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Wang L.,Beijing Institute of Technology | Chen X.,Beijing Institute of Technology | Yang K.,Beijing Institute of Technology | Yang K.,Rubber Research Institute of China | Jin H.,Beijing Institute of Technology
Proceedings of SPIE - The International Society for Optical Engineering | Year: 2017

Batch processes are always characterized by nonlinear and system uncertain properties, therefore, the conventional single model may be ill-suited. A local learning strategy soft sensor based on variable partition ensemble method is developed for the quality prediction of nonlinear and non-Gaussian batch processes. A set of input variable sets are obtained by bootstrapping and PMI criterion. Then, multiple local GPR models are developed based on each local input variable set. When a new test data is coming, the posterior probability of each best performance local model is estimated based on Bayesian inference and used to combine these local GPR models to get the final prediction result. The proposed soft sensor is demonstrated by applying to an industrial fed-batch chlortetracycline fermentation process. © 2017 SPIE.


PubMed | Rubber Research Institute of China and CAS Beijing Institute of Genomics
Type: Journal Article | Journal: FEBS open bio | Year: 2017

Calcium-dependent protein kinases (CDPKs or CPKs) play important roles in various physiological processes of plants, including growth and development, stress responses and hormone signaling. Although the CDPK gene family has been characterized in several model plants, little is known about this gene family in


Liu J.-P.,Hainan University | Xia Z.-Q.,Chinese Academy of Sciences | Tian X.-Y.,Hainan University | Li Y.-J.,Rubber Research Institute of China
BMC Genomics | Year: 2015

Background: Tapping panel dryness (TPD) involves in the partial or complete cessation of latex flow thus seriously affect latex production in rubber tree (Hevea brasiliensis). Numerous studies have been conducted to define its origin and nature, but the molecular nature and mechanism of TPD occurrence remains unknown. This study is committed to de novo sequencing and comparative analysis of the transcriptomes of healthy (H) and TPD-affected (T) rubber trees to identify the genes and pathways related to the TPD. Results: Total raw reads of 34,632,012 and 35,913,020 bp were obtained from H and T library, respectively using Illumina Hiseq 2000 sequencing technology. De novo assemblies yielded 141,456 and 169,285 contigs, and 96,070 and 112,243 unigenes from H and T library, respectively. Conclusions: In TPD-affected trees, the expression of most genes related to the latex biosynthesis and jasmonate synthesis was severely inhibited and is probably the direct cause of the TPD. These new de novo transcriptome data sets provide a significant resource for the discovery of genes related to TPD and improve our understanding of the occurrence and maintainace of TPD. © 2015 Liu et al.; licensee BioMed Central.


Zhao L.,Jilin University | Zhao L.,Rubber Research Institute of China | Yao H.,Jilin University | Liu Y.,Jilin University | And 2 more authors.
Journal of Applied Polymer Science | Year: 2013

A fluorinated hyperbranched polyimide (HBPI) is synthesized by using a triamine monomer, 1,3,5-tris(2-trifluoromethyl-4-aminophenoxy)benzene (TFAPOB) (B3), as a "core" molecule, 4,4′-oxydiphthalic anhydride (ODPA) as a A2 monomer, and 4-aminophthalonitrile as an end-capping reagent. After that, a series of novel fluorinated hyperbranched polyimides end-capped with metallophthalocyanines were prepared by the reactions of dicyanophenyl end-capped hyperbranched polyimide with excessive amounts of 1,2-dicyanobenzene and the corresponding metal salt in quinoline. The resulting polyimides containing metallophthalocyanine unites shows optical absorption in the visible region. The absorption bands of the polymers in chloroform solution are in the range of 665-701 nm. These polyimides show glass transition temperatures between 216 and 225°C, and the 5 wt % weight loss temperature of the polymers varied from 440 to 543°C under nitrogen. © 2012 Wiley Periodicals, Inc.


PubMed | Rubber Research Institute of China, CAS Xishuangbanna Tropical Botanical Garden and CAS Kunming Institute of Zoology
Type: | Journal: Frontiers in plant science | Year: 2017

Having been introduced to the northern edge of Asian tropics, the rubber tree (


Dai L.,Key Laboratory of Biology and Genetic Resources of Rubber Tree | Dai L.,Rubber Research Institute of China | Kang G.,Key Laboratory of Biology and Genetic Resources of Rubber Tree | Kang G.,Rubber Research Institute of China | And 8 more authors.
Plant Molecular Biology | Year: 2013

The rubber particle is a special organelle in which natural rubber is synthesised and stored in the laticifers of Hevea brasiliensis. To better understand the biological functions of rubber particles and to identify the candidate rubber biosynthesis-related proteins, a comprehensive proteome analysis was performed on H. brasiliensis rubber particles using shotgun tandem mass spectrometry profiling approaches-resulting in a thorough report on the rubber particle proteins. A total of 186 rubber particle proteins were identified, with a range in relative molecular mass of 3.9-194.2 kDa and in isoelectric point values of 4.0-11.2. The rubber particle proteins were analysed for gene ontology and could be categorised into eight major groups according to their functions: including rubber biosynthesis, stress- or defence-related responses, protein processing and folding, signal transduction and cellular transport. In addition to well-known rubber biosynthesis-related proteins such as rubber elongation factor (REF), small rubber particle protein (SRPP) and cis-prenyl transferase (CPT), many proteins were firstly identified to be on the rubber particles, including cyclophilin, phospholipase D, cytochrome P450, small GTP-binding protein, clathrin, eukaryotic translation initiation factor, annexin, ABC transporter, translationally controlled tumour protein, ubiquitin-conjugating enzymes, and several homologues of REF, SRPP and CPT. A procedure of multiple reaction monitoring was established for further protein validation. This comprehensive proteome data of rubber particles would facilitate investigation into molecular mechanisms of biogenesis, self-homeostasis and rubber biosynthesis of the rubber particle, and might serve as valuable biomarkers in molecular breeding studies of H. brasiliensis and other alternative rubber-producing species. © 2013 Springer Science+Business Media Dordrecht.


PubMed | Rubber Research Institute of China and Chinese Academy of Agricultural Sciences
Type: | Journal: Plant physiology and biochemistry : PPB | Year: 2017

Dendrobium hybrids orchid are popular throughout the world. They have various floral color and pigmentation patterns that are mainly caused by anthocyanins. It is well established that anthocyanin biosynthesis is regulated by the interplay between MYB and bHLH transcription factors (TF) in most plants. In this study, we identified one R2R3-MYB gene, DhMYB2, and one bHLH gene, DhbHLH1, from a Dendrobium hybrid. Their expression profiles were related to anthocyanin pigmentation in Dendrobium petals. Transient over-expression of these two TF genes showed that both DhMYB2 and DhbHLH1 resulted in anthocyanin production in white petals. The interaction between the two TFs was observed invitro. In different Dendrobium hybrids petals with various pigmentations, DhMYB2 and DhbHLH1 were co-expressed with DhDFR and DhANS, which are regarded as potential regulatory targets of the two TFs. In flowers with distinct purple lips but white or yellow petals/sepals, the expression of DhbHLH1 was only related to anthocyanin accumulation in the lips. Taken together, DhMYB2 interacted with DhbHLH1 to regulate anthocyanin production in Dendrobium hybrid petals. DhbHLH1 was also responsible for the distinct anthocyanin pigmentation in lip tissues. The functional characterization of DhMYB2 and DhbHLH1 will improve understanding of anthocyanin biosynthesis modulation in Dendrobium orchids.


Huang G.Q.,Rubber Research Institute of China
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji | Year: 2010

In order to enrich gene encoding region of Hevea brasiliensis, a methylation filtration library was constructed using Escherichia coli McrBC restriction-modification system. The titers of the non-amplified library and the amplified library were 2.6×106 pfu/ml and 9.0×109, respectively. The rate of positive clones was 86.4%. The lengths of inserted DNA sequence ranged from 1 kb to 2.5 kb and the average size of inserts was 1.2 kb. One hundred clones were selected randomly for sequencing, resulting in splicing out of 81 non-redundant sequences, including 6 contigs and 75 singlets. The redundancy was 17.35%. Blast analysis showed that 39.5% of non-redundant sequences were homologous with the Nr database, 14.81% with the EST database, and 32.1% were unknown sequences. Some sequences were related genes for flowering, insect and disease resistance. Therefore, the rubber tree methylation library is helpful for discovery and cloning of functional genes.


Wang L.-F.,Rubber Research Institute of China | Chen Y.-Y.,Rubber Research Institute of China
Pakistan Journal of Botany | Year: 2013

A new wide leaf rice mutant with high yield in the field was identified which has the leaf width about 1.5-2 folds more than that of wild-type. It possesses fewer, larger, upright leaves per plant. The chlorophyll contents of leaves were significantly higher than those of wild-type. The maximal PSII (Photosystem II) photochemistry (Fv/Fm), the electron transfer rate (ETR) and photochemical quenching (qP) were lower than those of wild-type, respectively. Because of great decrease of non-photochemical quenching (NPQ), the efficiency of excitation energy trapping by open PSII reaction centers in the light-adapted state (Fv'/Fm') was higher than that of wild-type. Low temperature fluorescence analysis showed that wide leaf mutant assigned more excited energy to PSI than to PSII. These results indicated that wide leaf mutant was better in the distribution of energy among 2 photosystems, and increase the efficiency of light utilization because of increase of chlorophyll contents. Furthermore, the SOD enzyme activities and MDA content of wide leaf mutant were nearly 44.4%, and 50.8% of those of wild-type, respectively. The low SOD and MDA contents indicated that this wide leaf mutant had high photosynthetic efficiency and its membrane structure did not probably affected(delete) by oxidation stress.


Wang L.F.,Rubber Research Institute of China | Chen Y.Y.,Rubber Research Institute of China
Photosynthetica | Year: 2011

An early senescence (es) mutant of rice Oryza sativa L. with progressing death of most of leaves before heading stage was identified in the field in Hainan province. After tillering stage, the brown striations were found in the base of green leaves randomly, and then expanded to whole leaves. No fungi, bacteria, and viruses were detected in the brown striations suggesting that it was a genetic mutant. The ultrastructure of leaf cells at the site of brown striations showed breakdown of chloroplast thylakoid membrane structures and other organelles, and condensation of the cytoplasm at severe senescence stage. The photosynthetic activity and chlorophyll (Chl) contents decreased irreversibly along with leaf senescence process. © 2011 Springer Science+Business Media B.V.

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