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Rastegarnia A.,Islamic Azad University at Urmia | Shahverdi A.,Royan Institute for Reproductive Biomedicine Research Center | topraggaleah T.R.,Islamic Azad University at Urmia | topraggaleah T.R.,Royan Institute for Reproductive Biomedicine Research Center | Shafiepour V.,Buffalo Breeding and Extension Training Center
Comparative Clinical Pathology | Year: 2014

The objective of the present study was to compare the quality of frozen buffalo semen processed in tris-citric egg yolk extender with two different commercially available soybean lecithin-based extenders. Split pooled ejaculates, possessing more than 70 % visual sperm motility were divided in three aliquots and diluted in AndroMed®, Bioxcell®, or tris-citric egg yolk (TCE) extenders. Post-thaw motion characteristics, viability, plasma membrane integrity, acrosome morphology, and DNA integrity of buffalo sperm were studied after thawing and incubation for 4 h. Results demonstrate that total motility in frozen-thawed semen processed in TCE extender were similar when compared with AndroMed® or Bioxcell® extenders (P > 0.05). Kinematic parameters, such as average path velocity and linearity index in the soybean lecithin-based extenders were comparatively superior to TCE extender. After 4 h of incubation, proportions of overall and progressive motility decreased in all extenders but were comparatively superior in soybean lecithin-based extenders than TCE extender (P < 0.05). The proportion of post-thaw sperm viability, plasma membrane integrity and normal apical ridge remained similar in all extenders (P > 0.05). The post-thaw sperm viability was comparatively superior in soybean lecithin-based extenders than TCE after incubation at 37 °C for 4 h (P < 0.05). The type of extender had not been the significant effect on the percentage of spermatozoa with DNA damage after thawing and incubation for 4 h. In addition, our results suggest that soybean lecithin base extenders can be used as a substitute for tris-citric egg yolk extender in cryopreservation of buffalo semen. © 2013 Springer-Verlag London. Source


Ebrahimi B.,Tarbiat Modares University | Valojerdi M.R.,Tarbiat Modares University | Valojerdi M.R.,Royan Institute for Reproductive Biomedicine Research Center | Eftekhari-Yazdi P.,Royan Institute for Reproductive Biomedicine Research Center | And 2 more authors.
Journal of Assisted Reproduction and Genetics | Year: 2010

Purpose: The purpose of this study was to evaluate the effects of cryotop vitrification of sheep cumulus-oocyte complexes (COCs) on oocyte maturation, apoptotic gene expression and incidence of chromosomal abnormalities. Methods: Freshly isolated (control group) and vitrified-warmed COCs (cryotop group) were matured in vitro. The expression of pro- and anti-apoptotic genes was investigated by real-time PCR. The incidence of numerical chromosomal abnormalities was evaluated by cytogenetic analysis. Results: The mean percentage of oocytes in the cryotop and control groups that reached metaphase II was 49.25±3.01% and 51.94±2.7% respectively. The expression rates of pro- and anti-apoptotic genes were similar in both groups, whereas the incidence of numerical chromosomal abnormalities was higher in the cryotop group compared to the control group (42.5% vs. 20%, p<0.05). Conclusion: Although cryotop vitrification of COCs did not affect the incidence of oocyte maturation or apoptotic gene expression, significant deficiencies in the maintenance of oocyte chromosomal organization were seen. © 2010 Springer Science+Business Media, LLC. Source

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