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Nasr-Esfahani M.H.,Royan Institute for Animal Biotechnology
Methods in molecular biology (Clifton, N.J.) | Year: 2013

Sperm selection is an important part of the ICSI procedure. Routinely, sperm selection for ICSI is based solely on sperm morphology and motility. These latter parameters may not be sufficient to select sperm with intact chromatin. Therefore, sperm selected based on sperm functional characteristics may result in the most appropriate sperm for the ICSI procedure. The methodology explained below describes the selection of sperm based on the ability of sperm to bind solid-state hyaluronic acid as its receptor, present on mature sperm with intact chromatin. Source


Ghasemi-Mobarakeh L.,Islamic Azad University at Najafabad | Prabhakaran M.P.,National University of Singapore | Morshed M.,Isfahan University of Technology | Nasr-Esfahani M.H.,Royan Institute for Animal Biotechnology | And 5 more authors.
Journal of Tissue Engineering and Regenerative Medicine | Year: 2011

Among the numerous attempts to integrate tissue engineering concepts into strategies to repair nearly all parts of the body, neuronal repair stands out. This is partially due to the complexity of the nervous anatomical system, its functioning and the inefficiency of conventional repair approaches, which are based on single components of either biomaterials or cells alone. Electrical stimulation has been shown to enhance the nerve regeneration process and this consequently makes the use of electrically conductive polymers very attractive for the construction of scaffolds for nerve tissue engineering. In this review, by taking into consideration the electrical properties of nerve cells and the effect of electrical stimulation on nerve cells, we discuss the most commonly utilized conductive polymers, polypyrrole (PPy) and polyaniline (PANI), along with their design and modifications, thus making them suitable scaffolds for nerve tissue engineering. Other electrospun, composite, conductive scaffolds, such as PANI/gelatin and PPy/poly(ε-caprolactone), with or without electrical stimulation, are also discussed. Different procedures of electrical stimulation which have been used in tissue engineering, with examples on their specific applications in tissue engineering, are also discussed. Copyright © 2011 John Wiley & Sons, Ltd. Source


Kuentz P.,University of Strasbourg | Kuentz P.,Center Hospitalier University | Vanden Meerschaut F.,Ghent University | Elinati E.,University of Strasbourg | And 10 more authors.
Human Reproduction | Year: 2013

STUDY QUESTION: Does DPY19L2 status influence intracytoplasmic sperm injection (ICSI) outcomes with or without assisted oocyte activation (AOA)?SUMMARY ANSWERDPY19L2 mutations have no major impact on ICSI outcomes in globozoospermic patients.WHAT IS KNOWN ALREADYGlobozoospermia is a rare and severe teratozoospermia characterized by round-headed spermatozoa lacking an acrosome. Recently, it has been shown that DPY19L2 mutations can be found in a vast majority of, but not all, globozoospermic patients (66.7%). These patients suffer from primary infertility due to a sperm-related oocyte activation deficiency secondary to the absence of an acrosome that can be overcome by the application of AOA.STUDY DESIGN, SIZE, DURATIONCohort study, retrospective, 34 patients, 83 cycles.MATERIALS, SETTING, METHODSClinical and biologic data were collected from 29 patients mutated for DPY19L2 and 5 non-mutated patients. In total, 35 ICSI cycles using AOA and 48 conventional ICSI cycles were included in the analysis. Patients were divided into groups according to whether or not they were mutated for DPY19L2 and whether or not they received AOA. MAIN RESULTS AND THE ROLE OF CHANCE: Regardless of the presence of a DPY19L2 mutation, the fertilization rates with AOA are restored to normal when compared with conventional ICSI in our cohort of globozoospermic patients. Also, when performing ICSI plus AOA, both mutated and non-mutated cases have similar positive hCG rates, ongoing pregnancy rates and live birth rates per transfer. On the contrary, the fertilization rate in globozoospermic patients using conventional ICSI is correlated with the presence of a DPY19L2 mutation, with slightly better, although still very low, fertilization rates in patients carrying a DPY19L2 mutation. Nevertheless, when performing conventional ICSI, both mutated and non-mutated cases have similar very low positive hCG rates, ongoing pregnancy rates and live birth rates per transfer.LIMITATIONSA limitation of this study is the low number of included non-mutated cases. WIDER IMPLICATIONS OF THE FINDINGS: We propose a pathway for the clinical management of globozoospermic patients depending on the phenotype that includes several diagnostic and therapeutic steps. STUDY FUNDING/COMPETING INTEREST(S): None. © 2013 The Author. Source


Ostadsharif M.,Islamic Azad University at Khorasgan | Ghaedi K.,Royan Institute for Animal Biotechnology | Ghaedi K.,University of Isfahan | Hossein Nasr-Esfahani M.,Royan Institute for Animal Biotechnology | And 5 more authors.
Differentiation | Year: 2011

Peroxisomal matrix protein is mainly expressed in heart, skeletal muscle, and brain tissues. To study the expression of peroxisomal protein (PEP) during neurogenesis, we employed mouse embryonic carcinoma cells (P19) and embryonic stem cells (mESCs) as an in vitro model for neural differentiation by retinoic acid (RA) induction. Expression pattern of PEP was investigated under distinct steps of differentiation by RT-PCR and real-time PCR. The results revealed that expression of PEP transcripts was markedly increased after the RA treatment at embryoid body and neural stages. Therefore, we concluded that PEP might be involved in the early process of neurogenesis, which needs further verification. © 2010 International Society of Differentiation. Source


Taghipour Z.,Royan Institute for Animal Biotechnology | Taghipour Z.,Isfahan University of Medical Sciences | Karbalaie K.,Royan Institute for Animal Biotechnology | Kiani A.,Royan Institute for Animal Biotechnology | And 6 more authors.
Stem Cells and Development | Year: 2012

Regarding both the neural crest origin and neuronal potential of stem cells from human exfoliated deciduous teeth (SHED), here, we assessed their potential in addition to neural induced SHED (iSHED) for functional recovery when transplanted in a rat model for acute contused spinal cord injury (SCI). Following transplantation, a significant functional recovery was observed in both groups relative to the vehicle and control groups as determined by the open field locomotor functional test. We also observed that animals that received iSHED were in a better state as compared with the SHED group. Immunohistofluorescence evaluation 5 weeks after transplantation showed neuronal and glial differentiation and limited proliferation in both groups. However, myelin basic protein and chondroitin sulfate proteoglycan NG2-oligodendrocyte markers-were increased and glial fibrillary acidic protein-astrocyte marker-was decreased in the iSHED group in comparison with the SHED group. These findings have demonstrated that transplantation of SHED or its derivatives could be a suitable candidate for the treatment of SCI as well as other neuronal degenerative diseases. © 2012 Mary Ann Liebert, Inc. Source

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