Shlyakhtin S.V.,RUE Belmedpreparaty |
Trukhacheva T.V.,RUE Belmedpreparaty |
Isakov G.A.,RUE Belmedpreparaty
Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry | Year: 2010
A precise and reproducible method of quantitative determination of the photosensitizer (PS) Photolon in liver samples of laboratory animals by means of a spectrophotometric assay with preliminary extraction has been developed. Conditions of the PS extraction have been optimized and validated for the quantitative determination of the PS Photolon by the spectrophotometric assay and the main analytical characteristics have been investigated. Using the method of quantitative determination of the PS Photolon in liver tissue samples a quantitative estimation of in vivo fluorescence of the liver tissue was performed after PS administration to animals. There was a high correlation (R = 0.99) between results obtained by spectrophotometry ex vivo and spectrofluorimetry in vivo. The method of fluorescence detection in vivo is applicable for studies of the pharmacokinetics of different photosensitizers. © 2010 Pleiades Publishing, Ltd.
Istomin Y.P.,National Cancer Center Republic of Belarus |
Lapzevich T.P.,National Cancer Center Republic of Belarus |
Chalau V.N.,National Cancer Center Republic of Belarus |
Shliakhtsin S.V.,RUE Belmedpreparaty |
Trukhachova T.V.,RUE Belmedpreparaty
Photodiagnosis and Photodynamic Therapy | Year: 2010
The objective of the present study was to test in clinics a previously developed novel organ-saving approach for the treatment of CIN using PDT with the photosensitizer Photolon® applied in women of a childbearing age with CIN II and III. A total number of 112 patients aged 35.2±1.6 with morphologically proven diagnosis of CIN II and III were enrolled into the study. All 112 patients had been observed at least during 1-year follow-up period after PDT. Among them 53 patients (44.1%) were subjected to a dynamic observation for less than 2 years; 29 patients (24.1%) were under the observation for less than 3 years; 13 patients (10.8%) - for 3-4 years and 17 women - for more than 4 years. A complete response represented by the complete regression of neoplastic lesions, which was proved by the results of morphological examinations, was revealed in 104 (92.8%) of treated women. In 3 months after treatment a complete eradication of the HPV infection was proven by PCR-analysis in 47 (53.4%) from 88 patients who have been infected with HPV of a highly oncogenic strains before PDT. PDT with Photolon® is an alternative approach for the treatment of cervical intraepithelial neoplasia which can be recommended for women of childbearing age. The simplicity of the procedure as well as its' high therapeutic efficacy defines the reasonability of its' introduction into the clinical practice as a new organ-saving method for the treatment of patients with cervical intraepithelial neoplasia. © 2010 Elsevier B.V.