cause is probably related to rare blood sampling for detection of fatty acids-binding protein such a dynamic test. The test doesn't do for evaluating the condition of patients with circulatory deficiency because of results not enough adequate. The best alternative of differentiate diagnostic during first day of acute coronary syndrome would be application of combined express immune chromatographic test permitting "at bedside of patient" or in ambulance car to dynamically and simultaneously detect fatty acids-binding proteins and troponins in whole blood and "to preserve " the results up to objective assessment.


For billions years, two phylogenelically, functionally and regulatory different pools of fatty cells - visceral fatty acids and adipocytes-coexist in vivo. Their becoming occurred at different degrees of phylogenesis. The phylogenelically earlier pool of visceral fatty acids is meant to supply with fait)' acids-substrates for gaining energy' by those cells which implement biological function of nutrition (trophology), homeostasis, endoecology, biological function of adaptation and continuation of species. They have no receptors to phylogenelically later insulin. The adipocytes, later in phylogenesis, implement one biological function-the function of locomotion and they are as insulin-dependent as skeletal myocytes, cardiomyocytes, adipocytes and periportal hepatocytes. The difference in regulation is traced on all levels of "biological perfection " - autocrine (cellular) level, in humoral regulated paracrin cenosises of cells and on the level of organism. In biological function of trophology, paracrin cenosises of visceral fatty acids and adipocytes implement subsequently three biological reactions: exolrophy, deposit of fatty acids and endotrophy. In conditions of humoral regulation of three functionally different biological reactions in paracrin cenosises synthesis of so many humoral mediators is required. The humoral mediators of mechanism of feedback at autocrine level, in paracrin cenosises and at the level of organism are leptin of visceral fatty acids and adiponectin of adipocytes. At the level of organism, phylogenelically earlier paracrin cenosises of fatty cells are regulated by endocrine system. The phylogenelically later paracrin cenosises are regulated by insulin and nuclei of hypothalamus. The metabolic syndrome is a pathology of phylogenelically earlier insulin-independent visceral fatty acids. The obesity is a pathology of phylogenelically later pool of insulin-dependent adipocytes.


Titov V.N.,Russian Cardiologic R and D Production Complex of Minzdrav of Russia
Klinichescheskaya Laboratornaya Diagnostika | Year: 2016

In phylogenesis, becoming of biological functions and biological reactions proceeds with the purpose of permanent increasing of "kinetic perfection ". The main role belongs to factors of physical, chemical and biological kinetics, their evaluation using systemic approach technique under permanent effect of natural selection. The late-in-phylogenesis insulin, proceeded with, in development of biological function of locomotion, specialization of insulin-dependent cells: skeletal myocytes, syncytium of cardiomyocytes, subcutaneous adipocytes, periportal hepatocytes, Kupffer's macrophages and ß-cells of islets of pancreas. The insulin initiated formation of new, late in phylogenesis, large pool of fatty cells-subcutaneous adipocytes that increased kinetic parameters of biological function of locomotion. In realization of biological function of locomotion only adipocytes absorb exogenous mono unsaturated and saturated fatty acids in the form of triglycerides in composition of oleic and palmitic lipoproteins of very low density using apoE/B-100 endocytosis. The rest of insulin-dependent cells absorb fatty acids in the form of unesterified fatty acids from associates with albumin and under effect of CD36 of translocase of fatty acids. The insulin in all insulin-depended cells inhibits biological reaction of lipolysis enhancing contributing into development of lipoidosis. The insulin expresses transfer of fatty acids in the form of unsaturated fatty acids from adipocytes into matrix of mitochondria. The insulin supplies insulin-dependent cells with substrates for acquiring energy subject to that in pool of unsaturated fatty acids in adipocytes prevails hydrophobic palmitic unsaturated fatty acid that slowly passes into matrix through external membrane of mitochondria; oxidases of mitochondria so slowly implement its ß-oxidation that content of exogenous palmitic unsaturated fatty acid can't be higher than phylogenetic, physiological level -15% of all amount of fatty acids transferring to insulin-dependent cells. The insulin can't both to decrease content of exogenous palmitic fatty acid and inhibit lipolysis in visceral fatty cells of omentum.


Titov V.N.,Russian Cardiologic R and D Production Complex of Minzdrav of Russia
Klinichescheskaya Laboratornaya Diagnostika | Year: 2014

The decentralized system of resident macrophages phylogenetically is earlier and complement-depending one in every paracrin regulated cenosis of cells, intima of elastic type arteries. This system primarily utilizes protein macromolecules implementing biological reaction of transcytosis. The anatomically andfunctionally more perfect system of insulin-depended Kupffer macrophages in liver is centralized at the level of organism and is also intended to collect and utilize minor and protein biological "garbage ". The various peptides, humoral active mediators, fragments of plasmatic membranes, integral proteins of micro RNA in hydrophilic medium of blood plasma are forming under their physical chemical interaction micro-particles, micro-vesicles and exosomes. All of them, under effect of IgG, absorb phylogenetically late Kupffer macrophages. The consequent system of implementation of biologic function of endoecology includes biologic reaction of exocytosis at autocrin level; complement-depended macrophages in paracrin cenosises of cells; resident macrophages in intima of elastic type arteries with reaction of transcytosis; centralized Kupffer macrophages in liver in sinusoidal capillaries and Disse spaces without reaction of transcytosis. The difference of function of systems makes it possible to make a conception of the role of biologic function of endoecology in pathological processes. Therefore, an opportunity appears to evaluate diagnostic value of methods based on detection of amount and quality composition of micro particles of blood plasma. This can be useful in differential diagnostic of metabolic pandemics.


Titov V.N.,Russian Cardiologic R and D Production Complex of Minzdrav of Russia
Klinichescheskaya Laboratornaya Diagnostika | Year: 2013

The formation of function of isozymes of stearil-coenzymeA-desaturases occured at the different stages of phytogeny under realization of biologic function of trophology (stearil-coenzymeA-desaturase 1) and biologic function of locomotion, insulin system (stearil-coenzymeA-desaturase 2) billions years later. The stearil-coenzymeA-desaturase 1 transforms in C 18:1 oleic fatty acid only exogenous C 16:0 palmitinic saturated fatty acid. The stearil-coenzymeA- desaturase 2 transforms only endogenic palmitinic saturated fatty acid, synthesized form glucose. The biologic role of insulin is in energy support of biologic function of locomotion. Insulin through expressing stearil-coenzymeA- desaturase 2 transforms energetically non-optimal palmitinic variation of metabolism of substrates into highly effective oleic variation for cells' groundwork of energy (saturated fatty acid and mono fatty acid). The surplus of palmitinic saturated fatty acid in food is enabled in pathogenesis of resistance to insulin and derangement of synthesis of hormone byβ-cells of islets. The resistance to insulin and diabetes mellilus are primarily the derangement of metabolism of saturated fatty acids with mono fatty acids, energy problems of organism and only afterwards the derangement of metabolism of carbohydrates. It is desirable to restrict food intake of exogenous palmitinic saturated fatty acid. The reasons are low expression of independent of insulin stearil-coenzymeA-desaturase 2, marked lipotoxicity of polar form of palmitinic saturated fatty acid and synthesis of non-optimal palmitinic triglycerides instead of physiologic and more energetically more effective oleic triglycerides.


Every cell reserves fatty acids in cytozol in drops of lipids in the form of non-polar triglycerides for itself and for oxidation in mitochondria. The specialized visceral fatty cells of omentum and adipocytes of subcutaneous fat are the cells absorbing saturated and mono unsaturated fatty acids inform of triglycerides in apoB-48 chylomicrons, apoB-100 lipoproteins of low and very low density. They deposit their physiological time and liberate fatty acids in intercellular medium in the form of polar unesterified fatty acids bound by albumin. According phylogenetic theory of general pathology, in biological function of trophology (nutrition) fatty cells sequentially implement biological reaction of exotrophy (external nutrition), deposition and endotrophy (internal nutrition). The humoral regulator of feedback in visceral fatty cells is leptin acting in autocrine way, in paracrin cenosises of cells and on the level of organism. The biological role of leptin is in preventing a) deposition of surplus amount of non-polar triglycerides in fatty cells; b) formation of endoplasmic "stress"; c) death of fatty cells in apoptosis way, formation of corpuscles of apoptosis and failure of biological function of endoecology; d) formation of biological reaction of inflammation in visceral fatty tissue; e) high level of unsaturated fatty acids in intercellular medium and J) development of metabolic syndrome. The leptin prevents aphysiological deposit of non-polar triglycerides in insulin-dependent cells that are not intended to deposit non-polar triglycerides and also in β-cells of islands. The main cause of high level of leptin in blood plasma is overeating of food physiological by content of nutrients.


Titov V.N.,Russian Cardiologic R and D Production Complex of Minzdrav of Russia
Klinichescheskaya Laboratornaya Diagnostika | Year: 2014

The first function of carrier protein of cholesterol ethers in phylogenesis was passive transfer of polar diglycerides from lipoproteins of very low density to lipoproteins of very high density to proceed with hydrolysis. At the later degrees, carrier protein of cholesterol ethers initiated reverse transfer of polyenoic fatty acids from lipoproteins of very high density to lipoproteins of very low density inform of polyenoic ethers with spirit cholesterol. This occurrence became a stage of transfer and active absorption by cells polyenoic fatty acids using apoB-100 receptor endocytosis in lipoproteins of very low density. Later on, in phylogenesis under mutation of carrier protein of cholesterol ethers null cells began compensatory absorb polyenoic fatty acids in lipoproteins of very high density by force of apoE/A-1 endocytosis. In certain percentage of species of animals (primates, rabbits) and humans the cells absorb polyenoic fatty acids by force of apoB-100 endocytosis. In other species (rats and mice) the cells absorb polyenoic fatty acids through apoE/A=I receptors. In animals, under first receptor absorption of polyenoic fatty acids by cells it is easy to reproduce atherosclerosis and atheromatosis on the model of exogenous hypercholesterolemia and it is practically impossible in second case. The absence in vivo of lipoproteins of very high density and triple associate of lipoproteins of very high density + carrier protein of cholesterol ethers + lipoproteins of very low density is followed by increasing of spirit cholesterol-lipoproteins of very high density and decreasing of content of spirit cholesterol-lipoproteins of very low density as risk factors of human atherosclerosis. This occurrence served as foundation for proposal to apply blockers of action of carrier protein of cholesterol ethers in patients with atherosclerosis. The main function of lipoproteins of very high density as of all lipoproteins is to transfer fatty acids to cells and only in second instance to transfer from all cells spirit cholesterol in the form of mono-ethers cholesterol. Only results of clinical studies will demonstrate the effectiveness of compensatory apoE/A-I receptor endocytosis of lipoproteins of very low density in comparison with physiological apoB-100 endocytosis.


Titov V.N.,Russian Cardiologic R and D Production Complex of Minzdrav of Russia
Klinichescheskaya Laboratornaya Diagnostika | Year: 2015

The phylogenetic theory of general pathology postulates that in physiology and pathology the concepts of biological role of arterial tension had been subjected to inversion. The activation by nephron of synthesis of components rennin-angiotensin II and increasing of aldosterone secretion are directed not to increase arterial tension but to preserve volume of piece of third world ocean privatized by each entity as pool of intercellular medium where all cells continue to live as billions years before. In phylogenetic sense, early organs can't regulate effect of physical factor of regulation of metabolism the late one in phylogenesis of arterial tension. The cause of increasing of arterial tension is the vasomotor center but not the kidneys. The vasomotor center increases arterial tension in the proximal section and further hydrodynamic tension in the distal section of arterial stream and tends to resuscitate function of nephrons, biological function of endoecology and biological reaction of excretion. The arterial tension, besides the main role in biological function of locomotion, is a physical factor of compensation of disorders of biological functions of homeostasis, trophology, endoecology and adaptation. In phylogenesis, three levels of metabolism regulation has been developed. The specific regulation ofbiochemical reactions occurs on autocrine level. In paracrin regulated cell cenosises, at distal section of arterial stream, metabolism is regulated by billions of local peristaltic pumps through compensation of biological reaction of endoiheliiun-depended vasodilatation, micro-circulation, effect of humoral mediators and hormonal principles. In vivo, from the level of vasomotor center, metabolism non-specifically and systemic regulates physical factor-arterial tension through sympathetic activation of heart. The arterial tension in proximal section of arterial stream overcomes resistance and physically "forces through" arterioles with disordered micro-circulation. The angiotensin If is a vasoconstrictor only in distal section of arterial stream. In the process of pathogenesis of essential metabolic arterial hypertension the paracrin cenosises of nephron and kidneys are secondary involved into realization of pathological compensation and very often are "guilty without guilt" as such another "target organs" as brain, lungs and heart as well.


Titov V.N.,Russian Cardiologic R and D Production Complex of Minzdrav of Russia
Klinichescheskaya Laboratornaya Diagnostika | Year: 2014

The becoming of visceral fatty cells in phylogenesis occurred many billions years before subcutaneous adipocytes. The pool of visceral fatty cells realizes biologic functions of trophology and homeostasis, endoecology and adaptation from the early stages of phylogenesis. The subcutaneous adipocytes realize phylogenetically late biologic function of locomotion. The visceral fatty cells have no receptors to insulin and all subcutaneous adipocytes are insulin-dependent. In biologic function of trophology, both visceral fatty cells and subcutaneous adipocytes realize biologic reaction of exotrophy, biologic reaction of depositing and biologic reaction of endotrophy. It is supposed that the most common cause of obesity is disorder of biologic reaction of depositing of fatty acids inform of triglycerides. It is considered as a basis of that dysfunction of visceral fatly cells (metabolic syndrome) and insulin-dependent adipocytes (obesity) takes so often a character of metabolic pandemic. The fatly cells absorb fatly acids in form of non-polar triglycerides, deposit them in lipid drops and free fatty acids into intercelluar medium in form of polar unesterified fatty acids. The visceral fatly cells had been formed in paracrin cenosis of enterocytes and in it microsome protein transferring triglycerides formed early type of chylomicrons. The visceral fatly cells and adipocytes are phylogenetically, regulatory, functionally and pathophysiologically different cells. Therefore, they are to be considered separately. Not only visceral fatty cells and adipocytes but all cells of areolar tissue at level of cenosis of cells secrete many humoral mediators of paracrin regulation. The other modes of regulation are unknown. Leptin is a specific mediator of visceral fatty cells and adiponectin is a mediator of subcutaneous adipocytes.


After more than half-century of different conceptions, the theory of general pathology was used to substantiate that all lipoproteins are bi-layer: lipid by their structure. The main function of high density lipoproteins as of all lipoproteins is transfer of fatty acids to cells and only in second turn taking away of spirit cholesterol from cells. At the stages of phylogenesis high density lipoproteins, low density lipoproteins and very low density lipoproteins began to function in a subsequent way. The fatty acids were transferred by low density lipoproteins in polar lipids at passive absorption by cells. Later on, lipoproteins transfer fatty acids in non-polar ethers with spirits glycerin and spirit cholesterol. The cells absorb them by receptor endocytosis. The hepatocytes secret in blood palmitic, oleic, linoleic and linoleic very low density lipoproteins. The palmitic and oleic very low density lipoproteins absorb physiologically insulin-dependent cells apoE/B-100=endocytosis. The linoleic and linoleic very low density lipoproteins after transition of polyether s cholesterol from high density lipoproteins turn into low density lipoproteins. The cells absorb them by apoB-100=endocytosis. The formation of chylomicrons occurs in blood and hepatocytes absorb them by the way of apoB/E-48=endocytosis. The absorption of poly-unsaturated fatty acids by cells with apoB-100=endocytosis form sensitivity of animals to exogenous hyper spirit cholesterol and absorption of poly-unsaturated fatly acids by apoE/A-l=receptors form corresponding resistance. The ApoE in lipoproteins form cooperative ligands - apoE/B-48 for chylomicrons, apoE/B-100 for very low density lipoproteins and apoE/A-I for high density lipoproteins. The chylomicrons in blood form apoB-48 from complexes of triglycerides secreted by enterocytes. These views change conceptions of pathogenesis and prevention of atherosclerosis, metabolic syndrome and resistance to insulin whose pathogenesis is unified by disorder of transfer in intercellular medium and absorption of fatty acids by cells.

Loading Russian Cardiologic R and D Production Complex of Minzdrav of Russia collaborators
Loading Russian Cardiologic R and D Production Complex of Minzdrav of Russia collaborators