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Huang G.Q.,Rubber Research Institute of China
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji | Year: 2010

In order to enrich gene encoding region of Hevea brasiliensis, a methylation filtration library was constructed using Escherichia coli McrBC restriction-modification system. The titers of the non-amplified library and the amplified library were 2.6×106 pfu/ml and 9.0×109, respectively. The rate of positive clones was 86.4%. The lengths of inserted DNA sequence ranged from 1 kb to 2.5 kb and the average size of inserts was 1.2 kb. One hundred clones were selected randomly for sequencing, resulting in splicing out of 81 non-redundant sequences, including 6 contigs and 75 singlets. The redundancy was 17.35%. Blast analysis showed that 39.5% of non-redundant sequences were homologous with the Nr database, 14.81% with the EST database, and 32.1% were unknown sequences. Some sequences were related genes for flowering, insect and disease resistance. Therefore, the rubber tree methylation library is helpful for discovery and cloning of functional genes. Source

Zhao L.,Jilin University | Zhao L.,Rubber Research Institute of China | Yao H.,Jilin University | Liu Y.,Jilin University | And 2 more authors.
Journal of Applied Polymer Science | Year: 2013

A fluorinated hyperbranched polyimide (HBPI) is synthesized by using a triamine monomer, 1,3,5-tris(2-trifluoromethyl-4-aminophenoxy)benzene (TFAPOB) (B3), as a "core" molecule, 4,4′-oxydiphthalic anhydride (ODPA) as a A2 monomer, and 4-aminophthalonitrile as an end-capping reagent. After that, a series of novel fluorinated hyperbranched polyimides end-capped with metallophthalocyanines were prepared by the reactions of dicyanophenyl end-capped hyperbranched polyimide with excessive amounts of 1,2-dicyanobenzene and the corresponding metal salt in quinoline. The resulting polyimides containing metallophthalocyanine unites shows optical absorption in the visible region. The absorption bands of the polymers in chloroform solution are in the range of 665-701 nm. These polyimides show glass transition temperatures between 216 and 225°C, and the 5 wt % weight loss temperature of the polymers varied from 440 to 543°C under nitrogen. © 2012 Wiley Periodicals, Inc. Source

Chen Q.B.,Rubber Research Institute of China | Chen Q.B.,Chinese Academy of Sciences
Acta Horticulturae | Year: 2013

China is one of the 94 countries producing mango in the world. Cultivation of mango in China dates back to 1000 years ago. However, large commercial production of mango did not begin until the 1960s, particularly the 1980s. In mainland China, Hainan Province is the largest producer of mango, followed by Guangxi Autonomous Region, Guangdong Province, Yunnan Province, Sichuan Provinces and Fujian Province. The area under mango trees in mainland China is estimated about 120,000 ha in 2008 with the total production of 800,000 MT. Some 260 MT of the mango output in 2008 was exported while over 20,000 MT imported. Therefore China is rather a big mango consuming country than a producing country. China's import of mango is chiefly in the form of fresh mango fruit mainly from the Philippines, Thailand, Vietnam, Indonesia, Myanmar, Australia, India, Peru and Pakistan. Although China possesses endemic wild mango species, most of the mango cultivars are exotic origin, including Carabao, Nang Klangwan, Dashehari, Okrong, Irwin, Haden, etc. National and regional research institutions and universities in mainland China have made great efforts in selection and breeding programme and cultural practices for high quality and higher return mango production by challenging various unfavorable environmental constraints and pests and diseases. © ISHS 2013. Source

Liu J.-P.,Hainan University | Xia Z.-Q.,Chinese Academy of Sciences | Tian X.-Y.,Hainan University | Li Y.-J.,Rubber Research Institute of China
BMC Genomics | Year: 2015

Background: Tapping panel dryness (TPD) involves in the partial or complete cessation of latex flow thus seriously affect latex production in rubber tree (Hevea brasiliensis). Numerous studies have been conducted to define its origin and nature, but the molecular nature and mechanism of TPD occurrence remains unknown. This study is committed to de novo sequencing and comparative analysis of the transcriptomes of healthy (H) and TPD-affected (T) rubber trees to identify the genes and pathways related to the TPD. Results: Total raw reads of 34,632,012 and 35,913,020 bp were obtained from H and T library, respectively using Illumina Hiseq 2000 sequencing technology. De novo assemblies yielded 141,456 and 169,285 contigs, and 96,070 and 112,243 unigenes from H and T library, respectively. Conclusions: In TPD-affected trees, the expression of most genes related to the latex biosynthesis and jasmonate synthesis was severely inhibited and is probably the direct cause of the TPD. These new de novo transcriptome data sets provide a significant resource for the discovery of genes related to TPD and improve our understanding of the occurrence and maintainace of TPD. © 2015 Liu et al.; licensee BioMed Central. Source

Dai L.-J.,Rubber Research Institute of China | Zeng R.-Z.,Rubber Research Institute of China
Zhiwu Shengli Xuebao/Plant Physiology Journal | Year: 2013

Rubber transferase, a cis-prenyltransferase catalyzing the incorporation of isopentenyl pyrophosphate into rubber molecules, is critical to the quality and the yield of natural rubber. However, the identity of the rubber transferase, and the mechanisms related to polymerization and the determination of the molecular mass of natural rubber remain equivocal. This article provides an overview of the rubber transferases of Hevea brasiliensis and other rubber-producing plants from aspects of the biological function, the purification, the characterization, and the regulation of the catalytic activity. Source

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